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Bionature
Published by Universitas Negeri Makassar
ISSN : 14114720     EISSN : 26545160     DOI : 10.35580
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology
Bionature is a journal that contains writings based on the results of research, conceptual ideas, studies, and the implementation of the theory in the field of biology.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ecology, Evolution, Behavior and Systematics
Articles 5 Documents
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Search results for , issue "Vol. 26 No. 1 (2025): April-Juni 2025" : 5 Documents clear
Antibacterial Activity Test of Matoa (Pometia pinnata) Leaves Ethanol Extract Against Salmonella typhi and Bacillus subtilis Pagarra, Halifah; Taiyeb , Mushawwir; Junda , Muhammad; Musawira; Muhammad Naufal Syaiful Haq; Handayani , Resti
Bionature Vol. 26 No. 1 (2025): April-Juni 2025
Publisher : Jurusan Biologi Universitas Negeri Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35580/bionature.v26i1.7234

Abstract

This study aims to determine the antimicrobial activity of ethanol extract of matoa leaves (Pometia pinnata) against Salmonella typhi and Bacillus subtilis bacteria. This study began with preparing raw materials in the form of matoa leaves and then extracting the matoa leaves using the maceration method with 70% solvent. After that, the antimicrobial activity test was carried out using the disc diffusion method with four concentration variations, namely 5%, 7.5%, 10%, and 12.5% with a positive control in the form of 50 ppm amoxicillin and a negative control using distilled water, each treatment using three replications. The results in the form of inhibition zones formed were measured using calipers. The data obtained were then analyzed using SPSS 30 with the ANOVA test and further tests in the form of tests. The test results showed that 70% ethanol extract of matoa leaves had an effect on inhibiting the growth of Salmonella typhi and Bacillus subtilis bacteria. The largest inhibition zone in Salmonella typhi was found at a concentration of 7.5% with a diameter of 16.87 mm, while in Bacillus subtilis it was found at a concentration of 12.5% with a diameter of 12.22 mm. Keywords: Pometia pinnata, Antimicrobial activity, Salmonella typhi, Bacillus subtilis
The Effect of Matoa (Pometia Pinnata) Leaf Ethanol Extract on Reducing Blood Sugar Level in Male Mice (Mus Musculus) Induced by Alloxan Pagarra, Halifah; Salsabila, Shopia; Muis, Abd; Naufal Syaiful Haq, Muhammad
Bionature Vol. 26 No. 1 (2025): April-Juni 2025
Publisher : Jurusan Biologi Universitas Negeri Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35580/bionature.v26i1.7361

Abstract

This research aims to determine the total phenolic content and antioxidant activity of the ethanol extract from Matoa (Pometia pinnata) leaves as well as its effect on blood sugar levels in male mice (Mus musculus) induced with alloxan. To determine total phenolic content using the Folin-Ciocalteu method, and the DPPH method to measure antioxidant activity. The effect of Matoa leaves on blood sugar reduction in mice used a completely randomized design (CRD) with 15 mice divided into 5 treatments with 3 replications: namely P1 (Extract 125 mg/kg BW), P2 (Extract 250 mg/kg BW), P3 (Extract 500 mg/kg BW), K+ (Glibenclamide), and K- (Na-CMC 0.5%). Extraction used the maceration method with 70% ethanol solvent. The results of the total phenolic test showed the presence of phenolics with a value of 26.24872 mg GAE/g. The measurement of antioxidant activity yielded an IC50 value of 1.961 ppm. The results of testing the blood sugar levels showed that there was an effect of giving ethanol extract of Matoa (Pometia pinnata) leaves on reducing blood sugar levels in male mice (Mus musculus). Giving of ethanol extract of Matoa leaves at a dose of 250 mg/kg BW is effective in reducing blood sugar levels. So it can be concluded that the ethanol extract of Matoa leaves has an effect on reducing blood sugar levels in male mice
Shell Shape Variations and Sexual Dimorphism of Pomacea canaliculata using Geometric Morphometric Analysis Generol, Yashica; P. Magdugo, Rexie; Jhun Almadin , Florence
Bionature Vol. 26 No. 1 (2025): April-Juni 2025
Publisher : Jurusan Biologi Universitas Negeri Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35580/bionature.v26i1.7422

