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Jurnal Sain Veteriner

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Published by Universitas Gadjah Mada

ISSN : 012660421 EISSN : 24073733 DOI : -

Core Subject : Health,

Veterinary

Arjuna Subject : -

Articles 824 Documents

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Immunocytochemical Study on Blood and Organ Suspension of Tiger Grouper (Epinephelus fuscoguttatus) Infected with Field Isolate of Viral Nervous Necrosis Artanti Tri Lestari; Putu Eka Sudaryatma
Jurnal Sain Veteriner Vol 32, No 1 (2014): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

One potential marine cultures that have been developed and started to show the international market is grouper. Grouper culture can not be separated from factors that can affect disease and thwart cultivation. One of the diseases that has been reported by researchers is viral nervous necrosis (VNN) causing mass mortality in fish, especially grouper larvae and juvenile stadia. Laboratory of Balai KIPM kelas l Denpasar develop rapid diagnostic techniques, precise and accurate test using immunocytochemistry of blood and organs as one of the initial inspection. Tiger grouper sized 150-300 g as much as 50 and acclimatized, then 10 fishes used as controls, 40 fishes were injected with inoculum VNN 101.5 reared without water replacement cycle for ten days. Clinical observation and organ sampling performed 12 hours post-infection and consecutive every 12 hours. Blood samples and organs were collected for immunocytochemical (streptavidin-biotin) and a confirmatory test using RT - PCR using kit IQ -2000 VNN. Immunocytochemistry and RT-PCR showed positive results against VNN blood smears and suspensions organs of grouper fish with 24 hours post-infection . Based on the test results, the immunocytochemistry test on the blood and organ suspensions can be used as a detection technique VNN which is rapid, precise and accurate.

Inhibitory Effect of Extract Granule of Earthworms (Lumbricus rubellus) on the Pathogenic Bacteria In Vitro Lusty Istiqomah; Ema Damayanti; Hardi Julendra; Dewi istika; Sri Winarsih
Jurnal Sain Veteriner Vol 32, No 1 (2014): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

The objective of this study was to determine the inhibition ability of the earthworm (L. rubellus) extract (ECT), dried earthworm extract (ECT-k), and granule earthworm extract (ECT-g) as poultry feed additive against some pathogenic bacteria. Antibacterial activity was performed using diffusion method against Escherichia coli, Salmonella pullorum, Pseudomonas aeruginosa, and Staphylococcus aureus in vitro. In the present study, the concentrations of ECT, ECT-k, and ECT-g in nutrient broth (NB) media tested were consisted of treatments A: 0%, B: 0.26%, C: 0.52%, D: 0.78% and E: 1.04% (g/vol) respectively. The results of the in vitro study showed that started from ECT level 0.26% inhibited (P <0.05) growth of P. aeruginosa and S. aureus, while ECT level 0.52% inhibited (P <0.05) E. coli and S. pullorum which proportional to the increased in concentration. ECT-k level 0.26% inhibited (P <0.05) growth of E. coli and S. aureus, while ECT-k level 0.52% inhibited (P <0.05) P. aeruginosa, and ECT-k level 1.04% inhibited (P<0.05) growth of S. pullorum. ECT-g level 0.26% inhibited (P <0.05) growth of S. pulorum, while ECT-g level 0.52% inhibited (P <0.05) S. aureus and ECT-g level 1.04% inhibited (P<0.05) growth of P. aeruginosa. There were no antibacterial action (P>0.05) of ECT and ECT-t against S. pullorum. Diameter of inhibition zone for 24 hours showed that S. aureus was the most sensitive bacterium to ECT and ECT-k, and S. pullorum was the most sensitive bacterium to ECT-g.

The In Vitro Study: Anti Aeromonas hydrophila of Ethanol Extract of Kamboja Leaves (Plumeria alba) Ikrom .; Denok Asih T.R.; Reni Wira A.; Bintang Perkasa B; Rafika Tiara N.; Wasito .
Jurnal Sain Veteriner Vol 32, No 1 (2014): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Motile aeromonas septicemia (MAS) caused by Aeromonas hydrophila could attack seriously freshwater fish in Indonesia. Its mortality rate was reported to be 100% on fish population. According to the Commission of Healthy Fish and National Environment, the MAS has been determined as one of major diseases in Indonesia. However, its therapy using antibiotics is still difficult because the MAS has so many different strains and can be resistant to drugs. If the antibiotics given over the maximal limit this will be able to make the fish (food products) is unsafe for human consumption. One effort to do is by using a traditional herbal medicine. Kamboja leaves is reported to contain flavonoid as antibacterial agent. Therefore, this research is aimed to determine the effectiveness of kamboja leaves extract as antibacterial agent using diffusion and dilution method in vitro. Inhibition zone of diffusion method and bacterial growth are observed by diffusion and dilution approaches, rescpectively. The results of the present study showed that 8% concentration of kamboja leaves extract was good enough to prevent bacterial growth by dilution method, whereas by diffusion method, it needs 100% of the extract concentration. The zones of growth inhibition is 1.307 cm cm which is almost the same as the diameter (1,637 cm.) achieved by using 30 µg tetracycline. The zones of bacterial growth inbihition due to the extract still persists on the day 10. But, for the antibiotic, the the zones of bacterial growth inbihition is already faded starting from the day 4. Therefore, it is concluded that kamboja leaves extact is more potent than that of antibiotic (tetracycline) in inhibiting bacterial growth in vitro.

