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Bioprospeksi ekstrak jahe gajah sebagai anti-crd: kajian aktivitas antibakteri terhadap Mycoplasma galliseptikum dan e. coli in vitro Min Rahminiwati; Aulia Andi Mustika P.; Siti Saadah; . Andriyanto; . Soeripto; Unang P.
Jurnal Ilmu Pertanian Indonesia Vol. 15 No. 1 (2010): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

CRD is chronic respiratory disease in chicken caused by infection of Mycoplasma gallisepticum (M gallisepticum) and E. coli. Rio-prospective of jahe for controlling the disease was investigated through the study of antibacterial activity against M. gallisepticum of fresh ginger juice extract and fraction of hexan, ethyl acetate, methanol and water against M. gallisepticum and E. coli. The results showed that the juice of fresh ginger inhibited the growth of M. gallisepticum with the minimum inhibitory concentration that could inhibit the growth was 10 °/o. The fractions that effectively inhibited the growth of M. gal/isepticum are hexan fraction and water fraction with the smallest inhibition zone was found at concentration of at least 8 % and 10 % respectivelly.TLC examination results of hexan fraction showed a purple spot with Rf value of 0.9 and a dark blue spot with Rf value of 0.36. Based on Rf values and color reference, the first spot was suggested zingiberen and the second spot was gingerol. All fractions that were examined, did not show any inhibitory activity against thegrowth of E coli. Thus the extract of fresh ginger was only to be used to control the respiratory disease causedby M. gallisepticum but not coli.
Aktivitas Larvasida Biji Bengkuang sebagai Insektisida Nabati terhadap Larva Lalat Crysomya bezziana Aulia Andi Mustika; Upik Kesumawati Hadi; April Hari Wardhana; Min Rahminiwati; Ietje Wientarsih
Acta VETERINARIA Indonesiana Vol. 4 No. 2 (2016): Juli 2016
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (42.789 KB) | DOI: 10.29244/avi.4.2.68-73

Abstract

Bengkuang merupakan salah satu tanaman obat yang berpotensi sebagai bioinsektisida. Penelitian ini bertujuan mengetahui efektivitas biji bengkuang sebagai insektisida nabati terhadap larva lalat Crysomya bezziana (C. bezziana) agen penyebab miasis secara in vitro. Penelitian ini terbagi menjadi 5 kelompok perlakuan. Masing-masing sebanyak 20 Larva instar 1 (L1), Larva instar 2 (L2), dan Larva instar 3 (L3) C.bezziana digunakan untuk pengujian in vitro menggunakan pot plastik yang berisi media larva dan ekstrak ethanol biji bengkuang dengan konsentrasi bertingkat 0,06, 0,12, dan 0,25%. Coumaphos 0,06% dan akuades steril digunakan sebagai kontrol positif dan negatif. Hasil penelitian menunjukkan bahwa pada konsentrasi 0,25% mampu menyebabkan 100% kematian larva dan 100% pupa tidak menetas. Pengujian L3 menunjukkan bahwa ekstrak ethanol biji bengkuang mampu menyebabkan penurunan daya tetas pada semua konsentrasi. Pengujiaan L1 dan L2 untuk mengindikasikan efektifi tas ekstrak sebagai racun perut, sedangkan pengujian pada L3 sebagai indikasi racun kontak. Biji bengkuang memiliki daya larvasida terhadap beberapa jenis larva serangga C. bezziana.
Pengamatan Profil Darah Domba Terinfestasi Larva Chrysomya Bezziana dan Diberi Terapi Krim Herbal Sus Derthi Widhyari; Aulia Andi Mustika; Ietje Wientarsih; Lina Noviyanti Sutardi; Arief Purwo Mihardi; Esti Dhamayanti
Acta VETERINARIA Indonesiana Vol. 6 No. 2 (2018): Juli 2018
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (733.616 KB) | DOI: 10.29244/avi.6.2.8-15

