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THE EFFICACY OF AVOCADO, MUNG BEAN SPROUTS, AND HOLY BASIL HERB COMBINATION (JAMU ATOKE) ON THE HEALTH AND REPRODUCTIVITY OF ADULT FEMALE RATS Andriyanto Andriyanto Andriyanto; Leliana Nugrahaning Widi; Hamdika Yendri; Kharisma Mardathilah; Diky Yuliansah; Firda Agustin; Aulia Andi Mustika; Wasmen Manalu
Jurnal Kedokteran Hewan Vol 16, No 4 (2022): December
Publisher : Universitas Syiah Kuala

The purpose of this study was to examine the efficacy of a herbal combination of avocado, mung bean sprouts, and holy basil (Jamu ATOKE) in optimizing the reproductive health of adult female rats. Eighteen female Sprague Dawley rats aged 9-10 weeks old weighing around 180-250 g, and had not been pregnant previously were equally and randomly divided into 3 groups: one control group and two treatment groups (n= 6 rats per group). The treatment groups received Jamu ATOKE with a concentration of 2.5 and 5%, respectively. Jamu ATOKE was added to the drinking water and consumed by the rats for 30 days prior to pregnancy. Reproductivity (pregnancy success, estrogen and progesterone hormone concentrations), and health performance (body weight gain, food, and water consumption, motor activity, red blood cell (RBC) count, white blood cell (WBC) count and differentials, SGPT, SGOT, ureum, and creatinine) of rats were observed. The results showed that groups receiving the Jamu ATOKE had better reproductive and health performance compared to those of the control group. The administration of Jamu ATOKE can significantly increase the concentration of estrogen and progesterone in rats (P0.05). It can also improve the fertility and health of the female rats prior to pregnancy.

SPECTROSCOPIC ANALYSIS OF A COMPOUND ISOLATED FROM THE ETHYL ACETATE FRACTION OF CALOPHYLLUM MACROPHYLLUM SCHEFF AND TOXICITY TESTS USING THE BSLT METHOD AGAINST ARTEMIA SALINA LEACH LARVAE Noni Zakiah; M Munira; Rini Handayani; R Rasidah; F Frengki
Jurnal Kedokteran Hewan Vol 16, No 4 (2022): December
Publisher : Universitas Syiah Kuala

The Calophyllum genus has been reported to be rich in bioactive compounds that have medicinal properties. The author will also report the results of the isolation of compounds obtained from the ethyl acetate fraction of the bark of the plant C. macrophyllum Scheff, a type of Calophyllum found in Mount Kerinci, Jambi province. This paper will analyze the spectroscopic data of these compounds which include UV, IR, GC-MS, 1HNMR and 13CNMR spectra which are amplified by HMQC and HMBC spectra. The first step in bioactivity testing is the toxicity test using the BSLT (Brine Shrimp Lethality Test) method using Artemia Salina Leach shrimp larvae. The results of the spectroscopic analysis obtained the chemical formula as a derivative of fatty acid methyl ester (methyl-octadec-14 enoic), in the form of a yellow liquid with a boiling point of 173-175°C. The results of the initial screening for bioactivity through the toxicity test of the BSLT method on Artemia salina Leach shrimp larvae obtained an LC50 value of 141.2 g/mL. This compound still has the potential to have certain bioactivity

HEPARIN AND HYPOTAURINE SUPPLEMENTATION IMPROVE THE FERTILIZATION RATE OF SHEEP OOCYTES MATURED IN MEDIA CONTAINING L-CARNITINE IN VITRO Rahmatullah Rahmatullah; Mohamad Agus Setiadi; Iman Supriatna
Jurnal Kedokteran Hewan Vol 16, No 4 (2022): December
Publisher : Universitas Syiah Kuala

This study to do examine the effectiveness of using heparin and hypotaurine to improve the in vitro fertilization rate. In first stage, oocytes were matured in the maturation medium supplemented with 0.3 mg/mL L-carnitine to improve nuclear maturation. In second stage, matured oocytes were fertilized with semen incubated in fertilization medium supplemented with 0.1 IU/mL heparin and 10 µM hypotaurine. The final concentration of sperm used was 5x106 spermatozoa/mL, and its interaction with oocytes was carried out for 12 hours in 5% CO2 incubator at 38.5° C. The results showed that percentage of oocytes that reached metaphase II was significantly higher (P0.05) in media supplemented with 0.3 mg/mL L-carnitine compared to control (88.2±4.9% vs 79.8±5.1%). Furthermore, fertilization rate was characterized by formation of 2 PN was significantly higher (P0.05) in media supplemented with heparin and hypotaurine than control (82.6±2.0% vs 73.3±2.4%). In conclusion, L-carnitine supplementation at dose of 0.3 mg/mL on maturation media was effective in increasing percentage of matured oocytes. The combination of 0.1 IU/mL heparin and 10 µM hypotaurine in fertilization medium was also successful in elevating the percentage of fertilized oocytes.