Abstract

Pomacea canaliculata or Golden Apple Snail (GAS) existed to be a rice pest in the Philippines and Asia. Likewise, geographic location also contributes to its increasing populations, thus making it invasive among freshwater habitats and rice field areas. This study was conducted to describe shell shape variations and sexual dimorphism among the populations of P. canaliculata. A total of 120 samples were randomly collected in the downhill and uphill geographic locations in Mambajao, Camiguin Island, Mindanao, Philippines. To determine the variations and sexual dimorphism in the shell shape of golden apple snail, coordinates were administered to relative warp analysis and the resulting data were subjected to Multivariate Analysis of Variance (MANOVA), Principal Component Analysis (PCA) and Canonical Variate Analysis (CVA). The results show statistically significant (P < 0.01). While male and female spire height, body size, and shell shape opening also show significant variations. These phenotypic distinctions could be associated with geographic isolation, predation, and a nutrient component of the gastropods. Thus, the importance of using geometric morphometric advances in describing sexual dimorphism in the shell shape of P. canaliculata.
Evaluation of Mango Leaf Extract as a Natural Preservative for Enhancing the Shelf Life and Quality of Milkfish Nurhayati B; Yahdiel Ronrong; Nani Kurnia; Alimuddin Ali
Bionature Vol. 26 No. 1 (2025): April-Juni 2025
Publisher : Jurusan Biologi Universitas Negeri Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35580/bionature.v26i1.7761

Abstract

This study investigates the potential of mango leaf extract as a natural preservative to extend the shelf life of milkfish (Chanos chanos) during storage. The extract was prepared using a standardized method and applied to milkfish fillets, followed by an analysis of its effects on microbial growth, sensory attributes, and overall shelf life. The milkfish samples were stored in the refrigerator, and periodic evaluations of sensory quality, including color, texture, odor, and taste, as well as microbiological parameters, were conducted. The findings of this study demonstrate that mango leaf extract effectively inhibits microbial growth and shows potential as a natural preservative for milkfish. The use of 1% mango leaf extract, especially ethanol-based, exhibited the highest microbial inhibition, significantly extending the shelf life and maintaining the quality of milkfish compared to untreated samples. These findings suggest that mango leaf extract can be an effective, natural alternative to chemical preservatives in extending the shelf life of milkfish, offering a promising solution for sustainable seafood preservation.
Microbial Assessment of Kappaphycus alvarezii and Caulerpa racemosa from Local Market Harvested in Barobo, Surigao Del Sur, Philippines Mae, Soijairah; A. Avila, Maria Cristina; M. Griño, Jonna Grace; Rexie P. Magdugo
Bionature Vol. 26 No. 1 (2025): April-Juni 2025
Publisher : Jurusan Biologi Universitas Negeri Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35580/bionature.v26i1.7178

Abstract

The practice of eating fresh seaweed is needed to assess the microbiological aspects of the seaweed, as it may contain pathogens hazardous to human health. This study aimed to assess the bacterial load of the fresh edible seaweeds Caulerpa racemosa and Kappaphycus alvarezii that are collected from the wild and then sold to public markets. All seaweed samples collected from wild and public markets are examined for aerobic bacteria (Aerobic Plate Count), coliforms (Coliform Count), Salmonella, and E. coli. The results revealed that the coliform, Salmonella, and E. coli were absent in all samples. The aerobic bacteria from the Caulerpa racemosa and Kappaphycus alvarezii collected from the public market were within the limits of the food quality standards set by JECFA, FDA-Philippines, and Food standards of Australia and New Zealand. As for Kappaphycus alvarezii collected from the wild revealed a higher Aerobic Plate count and has exceeded the limits on the food quality standard set by the agencies stated. This study concludes that Caulerpa racemosa and Kappaphycus alvarezii from the public market conform to the requirements of the food quality standards and that sanitary handling of edible seaweeds sold to public markets was properly practiced.

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