Abstract

Larva Chrysomya bezzianamerupakan penyebab kejadian miasispada hewan ternak, dan merupakan masalah cukup serius karena dapat merugikan secara ekonomi.Pengobatan secara kimiawi dapat beresiko terhadap residu yang ditimbulkan, oleh karena itu perlu dicari obat alternatif berupa obat herbal yang aman bagi tubuh. Penelitian ini bertujuan untuk mengetahui status kesehatan melalui pemeriksaan gambaran darah pada domba garut yang diinfestasi larva Chrysomya bezziana dan diberi terapi krim herbal sirih merah. Semua kelompok perlakuan dilakukan infestasi larva kecuali kelompok kontrol (K0). Pembuatan luka insisi dan diinfestasi 50 larva pada setiap lubang. Penelitian ini terdiri dari 5 kelompok perlakuan yaitu kelompok kontrol (K0), kelompok dengan terapi krim sirih merah 2% (P1), kelompok dengan terapi krim sirih merah 4% (P2), kelompok dengan terapi krim asuntol (KP), dankelompok tanpa terapi (KN).Pengambilan darah dilakukan pada awal pengamatan (pre terapi) dan akhir pengamatanyaitu hari ke-7 setelah terapi diberikan (post terapi). Parameter yang diamati berupa jumlah sel darah merah, kadar hemoglobin dan nilai hematokrit.Infestasi larva Chrysomya bezzianadan pemberian krim herbal sirih merah tidak berpengaruh terhadap jumlah eritrosit, nilai hematokrit, dan kadar hemoglobin.Hasil penelitian menunjukkan pemberian krim sirih merah 4% memberikan profil darah yang paling baik. Krim sirih merah memiliki kemampuan dalam penyembuhan luka
Kombinasi Ekstrak Kunyit (Curcuma domestica val.) dan Mengkudu (Morinda citrifolia) sebagai Antidiare Lina Noviyanti Sutardi; Aulia Andi Mustika; Andriyanto; Rendi Pratama Mukti
Acta VETERINARIA Indonesiana Vol. 10 No. 1 (2022): Maret 2022
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.10.1.80-86

