cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
-
Contact Email
biosaintifika@mail.unnes.ac.id
Phone
-
Journal Mail Official
biosaintifika@mail.unnes.ac.id
Editorial Address
D6 Building Floor 1 Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Semarang, Sekaran, Gunungpati, Semarang, Central Java, Indonesia 50229
Location
Kota semarang,
Jawa tengah
INDONESIA
Biosaintifika: Journal of Biology & Biology Education
Published by Universitas Negeri Semarang
ISSN : 2085191X     EISSN : 23387610     DOI : https://doi.org/10.15294/biosaintifika
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Biosaintifika: Journal of Biology & Biology Education {PISSN 2085-191X| EISSN 2338-7610} published scientific papers on the results of biology and biology education research {see Focus and Scope}. Editor accepts the article has not been published in other media with the writing format as listed on page manuscript writing guidelines {see Author Guidelines}. The journal published three times a year, on April, August & December and published by Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Semarang in collaboration with Perhimpunan Biologi Indonesia.
Arjuna Subject : Ilmu Sosial - Pendidikan
Articles 107 Documents
 7   8   9   10   11 
Molecular Characteristics of Lopang (Gymnopetalum cochinchinense) Originating from Riau Based on matK and trnL-trnF Intergenic Spacer Herman, Herman; Akmal, Fidia; Nurbaiti, Nurbaiti; Siahaan, Citra Winarni; Lestari, Wahyu; Adiwirman, Adiwirman; Altuhaish, Adeel Abdulkarim Fadhl; Roslim, Dewi Indriyani
Biosaintifika: Journal of Biology & Biology Education Vol. 17 No. 3 (2025): November 2025
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v17i3.24522

Abstract

Lopang (Gymnopetalum cochinchinense) is a herbaceous plant traditionally consumed as a vegetable by communities in Riau Province, Indonesia. However, DNA barcode sequence data for this species remain limited. These findings underscore the importance of analyzing DNA barcode sequences of matK and trnL-trnF intergenic spacer (IGS) lopang from Riau. This study highlights the analysis of DNA barcode sequences matK and trnl-trnf intergenic spacer (IGS) in lopang from Riau province. This study applies sampling, DNA extraction, Polymerase Chain Reaction (PCR), electrophoresis, sequencing, and bioinformatics data analysis using BioEdit 7, BLASTn (Basic Local Alignment Search Tool) to find sequence similarities with the GenBank database, Management and analyzed DNA sequences with MESQUITE, Multiple alignments using ClustalW and MEGA11 to create dendrograms. Fresh leaves were collected from Rokan Hulu Regency in Riau Province. The results showed that matK and trnL-trnF IGS DNA sequences of lopang measured 752 bp and 410 bp, respectively. BLASTn analysis revealed that lopang has 99.73% similarity with G. chinense based on the matK sequence and 99.76% similarity based on the trnL-trnF IGS sequence. The analysis revealed a variation of 13 nucleotides, 1 critical nucleotide and no indels in the matK sequence, while in the trnL-trnF IGS sequence there were 19 nucleotide variations, 1 critical nucleotides and 5 indels. As a final point, lopang from Riau is closely related to G. chinense. The findings of this research contribute to the molecular identification of this species and benefit science, such as providing an understanding of plant evolution, species identification, genetic analysis, and the development of molecular markers in the Cucurbitaceae family.
Therapeutic Potential of Ethanol Extract from Durian Peel on Testes Microanatomy in Diabetic Male Rats Syakera, Cinta Etna; Isdadiyanto, Sri; Sunarno , Sunarno; Ermanjaya, Laode
Biosaintifika: Journal of Biology & Biology Education Vol. 17 No. 3 (2025): November 2025
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v17i3.25448

Abstract

Diabetes mellitus (DM) is a degenerative disease that can lead to complications such as testicular atrophy. As DM requires lifelong treatment, natural and safe therapeutic alternatives are needed. Durian peel, as a natural material, has potential. This study investigated the effects of ethanol extract of durian peel (EEDP) on testicular histology in diabetic rats. A total of 25 male Sprague-Dawley rats, aged 2 months and weight ±200 g, were randomly assigned to five groups: healthy (K0), diabetic (K1), and diabetic+EEDP at doses of 500 (K2), 750 (K3), and 1,000 mg/kgBW (K4), given orally for 28 days. After treatment, the rats were euthanized, and their testes were removed, weighed, and examined using hematoxylin-eosin staining. Observed variables included testis weight, seminiferous tubule diameter, epithelial thickness, and spermatogenesis stages. Statistical analysis ANOVA (α=0,05) showed significant improvements (p < 0,05) in the EEDP-treated groups. It is concluded that EEDP at doses of 500-1,000 mg/kgBW improved testicular structure in diabetic rats.. The novelty of this study lies in the utilization of durian peel ethanol extract at varying doses of 500–1,000 mg/kgBW as a natural therapy to improve testicular histological damage caused by diabetes. This study serves as a basis for the development of herbal antidiabetic drugs with protective effects on the reproductive system. The findings are expected to contribute to biomedical science and provide opportunities for utilizing durian peel waste as a value-added material for public health.
Heterotrigona itama Pollen Analysis as a Bioindicator of Floral Diversity in South Kalimantan Oil Palm Agroforestry Kadarsah, Anang; Ulimaz, Almira; Yardani, Jesi; Satriadi, Trisnu; Ogbodo, John Agbo
Biosaintifika: Journal of Biology & Biology Education Vol. 17 No. 3 (2025): November 2025
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v17i3.34142

