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Jurnal Sain Veteriner

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Published by Universitas Gadjah Mada

ISSN : 012660421 EISSN : 24073733 DOI : -

Core Subject : Health,

Veterinary

Arjuna Subject : -

Articles 824 Documents

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Respon Sistem Homeostasis Kalsium Tikus Ovariektomi yang Mengkonsumsi Kombinasi Calcitriol dengan Raloxifene Hartiningsih Hartiningsih; Devita Anggraeni
Jurnal Sain Veteriner Vol 34, No 1 (2016): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Normal range of calcium (Ca) level in the blood is maintained by Ca homeostasis in the intestinal, kidney and bone. The objective of this research was to study the response of calcium homeostasis system ovariectomized rats consuming combination of calcitriol and raloxifene by calcium balance study. Twenty five female Wistar rats at 8 weeks of age were divided into five groups of five: normal control (NK), ovariectomy control (OVK), ovariectomy+calcitriol (OVD ), ovariectomy+ raloxifene (OVR), and ovariectomy+calcitriol+ 20raloxifene supplementation (OVDR), of five each. Seven weeks after the surgery, each rats was placed into individual metabolic cages for Ca balance studies (Ca consumption, feces and urine Ca excretion, and intestinalCa absorption). In 5 to 8 days of the balance studies, the remaining food, urine, and feces were collected every day for Ca analyses. The results showed that Ca consumption, feces and urine Ca excretion, and intestinal Ca absorption of OVDR rats were higher than OVK rats. Consumption and feces Ca excretion of OVDR rats were higher than OVK rats which informed the decrease of estrogen hormone. Intestinal Ca absorption of OVDR rats was higher than OVK rats which informed the decrease of parathyroid hormone. Meanwhile, urine Ca excretionof OVDR rats was higher than OVK rats which informed the decrease of parathyroid hormone and estrogen. In conclusions, the response of calcium homeostasis system in ovariectomized rats consuming combination ofcalcitriol and raloxifene for 8 weeks was marked by increasing Ca absorption in intestine and Ca excretion in urine.

Keberadaan Anisakis typica (Anisakidae) dari Ikan Tongkol dan Ikan Layang dari perairan Sulawesi Barat Muhammad Dusil Hafid; Hilal Anshary
Jurnal Sain Veteriner Vol 34, No 1 (2016): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Anisakis (L3-stage) is a parasitic nematode commonly found in marine fish or squid serve as intermediate or paratenic host. The purpose of this research was to identify Anisakis larvae using PCR-RFLP and sequencing methods. A total of 30 individuals of each species: bullet tuna (Auxis rochei) and Indian scad(Decapterus russeli) were examined for presence of Anisakis spp. Fish samples caught by fishermen around Mamuju waters were purchased at a fish market. The fish was necropsied and the organs: liver, intestine wall, heart and muscle were removed and put on separated petri dishes and examined for parasites under a stereo microscope. Anisakis found was cleaned and fixed in 70% alcohol. Initial identification was based on ventriculus shape and presence of mucron to distinguish Anisakis type I and Anisakis type II. All Anisakis found belong to Anisakis type I. Ten Anisakis type I were isolated, cleaned and stored in microfuge tubes containing 70% alcohol. The parasite gDNA was extracted using Wizard genomic DNA extraction and purification kit (Promega).Species of Anisakis was determined by PCR-RFLP at ITS1-5.8S-ITS2 region using two restriction enzymes: Taq I and Hinf I and PCR-sequencing in mtDNA cox2 region. Analysis by PCR-RFLP showed that all Anisakis type Iexamined was in the same pattern as Anisakis typica. Sequencing in mtDNAcox-2 region and the phylogenetic analysis showed that all samples were in the same cluster as A. typica. Based on PCR-RFLP and sequencinganalysis, all Anisakis found in this study belong to A. typica.