Abstract

Kunyit dan mengkudu sudah digunakan sebagai pengobatan tradisional penyakit. Tanaman ini secara empiris digunakan untuk pengobatan diare. Akan tetapi, sejauh ini belum ada studi farmakologi aktivitas antidiare kombinasi ekstrak kunyit dan mengkudu. Tujuan penelitian ini adalah mengevaluasi secara ilmiah pengaruh kombinasi ekstrak kunyit dan mengkudu terhadap diare yang diinduksi minyak jarak pada mencit. Penelitian ini menggunakan 25 ekor mencit yang dibagi menjadi 5 kelompok. Kelompok dibagi menjadi kelompok kontrol negatif (Tween 80 1%), kelompok kontrol positif (Loperamid HCl) dan kelompok perlakuan kombinasi ekstrak kunyit dan mengkudu (perbandingan 1:1) dosis 20 mg/kg BB, 40 mg/kg BB dan 80 mg/kg BB. Perlakuan dilakukan secara peroral. Parameter yang digunakan adalah proteksi intestinal (frekuensi defekasi mencit setiap 30 menit selama empat jam) dan transit intestinal (persentase lintasan penanda usus mencit). Hasil penelitian menunjukkan kombinasi ekstrak kunyit dan mengkudu dosis 20 mg/kg BB, 40 mg/kg BB dan 80 mg/kg BB memiliki efek antidiare dalam menurunkan frekuensi defekasi mencit dan menurunkan persentase lintasan penanda usus mencit, dengan dosis terbaik 80 mg/kg BB. Hasil penelitian ini memberikan informasi ilmiah terhadap penggunaan kombinasi ekstrak kunyit dan mengkudu untuk pengobatan diare.
Aktivitas Antibakteri Ekstrak Etanol dan Destilat Jahe dan Sirih terhadap Mycoplasma gallisepticum dan Escherichia coli (ANTIBACTERIAL ACTIVITY OF ETHANOL EXTRACT AND DESTILAT OF GINGERS AND PIPER BETLES AGAINST MYCOPLASMA GALLISEPTICUM AND ESCHERICHIA CO Min Rahminiwati; Aulia Andi Mustika; Agung Zaim; Lina Novianti Sutardi
Jurnal Veteriner Vol 16 No 4 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Betle and ginger juice extracts have been reported to have antibacterial activity against E. coliand M. gallisepticum. However, fractionation analysis showed the have differences in their antibacterialpotency which appeared to be associated with the nature of the solvent polarity. This study wasconducted to obtain information about antibacterial activity of Piper betles and gingers extract againstE. coli and M. gallisepticum. Three types of betle, P. betle, P. betle var nigra, P. crocatum, wereexamined for their antibacterial activity using disc method and three types of ginger, Zingiber officinale,Z. officinale Linn var rubrum, and Z. majus rumph which were extracted by soxhletation using ethanol30%, 60% and 90% as well as distestillation for three and five hours. Piper betle and P. crocatumconsistently have antibacterial effect against E. coli whereas Z. officinale consistently has antibacterialeffect against M. gallisepticum, either extracted by distillation or soxhletation. Piper betle is potentialyield of distillate and extract that has the highest antibacterial activity against E. coli, and M.gallisepticum with inhibitory zone were 9.76 mm and 22 mm respectively.
Daun Binahong (Andredera cordifolia Steenis) Sebagai Alternatif Insektisida Terhadap Miasis yang Disebabkan Lalat Chrysomya bezziana (ANREDERA CORDIFOLIA STEENIS (BINAHONG) LEAF AS AN ALTERNATIVE INSECTICIDE AGAINST CHRYSOMYA BEZZIANA CAUSED MYIASIS) Ietje Wientarsih; Aulia Andi Mustika; April Hari Wardhana; Dodi Darmakusumah; Lina Noviyanti Sutardi
Jurnal Veteriner Vol 18 No 1 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (103.219 KB) | DOI: 10.19087/jveteriner.2017.18.1.121

