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Jurnal Bioteknologi & Biosains Indonesia
Published by Badan Riset dan Inovasi Nasional
ISSN : 24422606     EISSN : 2548611X     DOI : https://doi.org/10.55981/jbbi
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology
JBBI is published twice annually and provide scientific publication medium for researchers, engineers, practitioners, academicians, and observers in the field related to biotechnology and bioscience. This journal accepts original research papers, review articles, case studies, and short communications. The articles published are peer-reviewed by no less than two referees, and cover various biotechnology subjects related to the field of agriculture, industry, health, environment, bioinformatics, as well as life sciences in general. JBBI terbit dua kali setahun dan menyediakan media penerbitan ilmiah bagi para peneliti, insinyur, praktisi, akademisi, dan pemerhati di bidang yang terkait dengan bioteknologi dan biosains. Jurnal ini menerima naskah asli hasil penelitian, naskah ulasan, studi kasus, dan komunikasi singkat. Naskah yang diterbitkan adalah peer-review oleh tidak kurang dari dua orang penelaah, dan mencakup berbagai cabang bioteknologi yang terkait dengan bidang pertanian, industri, kesehatan, lingkungan, bioinformatika, serta ilmu kehidupan pada umumnya.
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 146 Documents
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MORPHOLOGICAL AND TEXTURAL FEATURE EXTRACTIONS FROM FUNGI IMAGES FOR DEVELOPMENT OF AUTOMATED MORPHOLOGY-BASED FUNGI IDENTIFICATION SYSTEM Pramesti, R. Putri Ayu
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 9 No. 2 (2022)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2022.1759

Abstract

Due to widely varied microscopic shapes, fungal classification can be performed based on their morphological features. In morphology-based identification process, feature extraction takes an important role to characterize each fungal type. Previous studies used feature extraction of fungal images to detect the presence of fungal. In this study, morphological and textural features were extracted to classify three types of fungi: Aspergillus, Cladosporium and Trichoderma. Geometry and moment were used as morphological features. To perform textural feature extraction, the local binary pattern (LBP) and gray level co-occurrence matrix (GLCM) feature extraction method were used. We compared the implemented feature extraction methods in order to get the best classification result. The result showed that geometrical features has the accuracy of 65%, higher than that of LBP (60%), GLCM (45%), and moment accuracy (55%). This suggested that geometric features is important for fungal classification based on their morphology.
EFFECTS OF SEED COATING USING PLANT-BENEFICIAL MICROBES ON THE GERMINATION OF Centrosema pubescens SEEDS Handayani, Indri
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 9 No. 2 (2022)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2022.1769

Abstract

Centrosema pubescens is a legume plant that is commonly used as animal feed, cover crop, and one of the plants used to reclaim critical land. This study was conducted to increase the germination of C. pubescens seeds by coating the seeds using a coating material enriched with a consortium of microbes consisting of nitrogen-fixing bacteria, phytohormones-producing bacteria, phosphate-solubilizing bacteria, and arbuscular mycorrhizal fungi. Germination test on control, seed coating, and microbial seed coating was carried out using the top of the sand method for 28 days. Observational data were tested using ANOVA statistics and LSD tests. Results of the research showed the moisture content of the seeds was 12.45%, the purity of the seeds was 95.11% and the weight of 1000 seeds was 23.74 g. The germination test denoted that the number of normal germinated seeds in seed coating and microbial seed coating was significantly different from the control. In addition, dead seeds in microbial seed coating treatment had the lowest value and were significantly different from other treatments. These results indicate that microbial seed coating can increase seed germination and considerably reduce seed death due to seed-borne pathogens.
PRODUCTION OF HUMAN INSULIN PRECURSOR IN A 10 LITER BIOREACTOR USING A METHANOL PULSE FEEDING Puspitasari, Dian Japany
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 9 No. 2 (2022)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2022.1770

Abstract

Indonesia as a country that had 19.5 million diabetic patients in 2021 is still importing raw materials of human insulin. The development of human insulin production process is needed to support the government’s objective to be independent in medicine aspect. Human insulin precursor (HIP) expressed from Pichia pastoris X33/pD902-IP had been developed and optimized in small-scale cultivation. However, the scaling up in bioreactor 10 L has not been studied. Using a 10 L-bioreactor the fermentation of P. pastoris X33/pD902-IP was developed. Fermentation was done in 120 hours using a basal salt medium (half concentration) for the vegetative and induction media. To induce HIP expression, methanol is fed by pulse strategy with a gradient concentration of 1-3% (v/v) for 48 hours. The dry cell weight (DCW) and HIP titers were 72 g/L and 286 mg/L, respectively. This development is the first fermentation of HIP expressed by P. pastoris X33/pD902-IP in a 10 L-bioreactor.
VOLATILE COMPOUND ANALYSIS OF AROMATIC RICE MUTANT LINES USING HS-SPME/GC-MS Riadi, Muh
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 9 No. 2 (2022)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2022.1771

