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Jurnal Bioteknologi & Biosains Indonesia
Published by Badan Riset dan Inovasi Nasional
ISSN : 24422606     EISSN : 2548611X     DOI : https://doi.org/10.55981/jbbi
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology
JBBI is published twice annually and provide scientific publication medium for researchers, engineers, practitioners, academicians, and observers in the field related to biotechnology and bioscience. This journal accepts original research papers, review articles, case studies, and short communications. The articles published are peer-reviewed by no less than two referees, and cover various biotechnology subjects related to the field of agriculture, industry, health, environment, bioinformatics, as well as life sciences in general. JBBI terbit dua kali setahun dan menyediakan media penerbitan ilmiah bagi para peneliti, insinyur, praktisi, akademisi, dan pemerhati di bidang yang terkait dengan bioteknologi dan biosains. Jurnal ini menerima naskah asli hasil penelitian, naskah ulasan, studi kasus, dan komunikasi singkat. Naskah yang diterbitkan adalah peer-review oleh tidak kurang dari dua orang penelaah, dan mencakup berbagai cabang bioteknologi yang terkait dengan bidang pertanian, industri, kesehatan, lingkungan, bioinformatika, serta ilmu kehidupan pada umumnya.
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 146 Documents
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BIOREMEDIATION FORMULA: SPORULATION INDUCTION AND ENDOSPORE STABILITY OF Bacillus pseudomycoides RAY21 IN BIO-OSD Simatupang, Yeni Veronika; Fauzi, Anas Miftah; Yani, Moh.; Hambali, Erliza
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 2 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.12956

Abstract

Oil-based soil pollution is a pressing global environmental issue and has serious impacts on ecosystems and human health. Hydrocarbon compounds, especially heavy fractions, are persistent and difficult to decompose naturally in a short time, thus requiring intervention to accelerate the environmental recovery process. This research focuses on developing strategies to increase the effectiveness of oil-contaminated soil degradation. The development of a palm oil-based oil spill dispersant (Bio-OSD) combined with bacterial endospores of Bacillus pseudomycoides RAY21 offers an innovative approach to bioremediation. This particular bacterial endospore was selected for its capacity to sustain the viability of a potent biological agent until the point of application. The bacterial growth process was monitored to determine the optimal harvesting time by measuring optical density (OD600) at hourly intervals using a spectrophotometer until the stationary phase was reached. Following the harvesting of bacterial cells, various stressors were applied to stimulate sporulation, which included high-temperature incubation (80°C), nutrient deprivation, and their synergistic combination. The efficacy of the sporulation process was validated through endospore staining with malachite green. The resultant endospore pellets were integrated into the Bio-OSD and subjected to viability assessments every three days over a 24-day period using the Total Plate Count (TPC) method. The biodegradative capacity of the Bio-OSD containing endospores was evaluated through the analysis of Total Petroleum Hydrocarbon (TPH) degradation in artificially contaminated soils (3%) across a 28-day incubation. Findings indicated that optimal bacterial cell production occurred in Nutrient Broth, with peak harvesting at 40 hours. Moreover, the most effective sporulation was attained under combined stress conditions specifically, exposure to high temperature (80 °C for 24 hours) concomitant with nutrient deprivation (NaCl 0,85%) as demonstrated by the presence of green-stained endospores. This study provides evidence that Bio-OSD functions effectively as a stable carrier medium for bacterial endospores over a period of up to 24 days. The Bio-OSD formulation incorporating Bacillus pseudomycoides RAY21 endospores demonstrated the capability to degrade 78% of Total Petroleum Hydrocarbons (TPH) in petroleum-contaminated soil after 28 days of incubation. This degradation efficiency was significantly superior to that of the control treatment, which achieved a mere 32% degradation under the same laboratory conditions. To enhance the understanding and application of this approach, further investigations are recommended to be conducted on a larger scale, along with optimization analyses, to facilitate the development of more sustainable environmental remediation.
ISOLATION, SCREENING, AND ANTIBACTERIAL ACTIVITY OF ENDOPHYTIC FUNGI ASSOCIATED WITH Acanthus ilicifolius L. IN INHIBITING THE GROWTH OF Methicillin-Resistant Staphylococcus aureus (MRSA) Wiradana, Putu Angga; Anak Agung Ayu Putri Permatasari; I Gede Widhiantara; I Made Wisnu Adhi Putra; Ni Kadek Yunita Sari; Novaria Sari Dewi Panjaitan; Sri Puji Astuti Wahyuningsih; Mochammad Aqilah Herdiansyah
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 2 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.13090