Abstract

Larvae Chrysomya bezziana caused myiasis in most livestock in Indonesia. Drugs of choice for treating myasis are synthetic insecticides. Unfortunately the insecticides has negative effect on animal product, killed insect non target and insect resistenst. The research was conducted on the activity of ethanol extract of binahong leaves against various stages of C. bezziana larvae (L1, L2, and L3). Five treatments group tested were: group without any treatment (negative control), groups treated with ethanol extracts of binahong leaves 0.5%, 1%, and 2%, respectively, positive control were given coumaphos. The treatments were applied with five replications. Number of living larvae and pupae, pupae weight and number of imago were observed. The results demonstrated that 0.5% of the extract effective consentration which was able to kill the larvae (L1 and L2). Finally 2% of the extract was the most effective concentration which was able to kill larvae (L3) and decrease the pupae weight. Ethanol extract of binahong leaves was significantly able to reduce the growth of C. bezziana larvae due to contact and digestive effect of the active compounds contained in Anredera cordifolia leaf. ABSTRAK Miasis merupakan infestasi larva lalat yang terdapat pada jaringan hidup. Penyakit ini umum menyerang hewan maupun manusia. Obat yang digunakan sebagai pengendalian kasus miasis di lapangan sampai saat ini sangat terbatas dan menggunakan insektisida sintetik. Penelitian ini bertujuan melihat aktivitas ekstrak etanol daun binahong terhadap larva lalat Chrysomya bezziana pada stadium L1, L2, dan L3. Penelitian dibagi menjadi lima kelompok perlakuan dengan lima ulangan. Kelompok perlakuan terdiri dari kontrol negatif (tanpa pemberian obat), kelompok perlakuan yang diberikan ekstrak binahong dengan konsentrasi berturut-turut 0,5%, 1%, 2%, dan kelompok yang diberikan coumaphos (kontrol positif). Peubah yang diamati adalah jumlah larva yang hidup dan berkembang menjadi pupa, bobot pupa, dan daya tetas pupa menjadi imago. Hasil penelitian menunjukkan bahwa ekstrak etanol daun binahong pada konsentrasi 0,5% mempunyai efikasi sebagai larvasidal terhadap L1 dan L2 serta pada konsentrasi 2% merupakan konsentrasi terbaik sebagai larvasidal L3 larva C. bezziana. Ekstrak etanol daun binahong memiliki kemampuan menghambat larva lalat C. bezziana akibat dari efek cerna dan efek kontak bahan aktif yang terkandung di dalam daun binahong.
Peningkatan Produktivitas Ayam Petelur Melalui Pemberian Ekstrak Etanol Daun Kemangi (INCREASED LAYING HENS PRODUCTIVITY THROUGH THE ADMINISTRATION OF ETHANOL EXTRACT OF KEMANGI LEAVES) Andriyanto .; Ridi Arif; Mohammad Miftahurrohman; Yayuk Sri Rahayu; Erli Chandra; Alifiana Fitrianingrum; Risna Anggraeni; Diah Nugrahani Pristihadi; Aulia Andi Mustika; Wasmen Manalu
Jurnal Veteriner Vol 15 No 2 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Empirically, kemangi leaves reported to increase health quality in human and livestock. Thepreliminary study was designed to explore the potency of ethanol extract of kemangi leaves to increaselaying hens performance. Sixteen laying hens (pullet) were divided into 4 groups and repeated 4 times.Control group was laying hen administered aquadest orally, treated group was laying hen administeredextract of kemangi leaves orally at a dose of 1, 2, and 3 mg/kg BW, respectively. Every day, the experimentallaying hens were fed for 3 times and drinking water was provided ad libitum. Variables observed were thenumber of eggs, egg weight, time of first laying, egg laying intervals, egg quality ( water content, crudeprotein, and crude fat), and liver function (SGPT and SGOT values) . Results of this research showed thatadministration of kemangi leaves extract at a dose of 3 mg/kg BW significantly increased the number ofegg production and egg weight (p<0.05). Time of first laying and laying interval did not show any significantdifference among treatments. Examination of moisture, crude protein, and crude fat content of the eggindicated that the administration of kemangi leaves extract did not affect egg quality. Extract of kemangileaves decreased SGPT and SGOT values that indicated improvement of liver function. It was concludedthat administration of ethanol extract of kemangi leaves could increase laying hens productivity byimprovement of liver function that is critical in vitellogenesis.
Aktivitas Antipiretik Ekstrak Etanol Buah Belimbing Wuluh (Averrhoa bilimbi) pada Tikus Putih Jantan (ANTIPYRETIC ACTIVITY OF ETHANOL EXTRACT OF BELIMBING WULUH (AVERRHOA BILIMBI) IN MALE WHITE RAT) Andriyanto Andriyanto; Ni Made Ria Isriyanthi; Edwin Ligia Sastra; Ridi Arif; Aulia Andi Mustika; Wasmen Manalu
Jurnal Veteriner Vol 18 No 4 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (106.873 KB) | DOI: 10.19087/jveteriner.2017.18.4.597