Identifikasi Virus Avian Leukosis Sub Grup J pada Peternakan Ayam Petelur Komersial di Kabupaten Tangerang Tahun 2015 Risza Hartawan; Ni Luh Putu Indi Dharmayanti
Jurnal Sain Veteriner Vol 34, No 1 (2016): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Avian leukosis virus (ALV) is one of causing agents for neoplastic syndrome in commercial chicken farms. There are six sub groups of ALV in chicken including A, B, C, D and E. However, the sub group J (ALV-J) is the most alarmed and anticipated because of its severe economic repercussion. The objective of this study was to identify the presence of ALV-J in the commercial chicken farms in Tangerang District. Two identification methods were utilized including antigen capture enzyme-linked immunosorbent assay (ac-ELISA) technique and nested reverse transcriptase polymerase chain reaction (nRT-PCR) test. As results, the ac-ELISA test detected that most of 150 serum samples from 5 commercial layer farms were positive for ALV in about 86,67%. On the other hand, none of the 60 cloacal swab and 60 albumen samples was positive for presence ALV. Subsequently, testing of 45 serum samples from 5 farms using nRT-PCR showed that in about 20% of these samples were positive of ALV-J. These evidences indicated the circulation of ALV-J with other sub groups in thecommercial layer farms in Tangerang District.

Daya Hidup Spermatozoa Epididimis Kambing Peranakan Ettawa yang Dipreservasi dengan Pengencer Tris dan Bberbagai Konsentrasi Maltosa Muhammad Rizal; Dwi Sulistiowati; Abrani Sulaiman; Herdis Herdis; Insun Sangadji
Jurnal Sain Veteriner Vol 34, No 1 (2016): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Cauda epididymal spermatozoa could be used as an alternative source of gamete in the application of various reproductive technologies, since the spermatozoa is motile and has ability for fertilizing the oocyte. Theobjective of this research was to examine the effectivity of maltose in maintaining viability of ettawa crossbreed goat epididymal spermatozoa preserved at 3–5oC. Five testis with epididymides of ettawa crossbreed goat were obtained from slaughterhouse. Epididymal spermatozoa was collected by the combination of slicing, flushing and tissues pressure of cauda epididymides with physiological saline (0.9% NaCl). Collected-spermatozoa wasdivided in equal volume into three tubes and diluted with Tris extender containing 20% egg yolk (control), Tris extender + 0.3 g maltose/100 ml (M0.3), and Tris extender + 0.6 g maltose/100 ml (M0.6), respectively. Dilutedspermatozoa was stored in refrigerator at 3–5oC. Quality of diluted-spermatozoa including percentages of motile spermatozoa (MS) and live spermatozoa (LS) were evaluated every day during storage at 3–5oC for four days. Data were analyzed using completely randomized design with three treatments and five replicates. Means were compared significant difference test at 0.05 significant level. Results of this study showed that mean spermatozoaconcentration, percentage of MS, percentage of LS, and percentage of abnormal spermatozoa of ettawa crossbreed goat fresh epididymal spermatozoa were 3,220 million cell/ml, 70%, 81%, and 4.3%, respectively. At day-5 of storage, percentages of MS and LS for M0.3 (38 and 60.4%) and M0.6 (38 and 57.2%) were significantly (P<0.05) higher than control (32 and 55.4%). In conclusion, addition of 0.3 and 0.6% maltose in Tris extender could be maintained viability of ettawa crossbreed goat epididymal spermatozoa preserved at 3–5oC forthree days.

Karakterisasi Faktor-faktor Virulensi Staphylococcus aureus Asal Susu Kambing Peranakan Ettawa secara Fenotip dan Genotip Khusnan Khusnan; Wahyu Prihtiyantoro; Hartatik Hartatik; Mitra Slipranata
Jurnal Sain Veteriner Vol 34, No 1 (2016): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Staphylococcus aureus is a major cause of mastitis in large or small ruminants, and often manifested by subclinical mastitis in Peranakan Ettawa (PE) goats. Staphylococcus aureus in human can cause food borne disease. The research aimed to characterize the virulence factors of Staphylococcus aureus isolated from milk PE goats, phenotypic- and genotypically. Phenotypically characterization were determined through the pigmen assay as well as hydrophobicity, haemolysin, and hemaglutinin reaction. Polymerase chain reaction (PCR) analysis was used to detect 4 virulen genes including coa, clf, fnbA, and fnbB genes. The results of research showed that Staphylococcus aureus abled to produce white pigmen (35,7%), yellow pigmen g (57,1%), andorange pigmen (7,2%). Staphylococcus aureus showed α-hemolysis zone (35,7%), β-hemolysis (35,7%), dan γ-hemolysis (28,9%). Hydrophobicytic test revealed 14,3% Staphylococcus aureus isolates were hydrophobe and85,7% hydrophil. Staphylococcus aureus (85,7%) isolates abled to aglutinated sheep blood cells. Based on genotypic analysis of Staphylococcus aureus could be detected coa gene (92,8%), clf gene (64,3%), fnbA gene (78,6%), and fnbB gene (64,3%). Based on the phenotypic and genotypic characters, it can be concluded that Staphylococcus aureus are virulent strains. This information can be used as the basis for control mastitis in PE goats

