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Jurnal Bioteknologi & Biosains Indonesia
Published by Badan Riset dan Inovasi Nasional
ISSN : 24422606     EISSN : 2548611X     DOI : https://doi.org/10.55981/jbbi
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology
JBBI is published twice annually and provide scientific publication medium for researchers, engineers, practitioners, academicians, and observers in the field related to biotechnology and bioscience. This journal accepts original research papers, review articles, case studies, and short communications. The articles published are peer-reviewed by no less than two referees, and cover various biotechnology subjects related to the field of agriculture, industry, health, environment, bioinformatics, as well as life sciences in general. JBBI terbit dua kali setahun dan menyediakan media penerbitan ilmiah bagi para peneliti, insinyur, praktisi, akademisi, dan pemerhati di bidang yang terkait dengan bioteknologi dan biosains. Jurnal ini menerima naskah asli hasil penelitian, naskah ulasan, studi kasus, dan komunikasi singkat. Naskah yang diterbitkan adalah peer-review oleh tidak kurang dari dua orang penelaah, dan mencakup berbagai cabang bioteknologi yang terkait dengan bidang pertanian, industri, kesehatan, lingkungan, bioinformatika, serta ilmu kehidupan pada umumnya.
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 146 Documents
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EFFECTIVENESS OF HYDROCOLLOID USE ON WOUND HEALING PROCESS IN WOUND PATIENTS AT HANIFAH MEDIKA CENTER PRIMARY CLINIC Naziyah, Naziyah; Maryati, Maryati; Hidayat, Rizki
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 2 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.13635

Abstract

Wounds due to tissue damage can be caused by pressure, friction, extreme temperatures, or infection. WHO from 2022 recorded a wound prevalence of 3.50 per 1000 population, with the majority of cases resulting from surgery or trauma (48%). In Indonesia, around 35% of the population experiences wounds, with pressure ulcers reaching 20%. Nurses play a crucial role in wound care, including the selection of appropriate dressings. Hydrocolloid is a modern dressing that protects wounds from water, air, and bacteria, and creates a moist environment to accelerate healing. This study evaluates the effectiveness of Hydrocolloid in accelerating the wound healing process at the Hanifah Medika Center Primary Clinic. Aim to examine the effect of using the hydrocolloid on wound healing process in wound patient at Hanifah Medica Center clinic. Methods This study was a quantitative design with the Quasi-Experiment methode without a Control Group Pre-Test - Post-Test. In this design, the intervention is given to one group without a control group for comparison. Results: The results showed that mean of pre-test Winner scale score of 28.23 ± 3.093 and a post-test score of 15.63 ± 3.090. The research results indicate a difference in the Winner scale assessment scores between the pre-test and post-test, with a P-value of 0.000 (p-<0.05). Conclusion There was a decrease in wound assessment scores using the Winner scale, with a pre-test score of 28.23 ± 3.093 and a post-test score of 15.63 ± 3.090. which means that the use of Hydrocolloid is effective in the wound healing process. Suggestion: It is suggested that nurses, healthcare institutions, and wound patients can use Hydrocolloid as a dressing continuously. The three of them become one of the efforts in the wound healing process.
HEMATOLOGY AND URINAL ANALYSIS PROFILE IN DIABETES MELLITUS Wahyuni, Rizka Ayu; Nurbidayah, Nurbidayah; Amalia, Nurul; Al Fitri, Aulia Syafa; Mubarakah, Noor Zahwa
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 2 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.13645

Abstract

Type 2 Diabetes Mellitus (T2DM) constitutes a chronic metabolic disorder characterized by insulin resistance and consequential hyperglycemia. This state of elevated blood glucose can precipitate various complications, potentially influencing hematological parameters and urinary composition. This study aims to delineate the demographic characteristics, hematological profiles, and urinalysis results of individuals diagnosed with T2DM. Employing an observational descriptive design with a cross-sectional approach, the research examined T2DM patients who underwent laboratory testing at RSD Idaman Banjarbaru. The findings indicate that, from a total of 40 patients, the majority (n=18, 45%) belonged to the 46–55-year age cohort. Regarding gender distribution, female patients were more predominant, comprising 29 individuals (72.5%), compared to 11 male patients (27.5%). Most T2DM patients presented with hematological parameters within normal limits. However, specific hematological disturbances were still identified, notably reductions in Hemoglobin (Hb) and Mean Corpuscular Hemoglobin (MCH), alongside alterations in leukocyte differential counts. These anomalies may correlate with inflammatory complications or anemia prevalent in the T2DM population. Microscopic urinalysis revealed a high frequency of abnormalities across several parameters. Abnormal epithelial cells were observed in 26 patients (65%), aberrant erythrocytes in 28 patients (70%), and atypical leukocytes in 26 patients (65%). The most prevalent finding was abnormal urinary crystals, detected in 33 patients (82.5%). These results underscore the necessity for consistent renal health monitoring and proactive management in diabetic care. In conclusion, the hematological and urinalysis profiles of T2DM patients offer preliminary insights into their clinical status and the extent of associated complications, serving as valuable indicators for comprehensive patient management.
BIOINFORMATIC ANALYSIS OF SUBTILISIN-K2 FROM INDONESIAN MOROMI: EVALUATION OF ITS ANTITHROMBOTIC POTENTIAL FOR FUNCTIONAL FOOD APPLICATION Syahbanu, Fathma; Kurniasari, Ratih; Riski Sefrina, Linda; Kurnianto, Muhammad Alfid
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 2 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.13669