Developmental Anatomy of Cerebellum of Long-Tailed Macaque (Macaca fascicularis) at the First Trimester of Gestation Tri Wahyu Pangestiningsih; Irene Linda Megawati Saputra; Agnya Sinung Suminar
Jurnal Sain Veteriner Vol 32, No 1 (2014): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Long tailed macaque was one of animal models in biomedical research because it has many similarities with humans, both anatomical and physiological properties. There were many research about cerebellum associated with its role in the coordination of muscle activity. Understanding of normal development of cerebellum long tailed macaque may help to understand about the development in human cerebellum and its abnormalities. Embryonic and fetal brain samples were obtained through caesarean section and were then made for histological preparation stained with cresyl violet. Staining results were observed using a microscope with a digital camera. Images obtained are processed by graphics software Adobe Photoshop CS 8.0. Cerebellum Macaca fascicularis Ed40 showed the isthmus and rhombic lip that were composed of ventricular layer, mantle layer, and marginal layer. Cerebellum Macaca fascicularis Fd55 showed future lobes and future fissures, but the cortex and medulla are not bounded clear. The cortex consisted of the external granular layer, neuroblast basket, and neuroblast stellate, while the medulla consisted of neuroblast deep cerebellar nuclei. From this research, we concluded that neurons were on stage of proliferation and migration in the embryo aged 40 days, then differentiated and migrated to form cortex cerebellum and deep cerebellar nuclei at the age of 55 days, but the development of the cerebellum was not fully completed yet.

Phylogenetic Analysis of Thymidin Kinase Gene Koi HerpesVirus (KHV) in Some Freshwater Fish Aquaculture Centers in East Java Budi Rianto Wahidi
Jurnal Sain Veteriner Vol 32, No 1 (2014): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Koi Herpesvirus ( KHV ) is a virus that infects goldfish and koi that resulted in mass death and cause economic and social losses. The use of Polymerase Chain Reaction (PCR) technique to detect KHV was done in nila and gurame. Proof that KHV could infect nila and gurame KHV following genetic variation in each definitive host and spreading area have not been done. Based on these problems, this research was conducted to study the genetic variability and genetic relationships between KHV isolates in some freshwater fish as the definitive host koi, komet , koki, nila and gurame that exist in the area of East Java. The results showed that clinically the gills were pale and hemorrage the end of the tail fin. Electrophoresis results showed that all samples from KHV infected koi, koki, komet, nila and gurame were all positive but the sequencing results for tilapia and carp could not be further identified using the BLAST program. Genetically, koi fish, koki and komet isolates were identical to GenBank isolates code KHV-GZ11 and Indo_0K02SS.

The Accuracy of Transrectal Ultrasonography Diagnostic for Examination of Ovarian Structures in Cattle Prabowo Purwono Putro
Jurnal Sain Veteriner Vol 32, No 2 (2014): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

The study aimed to determine the accuracy of ovarian follicle and corpus luteum measurements in vivo using ultrasonography diagnostic and in vitro after slicing of the ovaries. A total of 15 cows were examined using transrectal ultrasonography just before being slaughtered. Ultrasonographic examination was performed using 7.5 MHz transrectal tranducer on ovaries, measured dominant follicle and corpus luteum diameters. Follicleappeared echogenic, black with round shape. Corpus luteum, appeared as low echogenicity structure and grayish in color.Ovaries were taken out soon after animals slaughtered, soaked in 10% buffered formalin for 12 hours,then cut in thin slices and measured follicle and corpus luteum diameters. Results of follicle and corpus luteum diameter measurements in vitro and in vivo compared using two sample or paired t-test, correlation and regression analyses. The two methods of follicle and corpus luteum measurements had linear orrelation (follicle measurement, R2 = 0.933 , r = 0.966, n = 15, P < 0.05) (corpus luteum, R2 = 0.912, r = 0.955, n = 15, P < 0.05).Measurements of dominant follicle diameter and corpus luteum size manifested the real size of the two ovarian structures. Transrectal ultrasonography diagnostic on ovaries proved to be having high accuracy and reliability for identification, measurement of ovarian structure diameters, follicle and corpus luteum in cattle.

A Study of the Fruit Bat (Rousettus sp) Brain Anatomy as Natural Reservoir Wild Animal for the Rabies Virus Karina Mayang Sari; Siti Khomariyah; Nur Arofah; Sabrina Wahyu Wardhani; Tri Wahyu Pangestiningsih
Jurnal Sain Veteriner Vol 32, No 2 (2014): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Rousettus sp. (Fruit bat) is one type of fruit bats in Indonesia and act as a natural reservoir of rabies. Rabies is caused by a virus from genus Lyssavirus, family Rhabdoviridae, which attack central nervous system (CNS).The brain is an organ that is sensitive to rabies infection. The purpose of this study was to determine the anatomical structure of the fruit bat brain macroscopically. Five fruit bat were used in this study, they were anaesthetized using ketamine and xylazin. Animals were perfused using physiological saline and 10% buffered formalin. Brains were taken using tweezers after all the bones of the skull were separated. Analysis of macroscopic brain was done descriptively. The results showed that the fruit bat brain were generally divided into cerebrum, cerebellum and brain stem. Gyrus, sulcus and the paraflokulus lobes of the fruit bat brain were less developed than that of the dogs brain.