Abstract

This study examines the phytochemical composition and antibacterial activity of endophytic fungi linked to the mangrove plant A. ilicifolius against methicillin-resistant Staphylococcus aureus (MRSA) in order to determine their bioprospecting potential.  The surface-sterilized root, stem, and leaf tissues of A. ilicifolius yielded endophytic fungi. The workflow consisted of screening for viable isolates, culture under controlled circumstances, and molecular identification of the Internal Transcribed Spacer (ITS) section of fungal rDNA. Antibacterial activity was determined using agar well diffusion tests after extraction with methanol, ethyl acetate, and n-hexane solvent fractions. A total of 31 endophytic fungal isolates were recovered: 9 from roots, 15 from stems, and 7 from leaves. Among these, 5 isolates from roots (16.1%), 8 from stems (25.8%), and 5 from leaves (16.1%) exhibited inhibitory effects against MRSA. Isolate AK5, derived from root tissue, demonstrated the highest antibacterial activity and was molecularly identified as Chaetomium globosum strain NW 24 (Accession No. MN326469.1). The isolate revealed optimal growth at pH 5–6, with the maximum wet mycelial biomass (29.73 g) achieved on day 24 under shaker incubation. The methanol and ethyl acetate fractions had a considerably greater anti-MRSA efficacy than the n-hexane fraction. Phytochemical analysis of the methanolic extract indicated the presence of several bioactive components, including phenolics, tannins, flavonoids, saponins, alkaloids, and terpenoids, indicating that these constituents contribute to the reported antibacterial effects. Overall, our findings highlight the potential of A. ilicifolius-derived endophytic fungi as alternate sources of bioactive compounds for treating antibiotic-resistant infections, specifically MRSA.
UTILIZATION OF INDONESIAN STRAIN ACTINOBACTERIA TO FACE THE THREAT OF BIOFILM AS THE MAIN CAUSE OF HEALTH CARE ASSOCIATED INFECTION Cahyo, Bian Dwi; Retnowati, Dwi; Lisdiyanti, Puspita
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 2 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.13111

Abstract

Health Care Associated Infection (HCAI) is one of the global infectious diseases that is expected to cause around 10 million deaths by 2050. One of the main causes is biofilm, an exopolysaccharide layer formed by bacteria, often found on medical equipment such as catheters, and has high resistance to antibiotics. Prevention efforts can be made through the search for antibiofilm compounds. Actinobacteria are known to produce potential bioactive compounds. This study utilized Actinobacteria strains from the waters of Bitung, North Sulawesi (code BT-023-026) to explore their antibiofilm potential through isolation, laboratory tests, and genetic analysis, followed by expert confirmation in the fields of intelligence, BPOM, and microbiology. The results show the great potential of BT-023-026 as an antibiofilm agent to prevent antibiotic resistance due to HCAI, while playing a strategic role in biodefense and strengthening health security through early warning and early detection.
METAGENOMIC EXPLORATION OF ECO-ENZYME PRODUCTION: UNVEILING ENZYMATIC ACTIVITIES AND MICROBIOTA ABUNDANCE FOR SUSTAINABLE ORGANIC WASTE MANAGEMENT Prakoso, Haryo Tejo; Permatasari, Galuh Wening; Dimawarnita, Firda; Kalbuadi, Donny
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 2 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.13247