Abstract

Background: The expansion of oil palm plantations in Indonesia drives deforestation and biodiversity loss, threatening pollinator communities. Agroforestry systems within oil palm landscapes may enhance floral resources, but their effectiveness is poorly understood. This study evaluates the potential of stingless bee (Heterotrigona itama) pollen analysis as a tool for monitoring floral diversity in different oil palm agroforestry systems. Methods: Pollen samples were collected from H. itama colonies in five distinct oil palm agroforestry systems (block-type, intercropping, row-type, fence-type, and palm oil-livestock) across South Kalimantan. Pollen types were identified microscopically, and diversity metrics (Shannon Index, Simpson Index, and Evenness) were calculated to assess foraging patterns and floral resource availability. Results: Analysis identified 30 plant species from 22 families. Agroforestry type significantly influenced pollen composition. Block-type systems exhibited the highest species richness (14 types), while fence-type systems showed the lowest (9 types) with heavy dominance by maize (Zea mays, 41.4%). Intercropping systems were dominated by Acacia mangium (51.2%). Row-type systems demonstrated the most balanced foraging (highest evenness). A moderate Sørensen similarity index (0.63) between pollen and field vegetation confirmed bees as effective samplers of landscape floral diversity. This research confirms that H. itama pollen analysis is an efficient bioindicator tool, revealing that agroforestry management directly shapes pollinator resource availability. Complex systems (e.g., block-type, row-type) support higher floral diversity, which is crucial for pollinator conservation and sustainable productivity in oil palm-dominated landscapes. This method provides a practical approach for guiding biodiversity-inclusive agricultural practices.
Mosaic of Drosophila Behavior Due to Bisphenol-A Exposure: From Hyperactivity to Aversion Fauzi, Ahmad; Ramadhan, Muhamad Justitia; Kharomah, Sindi; Kharomah, Sinta; Zahrah, Natasya Adiba; Setiawan, Deny; Buroidah, Hikmah; Bilad, Muhammad Roil; Zubaidah, Siti
Biosaintifika: Journal of Biology & Biology Education Vol. 17 No. 3 (2025): November 2025
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v17i3.34229

Abstract

Bisphenol-A (BPA) is classified as an endocrine-disrupting chemical that is widely found in everyday food. However, various studies have reported inconsistent results regarding the effects of this exposure on behavior. This study aimed to evaluate how dose, genetic background, and duration of exposure interact to shape behavioral responses in Drosophila melanogaster exposed to BPA. Three strains (Wild-type, white-eye, and black-body) were cultured on a medium containing 0, 0.25, and 0.5 mg/mL BPA for two generations. Before exposure (G0), after the first generation of exposure (G1), and after the second generation of exposure (G2), three behaviors were measured: larval crawling speed, number of larval contractions, and larval exploration distance. Repeated-measures ANOVA revealed a non-monotonic pattern in behavioral responses. Each behavior exhibited distinct sensitivities to treatment factors, and complex interactions were observed between genetic factors, dose, and time (generation). Furthermore, the effects of BPA are not general but rather specific to the type of behavior observed. Generational effects are evident in some behavioral data. In addition, fly strain plays a role in determining the effect of exposure on behavioral responses.
The Environmental Factors Enhance the Growth and Survival of Rhizophora Seedlings Sinaga, Na Dame Maulibulung; Pradipta, Aruna; Martuti, Nana Kariada Tri; Jabar, Muhammad Abdul; Soleha, Iro Datus
Biosaintifika: Journal of Biology & Biology Education Vol. 17 No. 3 (2025): November 2025
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v17i3.35777

