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Jurnal Bioteknologi & Biosains Indonesia
Published by Badan Riset dan Inovasi Nasional
ISSN : 24422606     EISSN : 2548611X     DOI : https://doi.org/10.55981/jbbi
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology
JBBI is published twice annually and provide scientific publication medium for researchers, engineers, practitioners, academicians, and observers in the field related to biotechnology and bioscience. This journal accepts original research papers, review articles, case studies, and short communications. The articles published are peer-reviewed by no less than two referees, and cover various biotechnology subjects related to the field of agriculture, industry, health, environment, bioinformatics, as well as life sciences in general. JBBI terbit dua kali setahun dan menyediakan media penerbitan ilmiah bagi para peneliti, insinyur, praktisi, akademisi, dan pemerhati di bidang yang terkait dengan bioteknologi dan biosains. Jurnal ini menerima naskah asli hasil penelitian, naskah ulasan, studi kasus, dan komunikasi singkat. Naskah yang diterbitkan adalah peer-review oleh tidak kurang dari dua orang penelaah, dan mencakup berbagai cabang bioteknologi yang terkait dengan bidang pertanian, industri, kesehatan, lingkungan, bioinformatika, serta ilmu kehidupan pada umumnya.
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 146 Documents
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THE APPLICATION OF Fe AND Cr(III) IN GROWING MEDIA AND ITS EFFECT ON PLANT GROWTH AND Cr(III) OXIDATION ON Tagetes erecta Kasmiyati, Sri
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 9 No. 1 (2022)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2022.1784

Abstract

The oxidation of Cr(III) to Cr(VI) in the environment has a detrimental impact because it can change the form of non-toxic Cr(III) to Cr(VI), which is toxic to organisms. The study aimed to examine the effect of the application of iron (Fe) and trivalent chromium (Cr(III)) compounds in Tagetes erecta growing media on growth and Cr(III) oxidation. Concentrations of Cr(III) 0, 100, and 500 mg/L and Fe 0, 3, 15, and 30 mg/L were applied to the growing media of T. erecta as the model plant. The growth and accumulation of Cr(VI) in plants were measured to determine the effect of Fe and Cr(III) treatment on growth and Cr(III) oxidation. The accumulation of Cr(VI) in the roots and shoots of T. erecta increased significantly due to the addition of Fe in the growing media treated with Cr(III). The highest accumulation of Cr(VI) in the roots and shoots of T. erecta found in the treatment of Cr(III) 500 mg/L and Fe 30 mg/L, were respectively 0.092 g/L and 0.070 g/L. The addition of Fe in growing media containing Cr(III) increased plant height, root length, and shoot dry weight but decreased leaf number and root dry weight. T. erecta root biomass was more affected by the toxic impact of Fe than Cr(III). On the other hand, the inhibition of leaf formation was caused by the toxic effect of Cr(III) rather than Fe.
MITOCHONDRIAL DNA DIVERSITY IN FOUR POPULATIONS OF INDONESIAN FRESHWATER GIANT PRAWN (Macrobrachium rosenbergii) Aliah, Ratu Siti
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 9 No. 1 (2022)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2022.1785

Abstract

Mitochondrial DNA has been widely applied to analyze fish genetic diversity, especially the cytochrome oxidase subunit I (COI) gene, which can be used as a marker of typical variation patterns, both intraspecific and interspecific crossbreeding. Haplotype diversity was observed in four populations of giant freshwater prawns (Macrobrachium rosenbergii) originating from Peureulak River in Aceh, Tabuk River in South Kalimantan, Bengawan Solo River in East Java, and the Siratu strain. Haplotype diversity was observed in the mitochondrial DNA COI gene of 1516 bp and restricted using 5 enzymes, namely AvaII, MspI, RsaI, HaeIII, and TaqI. The diversity of mtDNA COI haplotypes in the population of Peureulak River, Tabuk River, Bengawan Solo River, and Siratu were 0.8183, 0.6222, 0.7461, and 0.8044, respectively. The dendrogram of genetic distance showed that the Bengawan Solo River populations and the Siratu strain were in the same cluster, while Peureulak River and Tabuk River populations were in another group. The four populations can be used as genetic sources in crossbreeding activities based on haplotype data for each population, haplotype diversity, and genetic distance.
RESPONSE OF SEED GERMINATION AND GROWTH OF Nepenthes gymnamphora Nees IN VITRO TO THE CONCENTRATION OF MS MINERAL SALT, PEPTONE AND THIDIAZURON Nurchayati, Yulita
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 9 No. 1 (2022)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2022.1786