Abstract

The experiment was conducted to study the activity of ethanol extract of belimbing wuluh (EEBW) as antipyretic. Fever induction was done by using difteri pertusis tetanus (DPT) vaccine intramuscularly at a dose of 0.1 mL/100 g BW. Male white rat strain spraque dawley with range of weight by 200 to 300 g was used in this research.Fifteen experimental rats were used to explore the specific time of fever (time of early fever and time of peak fever) which were divided in 2 treatments, i.e. 10 experimental rats with DPT vaccine injection and 5 experimental rats without DPT vaccine injection as control.Fourty experimental rats were used to study effectivity of EEBW as antipyretic which were grouped with factorial randomized design with 4 x 2 and 5 replications. The first factor was various substance administered which consisted of aquadest (control), EEBW 0.88 g/kg BW, EEBW 1.75 g/kg BW, and aspirin 0.004 g/kg BW.The second factor was time of fever detection, i.e. time of early stage fever and time of peak fever. The variable was measured with rectal temperature of the experimental rats by using digital thermometer (correction factor 0.01oC) every 30 minutes. The data were analyzed with t-student dan general linear model (GLM). Increasing of rectal temperature of experimental rats with DPT injection began at 30 minutes post-DPT injection (time of early stage fever) and reached time of peak fever at 210 minutes post-DPT injection. Ethanol extract of belimbing wuluh at dose of 1.75 g/kg BW administered at early fever lowered rectal temperature of the experimental rats compared to the other groups. It was concluded that the administration of EEBW at dose of 1.75 g/kg BW was effective combination in dose and time administration as an antipyretic.
Potensi Salep Ekstrak Daun Sirih Merah Terhadap Profil Eritrogram Sebagai Terapi pada Luka Miasis (THE EFFECT OF RED BETEL LEAF EXTRACT OINTMENT IN ERYTHROGRAM PROFILES AS THERAPY IN MYASIS WOUND) Sus Derthi Widhyari; Ietje Wientarsih; Aulia Andi Mustika; April Hari Wardhana; Dodi Darmakusuma; Lina Noviyanti Sutardi; Muhammad Bintang
Jurnal Veteriner Vol 19 No 1 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (84.272 KB) | DOI: 10.19087/jveteriner.2018.19.1.30

