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JOURNAL OF BIOMEDICAL SCINECES AND HEALTH
Published by Universitas Karya Husada Semarang
ISSN : 30477182     EISSN : 30626854     DOI : https://doi.org/10.34310/
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Public Health
SCOPE 1. Biochemistry (medical) This scope includes biochemical mechanisms behind the pathogenesis, diagnosis, and management of human diseases and research concerning the subjects of Medical Biochemistry. 2. Medical Sciences and Pharmacology : This scope includes applications in the health sector (principles, procedures, care, treatment) carried out and developed by health workers. 3. Traditional Herb This scope focus on the cultivation and extraction of raw materials, or the analysis of these materials’ phytochemical or pharmacological effects and toxicity, as well as their potential applications of natural extracts, fractions, or compounds in the formulation of traditional medicine. 4. Biology and Life Science Scientific articles in the scope of biology including: botany, zoology, microbiology, genetics, physiology and conservation. 5. Genetics and Molecular Biology This scope is exploration and conservation of genetic diversity, analysis of gene and protein function, genetic engineering, synthetic biology, multimics (genomics). , transcriptomics, nutrigenomics, etc.) and supported by bioinformatics and big data analysis. 6. Immunology 7. Anatomy and Physiology 8. Tropical infection and parasitology 9. Clinic health sciences.
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Penuaan
Articles 22 Documents
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DETEKSI Gamma-Aminobutiryc Acid (GABA) PADA ISOLAT BAKTERI ASAM LAKTAT PRODUK FERMENTASI CHAO PANGKEP Zaenab, Ririn Irfayanti; Ade Irma; miladiarsi
Journal of Biomedical Sciences and Health Vol. 2 No. 2 (2025): Volume 2 Nomor 2 Agustus 2025
Publisher : Universitas Karya Husada Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34310/p74k4w72

Abstract

Bakteri Asam Laktat (BAL) adalah kelompok mikroorganisme General Recognized as Safe (GRAS) yang berperan penting dalam menghasilkan senyawa fungsional, termasuk Gamma-aminobutyric acid (GABA). GABA merupakan asam amino non-protein yang memiliki fungsi sebagai penghambat neurotransmitter utama dalam sistem saraf pusat juga berperan sebagai antioksidan. Penelitian ini bertujuan untuk mengetahui kemampuan BAL dari produk fermentasi Chao khas daerah Pangkep dalam menghasilkan senyawa GABA sebagai antioksidan menggunakan metode Thin Layer Chromatography (TLC). Berdasarkan penelitian yang dilakukan diperoleh nilai Retention factor (Rf) isolat BAL C2 tanpa MSG yaitu 0,82, C2 MSG 1% 0,84, C10 tanpa MSG 0,8 dan 0,64, C10 MSG 1% 0,86 dan 0,68. Dengan aktivitas antioksidan berdasarkan nilai IC50 C2 tanpa MSG 940,11 ppm, C2 MSG 1% 1124,97 ppm, C10 tanpa MSG 884,73 ppm, dan C10 MSG 1% 939,17 ppm. Nilai Rf isolat C2 dan C10 dengan penambahan MSG 1% lebih menghampiri nilai Rf larutan standar yaitu 0,88 dan aktivitas antioksidan isolat C2 dan C10 tergolong sangat lemah dikarenakan nilai IC50 nya >200 ppm. Ini dapat menjadi dasar optimalisasi produksi GABA oleh BAL dari produk fermentasi lokal seperti Chao untuk pengembangan probiotik atau suplemen fungsional yang bermanfaat untuk kesehatan. Kata kunci: Chao, Bakteri Asam Laktat, Gamma-Aminobutyric Acid, Antioksidan Lactic Acid Bacteria (LAB) are a group of microorganisms generally recognized as safe (GRAS) that play a vital role in producing functional compounds, including Gamma-aminobutyric acid (GABA). GABA is a non-protein amino acid that functions as a major inhibitory neurotransmitter in the central nervous system and also acts as an antioxidant. This study aimed to determine the ability of LAB from a local fermented product, Pangkep Chao, to produce GABA as an antioxidant using Thin Layer Chromatography (TLC). Based on the research, the retention factor (Rf) values for LAB isolates were as follows: C2 without MSG was 0.82, C2 with 1% MSG was 0.84, C10 without MSG was 0.8 and 0.64, and C10 with 1% MSG was 0.86 and 0.68. The antioxidant activity, based on the IC50 value, was 940.11 ppm for C2 without MSG, 1124.97 ppm for C2 with 1% MSG, 884.73 ppm for C10 without MSG, and 939.17 ppm for C10 with 1% MSG. The Rf values of the C2 and C10 isolates with 1% MSG were closer to the standard solution's Rf value of 0.88, but the antioxidant activity of the C2 and C10 isolates was considered very weak because their IC50 values >200 ppm. This research can serve as a basis for optimizing GABA production by LAB from local fermented products like Chao for the development of beneficial probiotics or functional supplements
UJI BIOAKTIVITAS EKSTRAK TULANG IKAN CAKALANG (KATSUWONUS PELAMIS) TERHADAP BAKTERI STAPHYLOCOCCUS AUREUS PADA LUKA BAKAR TIKUS PUTIH (RATTUS NORVEGICUS) arista, arista; miladiarsi; Wahdaniar
Journal of Biomedical Sciences and Health Vol. 2 No. 2 (2025): Volume 2 Nomor 2 Agustus 2025
Publisher : Universitas Karya Husada Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34310/wrsgr653

Abstract

Luka bakar adalah cedera yang rentan terhadap infeksi bakteri, terutama oleh Staphylococcus aureus, yang dapat memperparah kondisi dan memperlambat penyembuhan. Bakteri ini juga mampu membentuk biofilm, membuatnya lebih sulit diobati dengan antibiotik konvensional. Penelitian ini berfokus pada aktivitas antibakteri ekstrak tulang ikan cakalang terhadap Staphylococcus aureus pada luka bakar. Penelitian ini menggunakan metode eksperimental kuantitatif. Hasil pengukuran zona hambat diketahui bahwa ekstrak tulang ikan cakalang bersifat bakteriostatistik terhadap bakteri S. aureus menggunakan ekstrak tulang ikan cakalang konsentrasi 20%, 40%, 60%,80% dan kontrol positif ciprofloxacin serta kontrol negatif etanol dan aquades steril masing- masing sebanyak 3 kali replikasi yang menunjukkan kelompok perlakuan ektrak 20%, 40%, 60%, dan 80% tulang ikan cakalang berpengaruh dalam menghambat pertumbuhan Staphylococcus aureus berdasarkan zona hambat yang terbentuk sebesar 5,4 mm, pengukuran ditunjukkan bahwa ekstrak 80% menghasilakan nilai daya hambat yang kuat paling berpengaruh dalam pertumbuhan Staphylococcus aureus.Ekstrak tulang ikan cakalang (Katsuwonus pelamis) konsentrasi 20%, 40%,60%, dan 80% dapat menghambat bakteri Staphylococcus aureus. Ekstrak konsentrasi terbesar 80% di golongkan memiliki daya hambat kuat terhadap bakteri uji. Pada penelitian ini ekstrak tulang ikan cakalang efektif dalam menghambat pertumbuhan bakteri Staphylococcus aureus.

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