Abstract

Globally, organic waste is steadily increasing, raising serious environmental challenges. A promising solution is the conversion of organic waste into Eco-Enzyme (EE), a fermented product widely applied in wastewater treatment, soil improvement, and fertilization. Despite its potential, few studies have characterized EE from a metagenomic perspective. This study examined enzymatic activities and microbial communities during EE production. EE was prepared from pineapple and orange peels mixed with palm sugar and water, then fermented for 90 days. The mixture was analyzed for enzymatic activities including amylase, cellulase, protease, chitinase, lignin and Mn peroxidase, peroxidase, glucanase, and catalase, and linked to waste degradation potential. Metagenomic sequencing using Oxford Nanopore Technology revealed Terrabacteria (83%), dominated by Lacticaseibacillus and Lentilactobacillus, and Proteobacteria (17%) with Acetobacter fabarum prevalent in EE sample. The highest activity was catalase (5107 mU/ml), followed by chitinase, protease, and amylase. These results highlight EE’s microbial and enzymatic complexity, reinforcing its role in sustainable organic waste management.
THE IMPACT OF MOBILE HEALTH ON HEALTHY LIFESTYLE COMPLIANCE AND QUALITY OF LIFE OF DIABETES MELLITUS PATIENTS: A SCOPING REVIEW Santosa, Winanda Rizki Bagus; Sujatmiko, Sujatmiko; Astutik, Wahyu Sri; Manohara, Galuh Dharanindya Ica; Nuraini, Ida Rizqa
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 2 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.13261

Abstract

Compliance with a healthy lifestyle and improving the quality of life of patients can be done through education with mobile health to prevent long-term complications in Diabetes Mellitus (DM) patients. Aims of the research this is to know the impact of mobile health on healthy lifestyle compliance and the quality of life of DM patients. In this research, these are PubMed, CINAHL, and Scopus. The search review uses the keywords "Diabetes Mellitus," "Mobile Health," "Healthy Lifestyle Compliance," and "Quality of Life" in the articles. Based on the results, 12 out of 305 articles were identified as feasible. Analysis showed that mobile health can be used for improving healthy lifestyle compliance, and improving the quality of life among DM patients. The time DM patients used mobile health application for education varies greatly. Mobile health has great potential to help improve compliance with lifestyle health, and quality of life for DM patients.
BIBLIOMETRIC ANALYSIS OF PATIENT OUTCOMES AND SATISFACTION IN ROBOTIC-ASSISTED HIP AND KNEE JOINT REPLACEMENT SURGERY Soetjahjo, Bintang; Santoso, Asep; Mercy, Grendi; Adriansyah, Denny; Angeline, Agiona; Sadewa, Daffa
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 2 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.13395

Abstract

Robotic-assisted hip and knee arthroplasty has gained popularity in orthopedic surgery due to its precision, faster recovery, and improved alignment, potentially enhancing patient outcomes. Despite mixed and heterogeneous evidence, the demand for these procedures continues to rise. Therefore, bibliometric analysis is essential to direct research towards patient satisfaction and generate more reliable and significant evidence proving the true benefits of robotic surgery for patients, rather than merely technical success. This study employed bibliometric analysis to explore trends and evaluate publications on patient outcomes and satisfaction in robotic-assisted hip and knee surgery using the Scopus database. The analysis identified core concepts, trends, and research connections, providing insights into emerging areas in the field. The bibliometric analysis of 324 documents from 2007 to 2025 reveals a significant increase in research, focusing on surgical precision, rehabilitation, and long-term effectiveness. While countries like the US, UK, and China are leading contributors, there is a notable lack of similar research in ASEAN countries, particularly Indonesia. Future research should address gaps in areas like surgical revision, patient perception, and improving patient-reported outcomes.
THE POTENTIAL OF Zinnia elegans AND Bacillus sp. as Lead (Pb) BIOREMEDIATION AGENTS Febiola, Regina Rosari; Prasetyawati, Endang Triwahyu; Nirwanto , Herry
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 2 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.13482

Abstract

Lead (Pb) pollution in soil is a serious problem that reduces crop productivity. One approach to address this issue is through bioremediation and Phytoremediation, using microorganism and plants that can tolerate and bind heavy metals. This study examine the potential of Zinnia elegans (Jacq.) and Bacillus sp., and in combination to reduce Pb in soil. The experiment lasted 60 days using a completely randomized design with six treatments: P0 (control, Pb-contaminated soil without plants or bacteria), P1 (Z. elegans only), P2 (Bacillus sp. only), P3 (combination), P4 (non-contaminated soil with Z. elegans and Bacillus sp.), and P5 (non-contaminated soil with Z. elegans only). The results showed that treatment P3 reduced Pb concentration by 62.31 ppm, while no significant differences were observed among treatments in terms of plant growth parameters. Combining Z. elegans with Bacillus sp. may serve as a practical approach to enhance Pb remediation in contaminated soils.
MORINGA OLEIFERA LEAF AS A POTENTIAL ANTITHROMBOTIC AGENT: AN IN VITRO EVALUATION Ayanti, Bio Putri; Nurbidayah, Nurbidayah; Amalia, Nurul; Azzahra, Syafina; Amini, Amalia
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 2 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.13503