Abstract

Microbial fibrinolytic enzymes are commonly found in various fermented foods of both plant and animal origin. While extensive studies have been conducted in Japan, Korea, and China, research in Indonesia remains limited despite its rich diversity of fermented foods. Moromi, an intermediate product of soy sauce fermentation, contains Subtilisin-K2, an enzyme proven in vitro to degrade fibrin and fibrinogen, indicating potential antithrombotic activity. This study investigated the antithrombotic properties of Subtilisin-K2 using bioinformatic approaches, including molecular docking (HADDOCK) and molecular dynamics (GROMACS). Subtilisin-K2 exhibited strong binding affinities with fibrin, fibrinogen, PAI-1, PAI-2, and α-antiplasmin, with Gibbs free energy values of –19.4, –15.6, –15.7, –18.2, and –13.3 kcal/mol, respectively. Molecular dynamics confirmed the stability of these complexes. These findings suggest that Subtilisin-K2 from Indonesian moromi exhibits significant bioactivity, underscoring the potential of Indonesian fermented products as valuable sources of functional enzymes, especially as antithrombotic potential.
SORUS MORPHOLOGY IN FERNS OF THE Polypodiaceae AND Tectariaceae FAMILIES IN THE ATO WATU WATERFALL AREA, AMONGGEDO DISTRICT Munir, Asmawati; Tenriawaru, Andi Besse; Sirniawan, Sirniawan
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 2 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.14180

Abstract

This study aims to determine the shape, color, and location of sori as well as the presence or absence of annulus in ferns (Pteridophyta) of the Polypodiaceae and Tectariaceae families in the Ato Watu Waterfall area, Amonggedo District, Konawe Regency, Southeast Sulawesi. The method used in this study was qualitative descriptive research with an exploratory method by exploring the Ato Watu Waterfall area to collect fern samples that had sori. The results of the study found six species of ferns in the Polypodiaceae family, including Drynaria quercifolia (L.), J, Lemmaphyllum accedens (Blume), Leptochilus henryi (Baker) X.C.Zhang, Microsorum membranifolium (R.Br.) Ching, Pyrrosia longifolia (Burm.fil.) C.V.Morton, Selliguea heterocarpa BI., FI. Jav (Mehltreter, et al., 2010: 835-838) And 5 species of ferns from the Tectriaceae family, including Tectaria angulata (Sw.) S. Chandra, Tectaria aurita (Sw.) S. Chandra, Tectaria barberi (Hook), Tectaria devaxa (Kze) Copel, Tectaria fuscipes (Wall. ex Bedd) C. Chr,. Of the 6 species of the Polypodiaceae family, 5 species of the Tectariaceae family found, they have different sorus characteristics, and each species has an annulus (Shin & Keun, 2017: 317-320).
THE VAPOR OF LEMONGRASS (Cymbopogon nardus) ESSENTIAL OIL FROM THE DIFFUSER AS MOSQUITO REPELLENT AND TRANQUILIZER Junedi, Sendy; Renanda, Salsa Billa Putri; Callista, Evelin Erlinda Elma; Nahak, Gabriella Ayu Chintya Velania
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 2 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.14193

Abstract

The COVID-19 pandemic and dengue fever present significant health challenges, especially in tropical regions such as Indonesia. This study aimed to explore the potential of citronella essential oil (Cymbopogon nardus) vapor, delivered via a diffuser, as both a tranquilizer and mosquito repellent. Citronella oil was isolated by water distillation and analyzed through GC-MS and TLC. The tranquilizing effect was evaluated using the Depression Anxiety and Stress Scale (DASS), while the mosquito repellent activity was tested against Aedes aegypti. Results showed that citronella oil contains key compounds such as citronellal, eugenol, cytronellol acetate and geraniol acetate. DASS scores decreased after exposure to citronella oil vapor, indicating its tranquilizing effect. In the mosquito repellent test, citronella oil caused a 68% mortality rate in Aedes aegypti after 60 minutes. These findings suggest that citronella essential oil vapor from diffusers is effective as both a tranquilizer and mosquito repellent.
THE ABUNDANCE OF ANTS (FORMICIDAE) IN COFFEE PLANTATIONS OF MERAPI, LAHAT DISTRICT Teristiandi, Novin; Yuliana, Meta
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 2 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.14455

Abstract

Ants are widely used as ecological indicators in agricultural landscapes, yet community responses can vary across local environmental gradients. This study assessed ground-active ant assemblages in coffee plantations in Merapi, Lahat (South Sumatra, Indonesia) at sites categorized as near versus far from a coal-mining area, using pitfall traps and community-level metrics. Mean temperatures during sampling were nearly identical between site categories (near 30.54 °C; far 30.56 °C). Ant assemblages showed contrasting patterns in richness and abundance: far sites contained six taxa but a lower total number of individuals (117), while near sites contained three taxa but higher total abundance (209). Species composition differed markedly, with far sites dominated by Solenopsis invicta and near sites dominated by Leptogenys sp. and Myopias emeryi. Diversity indices reflected these patterns: Shannon diversity was higher far from the mine (0.8906) than near (0.7215), while Simpson dominance was slightly higher far (0.5607) than near (0.5219), and evenness was higher near (0.6567) than far (0.4971). These findings indicate an association between distance class and ant community structure in coffee plantations, characterized by higher richness but stronger dominance at far sites and higher abundance but lower richness at near sites; however, conclusions about mining impacts should remain cautious given the observational design.

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