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Annales Bogorienses
Published by Badan Riset dan Inovasi Nasional
ISSN : 05178452     EISSN : 24077518     DOI : https://doi.org/10.55981/ann.bogor
Core Subject :
Annales Bogorienses aims to disseminate high-quality scientific research in the field of life sciences, with a strong emphasis on advancing knowledge and applications in biotechnology, molecular biology, biochemistry, bioinformatics, and bioengineering. The journal serves as a platform for researchers, academicians, and practitioners to share original findings, innovative methodologies, and critical reviews that contribute to scientific progress and sustainable development. The journal covers research in biotechnology, molecular biology, biochemistry, bioinformatics, and bioengineering. It publishes original research articles, reviews, and short communications, and is committed to rigorous peer review and open access for the widest possible dissemination of scientific knowledge.
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Articles 203 Documents
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Present Status and Challenges on Agricultural Microbe Bio-Prospecting in Indonesia Endang Sukara
Annales Bogorienses Vol. 11 No. 1 (2007): Annales Bogorienses
Publisher : National Research and Innovation Agency (BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ann.bogor.2007.v11.n1.31-39

Abstract

Food security is one of the essential needs which can never be ignored by any society, including in particular Indonesia, as the world population is expected to rise to around 10 billion marked by 2025. The problem is that the agriculture in developed and developing countries that follow the path of developed countries experiences create a range of serious environmental degradation. An intensive agricultural production through the use of pesticide and herbicides, forest clearing and continued use of land resources for the agricultural purposes are not sustainable practices. In consequences, degradation of ecosystem, oil erosion, and low productivity of land, poor water quality and lost of biological diversity (including microbial and genetic resources) occurred at alarming state. Meanwhile, the efforts in addressing the above situation is traditionally focused on plant phen types (breeding. plant genetic engineering, etc.) but the important role of microbial communities that interact with plants to influence plant health and productivity has been largely ignored. The interaction of microbes with plants and its relevant to soil fertility and productivity, the biocontrol properties of microbes and challenges toward sustainable agriculture is highlighted.
EDITOR'S PREFACE Puspita Lisdiyanti
Annales Bogorienses Vol. 12 No. 1 (2008): Annales Bogorienses
Publisher : National Research and Innovation Agency (BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

In this issue, 5 original research papers are presented. The fIrst paper explains the authors attempts to purify and characterize an extracellular enantio seJective lipase from Aspergillus niger, an important component for the manufacture of c1ural drugs such asibuprofen. The second papers describe the identification of a highly abundant lectin in Arabidopsis thaliana. with potential application for biopesticides against nematode. In the third paper, the authors explain an association of a polymorphism in the a-lactoglobulingene on milk yield and quality in local sheep. The fourth paper describes the authors efforts to construct a synthetic human errythropoietin (EPO) in an attempt to obtain high-yield production of the protein in yeast. And the fifth papers describes the newly develop a method to do micropropagation of buah merah (Pandanus conoideus Lam), which is a potentially important source for anticancer medication. The Editor
Rapid Purification and Partial Characterization of an Extracellular Enantioselective Lipase from Aspergillus niger Domininggus Malle; Gina Garingan; Milagros Peralta; Maria Jamela Revilleza
Annales Bogorienses Vol. 12 No. 1 (2008): Annales Bogorienses
Publisher : National Research and Innovation Agency (BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ann.bogor.2008.v12.n1.1-8

Abstract

Lipases [EC. 3. 1.1 .3] are capable of hydro lyzing ester bonds of triglycerides and have received much attention from protein researchers after these enzymes have shown potential applications such us, in the manufacture of commercially important crural drugs due to their enantioselectivity properties. A novel extracellular lipase produced by Aspergillus niger was purified and partially characterized. Using the culture medium as crude extract, the Lipase was purified to 25 .7 folds after Ultrafiltration and DEAE ion exchange chromatography. The enzyme was characterized to have optimum activity at neutral pH and at 30-35°C, while its kinetic parameters, Vmax and Km, were determined to be 1.80 U/mL/min and 0.2 mL, respectively. SDS-PAGE analysis revealed the presence of at least two bands (±87 and ±72 kDa), while native PAGE showed a single band. The two bands could represent subunits in a complex or isoforms of the enzyme. The use of the enzyme in the hydrolysis of a racemic mixture of 2-arylpropionic butyl ester analyzed after HPLC demonstrated an apparent predominant enantioselectivity for the S (+) enantiomer. Thus, this lipase is a promising enzyme for the chiral drug preparation of single enantiomer of 2-arylpropionic acid (ibuprofen).
A Highly Abundant Lectin Protein in Arabidopsis thaliana Confers Resistance Against Pathogens Maria Prihtamala Omega; Skye Thomas-Hall; Peer Schenk; Bostjan Kobe
Annales Bogorienses Vol. 12 No. 1 (2008): Annales Bogorienses
Publisher : National Research and Innovation Agency (BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ann.bogor.2008.v12.n1.10-15