Abstract

Volatile compound is one of the key factors for aromatic components of rice. This study aimed to identify the key aroma components and their relationship with plant productivity in the Pare Bau variety mutant lines and its wild type. Volatile extraction was carried out using the headspace solid-phase microextraction (HS-SPME) method and analysed by the Gas Chromatography-Mass Spectroscopy (GC-MS) instrument. The results of the identification of volatile compounds showed a total of 224 compounds in the mutant lines and wild type. However, only 14 compounds were suspected as key aroma compounds in Pare Bau rice, namely 2-Acetyl-1-pyrroline, Indole, 1-Octanol, 1-Octen-3-ol, 2,4-Nonadienal, (E,E)-, Octanal, 2-Nonenal, (E)-, 2-Octenal, (E)-, Decanal, Hexanal, Nonanal, Furan, 2-pentyl-, toluene and vanillin. The results on aroma compounds of mutant lines using principal component analysis showed that there were differences in the main characteristics of several strains and wild type. There was no relationship between increasing volatile concentration and plant productivity, vice versa.
UTILIZATION OF SAGO DREGS AS RUMINANT FEED BY USING THE FERMENTATION METHOD: LITERATURE REVIEW Susanti, Titi Lahanda
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 9 No. 2 (2022)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2022.1772

Abstract

Every year there is a change in the stock of ruminant feed that occurs in the rainy season and water shortages in the dry season. Utilization of agricultural waste as an alternative feed is one way to overcome these problems. One of the wastes that have the potential to be used as feed ingredients is sago waste. Sago pulp is a waste that is rich in lignocellulose, namely cellulose. Several biotechnology applications in ruminant feed fermentation can improve properties such as taste, aroma, shelf life, texture and nutritional value of food. Fermentation using mold or yeast, as well as bacteria or a mixture of various microorganisms can increase the nutrients in the feed needed by ruminant feed. Processing of lignocellulosic materials is required to obtain optimal degradation results. The degradation process will convert lignocellulosic material into raw materials that are easily digested by the ruminant. Enzymes produced by microorganisms can increase crude protein, crude fat, carbohydrates, crude fiber, vitamins and minerals. Thus, the fermentation method of sago dregs and its use as feed can increase the nutritional value, so that productivity can be increased when given as feed.
REVIEW: ANIMAL SERUM REPLACEMENT IN MESENCHYMAL STEM CELLS CULTURE Noviantari, Ariyani
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 9 No. 2 (2022)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2022.1773

Abstract

Mesenchymal stem cells (MSCs) are being used in clinical applications and must comply with Good Manufacturing Practices (GMP) standards and The National Agency of Drug and Food Control (NA-DFC)) regulations. MSCs cultured using a culture medium and added with several supplements like animal serums. However, animal serums can be a source of virus transmission. Therefore, it is necessary to substitute supplements for the animal serum that are safe to use in cell therapy using MSCs. The paper discusses substituting animal serum MSCs culture. This paper is a literature review through a literature search in scientific journals and research reports that explains the various studies on free serum in the culture of MSCs. It can be concluded that human platelet lysate (hPL), human platelet-rich plasma (hPRP), human serum (hS), human umbilical cord serum/plasma (hUCS/P), or human plasma-derived supplement for cell culture medium (SCC) can be used as substitutes for animal serum in MSCs culture.
INCREASING RECOMBINANT PENICILLIN G ACYLASE PRODUCTION: GENETIC, PROTEIN ENGINEERING, AND PRODUCTIVITY IMPROVEMENT Helianti, Is
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 9 No. 2 (2022)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2022.1775