Abstract

The objectives of this research was to examine the efficacy of red betel extract ointment to eritrogram profiles in infected sheep myiasis. Fifteen female sheeps were divided into five treatments, which were sheep without wound, treated with asuntol 2%, without any treatment,with red betel leaf extract ointment 2% and 4 %. Three incision wounds were made on the sheep’s back, 50 larvae given to every wounds to four treatment, except the healthy sheep (K). Blood was taken on the 0, 3th and 7th to determine erythrocytes counts, hematocrit values (PCV) and hemoglobin levels (Hb). The result showed that oitment of red betel leaf extract do not change the erythrogram profiles. The administration of red betel leaf extract ointment 4% has better eritrogram profiles than 2% Oitment of red betel leaf extract can be used as myasis therapy.
Pemberian Pregnant Mare Serum Gonadotropin Sebelum Perkawinan dan Jamu Selama Kebuntingan untuk Memperbaiki Performa Anak Domba (ADMINISTRATION OF PREGNANT MARE SERUM GONADOTROPIN AND JAMU DURING GESTATION TO IMPROVE FOR LAMB PERFOMANCE) Andriyanto .; Ridi Arif; Adi Winarto; Leo Sapelani Soinbala; Bondan Achmadi; Aulia Andi Mustika; Diah Nugrahani Pristihadi; Amrozi .; Wasmen Manalu
Jurnal Veteriner Vol 16 No 3 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Administration of pregnant mare serum gonadotropin (PMSG) hormone prior to mating increasesquality of lamb born on one and two litter sizes, but in three litter size the motality is higher. Administrationof traditional herbal medicine “jamu” consisting of ginger,green chiretta, cinnamon, Zingiberzerumbet, andpepper during gestation increases performance of lambs. This research was conducted to explore theeffectivity of PMSG injection prior to mating and jamu administration during gestation to increase lambsperformance. Eighteen priangan fat-tail ewes with weight around 20-25 kg were injected with PGF2á at adose of 10 mg/ewe twice with 11 days interval to synchronize estrous cycle. Injection of PMSG at a dose of200 IU/ewe was conducted at the second PGF2á injection. The experimental ewes were mated naturally anddivided into a randomized design with a 2 x 3 factorial arrangement with three replications. The firstfactor was dose of PMSG with two levels i.e., 0 and 200 IU/ewe. The second factor was dose of jamu withthree levels i.e., 0, 15, and 30 mL/ewe. Jamu was administered orally every week during gestation. Injectionof PMSG and administration of jamu decreased prenatal mortality, increased ratio of lamb per ewe, increased total lamb born with average birth weight and total birth weight were higher in jamu at a doseof 15 and 30 mL/ewe by 30.02 and 31.76%, repectively. During the first month postnatal, lambs born toewes injected by PMSG and administered jamu had higher number of lambs survive, average weight, totalweight around 3 times as compared to control. It was concluded that injection of PMSG prior to mating andadministration of jamu during pregnancy increase lamb birth weight and improve the quality of lamb.
Co-Authors . andriyanto . Soeripto Adi Winarto Afiff , Usamah Aisyah Nurfitria Ayumi Alifiana Fitrianingrum Alifin Wisnu Ananta Alpayet, Rahmat Amaq Fadholly Amperanoto, Agus Amrozi Andriyanto Andriyanto . Andriyanto . Andriyanto Andriyanto Andriyanto Andriyanto Andriyanto Andriyanto Andriyanto Andriyanto Andriyanto Andriyanto Andriyanto Andriyanto Andriyanto Andriyanto Andriyanto Andriyanto, Andriyanto Andriyanto Anggia Murni Wijiati Anggraeni, Henny Endah Anggraini, Lutvi Anisa Rahma, Anisa April Hari Wardhana April Hari Wardhana April Hari Wardhana Arief Sabdo Yuwono Aurelia, Sharon Bambang Pontjo Priosoeryanto Bondan Achmadi Budi Purwanto Diah Nugrahani Pristihadi Diky Yuliansah Diky Yuliansah Dodi Darmakusuma Dodi Darmakusumah Edwin Ligia Sastra Ekowati Handharyani Elpita Tarigan Elpita Tarigan Erli Chandra Esti Dhamayanti Fadiela Salima Putri Febrita, Joana Fedik Abdul Rantam Fifi Gus Dwiyanti Firda Agustin Firda Agustin Firda Agustin, Firda Hamdika Yendri Hamdika Yendri Hamdika Yendri Hamdika Yendri Putra Hudaya, Aang IBNUL QAYIM IETJE WIENTARSIH IPB, BPKB Irarang, Yusa Jusuf, Elena Adjani Kharisma Mardathilah Kharisma Mardathilah Khonsa Khonsa Kusdiantoro Muhamad Lailan Safina Nasution Leliana Nugrahaning Widi Leliana Nugrahaning Widi Leliana Nugrahaning Widi Leliana Widi Leo Sapelani Soinbala Lina Novianti Sutardi Lina Novianti Sutardi, Lina Novianti Lina Noviyanti Sutardi M. Agung Zaim Adzkiya Madyastuti, Rini Mardathilah, Kharisma Meilisa Lidya Margarita Meti Ekayani Michella Hoseana Wijaya Mohammad Miftahurrohman Muhammad Bintang Nababan, Ratna Kristiani Nabilah, Avida Shahnaz Ni Made Ria Isriyanthi Purohita, Adwisto Saktika Putra, Hamdika Yendri Putra, Heriansyah Putri, Fadiela Salima Rahmawati Januar Rahminiwati, Min Rai Sita Rendi Pratama Mukti Ridi Arif Rifnadhi, Bayu Rina Mardiana Rindy Fazni Nengsih Rindy Fazni Nengsih Risna Anggraeni Rukmana, Adi Safitri, Nadiya Santosa, Alfian Sari, Windi Mayang Silmy Kamila Widyanti Siti Saadah SM Leluala Subangkit, Mawar Sultania A Suleman Sus Derthi Widhyari Trioso Purnawarman Unang P. Upik Kesumawati Hadi Utami, Annisa Dwi Wasmen Manalu Widi, Leliana Nugrahaning Yayuk Sri Rahayu Yendri, Hamdika Yuliansah, Diky Yusa Irarang Zaenal Abidin