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Bioeksperimen
Published by Universitas Muhammadiyah Surakarta
ISSN : 24601365     EISSN : 25272799     DOI : -
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology
Aim Bioeksperimen: Jurnal Penelitian Biologi is a peer-reviewed, open-access scientific journal dedicated to advancing knowledge across a broad spectrum of biological sciences through the publication of high-quality original research articles. The journal promotes rigorous experimental and exploratory research that advances fundamental and applied understanding in biological sciences. Bioeksperimen provides an international platform for researchers, academics, and practitioners to disseminate innovative empirical findings and scientifically robust investigations that contribute to the development of biological sciences at regional and global levels. The journal welcomes manuscripts that: 1. Present original experimental or exploratory research in biological sciences 2. Demonstrate strong methodological design and scientific rigor 3. Contribute novel data, findings, or biological insights 4. Address contemporary biological challenges and emerging scientific issues 5. Integrate laboratory, field, and computational approaches 6. Contribute meaningfully to the advancement of biological sciences All manuscripts must be original, unpublished, written in English, and must clearly demonstrate methodological soundness, data-driven analysis, and a significant contribution to biological sciences. Since March 2026, Bioeksperimen does not publish literature review articles, conceptual papers, or purely theoretical manuscripts. Focus The primary focus of Bioeksperimen: Jurnal Penelitian Biologi is on empirical biological research based on experimental, observational, or exploratory approaches. The journal prioritizes studies that: 1. Apply molecular, physiological, ecological, microbiological, or genetic techniques 2. Utilize omics-based and bioinformatics approaches to generate primary data 3. Conduct laboratory experiments, field investigations, or integrative biological exploration 4. Produce reproducible and evidence-based scientific findings Scope Bioeksperimen: Jurnal Penelitian Biologi publishes original research in the following areas: Main Topics: 1. Ecology and Environmental Sciences 2. Molecular Biology of Plants, Animals, and Microorganisms 3. Bioinformatics and Computational Biology 4. Omics-Based Studies (Genomics, Transcriptomics, Metagenomics, Proteomics, Metabolomics) 5. Basic and Applied Microbiology 6. Plant and Animal Physiology 7. Animal Behavior 8. Plant and Animal Systematics and Taxonomy 9. Genetics of Plants, Animals, and Microorganisms 10. Human Physiology
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 7 Documents
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Search results for , issue "Vol. 11 No. 2 (2025): 2025" : 7 Documents clear
Impact of Meat Processing on DNA Quantity, Purity, and Integrity in Commercial Pork Products Assessed by TaqMan qPCR Ernawati Puji Rahayu; Abinawanto
Bioeksperimen: Jurnal Penelitian Biologi Vol. 11 No. 2 (2025): 2025
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v11i2.11031

Abstract

TaqMan qPCR analysis for detecting adulteration in processed meat products requires DNA with a high quantity, purity, and integrity. This study aims to evaluate the quantity, purity, and DNA integrity of various commercial pork products focusing on their ability to amplify the Cyt b gene. Fresh pork was used as a positive control, while five processed products - jerky, floss, meatball, canned corned, and smoked pork - were extracted for analysis. The DNA concentration and purity then measured, visualized using gel electrophoresis, and subjected to qPCR amplification. Statistical analysis of DNA concentration, purity, and ct value was carried out using one-way ANOVA followed by the Tukey post hoc test. The results showed significant differences in concentration, purity, and Ct values between all samples and positive controls (P˂0.05). Meanwhile, the Tukey test showed that all samples and fresh pork concentration differed significantly (P˃0.05) except between canned corned and fresh pork (P˂0.05). The purity was significantly different between the positive controls with pork meatball and smoked pork (P˂0.05), while the Ct​ value of all samples and positive controls were significantly different (P˂0.05) except for fresh pork and jerky (P˃0.05). All the commercially processed products experienced DNA fragmentation. Meat processing affects DNA concentration, purity, genomic integrity, and the amplification ability of qPCR.
Isolation, Characterization, and Identification of Biosurfactant-Production Bacteria from Used Cooking Oil Waste Fisky Aditya Pratama; Pramesti Dewi; Ibnul Mubarok; Dewi Mustikaningtyas
Bioeksperimen: Jurnal Penelitian Biologi Vol. 11 No. 2 (2025): 2025
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v11i2.11115