Abstract

Lectins are glycoproteins that recognize and bind to specific carbohydrates. They are involved in a range of biological functions, such as plant defence, storage proteins seed germination, and plant microbe interactions. Lectin 3.1 (At3g15356) is a protein in plant model, Arabidopsis thaliana, that has been shown to be up-regulated in all defence pathways especially in response to methyl ester jasmonale (MJ). All that was known about the gene was that it had go d homology to the beta domain of legume lectins. That aim of this project was to characterize the structure and function of the lectin protein using CD spectra and X-ray crystallography. AT-DNA insertion line for the lectin gene and a number of 35S over-expression line that had varying levels of expression had been generated, but none of these showed any obvious phenotype. Two protein bands were observed on comassie stained SDS-PAGE gels in the over-expression lines and in MJ induced wild-type (WT). The two protein bands represented two isoforms of the lectin 3.1 protein; in a glycosylation assay the larger protein band was shown to be heavily glycosylated. A nematode (M. incognita) disease assay discovered that the lectin overexpression lines had les nematode eggs compared to that of the WT and that the insertion line bad more nematode eggs than the WT. This data provide evidence that lectin 3.1 improves plant resistance against M. incognita infection. interestingly, the nematode gut lining contains fucose with which lectin 3.1 binds to.
Association between Polymorphism of β-Lactoglobulin Gene on Milk Yield and Quality in Local Sheep at Jonggol Animal Science Teaching and Research Unit (JASTRU) Cece Sumantri; Nurhayati D. Nurhayati; Ahmad Farajallah; A. Anggraeni Anggraeni
Annales Bogorienses Vol. 12 No. 1 (2008): Annales Bogorienses
Publisher : National Research and Innovation Agency (BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ann.bogor.2008.v12.n1.17-24

Abstract

The aims of this study were to identify the effects of polymorphism of b-lactoglobulin gene on milk yield and percentage of milk protein and fat in local sheep. A total number of 83 heads of lactating ewes raised under an extensive management at Jonggol Animal Science Teaching and Research Unit (JASTRU) of the Faculty of Animal Science, Bogor Agricultural University were studied. Research activities were carried out through some steps involving blood collection. DNA isolation, DNA amplification, and separation of DNA fragments by electrophoresis with silver staining method. By using polymerase chain reaction (PCR) then genotyped by single strand conformation polymorphism technique (SSCP), it was successfully amplified a fragment length at 420 bp in b-lactoglobulin genelocated on the exon 7. The electrophoresis pattern revealed 5 types of b-lactoglobulin gene and designated as A, B, C, D, and E types. The proportion for those respective 5 type from the highest to the lowest were for A (27.71%), C (16.87%), D (12.05%), E (10.84%), and B (9.64%), respectively. There were no significant effect of polymorphisms in b-lactoglobulin gene on both milk yield and quality in local sheep at JASTRU.
Construction of an EPO (Human-Erythropoietin) Synthetic Gene Through a Recurvise-PCR Method Asrul Muhamad Fuad; Tutus Gusdinar; Debbie Sofie Retnoningrum; Dessy Natalia
Annales Bogorienses Vol. 12 No. 1 (2008): Annales Bogorienses
Publisher : National Research and Innovation Agency (BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ann.bogor.2008.v12.n1.25-38