EFFECTIVENESS OF PROBIOTIC AND DIGESTIVE ENZYMES IN FERMENTED DIET ON GROWTH, FAT AND CHOLESTEROL CONTENTS OF BROILER Dr. M Aman Yaman
Jurnal Kedokteran Hewan Vol 16, No 4 (2022): December
Publisher : Universitas Syiah Kuala

ABSTRACTThe present study aims to determine the effect of fermented diet containing probiotics and digestive enzymes with different feed levels on growth, fat and cholesterol contents of broiler chickens. Evaluating the effectiveness of probiotics (P) and digestive enzymes (DE) in fermented diet on broiler was observed by 4 treatments: P0=control (fermented diet without P and ED) EP), P1(fermented diet contained 0.5% P only)), P2 (fermented diet contained 0.5% DE only), P3 (fermented diet contained 0.5% P and 1% DE) and P4 (fermented diet contained 1% P and 0.5% DE). A hundred DOCs of broiler chickens strain CP 707 were used (unsex) and it designed by a completely randomized design with 5 treatments, 4 replications and each replication consisted of 5 DOCs. The data obtained were analyzed by means of variance and Duncan's multiple range test. The results of present study showed that broiler fed on fermented diet containing P and/or ED with different levels had a significant effect (P0.05) on final body weight, weight gain, carcass weight, fat content on thigh muscle. Broiler fed on fermented diet contained 0.5% DE only (P2) resulted in better growth and weight gain than other treatments. However, fermented diet contained probiotics and digestive enzymes had no effect on fat and cholesterol contents in breast muscle. It was concluded that the addition of digestive enzymes in fermented diet formulated by local raw materials was very effective than probiotics in increasing body weight gain, carcass weight and prevented the increase in fat and cholesterol contents both in breast and thight muscles of broiler chicken.

GENETIC DIVERSITY OF IRAQI LOCAL GOAT BREEDS BY RAPD DNA MARKERS Awat Nooradin Yousif; Mohammed Abdalla
Jurnal Kedokteran Hewan Vol 16, No 4 (2022): December
Publisher : Universitas Syiah Kuala

Genetic diversity of three Iraqi local goat breeds (Black, Hybrid, and Meriz) was studied by using random amplified polymorphic DNA (RAPD) markers. Fifteen primers were used, they generated a total of 259 amplification products, from which 142 bands were polymorphic, revealing 55.29% polymorphism. It was found that the mean value of Nei’s gene diversity (h) and Shannon's ınformation index (I) were 0.1778 and 0.2546, respectively. According to the analysis of genetic distances among breeds, the highest genetic distance recorded between Black and Hybrid 0.5108, while the lowest genetic distance recorded between Hybrid and Meriz 0.1431. The phylogenetic dendrogram based on Nei’s genetic distance analysis was in agreement with their phenotypic similarities in which the tree separated the surveyed breeds into two clusters, the first one including Black goat breed, and the second cluster includes Hybrid and Meriz breeds. These results indicated that Black goat breed is most genetically distant from both Hybrid and Meriz breeds. Finally, the moderate value for both of polymorphism and genetic diversity which obtained from the present study indicate that these breeds are genetically different, which provide a good background about these breeds for genetic improvement and conservation of our local goat breeds in the future.

MORPHOFUNCTIONAL CHARACTERISTICS OF THE LOWER HINDLIMB AND FOOT MUSCLES OF BORNEAN ORANGUTAN (Pongo pygmaeus) Nicolas Edward Christanto Kartjito; Ikhsan Kadarusman; Savitri Novelina; Chairun Nisa’; Sri Rahmatul Laila; Danang Dwi Cahyadi; Nurhidayat Nurhidayat
Jurnal Kedokteran Hewan Vol 16, No 4 (2022): December
Publisher : Universitas Syiah Kuala