Nano Spray TRISWHEAT (Teripang Super Wound Healing Agent) Penyembuh Luka Diabetes Mellitus yang Terinfeksi Bakteri MRSA (Methicillin Resistant Staphylococcus aureus) dengan Ekstrak Teripang Nada Hanifah; Yusuf Farid Achmad; Mellya Permatasari; Marista Kurniati; Ditya Tiwi Syafira; Siti Isrina Oktavia Salasia
Jurnal Sain Veteriner Vol 36, No 1 (2018): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Sea Cucumber (Stichopus sp) belongs to Holothuroidea family. Sea Cucumber has lot of uses in the medicine sector, such as: for wound healing, anti-biotic, anti-bacteria, anti-tumor, and anti-coagulation. Diabetes mellitus is a disease characterized by high blood sugar levels with metabolic disorders. This disease can cause wounds called gangrene This research has purpose to prove the influence of giving nano spray of sea cucumber extract within 14 days. In the in vitro examination found 40% concentration of sea cucumber extract which is effective to resist the growth of Methicillin Resistant Staphylococcus aureus bacteria and examined to wistar mouse which are already diabetic inducted with Streptozotocin (STZ) and hurt with punch biopsy with the diameter of 0,8 cm on its back and the wound is infected with bacteria which is intradermal injected. Divided into 3 groups : (K-) no medical treatment, nano spray of sea cucumber extract (T), Nano spray penicillin (K+) to wistar mouse towards the speed of diabetic mellitus wound healing which is infected by Methicillin Resistant Staphylococcus aureus bacteria. Parameter used to measure the rate of wound healing is wound size and hitopathology examination.After it’s regularly given the medicine, the result of nano spray sea cucumber extract 40 % concetration category treatment on wound healing process the diameter of wound on the 3rd day 0.8 cm , on the 7th day 0.4 cm and on the 14th day wound fully recover according to macros (wound size) and histopathology examination shows faster and better healing compared to treatment category of nano spray penicillin and control marked by score cell and lymphocyte in a small quantity.

Rekayasa Konsentrat Bekatul dengan Tepung Jantung Pisang Dapat Meningkatkan Berat Badan dan Menurunkan Kadar Kolesterol Kambing Dina Deviana; Nur Prabowo Dwi Cahyo; Dwi Retna Kumalaningrum; Widya Ayu Kusuma; Fahlevi Lailiyah; Muhammad Thohawi Elziyad Purnama
Jurnal Sain Veteriner Vol 36, No 1 (2018): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Tujuan: tujuan dari penelitian ini yaitu untuk meningkatkan berat badan dan menurunkan kadar kolesterol pada kambing. Metode Penelitian: metode penelitian yang digunakan yaitu dengan pemberian feed additive pakan konsentrat bekatul dengan tepung jantung pisang menggunakan metode rancangan acak lengkap (RAL). Penelitian ini menggunakan 5 perlakuan dengan 4 pengulangan, jumlah hewan coba yaitu kambing 20 ekor. Kambing yang kita gunakan berjenis kelamin jantan dengan umur 1,5 tahun. Pengumpulan data diambil dari peningkatan berat badan dan kadar kolesterol kambing. Hasil: pemberian feed additive konsentrat bekatul dengan tepung jantung pisang dapat meningkatkan berat badan dan menurunkan kadar kolesterol pada kambing. Nilai keberhasilan dengan skala P<0.05, menunjukan adanya perbedaan nyata pada tiap perlakuan yang dilakukan. Perlakuan terbaik yang dilakukan pada penelitian ini yaitu pada P2 dengan kenaikan berat badan 2,95 ons/hari dan kadar kolesterol dalam dua minggu turun sebanyak 87,75. Kesimpulan: pada penelitian ini dapat disimpulkan bahwa pada tiap perlakuan memiliki perbedaan nyata untuk kenaikan berat badan dan penurunan kadar kolesterol kambing. Dapat disimpulkan juga bahwa penambahan pakan konsentrat bekatul dan tepung jantung pisang dapat meningkatkan berat badan dan menurunkan kadar kolesterol pada kambing.