Abstract

Cardiovascular disease (CVD) is a major cause of morbidity and mortality worldwide, one of which is triggered by an imbalance between fibrin formation and fibrinolysis processes, leading to fibrin accumulation that can lead to thrombosis. Commercial thrombolytic agents are currently widely used, but their use is known to cause serious side effects often and have limited effectiveness. This gap encourages initial screening of natural materials as an effort to explore the potential of new thrombolytic agents in the future. This study aims to evaluate the potential of Moringa oleifera leaf filtrate at concentrations of 25%, 50%, 75%, and 100% as an antithrombotic agent through qualitative identification of secondary metabolites and testing of thrombolytic activity and anticoagulant ability in vitro. The results showed that Moringa leaf filtrate at a concentration of 25% was able to lyse blood clots by 60%, compared to the positive control nattokinase (82%), with a relative effectiveness of 71.7%. In vitro anticoagulant activity testing showed an extension of blood clotting time from 10 minutes (control) to 35 minutes in the treatment. ANOVA analysis showed a significant difference between concentrations (p < 0.05). These findings support the initial potential of this natural product as a candidate antithrombotic agent and provide a scientific basis for further research.
COMPARATIVE pH ANALYSIS: HOMEMADE COLD CLAY VERSUS COMMERCIAL CLAY FOR CHILD SAFETY Harjani, Centaury; Aditiyarini, Dwi; Prakasita, Vinsa Cantya
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 2 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.13507

Abstract

Cold clay, as a non-baking children's play medium, needs to be assessed for its chemical and physical safety, particularly its pH parameters, to ensure safety for children's skin and environmental sustainability. This study aims to characterize the pH of cold clay by comparing homemade and manufactured products. A total of six samples were tested: one homemade (based on polyvinyl acetate, cornstarch, and additives) and five manufactured, colorful ones. The pH measurements were performed using a digital pH meter with three replications, then statistically analyzed using an independent t-test. The results showed a highly significant difference (p < 0.001) between the two groups. Homemade samples had an average pH of 4.75 ± 0.06 (acidic), while manufactured samples ranged from 7.16–7.53 (neutral to slightly alkaline). From a dermatological perspective, homemade pH, which is closer to the natural pH of children's skin (~5.5), is considered safer for long-term contact because it does not disrupt the acid mantle. In contrast, the neutral pH of manufactured products showed better chemical stability but potentially poses challenges in waste management. These findings emphasize the importance of pH characterization in selecting safe materials for the intended application. The study recommends consideration of pH parameters for parents, educators, and industry, as well as the need for further studies on toxicity and environmental impacts to ensure overall safety.
IDENTIFICATION OF BACTERIA FROM FOOD COURT WASTEWATER BASED ON 16S rRNA GENE SEQUENCES Elasari , Indah Nur; Abdillah , Rihan Ali; Dewa , Satria Prasna; Widjaja, Jafni Amanda Kinarossa; Lisdiana, Lisa
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 2 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.13541

Abstract

Environmental safety and public health are greatly influenced by the microbiological elements such as bacterial community, where these organisms can be used as indicators of environmental quality and sources of potential biological hazards. The identification of bacteria in wastewater is an important aspect of environmental microbiology studies. The purpose of this study was to identify the bacterial species in food court wastewater at State University of Surabaya (UNESA) Campus 1. The methods used were exploratory, including bacterial cultivation, DNA extraction, quantitative and qualitative DNA analysis, amplification of 16S rRNA gene fragments, sequencing, and phylogenetic tree reconstruction. From phylogenetic analysis, five test isolates were related to the families Enterobacteriaceae, Aeromonadaceae, Chromatiaceae, and Halomonadaceae. Isolates BU4 and BU5 were most closely related to genus Aeromonas with a similarity of 98.60% and 97.27%, respectively, while isolate BU3 was closely related to genus Klebsiella with a similarity of 96.91%. Meanwhile, isolates BU1 and BU2 cannot yet be identified into a bacterial genus. The results of this study provide important information regarding the prediction of pathogenic bacteria types, which can serve as the basis for recommending further action to prevent disease, improve water quality, and increase the sterility of the food court area.

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