Abstract

Human erythropoietin (hEPO) is an important glycoprotein in human that is coded by a single gene named EPO (erythropoietin). EPO is a glycoprotein hormone that promotes erythropoiesis, which is the formation process of mature red blood cell (erythr eyte ) in human bodies. It is widely used for treatment of anemia in patient with chronic renal failure. Therefore EPO has been classified as hematopoietic cytokine. Recombinant hEPO (rhEPO) has been commercially available, such a Epogen. It is produced in mammalian cell, such as CHO (Chinese hamster ovary) ceil for the reason of it· complex structure as a glyco-protein. In an effort to use and optimize heterologous EPO gene expression in an alternative eukaryotic host cells such as yeast, an EPO synthetic gene (EPOsyn) was constructed. The synthetic gene had been designed to contain optimal Pichia pastoris codon usage. It had been constructed by a recursive-PCR method in two-step PCR reactions. The gene was assembled from 8 single strands synthetic oligonucleotides having an average length of 90 nt with 20 to 30 overlap region between two adjacent oligos. The synthetic gene has less GC content (45.31%) compared its native (human) gene (59.08%). The synthetic gene has been cloned in pCR2.1 cloning plasmid and sequenced. From 8 independent clones, it was revealed that the error rate was 1.59%, in which 1.42% was due to deletions and 0.17% due to substitutions. Design of the gene sequences, construction method and DNA sequence analysis of the gene will be discussed in this paper.
In Vitro Propagation of Buah Merah (Pandanus Conoideus Lam) Through Lateral Bud Proliferation Maria Imelda; Aida Wulansari; Sumarnie Sumarnie
Annales Bogorienses Vol. 12 No. 1 (2008): Annales Bogorienses
Publisher : National Research and Innovation Agency (BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ann.bogor.2008.v12.n1.39-43

Abstract

Pandanus conoideus Lam or 'Buah merah' of the Pandanaceae is a native to East Indonesia, particularly Papua and North Maluku. Traditionally the fruits are used for health promotion and maintenance as well as for curing several illnesses. Recently, it has been reported that the fruit its are potential for cancer medication. As a result, there has been an overexploitation of the plants from their habitats. In order to anticipate their possible disappearance due to overexploitation in the wild, an efficient and effective technology for the mass propagation, conservation, and cultivation of these plant should be developed. Generally buah merah is propagated vegetatively by offshoots and stem cuttings or generatively by seeds. Micropropagation has many advantages over the conventional method because the technique allows mass clonal and pathogen-free production of plants at a high rate of multiplication all year round. In this research the effects of 0.1-0.2 mg/L thidiazuron (TDZ), 0.5-1.0 mg/L benzyl amino purine (BAP), and 0.25-0.5 mg/L kinetin (KN) on shoot bud induction and proliferation of P. conoideus were investigated using nodal infections or lateral buds of P. conoideus on modified Murashige and Skoog (MS) medium. Shoots were rooted on MS medium without plant growth regulator (PGR). The result showed that lateral bud of Pandanus start d to initiate growth after 4-7 days in culture. The best medium for shoot proliferation was MS containing either 0.5 mg/l BAP with 0.1 mg/l TDZ or 1 mg/l BAP with 0.5 mg/l KN giving a multiplication rate of 16.5 shootlets per shoot bud explant after 8 weeks. Rooting of shoots was successfully conducted on MS medium without PGR. Acclimatization of rooted plantlets was achieved on a mixed medium of cocopeat and soil (1:1).
Effect of Culture Conditions on Phytase Production by Aspergillus ficuum in Solid-state Fermentation Using Rice Bran as Substrate Wien Kusharyoto; Martha Sari; Asep Muhamad Ridwanuloh
Annales Bogorienses Vol. 13 No. 1 (2009): Annales Bogorienses
Publisher : National Research and Innovation Agency (BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ann.bogor.2009.v13.n1.1-11

Abstract

Phytic acid is an antinutritional factor that forms 1–2% of most of the seeds and their co-products representing more than 60% of their total phosphorus. Monogastric and agastric animals are unable to utilize phytate phosphorus either due to lack of or insufficient amount of phytate degrading enzymes. Phytases (myo-inositol hexakisphosphate-phosphohydrolase) are a special class of phosphatases that catalyze the hydrolysis of phytic acid in a stepwise manner to lower inositol phosphates, myo-inositol and inorganic phosphate. Phytases are found naturally in plants and microorganisms and a sizeable number of phytases have been purified and characterized from various fungi, yeasts, and bacteria. The present investigation involves studies on the effect of moisture content, pH value and different media ingredients such as carbon, nitrogen, and surfactants on the production of phytase by the fungus Aspergillus ficuum DSM 932 in solid-state fermentation (SSF) using rice bran as substrate. The production of phytase by SSF was favored, when the fungus was grown at a moisture content of 60% and pH 7.0, resulted in a phytase activity of 5.2 units/g dry substrate. There was a 20% increase in phytase yield in the presence of sucrose in SSF medium, while glucose and fructose were not effective in enhancing the phytase activity when used individually. Yeast extract was found to be a favorable nitrogen source for phytase production by SSF, which resulted in a 20% increase in phytase activity. There was no significant effect in increasing phytase production with the use of either soy peptone or tryptic soy as nitrogen source. Approximately 30% inhibition in phytase activity was shown in the presence of the surfactant Tween-80 or Triton X-100 in the SSF. By supplementing rice bran with sucrose and yeast extract, and performing the SSF in tray bioreactors, a phytase activity of 6.76 units/g dry substrate could be obtained.
Agrobacterium-mediated Transformation of Banana Musa acuminata AA cv "“Mas Lampung” with hpt Gene Using Sterile Corm as Target Tissue Amy Estiati; Ade Nena; Witjaksono Witjaksono
Annales Bogorienses Vol. 13 No. 1 (2009): Annales Bogorienses
Publisher : National Research and Innovation Agency (BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ann.bogor.2009.v13.n1.12-20

