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Biosaintifika: Journal of Biology & Biology Education
Published by Universitas Negeri Semarang
ISSN : 2085191X     EISSN : 23387610     DOI : https://doi.org/10.15294/biosaintifika
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Biosaintifika: Journal of Biology & Biology Education {PISSN 2085-191X| EISSN 2338-7610} published scientific papers on the results of biology and biology education research {see Focus and Scope}. Editor accepts the article has not been published in other media with the writing format as listed on page manuscript writing guidelines {see Author Guidelines}. The journal published three times a year, on April, August & December and published by Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Semarang in collaboration with Perhimpunan Biologi Indonesia.
Arjuna Subject : Ilmu Sosial - Pendidikan
Articles 142 Documents
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Antiproliferative and Apoptosis-Inducing Effects of Iodine Molecule in Differentiated Primary Thyroid Cancer Cells: In Vitro Study Yulia Kurniawati; Jihan Atiqah; Malinda Meinapuri; Daan Khambri; Achmad Hussein Kartamihardja; Aisyah Elliyanti
Biosaintifika: Journal of Biology & Biology Education Vol. 18 No. 1 (2026): April 2026
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v18i1.42523

Abstract

Thyroid cancer poses a persistent clinical challenge, particularly when conventional therapies fall short. Growing evidence suggests that molecular iodine (I₂) may exert direct antitumor effects beyond its classical role in thyroid hormone synthesis. This study evaluated the antiproliferative and apoptosis-inducing effects of I₂ on primary cell cultures derived from differentiated thyroid cancer tissue and established dose- and time-response relationships. Primary cultures were exposed to I₂ at concentrations of 5, 10, 20, 40, 80, and 100 µM for 24, 48, and 72 hours. Cell viability was assessed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, with IC₅₀ calculated accordingly. Apoptosis was characterized by acridine orange/propidium iodide (AO/PI) double-staining, analyzed by fluorescence microscopy and ImageJ software. I₂ demonstrated clear antiproliferative and apoptotic activity in a dose- and time-dependent manner. The strongest effect appeared at 100 µM after 72 hours, reducing viability to 1.39% (p < 0.001). At the IC₅₀ of 2.09 µM, iodine significantly suppressed proliferation and induced apoptosis, with an apoptosis rate of 81.77% ± 12.24% (p = 0.004). These findings confirm the meaningful antineoplastic potential of I₂ against differentiated thyroid cancer cells. This study supports SDG 3 (Good Health and Well-Being), contributing to efforts to develop more equitable, effective cancer treatment options and to reduce cancer-related morbidity globally.
Defense Related Gene Expression and Biochemical Responses in Cymbopogon nardus Under Stresses Rofiatun Solekha; Fita Fitriatul Wahidah; Fadhilatustsani Sofialana; Aisyah Hadi Ramadani
Biosaintifika: Journal of Biology & Biology Education Vol. 18 No. 1 (2026): April 2026
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v18i1.44671

Abstract

Cymbopogon nardus employs complex molecular and biochemical mechanisms to adapt to biotic and abiotic stresses through regulation of gene expression, antioxidant activity, and ion transport. This study analyzed the expression of CnWRKY12, CnSOD, and CnHKT1 genes along with the enzymatic activities of phenylalanine ammonia-lyase (PAL) and superoxide dismutase (SOD). Methods used Quantitative PCR and biochemical assays and enzymatic responses with HPLC. Results showed that CnWRKY12 was strongly upregulated under drought (5.75-fold) and fungal infection (5.05-fold), confirming its role as a transcription factor regulating the phenylpropanoid defense pathway. The SOD gene exhibited the highest induction under drought (3.0-fold). Meanwhile, CnHKT1 expression has been highly induced under salinity (7.31-fold) but has been repressed under drought and fungal stress, thereby indicating its role in maintaining Na⁺/K⁺ ionic homeostasis. Enzymatic assays to have supported: PAL activity to have been highest during fungal infection (1.03 unit mg⁻¹ protein), whereas SOD activity to have peaked under drought conditions (0.99 unit mg⁻¹ protein). The correlation between gene expression and enzyme activity has demonstrated an integrated defense mechanism in C. nardus, in which CnWRKY12-PAL interactions have strengthened biotic resistance and CnHKT1 has regulated ionic balance under salinity. These conclusions into stresspecific defense responses have contributed to a broader understanding of tolerance mechanisms in aromatic and medicinal grasses. This research to growth SDG’s Good Health and Well being because this research has the potential to increase the production of bioactive compounds from plants that are beneficial for human health.

Page 15 of 15 | Total Record : 142

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