Cholesterol Levels In Bligon Goats During Estrous Cycle Nadia Kartikasari; Risa Ummami
Jurnal Sain Veteriner Vol 32, No 2 (2014): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

The study was aimed to assess the status of cholesterol levels during the estrous cycle in goats. The present study was conducted by using 4 female cycling bligon goats (Capra hircus), aged 2-3 years, weighed 27-45 kgs, and clinically healthy and Venous blood sampling was done on days 1 (estrus), 3-4 (early luteal), 10-16 (late luteal), 17-20 (proestrus) using a syringe and collected in tubes containing lithium heparin. Plasma wasseparated by centrifugation, put into 1.5 ml Eppendorf tube and stored at a temperature of -20° C until analysis performed. Cholesterol levels were measured by using spectrophotometric method. Cholesterol concentrations during the estrous cycle were analyzed by General Linear Model Repeated Measure. The average of cholesterollevels in bligon goats is 52.20 ± 11.11 – 64.50 ± 18.46 mg/dl. The results showed that the concentration of cholesterol was between phases during the estrous cycle was not significantly different (P>0.05). However, there was a decreasing tendency of cholesterol concentration at the early luteal phase (52.20 mg/dl) and at the late luteal (53.19 mg/dl) compared to the levels at the follicular phase (65.50 mg / dl at estrus and 59.22 mg / dl at proestrus ). It can be concluded that the plasma level of cholesterol does not change significantly during theestrous cycle.

Prevalency of Nematode in Bali Cattle at Manokwari Regency Muhammad Junaidi; Priyo Sambodo; Dwi Nurhayati
Jurnal Sain Veteriner Vol 32, No 2 (2014): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

This study was conducted from August to October 2006, a disease caused by worms digestive tract is one of the causes of the decline in beef production . The purpose of this study was to assess the number of infected cattle nematode, the nematode types and amount of each type of nematodes in Bali cattle in Manokwari. Based onobservations on the worm eggs, total of 23 individuals (44.23 %) of Bali cattle ( puppies and adults ) infected with nematodes. Most of the infected cow is a cow that as many as 16 puppies cattle (69.56 %), while the remaining 7 animals (30.44 %) is the mother cow. Types of nematodes that infect cattle puppies and adult cattle are Cooperiasp., Bunostomum sp., Mecistocirrus sp. and Trichuris sp. The frequency of attacks in a row is as much as 13 cattle Cooperia sp, Mecistocirrus sp as many as 10 head of cattle, as many as 8 sp bunostomum cows and Trichuris sp. as much as 5 cows. The highest number of eggs in a row is Bunostomum sp. (1520), Cooperia sp. (1280), Mecistocirrus sp. ( 1200) and Trichuris sp. (280). The average number of eggs per gr feces ( EPG ) of 4 species of parasites that infect a row is Bunostomum sp . (190 EPG), Mecistocirrus sp. (120 EPG) Cooperia sp. (98.46 EPG)and Trichuris sp. (56 EPG). As many as 44.23 % of Bali cattle in three districts (Masni, Oransbari dan Prafi) attacked nematodes which are predominantly cattle aged less than one year .

Stability of gag-ca gene of Jembrana Virus in pcDNA-ca recombinant plasmid vector in Escherichia coli DH5α which has been stored for eight years Baso Yusuf; Adyatma M Nur; Nur Fiska Yunitasari; Tri Ari Widiastuti; Endah Puspitasari
Jurnal Sain Veteriner Vol 32, No 2 (2014): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

The aim of this study was to determine the stability of gag-ca gene in a pcDNA-ca recombinant plasmid vector in Escherichia coli DH5α which has been stored for eight years using Polymerase Chain Reaction method (PCR). Gag-ca gene which codes mayor protein has been characterized as immunodominan antigen, and it also has positive reaction to animal antibody which infected by Jembrana virus, so it usually used as vaccine resources and serologic detection for Jembrana disease. The research was done by analyzing gag-ca gene of Jembrana virus in pcDNA-ca recombinant plasmid in Escherichia coli DH5α which has been stored for eightyears. The pcDNA-ca recombinant plasmid was purified from cultured Escherichia coli DH5α recombinant bacteria using "High Pure Plasmid Isolation Kit", then amplificated with “Pure Taq-Ready To Go PCR Kit” using specific primers. PCR products showed a positive result that pcDNA-ca recombinant plasmid contains gag-ca gene. PCR products identification using electrophoresis on agarose gel 1% showed that DNA stand apparently carried gag-ca gene at approximate amplicon 210 base pair (bp).

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