Abstract

Waste cooking oil constitutes a significant environmental concern in Indonesia. It has the potential to be utilized as a substrate for biosurfactant-producing bacteria. Biosurfactants are amphiphilic compounds produced by microorganisms, containing both hydrophilic and hydrophobic components that enable them to dissolve in both water and fats, as well as to reduce surface tension. This study aims to isolate, characterize, and identify biosurfactant-producing bacteria from waste cooking oil. Isolation proccess was conducted using Mineral Salt Medium enriched with cooking oil as an inducer and Carboxy Methyl Cellulose as a carbon source and emulsifier. Two isolates were successfully obtained and purified. Their biosurfactant-producing ability was evaluated through drop collapse test and oil spreading test. One isolate demonstrated superior performance, showing positive activity in both tests, including a 0.7 cm oil spreading zone. Identification involved macroscopic observation, Gram staining, endospore staining, and a catalase test. The isolate exhibited characteristics of a Gram-positive, rod-shaped, spore-forming, catalase-positive bacterium with small, circular, milky-white colonies and a smooth surface. This identification is non-molecular and based on a phenotypic approach according to Bergey’s Manual for preliminary bacterial classification. Based on these features, the isolate is presumed to belong to the genus Bacillus, known for its robust biosurfactant production. The findings suggest that waste cooking oil can be a promising source of indigenous biosurfactant-producing bacteria, contributing to both microbial conservation and potential development of eco-friendly biotechnological products.
Alfalfa (Medicago sativa L.) as a Promising Herbal Galactagogue for Dairy Cows: A Review Ahmad Nasihin
Bioeksperimen: Jurnal Penelitian Biologi Vol. 11 No. 2 (2025): 2025
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v11i2.11779

Abstract

Milk production in dairy cows can be enhanced through herbal-based approaches like alfalfa (Medicago sativa L.), which is known for its richness in nutrients and bioactive compounds with the potential to act as an herbal galactagogue. This study reviews scientific publications from 2015 to 2025 in the Google Scholar database to explore the use of alfalfa and its benefits for dairy cow performance. The results indicate that alfalfa can increase milk production and quality, particularly in fat, protein, and lactose content. Phytoestrogen compounds such as isoflavones and coumestrol in alfalfa stimulate prolactin hormone production and promote udder tissue growth. Phenolic compounds help improve milk yield and quality, while alkaloids enhance the milk ejection reflex. Additionally, saponins serve as defaunation agents in the rumen, increase nitrogen utilization efficiency, and support metabolic balance, positively impacting overall cow health. However, high doses of alfalfa may lead to reproductive issues due to excessive estrogenic activity, such as anestrus and infertility. The effectiveness of alfalfa also depends on factors like material quality, dosage, the cow's physiological condition, and management practices. Therefore, alfalfa should be used carefully and in balanced amounts as a functional feed to maximize benefits and prevent physiological side effects in dairy cows.
Ethanolic Extract of Tamarindus indica Leaves Lowers Total Cholesterol, Triglycerides, and HDL but Without Affecting LDL in Hyperlipidemic Rats Tri Susiloningsih; Wulan Christijanti; Aditya Marianti; Nugrahaningsih WH
Bioeksperimen: Jurnal Penelitian Biologi Vol. 11 No. 2 (2025): 2025
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v11i2.12103

Abstract

Hyperlipidaemia is a condition of increased lipid levels, especially cholesterol, triglycerides, LDL and an abnormal decrease in HDL in the blood. Tamarind leaves contain flavonoid and quercetin compounds that have the ability as antihyperlipidemia agents. The purpose of this study was to examine the effect of tamarind leaf ethanol extract on the lipid profile of hyperlipidaemia rats. Samples of 16 male Wistar rats were divided into 4 groups; Control group and the tamarind leaf ethanol extract treatment group was divided into 3, namely P1 350 mg/kgBB, P2 500 mg/kgBB, and P3 650 mg/kgBB. All rats were given lard 3 mL/head/day for 2 weeks. In weeks 3 and 4, rats were given extract of Tamarindus indica leaves and 2 hours interval was given lard. The data obtained were statistically analysed using one-way ANOVA and the Least Significant Difference (LSD) test at the 95% confidence level. In this study, HDL levels decreased along with reductions in total cholesterol and triglycerides, but had no effect in reducing LDL levels. Reduced cholesterol synthesis limits substrate availability for HDL formation, resulting in proportional decreases in HDL-C. The conclusion of this study is that tamarind leaf ethanol extract has an effect in reducing lipid profile levels.
Diversity of Pollinator Insects in Anagro Garden, Bengkulu City, Indonesia Lilis Wisti; Mega Sari Apriiarti; Mariana Ade Cahaya
Bioeksperimen: Jurnal Penelitian Biologi Vol. 11 No. 2 (2025): 2025
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v11i2.12305