Abstract

Nepenthes gymnamphora Nees is a Java's rare endemic species. Ex-situ conservation of this endangered species can be done through in vitro culture technique. The aims of this study were to determine (1) the mineral salt concentration of MS basal media and addition of peptone (P) on N. gymnamphora seed germination and seedling emergence and (2) the effects of TDZ in ½MS medium on seedling growth. Seeds were surface sterilized and cultured on four media formulations (½MS, MS, ½MS+P, MS+P) for 8 weeks. In the second experiment, ten-week-old seedlings, 0,25 cm in length were cultured on ½MS supplemented with 0, 0,5, 1,0, or 1,5 mg/L TDZ. Seedling growth was recorded at 8 weeks of culture. Results of this experiment showed that ½MS was the best medium for N. gymnamphora seed germination as indicated by the highest percentage of germination, the tallest seedling, and the fastest seedling emergence. Moreover, the best growth of N. gymnamphora was found on ½MS without TDZ.
MOLECULAR IDENTIFICATION AND MORPHOLOGICAL CHARACTERIZATION OF PATCHOULI (Pogostemon sp.) FROM BATANG REGENCY, CENTRAL JAVA PROVINCE Kusumaningrum, Hermin Pancasakti
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 9 No. 1 (2022)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2022.1787

Abstract

Patchouli (Pogostemon cablin Benth.) is an essential oil-producing plant from Batang Regency that has excellence in patchouli alcohol contents and highly survives in any condition. Molecular identification has been done in the ITS region because DNA sequences in the ITS rRNA region evolved faster than in other areas. This study aimed to get the molecular and morphological identity of patchouli (Pogostemon sp.) from BPP Kabupaten Batang. The study consisted of sample preparation, DNA isolation, amplification, electrophoresis, sequence data analysis, and phylogenetic analysis using MEGA X. All parts of plant were morphologically identified and compared with patchouli from Sidikalang, Aceh, Java, and China. Extraction DNA produced 301.7 ng ?L–1 concentration and 1.93 purity. Amplification of ITS fragment patchouli produced a 670 bp-sized single band. Phylogenetic analysis showed patchouli BPP related to Pogostemon cablin (KR608752.1) with 98% coverage identity. BPP patchouli showed 62,5% morphological similarity with Sidikalang patchouli compared to Java patchouli. In conclusion, BPP patchouli is a Sidakalang patchouli P. cablin that has undergone environmental adaptation.
GENETIC MUTATION INDUCTION OF Monstera adansonii ON VARIOUS MUTAGENS BY DRIPS APPLICATION Haswin, Dwi Wahyuni
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 9 No. 1 (2022)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2022.1788

Abstract

Monstera adansonii has wide leaves with a split pattern on its strands (split leaf), smooth and shiny to create a basic visualization (background filler) in flower arrangements. This study aims to obtain genetic changes using chemical mutagens. Chemical mutagens used were 250 ppm EMS, 400 and 800 ppm streptomycin, 400 and 800 ppm GA3. The observations were performed after treatments on the parameter of amount of chlorophyll, stomata and leaf color changes. The results showed that both streptomycin treatments significantly reduced the plant height and the number of leaves. The application of streptomycin in high concentration (800 ppm) decreased the total amount of chlorophyll content in the leaves and changed the color as well as the shape of the Monstera leaves. All chemical mutagen treatments had no effect on stomatal density and stomatal area. The changes in leaf color and shape occurred in the high concentration of 800 ppm streptomycin treatment, could not detected by SSR marker.
ANTIBACTERIAL ACTIVITY PROFILE OF MANGROVE ENDOPHYTIC FUNGI ISOLATED FROM BERAU REGENCY, INDONESIA Sukarno
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 9 No. 1 (2022)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2022.1789

Abstract

Mangrove endophytic fungi have the potential to produce secondary metabolites with antibacterial properties. This study aimed to obtain endophytic mold isolates associated with mangrove plants and their antimicrobial activity. Seventeen endophytic molds were isolated from 7 mangrove species originating from Berau, East Kalimantan, Indonesia. Three extracts from the mold isolates OJ-B, OJ-Bu, and 6Xg-Bh showed the highest antibacterial activity with an inhibition zone of 9.63–25.4 mm and with MIC (minimum inhibitory concentration) and MBC (minimum bactericidal concentration) values ??of around 0.625–5 and 2.5–10 mg mL–1, respectively. LC-MS (liquid chromatography-mass spectrometry) analysis identified several compounds with antibacterial potential, namely beauvericin, choline, nicotinic acid, pyridoxine, quinoline, cyclo (phenylalanyl-prolyl), fusarin c, oleamide and borrelidin. The 3 species were molecularly identified as Diaporthe phaseolorum, Fusarium proliferatum and Phomopsis sp. Thus, the endophytic molds from several of the mangrove species produced bioactive compounds as antibacterial.
ANALYSIS OF BBM, LEC, AND SERK EXPRESSIONS IN CALLUS OF SUGARCANE (Saccharum officinarum L.) AT SOMATIC EMBRYOGENESIS DEVELOPMENT STAGES Dewanti, Parawita
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 9 No. 1 (2022)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2022.1790