Orangutan is a great ape that has anatomical and physiological aspects similar to those of gorillas and chimpanzees, although orangutan is more arboreal than other great apes. This research aims to study the morphofunctional characteristics of the lower hindlimb and foot muscles of Bornean orangutan and its relation to the type of locomotion and daily behavior. This research was conducted on the right leg of a male orangutan by observing the morphology of the lower hindlimb and foot muscles and observing the behavior of the Bornean orangutan through video observations. The results showed that digital flexor muscles are more developed than plantar flexor muscles as an adaptation towards arboreal activities that require finger flexor movements such as gripping tree branches. Orangutans have rudimentary hallux, which is related by the less developed intrinsic muscle and indicated by the way orangutan grips tree branches dominantly using digits II-V. At a certain time, male Bornean orangutans are active on terrestrial that causes the digital extensor muscles to have more developed as an adaptation to terrestrial activity. It can be concluded the morphology of the lower hindlimb and foot muscles is in accordance to its function and behavior adaptation in arboreal and terrestrial.

THE REPRODUCTIVE CHARACTERISTICS OF GAYO BUFFALOES AS A LOCAL LIVESTOCK GENETIC RESOURCE IN THE MESIDAH SUBDISTRICT, BENER MERIAH REGENCY Mohd. Agus Nashri Abdullah; Tudung Mulie Gundala; Eka Meutia Sari
Jurnal Kedokteran Hewan Vol 16, No 4 (2022): December
Publisher : Universitas Syiah Kuala

This research aimed to identify the reproductive characteristics of Gayo buffaloes in the Mesidah Sub-district, Bener Meriah Regency. There were three locations of the current study namely Perumpaken Benjadi, Wer Tingkem, and Simpur, which were determined using the purposivesampling technique. A survey was used as the research method to obtain primary data and secondary data. Primary data were collected throughdirect interviews with buffalo livestock owners. They were asked a set of questions that had been prepared in a questionnaire. Secondary datawere obtained from related agencies in Bener Meriah Regency. The number of respondents was 92 from four villages in the Mesidah Sub-district,Bener Meriah Regency. Breeders as respondents were selected using a purposive sampling method, with minimum requirements that they hadraised two buffaloes that had given birth more than once and that the breeders had at least four years of farming experience. The research datawere analyzed using descriptive statistic. The results showed that the Gayo buffaloes in the Mesidah Sub-district, Bener Meriah Regency, had reproductive characteristics that met the standards. This can be seen from the obtained data which were almost the same as the average age of the reproductive characteristics of buffaloes in Indonesia. Gayo buffaloes in the Mesidah Sub-district experienced the first estrus (puberty) at 33.83 months old, first mating at 34.61 months old, and first calving at 45.29 months. The average gestation period was 10.51 months, the calving interval was 15.5 months, and the average service period was 3.66 months, while maintenance management was carried out extensively. Based on the results, it can be concluded that the reproductive characteristics of Gayo buffaloes in the Mesidah Sub-district, Bener Meriah Regency, still meet the standards. The average reproductive characteristics of Gayo buffaloes are generally the same as the average characteristics of other buffaloes in Indonesia.

GENETIC DIVERSITY OF IRAQI LOCAL GOAT BREEDS BY RAPD DNA MARKERS Yousif, Awat Nooradin; Abdalla, Mohammed
Jurnal Kedokteran Hewan Vol 16, No 4 (2022): December
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v16i4.28579

Genetic diversity of three Iraqi local goat breeds (Black, Hybrid, and Meriz) was studied by using random amplified polymorphic DNA(RAPD) markers. Fifteen primers were used, they generated a total of 259 amplification products, from which 142 bands were polymorphic,revealing 55.29% polymorphism. It was found that the mean value of Neis gene diversity (h) and Shannon's nformation index (I) were 0.1778 and 0.2546, respectively. According to the analysis of genetic distances among breeds, the highest genetic distance recorded between Black and Hybrid 0.5108, while the lowest genetic distance recorded between Hybrid and Meriz 0.1431. The phylogenetic dendrogram based on Neis genetic distance analysis was in agreement with their phenotypic similarities in which the tree separated the surveyed breeds into two clusters, the first one including Black goat breed, and the second cluster includes Hybrid and Meriz breeds. These results indicated that Black goat breed is most genetically distant from both Hybrid and Meriz breeds. Finally, the moderate value for both of polymorphism and genetic diversity which obtained from the present study indicate that these breeds are genetically different, which provide a good background about these breeds forgenetic improvement and conservation of our local goat breeds in the future.