Abstract

The protocol for Agrobacterium-mediated transformation of local banana plants cv “Mas Lampung” (AA) has been established. A selectable marker gene (hpt) has been used to study the transformation using in vitro corm slices as target tissues. Banana in vitro corm slices were co-cultivated with the EHA105 strain of Agrobacterium tumefaciens harbouring binary vector pCAMBIA 1301 containing hygromycin resistance gene (hpt) as a selectable marker and intron-containing β-Glucuronidase (gus-intron) gene as a reporter gene driven by CaMV 35S promoter. Polymerase Chain Reaction (PCR) were used to examine the existence of hpt gene in plants resulted from the transformation. Using primer pairs specific for hpt gene, our PCR analysis on leaves showed the presence of the hpt transgene in banana transgenic plants at first generation (T0) of transformation. To prove the existence of hpt gene in the fruits of transgenic banana plants, PCR analysis were also carried out. The data showed that the hpt gene could be amplified from banana fruits of tested samples. These result demonstrates that the Agrobacterium-mediated transformation method used in this experiment has been successful to transfer gene into banana plants. Thus, the transformation method reported here could be used as a standard protocol to transfer another useful genes into local banana plants cv. “Mas Lampung”. Furthermore, the presence of transgene in fruits of banana transgenic plants is important achievement especially for transgene that is expected to be expressed in the fruit including to introduce vaccine genes into banana fruits for edible vaccine.
Propagation of Sukun (Artocarpus altilis (Parkinson) Fosberg) Through In Vitro Shoot Proliferation Maria Imelda; Aida Wulansari; Laela Sari
Annales Bogorienses Vol. 13 No. 1 (2009): Annales Bogorienses
Publisher : National Research and Innovation Agency (BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ann.bogor.2009.v13.n1.21-28

Abstract

Sukun (Artocarpus altilis (Parkinson) Fosberg) of the Moraceaae is a big tree which can grow to 15-20 m in height and native to the Asia-Pasific region. Beside its delicious fruit, sukun is also known as a traditional herbal medicinal plant in the region, including Indonesia. Nearly all parts of the plant, such as roots, stems and leaves are believed by local communities to be capable of curing liver disease, hypertension, cardiac arrest, toothache, renal problem and even skin itchiness. The collaborative research between LIPI and PR China, on developing herbal medicines indicated that sukun has a great potential for treating cardiovascular disease. However, the availability of raw materials still poses a big constraint for the industry of herbal medicines. Generally, sukun is propagated by root or stem cuttings, since in Indonesia sukun does not produce any seeds. However such method only produces limited planting materials. In general tissue culture propagated plants have many advantages, namely being clonal, free from pest and diseases, more uniform, and allowing a high rate of plant multiplication. Therefore, the technique for sukun propagation has been developed by the LIPI Research Centre for Biotechnology. In this research the effects of 1-5 mg/l benzyl amino purine (BAP) and 20-40 mg/l adenine sulphate (AS)on shoot bud proliferation were investigated using lateral shoot buds on a modified Murashige and Skoog (MS) medium with addition of 150 ml/l coconut water (CW). Shoots were rooted on MS medium without plant growth regulators (PGRs). The results showed that the best medium for in vitro shoot proliferation was a modified MS medium containing 2 mg/l BAP, 40 mg/l AS and 150 ml/l CW. The best medium for rooting is MS medium containing 1 mg indole butyric acid (IBA), producing roots within 3 weeks.

Page 8 of 21 | Total Record : 203

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