Dekontaminasi Mycobacterium avium subspecies paratuberculosis pada feses menggunakan beberapa jenis desinfektan Ika Suharti; Ni Luh Putu Ika Mayasari; Fachriyan Hasmi Pasaribu
Jurnal Sain Veteriner Vol 36, No 1 (2018): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Paratuberculosis or Johne’s Disease is a granulomatous enteritis chronic disease of domestic and wild ruminants caused by infection of Mycobacterium avium subspecies paratuberculosis. The disease commonly infects dairy cattle with clinical signs of chronic diarrhea, decreasing body weight, low milk production, oedema, anemia and occasionally infertility. The basic procedure in order to control Paratuberculosis in farms is to do a good and proper handling of animal faecal. Disinfection of animal environments such as pens, faecal, sewerage and sewage are important in prevention of transmission of this disease. The purpose of this research is to determine specific disinfectan and dosage for Mycobacterium avium subspecies paratuberculosis decontamination in cattle feces so it can be applied as disease control measures. Cow's feces were contaminated with MAP 105CFU/ml and treated with ammonium quartener, phenolic and formaldehyde disinfectant doses 10%, 15% and 20%. The effectiveness of the disinfectant was tested based on MAP identification using Löwenstein-Jensen culture medium and nested Polymere Chain Reaction(PCR). The results showed 15% and 20% doses of formaldehyde disinfectants efective to decontaminate Mycobacterium avium subspecies paratuberculosis in catle feces.

Kertas Saring sebagai Media Transpor Darah untuk Pemeriksaan Antibodi Rabies Retno Wijayanti; Retno Damayanti; Sri Murtini
Jurnal Sain Veteriner Vol 37, No 2 (2019): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Rabies is a zoonotic disease. Rabies protection level detection was performed using antibody titration. Blood sampling activities in the field require special handling to avoid blood lysis, the sample delivery requires a cold chain with a stable temperature. Alternative method forwhole blood sample delivery using filter paper were carried out on 48 samples from susceptible animals (dogs and cats) transpored through the Soekarno Hatta Agricultural Quarantine Center. This study was designed to investigate the feasibility of filter paper sampling of blood at temperature of 26 °C to detect of rabies antibodies using indirect method of ELISA. The results of statistical analysis of Randomized Block Design and Tukey's test showed that the antibody titres of whole blood extracted from filter paper diluted in the PBS T 100 μl were equivalent to antibody titres of serum. Assesment of filter paper capability has sensitivity and specificity as much as 96% and 76%, positive predictive value of 67%, negative predictive value of 94% and reliability level of filter paper were 0.5 is moderate category. This indicates that the filter paper can be used as an alternative method of blood transport medium for rabies ELISA test.

Kajian Equine Infectious Anemia pada Kuda Impor di Bandar Udara Internasional Soekarno-Hatta Marlefzena Marlefzena; Sri Murtini; Joko Pamungkas
Jurnal Sain Veteriner Vol 36, No 1 (2018): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Equine Infectious Anemia (EIA) disease is caused by EIA Virus (EIAV) from genus Lentivirus, subfamily Orthoretrovirinae, family retroviridae. This virus causes a persistent infection and potentially fatal in Equidae. There is no scientific literature yet for this disease in Indonesia. In order to determine this disease in our country, it is necessary continuing laboratory detection in imported Equidae and conducted further surveillance in Equidae throughout Indonesia. This research is aimed (1) to detect of any antibodi titre of EIAV in imported horse, (2) To get information about the relationship between laboratory result with the secondary datas of origin country. The EIAV antibody detected by indirect enzyme-linked immunosorbent assay (indirect ELISA). The secondary datas were collected from Health Certificate of origin country, World Animal Health Information System (WAHIS) Interface OIE and literatures. Total of 133 blood samples were collected from imported horse, ex-imported horse and lokal breed horse. The result showed that all of imported horse, ex-imported horse and lokal breed horse were negative antibody against EIAV. The initial information on Equine Infectious Anemia indicated that health requirement in imported horse has fulfilled, so that imported horse was free from EIAV.

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