MORPHOFUNCTIONAL CHARACTERISTICS OF THE LOWER HINDLIMB AND FOOT MUSCLES OF BORNEAN ORANGUTAN (Pongo pygmaeus) Kartjito, Nicolas Edward Christanto; Kadarusman, Ikhsan; Novelina, Savitri; Nisa, Chairun; Laila, Sri Rahmatul; Cahyadi, Danang Dwi; Nurhidayat, Nurhidayat
Jurnal Kedokteran Hewan Vol 16, No 4 (2022): December
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v16i4.28199

Orangutan is a great ape that has anatomical and physiological aspects similar to those of gorillas and chimpanzees, although orangutan is more arboreal than other great apes. This research aims to study the morphofunctional characteristics of the lower hindlimb and foot muscles of Bornean orangutan and its relation to the type of locomotion and daily behavior. This research was conducted on the right leg of a male orangutan by observing the morphology of the lower hindlimb and foot muscles and observing the behavior of the Bornean orangutan through video observations. The results showed that digital flexor muscles are more developed than plantar flexor muscles as an adaptation towards arboreal activities that require finger flexor movements such as gripping tree branches. Orangutans have rudimentary hallux, which is related by the less developed intrinsic muscle and indicated by the way orangutan grips tree branches dominantly using digits II-V. At a certain time, male Bornean orangutans are active on terrestrial that causes the digital extensor muscles to have more developed as an adaptation to terrestrial activity. It can be concluded the morphology of the lower hindlimb and foot muscles is in accordance to its function and behavior adaptation in arboreal and terrestrial.

THE REPRODUCTIVE CHARACTERISTICS OF GAYO BUFFALOES AS A LOCAL LIVESTOCK GENETIC RESOURCE IN THE MESIDAH SUBDISTRICT, BENER MERIAH REGENCY Abdullah, Mohd. Agus Nashri; Gundala, Tudung Mulie; Sari, Eka Meutia
Jurnal Kedokteran Hewan Vol 16, No 4 (2022): December
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v16i4.26335

This research aimed to identify the reproductive characteristics of Gayo buffaloes in the Mesidah Sub-district, Bener Meriah Regency. There were three locations of the current study namely Perumpaken Benjadi, Wer Tingkem, and Simpur, which were determined using the purposivesampling technique. A survey was used as the research method to obtain primary data and secondary data. Primary data were collected throughdirect interviews with buffalo livestock owners. They were asked a set of questions that had been prepared in a questionnaire. Secondary datawere obtained from related agencies in Bener Meriah Regency. The number of respondents was 92 from four villages in the Mesidah Sub-district,Bener Meriah Regency. Breeders as respondents were selected using a purposive sampling method, with minimum requirements that they hadraised two buffaloes that had given birth more than once and that the breeders had at least four years of farming experience. The research datawere analyzed using descriptive statistic. The results showed that the Gayo buffaloes in the Mesidah Sub-district, Bener Meriah Regency, had reproductive characteristics that met the standards. This can be seen from the obtained data which were almost the same as the average age of the reproductive characteristics of buffaloes in Indonesia. Gayo buffaloes in the Mesidah Sub-district experienced the first estrus (puberty) at 33.83 months old, first mating at 34.61 months old, and first calving at 45.29 months. The average gestation period was 10.51 months, the calving interval was 15.5 months, and the average service period was 3.66 months, while maintenance management was carried out extensively. Based on the results, it can be concluded that the reproductive characteristics of Gayo buffaloes in the Mesidah Sub-district, Bener Meriah Regency, still meet the standards. The average reproductive characteristics of Gayo buffaloes are generally the same as the average characteristics of other buffaloes in Indonesia.

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Tongku Nizwan Siregar

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jkh@usk.ac.id

Phone
+626517551536

Journal Mail Official
jkh@usk.ac.id

Editorial Address
Jl. Teungku Hasan Krueng Kalee No. 4, Kampus FKH Unsyiah, Kopelma Darussalam, Banda Aceh 23111, Indonesia

Location
Kab. aceh besar,

Aceh

INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name Tongku Nizwan Siregar

Contact Email jkh@usk.ac.id

Phone +626517551536

Journal Mail Official jkh@usk.ac.id

Editorial Address Jl. Teungku Hasan Krueng Kalee No. 4, Kampus FKH Unsyiah, Kopelma Darussalam, Banda Aceh 23111, Indonesia

Location Kab. aceh besar, Aceh INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
Tongku Nizwan Siregar

Contact Email
jkh@usk.ac.id

Phone
+626517551536

Journal Mail Official
jkh@usk.ac.id

Editorial Address
Jl. Teungku Hasan Krueng Kalee No. 4, Kampus FKH Unsyiah, Kopelma Darussalam, Banda Aceh 23111, Indonesia

Location
Kab. aceh besar,

Aceh

INDONESIA