Abstract

B-lactam derived antibiotics are the most used globally for treatment against different infections caused by pathogenic bacteria and comprises 65% of the world antibiotics. Recently, penicillin G acylase (PGA) is used as biocatalyst for those B-lactam antibiotics production by which 6-aminopenicillanic acid (6-APA) or 7-aminodeacetoxycephalosporanic acid (7-ADCA) as the building blocks is produced. Commercialized PGA from native microbial resources are still limited to E. coli. Therefore, genetic engineering approach such as cloning and expression in other microbial hosts were assessed to enhance bacterial strains that produce PGA. However, such improvement could increase immature precursors accumulation and lowering the enzyme yield, activity, or stability. This review focus on the review of PGA recombinant produced by several microbial host, their expression levels, and improvement achieved by some modification such as replacement of signal peptide and promoter continued to protein engineering to utilize the enzymes in synthetizing amoxicillin rather than to hydrolyses Penicillin G.
INHIBITORY ACTIVITY OF Trichoderma harzianum AGAINST PUTATIVELY PATHOGENIC FUNGUS ON RODENT TUBER (Typhonium flagelliforme) PLANT Sriherwanto, Catur
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 9 No. 1 (2022)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2022.1781

Abstract

Trichoderma spp. are globally considered as the most dominant biofungicide in the market. Reports on Trichoderma spp. efficacy against pathogenic fungi in commercial crops have been numerous, but much less in medicinal plants. This study aimed at testing the potential biofungicidal activity of Trichoderma harzianum in inhibiting the growth of a putatively pathogenic fungus isolated from rodent tuber (Typhonium flagelliforme) plant. The methods consisted of isolation of fungi from the plant’s surface, soil, and polybags. The isolates were then screened for their putative pathogenicity against rodent tuber before being subjected to 16S rRNA molecular identification and in vitro antagonist test using T. harzianum. Result showed that only isolate K4 showed pathogenicity on T. flagelliforme, and was molecularly identified as Lasiodiplodia theobromae, known globally as fungal pathogen attacking various plants. L. theobromae was inhibited by T. harzianum with inhibition index of 23.0 ± 4.3%, which was about twice higher than that of the positive control nystatin 100.000 IU mL (11.1 ± 0.6%). In conclusion, T. harzianum inhibited the growth of L. theobromae in vitro, hence indicating its biofungicidal potential.
ETHANOL EXTRACT OF Moringa oleifera INCREASED THE NUMBER OF SPERMATOZOA AND IMPROVED SPERM MORPHOLOGY OF OLD Rattus norvegicus Widiastini, Luh Putu
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 9 No. 1 (2022)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2022.1782

Abstract

Aging in men can lead to decreased semen volume, abnormal sperm morphology, and decreased sperm motility. This study aims to determine the effect of ethanol extract of moringa (Moringa oleifera) leaf on the number and morphology of spermatozoa in old Wistar rats (Rattus norvegicus). This study used healthy old rats, 18–19 months old, with a bodyweight of 200–250 g and no physical disabilities. The 36 rats were divided into 2 groups, i.e. the treatment group (fed with ethanol extract of moringa leaves 50 mg/kgBW/0.5 mL CMC 0.5% every day) and the control group (0.5 mL CMC 0.5% every day) for 30 days. The results showed that moringa leaf ethanol extract increased the number of spermatozoa (p-value 0.000) and improved spermatozoa morphology (p-value 0.000). It can be concluded that the ethanol extract of moringa leaves significantly increased the number and improved the morphology of the spermatozoa in the aged rats.
SEGREGATION ANALYSIS OF MORPHOLOGICAL CHARACTER AND BIOACTIVE GENOTYPE OF RICE PLANT F3 (CEMPO SALAMET/IR64) POPULATION Ubaidillah, Mohammad
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 9 No. 1 (2022)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2022.1783

Abstract

Red rice contains high anthocyanin and bioactive antioxidant compounds that prevent free radical reactions. Cempo Salamet has potential as an antioxidant source, and the characteristics are red colored grains, 4–5 months old, 169 cm plant height, 7 productive tillers per plant, and resistance to blast disease. IR64 had been developed with the following characteristics: 3 months old, 85 cm plant height, 20–35 productive tillers per plant, resistance to brown leafhoppers pigment. This study aimed to obtain information on the segregation of the F3 population from crosses between the Cempo Salamet and IR64 varieties. Research methods included preparation and maintenance with genotype analysis. PCR analysis was conducted using SSR markers with primer RM346, RM316, RM228, and RM339. The segregation in F3 plants was 50% for >130 cm plant height, 51% for 10–19 tillers per plant, 67% for 2.2 g/100-grain weight, and 33% strong red for colour intensity. The findings demonstrated that SSR markers RM346, RM339, and RM228 could validate Cempo Salamet, IR64, and F3 DNA bands. However, RM316 could not validate all DNA bands in the research sample.

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