Abstract

Callus formed in somatic embryogenesis is divided into two types, embryogenic and non-embryogenic callus. Embryogenic callus can be distinguished by the expression of genes as markers related to somatic embryogenesis which are expected to be used as markers to detect callus that has embryogenic capabilities in sugarcane. The aim of this study was to determine the expressions of somatic embryogenesis-related genes in sugarcane. The genes analysis was carried out using somatic embryogenesis callus and using a kit for RNA analysis. Results showed that there were expressions of Baby Boom (BBM), Leafy Cotyledon (LEC), dan Somatic Embryogenesis Receptor Like-Kinase (SERK) gene with specific primer of those three genes, which were collected from embryogenic callus in mass, globular, scutelar, and coleoptilar pre-embryo stages, meanwhile there were no expressions of those genes collected from non-embryogenic callus. Amplification result from PCR product of cDNA using three gene primers detected were in 500 bp for BBM, 400 bp for LEC, and 700 bp for SERK gene.
OPTIMIZATION OF Agrobacterium-MEDIATED GENETIC TRANSFORMATION OF oshox4 GENE IN SATOIMO TARO (Colocasia esculenta var. antiquorum) Hartati, N. Sri
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 9 No. 1 (2022)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2022.1791

Abstract

Satoimo (Colocasia esculenta var. antiquorum) can be considered as an alternative food to support food diversification. The aim of this research was to obtain an optimum condition of the genetic transformation method of Satoimo taro through Agrobacterium-mediated genetic transformation using binary vector harboring gene construct of oshox4 and hpt gene as a selectable marker to produce transgenic plants. Plant materials that were used in this study were meristem, root, leaf, petiole, and basal stem. Transformation procedures were carried out using three different co-cultivation periods (1, 2, and 3 days) and different optical densities of A. tumefaciens cells (0,5 and 0,7). Furthermore, callus induction was performed on MS medium containing 2.4-D, cefotaxime, hygromycin and then regenerated on MS medium containing thidiazuron and hygromycin. The putative transgenic plantlets were selected on MS medium containing hygromycin and analyzed using PCR. The results showed that putative transgenic plants derived from the basal stem could survive on a selection medium containing hygromycin and based on PCR analysis some of them contained the oshox4 and hpt genes.
ANTIDIABETIC ACTIVITY OF LEAF EXTRACT OF Clerodenrum fragrans Vent Willd IN Rattus novergicus INDUCED BY ALLOXAN Silaban, Saronom
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 9 No. 1 (2022)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2022.1792

Abstract

Sarang banua plants are grown in Simalungun, North Sumatra, Indonesia, and have been used by the community as traditional medicinal plants. Sarang banua plant is a type of Clerodendrum fragrans Vent Willd, including the family Verbenaceae. This study aimed to determine the antidiabetic activity of the leaf extract of sarang banua (C. fragrans Vent Willd) in white male rats (Rattus novergicus) induced by alloxan. This study used a RAL design with seven treatments, namely (K0) standard feed, (K1) Na-CMC 0.5%), (K2) metformin, (K3) ethanol extract 100 mg/kg bw, 200 mg/kg bw (K4), 300 mg/kg bw (5), (K6) ethyl acetate extract 200 mg/kg bw and 300 mg/kg bw (K7). Groups K1 to K7 were induced by alloxan before being given treatment. Each treatment was replicated three times. The results showed that the application of leaf extract of the C. fragrans affected on reducing the blood glucose levels of alloxan-induced rats. The used of ethanolic extract of C. fragrans 100 mg/kg bw resulted in the highest percentage decrease in blood glucose (54.46 ± 5.60%) of hyperglycemic rats induced by alloxan, close to a positive control (56.63 ± 1.86%).
MICROBIAL BIOCONVERSION TO PRODUCE NUTRACEUTICAL AND PHARMACEUTICAL BIOACTIVE COMPOUNDS Mahyuni, Siti
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 9 No. 1 (2022)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2022.1793

Abstract

he bioconversions of bioactive health supporting compounds using certain microorganisms have long been known, however it has not been widely applied in industrial scale to produce nutraceutical and products of high economic value. This review article will discuss the group of steroids, carotenoids and statins which were produced by microbial bioconversion using the various substrates and conditions. The information presented in this article were collected from scientific books and articles in national and international journals which can be validated and traced back to their sources through many sites such as google.com, google scholar, NCBI and Science Direct. From the information compiled in this article, it can be concluded that the bioconversion of bioactive compounds by microorganisms has shown positive results and potential to be further optimized and applied in an industrial scale to produce natural bioactive compounds as basic ingredients for nutraceutical and pharmaceutical products with high economic value.

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