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INDONESIA
Jurnal Sain Veteriner
Published by Universitas Gadjah Mada
ISSN : 012660421     EISSN : 24073733     DOI : -
Core Subject : Health,
Veterinary
Arjuna Subject : -
Articles 23 Documents
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Search results for , issue "Vol 43, No 1 (2025): April" : 23 Documents clear
Comparison of Morphology and Protein Profile of Acetone and PBS-Fixed Toxoplasma gondii Tachyzoites for Detection of IgG and IgM Seropositivity VALINATA, SISCA; Cahyaningsih, Umi; Nugraha, Arifin Budiman; Kurniawati, Dyah Ayu; Desem, Muhammad Ibrahim; Fong, Sulinawati
Jurnal Sain Veteriner Vol 43, No 1 (2025): April
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.103155

Abstract

Toxoplasma gondii is an obligate intracellular protozoan that causes zoonotic diseases. The prevalence of toxoplasmosis in humans in Indonesia ranges from 9.7-70%. The parasite is difficult to detect in tissues; therefore, serological testing is the most common method for detecting antibodies against Toxoplasma. Antibodies are often used as the testing criteria for IgG and IgM antibodies. Previous research has shown that agglutination testing with acetone-fixed tachyzoites was only positive for acute infection. The aim of this study was to examine microscopic changes in acetone-fixed tachyzoites, determine their ability to detect IgG and IgM seropositivity, and detect specific proteins for IgG and IgM against toxoplasmosis. Tachyzoites were fixed with acetone (A) and PBS (P). After fixation, tachyzoites were prepared to observe the morphology and sonicated to obtain Soluble Toxoplasma Antigen (STA). STA was then subjected to SDS page and western blotting. The addition of aseton resulted in morphological and protein changes. Although changes occur, acetone-fixed tachyzoites can still react with IgG and IgM seropositivity. Protein bands that can be used as IgG seropositive markers in western blot testing are bands measuring 20, 24, 27, 73, and 110 kDa, and the combination of acetone fixation with anti-goat IgM conjugate will result in seropositive bands.
Histomorfometri Ginjal Tikus Putih (Rattus norvegicus L.) Strain Sprague Dawley Setelah Pemberian Doksisiklin dan Ekstrak Etanol Bunga Telang (Clitoria ternatea L.) Ariyanto, Hana Fadhillah; Kasiyati, Kasiyati; Sitasiwi, Agung Janika
Jurnal Sain Veteriner Vol 43, No 1 (2025): April
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.103306

Abstract

Doksisiklin merupakan antibiotik yang digunakan untuk mengatasi infeksi. Doksisiklin dengan dosis berlebih dapat menimbulkan radikal bebas yang mampu menyebabkan toksik bagi tubuh. Bunga telang (Clitoria ternatea L.) merupakan tanaman yang mengandung senyawa antioksidan, seperti flavonoid, antosianin, asam fenolik, prosianidin, dan flavonol glikosida. Antioksidan yang terkandung di dalam bunga telang berfungsi dalam menetralisir radikal bebas dalam tubuh. Tujuan dari penelitian ini, yaitu menganalisis histomorfometri ginjal tikus putih (Rattus norvegicus L.) strain Sprague Dawley setelah pemberian doksisiklin dan ekstrak etanol bunga telang (Clitoria ternatea L.). Tikus diaklimatisasi selama 1 minggu dan diberi perlakuan selama 2 minggu. Kelompok perlakuan yang diujikan pada penelitian ini, yaitu P0 (1 mL aquades), P1 (1 mL larutan doksisiklin), P2 (1 mL larutan ekstrak etanol bunga telang), P3 (1 mL larutan doksisiklin dan 1 mL larutan ekstrak etanol bunga telang). Hasil uji ANOVA satu arah menunjukkan bahwa hasil berbeda tidak nyata (P>0,05) terhadap diameter glomerulus, jarak ruang kapsula Bowman, tebal epitel tubulus kontortus distal, tebal epitel tubulus kontortus proksimal, bobot ginjal, dan konsumsi air minum pada tikus setelah perlakuan. Kesimpulan dari penelitian ini, yaitu pemberian doksisiklin dan ekstrak etanol bunga telang tidak memberikan dampak negatif pada struktur histologi ginjal tikus putih.
DESAIN PRIMER BERDASARKAN GEN MT-12S rRNA UNTUK MENDETEKSI CEMARAN DAGING BABI PADA PRODUK OLAHAN ASAL DAGING SAPI DENGAN METODE MULTIPLEKS-PCR Sitompul, Yeremia Yobelanno; Artanto, Sidna
Jurnal Sain Veteriner Vol 43, No 1 (2025): April
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.53197

Abstract

The case of adulteration of pork in beef products, which causes fretfulness especially for muslims, is still found in Indonesia. Multiplex Polymerase Chain Reaction (Multiplex PCR) can help detecting the adulteration of pork in beef products. This method needs multiple specific primers to amplify several targeted DNA mixture in single step reaction. This study aimed to design specific primers that can be utilized to detect the adulteration of pork in beef products using Multiplex PCR method.Firstly, primers were designed in silico using bioinformatic tools which were MegAlign, Seqbuilder, and AmplifX. This design was based on mt-12s rRNA gene. The length of amplified fragment using the designed primers for cow was 91 bp, while the pig was 230 bp. Secondly, the designed primers were tested to amplify DNA of cow and pig from raw beef, pork, mixture of beef and pork, and twelve samples of their products (meatballs) to assess their ability to amplify targeted DNA spesifically.The result showed that the designed primers could amplify the targeted DNA specifically from raw beef, pork, mixture of both meat, and their products. Therefore, the designed primers evidently can be used to detect adulteration of pork in beef by Multiplex PCR method.

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