Abstract

Coastal ecosystems are synonymous with mangrove trees. Threatened mangrove existence is tantamount to reduced biological existence, weakened coastal protection, and reduced carbon storage. Moreover, mangrove growth naturally depends on the quality of seedlings supported by proper nursery management. This study aims to evaluate the impact of four environmental factors (light intensity, salinity, pH, and tidal frequency) on the growth and survival of Rhizophora: Rhizophora mucronata, Rhizophora stylosa, and Rhizophora apiculata cultivated in nurseries with a water system integrated with the Internet of Things (IoT). A single-factor design was applied, with each treatment bed containing 600 seedlings (200 per species) and an uncontrolled bed as a control. Weekly random sampling of 20% of seedlings from each treatment for eight weeks. Data were analyzed using a two-way ANOVA with treatment and species as fixed factors, followed by the Tukey HSD test at a significance level of 5%. The results showed that R. mucronata in the light treatment (without shade net) achieved the highest survival rate (98.8%) and the largest stem diameter, which was 0.698 cm. In addition, the highest seedling height was R. mucronata in the high-yield salinity treatment, reaching 56.78 cm, and the highest number of leaves was recorded in R. apiculata in the salinity treatment, with an average of 6.92 leaves per seedling. IoT-integrated nurseries stabilize water supply and tidal cycles, reduce mortality to below 10%, and enhance seedling quality. These findings indicate that nursery environmental management can improve the consistency of mangrove seedling growth and support large-scale restoration.
Characterization and Evaluation of Tobacco Stem Waste Liquid Smoke as an Eco-Friendly Tofu Bio-preservation Purwantisari, Susiana; Rasyid, Rido Baharudin Ar; Wardana, Tegar Pratama Kayong; Arfieta, Frisca; Wibisono, Shafa’ Khairunnisa; Jaya, Myra Ivana Yulia; Dafip, Muchamad
Biosaintifika: Journal of Biology & Biology Education Vol. 17 No. 3 (2025): November 2025
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v17i3.21552

Abstract

Tofu preservation often uses unsafe chemicals, especially formaldehyde, that trigger health problems in humans. Therefore, it is necessary to develop preservation methods that are both effective and safe for human health. Liquid smoke derived from tobacco stem waste is well known to contain antioxidant and antimicrobial properties that may be suitable for use as a preservative compound for tofu. This study aims to evaluated the potential of tobacco stem liquid smoke as a natural preservative by analyzing its chemical profile, antimicrobial efficacy, and impact on tofu characteristics. Tofu samples were soaked in 0%, 5%, and 15% liquid smoke solutions and stored at room temperature for 24 hours. GC-MS analysis confirmed the presence of key compounds: phenolics (51.21%), fatty acids (30.82%), and ketones (17.96%). The treatment significantly improved preservation metrics, reducing the microbial load by up to 81% and lowering the pH to 5.1. The moisture content was reduced while protein levels were proportionally maintained. The 15% concentration provided the highest antimicrobial effect, enhanced aroma, and imparted a distinctive smoky flavor, yet the treatment was preferred by panelists for its better preservation of original color and soft texture. These findings highlight the potential of tobacco stem liquid smoke as a natural, low-cost preservative for tofu, promoting the valorization of biomass waste into value-added products.
Morpho-Physiological, ISSR-Based Molecular Characterization of Gamma-Irradiated Soybean Under Waterlogging Stress Solihah, Anisatus; Indahsari, Dianita; Mahendra, Belva Aria; Fadilah, Aulia Sunan; Muslihatin, Wirdhatul; Chokwiwatkul, Ratchata; Saputro, Triono Bagus
Biosaintifika: Journal of Biology & Biology Education Vol. 17 No. 3 (2025): November 2025
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v17i3.31511

Abstract

Soybeans (Glycine max) are Indonesia's third-most important food crop after rice and maize. However, because of its susceptibility to waterlogging, its productivity decreases. Gamma-ray irradiation has been used to create soybean varieties that are resistant to waterlogging. This study investigates the genetic variation and morpho-physiological traits of irradiated Grobogan soybean varieties under waterlogging stress. Soybean seeds were exposed to gamma-ray doses of 0 Gy, 25 Gy, 50 Gy, 75 Gy, and 100 Gy, then the soybean plants were submerged in water at concentrations of 0%, 100%, 150%, 200%, and 250%. The study's morpho-physiological parameters include seed weight, pod count, plant height, chlorophyll content, and seed viability. The results imply that varying waterlogging levels and radiation dosages show varied responses. All irradiation doses showed optimal growth in the control treatment, whereas 75 Gy and 100 Gy under 250% waterlogging conditions showed the most severe growth inhibition. To evaluate genetic variation among the irradiated soybean variants, five ISSR markers were employed. Because of its high PIC value of 0.391, marker ISSR-1 was found to be the most effective in detecting polymorphisms. ISSR-1 is a promising marker for assessing genetic diversity in irradiated soybean plants, according to the results. These results are valuable for the development of soybean breeding strategies targeting improved tolerance to waterlogging stress.

Page 11 of 11 | Total Record : 107

 7   8   9   10   11 

Filter by Year

2024 2025


Reset
Filter By Issues
All Issue Vol. 17 No. 3 (2025): November 2025 Vol. 17 No. 2 (2025): August 2025 Vol. 17 No. 1 (2025): April 2025 Vol. 16 No. 3 (2024): December 2024 Vol. 16 No. 2 (2024): August 2024 Vol. 16 No. 1 (2024): April 2024