Abstract

Pollinator insects are considered beneficial organisms due to their essential role in plant pollination, supporting both ecological stability and agricultural productivity. However, land-use change and pesticide application may reduce pollinator diversity, making it necessary to identify existing species and assess their diversity as indicators of ecosystem health. This study aimed to identify the species and assess the diversity of pollinator insects in Anagro Garden, Bengkulu City. Field research was conducted from January to March 2025 using an exploratory survey method combined with direct observation. Sampling was carried out across multiple points within the garden. The results showed that pollinator insects observed in this area belonged to four insect orders. The calculated Shannon-Wiener Diversity Index (H′) was 1.317, indicating a moderate level of species diversity. The Dominance Index (D) was 0.283, showing that no single order overwhelmingly dominated the assemblage. The Evenness Index (E) was 0.950, reflecting a high level of evenness and very uniform distribution of individuals among species. These findings suggest that although species abundances vary, the pollinator community remains balanced. The moderate diversity level indicates that the ecosystem quality in Anagro Garden is still fairly goodHowever, the study underscores the need for ongoing monitoring and habitat management to enhance pollinator diversity and maintain ecological resilience, particularly in semi-urban agroecosystems like those found in Bengkulu City.
The Enhancing Single Cell Protein Production of Saccharomyces cerevisiae Using Soybean Hull Waste with Dextrose Variations Musa'adah .; Adisty Virakawugi Darniwa; Dede Kartika Sari
Bioeksperimen: Jurnal Penelitian Biologi Vol. 11 No. 2 (2025): 2025
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v11i2.12356

Abstract

Single Cell Protein (SCP) is a microprotein derived from the dry biomass of non-pathogenic microorganisms and offers a promising alternative protein source for both humans and animals. One of the advantages of SCP production is its potential to utilize organic waste as a growth medium, thereby contributing to environmental sustainability. Soybean hull waste has been identified as a nutrient-rich substrate capable of supporting the growth of various microorganisms used in SCP production. This study aimed to optimize the growth medium for S. cerevisiae by utilizing soybean hull waste supplemented with varying concentrations of dextrose, in order to determine the most effective dextrose level for SCP production. The experiment involved cultivating S. cerevisiae in filtrates of soybean hull waste supplemented with five different dextrose concentrations: 0% (FD0), 1% (FD1), 2% (FD2), 3% (FD3), and 4% (FD4), as well as a standard Yeast Potato Dextrose (YPD) medium used as a control. Fermentation was initiated with an inoculum concentration of 1.3×10⁶ CFU/mL across all treatments then incubated at 28–30°C for 72 hours. Biomass, moisture content and protein content of yield were measured at the end of the fermentation. The results showed the dextrose conc. significantly affected pH levels, biomass yield, moisture, and protein content of the final product. Higher dextrose conc. generally led to better yield. Among the treatments, FD4 (4% dextrose) produced the highest biomass (0.091 g/10 mL) and protein content (18.43%). These finding showed that soybean hull waste can serve as a sustainable growth medium for the S. cerevisiae-SCP production, with 4% dextrose supplementation yielding the most optimal results.
Sumberejo Community's Perspective on Non-Timber Forest Products to Support Sustainable Environment Cynthia Permata Sari; Supriyadi; Purwanto; Retno Rosariastuti; Malihatun Nufus
Bioeksperimen: Jurnal Penelitian Biologi Vol. 11 No. 2 (2025): 2025
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v11i2.13643

Abstract

This study investigates how Non-Timber Forest Resources (NTFPs) contribute to community livelihoods and environmental sustainability. Through a combination of in-depth interviews and participatory observation, this study highlights the traditional knowledge and practices of the Sumberejo community in managing these resources. This research uses a qualitative approach that combines ethnobotanical surveys, in-depth interviews, and participatory observation with 50 participants, as well as data analysis using Participatory Rapid Appraisal (PRA). The results show that the majority of respondents perceive the potential of NFTPs in the area as very important (56.25% of village officials) and important (45.83% of villagers). Respondents also consider the natural geophysical potential for NFTPs in the area to be very important (68.75% of village officials) and important (50% of villagers). Furthermore, respondents also considered the natural biophysical potential for the NFTPs area to be very important (56.25% of village officials) and important (50% of villagers). The study underscores the potential for integrating NTFP management into broader conservation strategies to promote environmental stewardship and sustainable development. The community's commitment to balancing economic needs with environmental health offers valuable insights for policymakers and conservationists aiming to implement community-based forest management models. This research contributes to a growing body of knowledge on the importance of NTFPs in sustainable environmental governance, showcasing the Sumberejo community's role as stewards of their local ecosystems while maintaining cultural heritage and securing economic well being.

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