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Jurnal Bioteknologi & Biosains Indonesia
Published by Badan Riset dan Inovasi Nasional
ISSN : 24422606     EISSN : 2548611X     DOI : https://doi.org/10.55981/jbbi
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology
JBBI is published twice annually and provide scientific publication medium for researchers, engineers, practitioners, academicians, and observers in the field related to biotechnology and bioscience. This journal accepts original research papers, review articles, case studies, and short communications. The articles published are peer-reviewed by no less than two referees, and cover various biotechnology subjects related to the field of agriculture, industry, health, environment, bioinformatics, as well as life sciences in general. JBBI terbit dua kali setahun dan menyediakan media penerbitan ilmiah bagi para peneliti, insinyur, praktisi, akademisi, dan pemerhati di bidang yang terkait dengan bioteknologi dan biosains. Jurnal ini menerima naskah asli hasil penelitian, naskah ulasan, studi kasus, dan komunikasi singkat. Naskah yang diterbitkan adalah peer-review oleh tidak kurang dari dua orang penelaah, dan mencakup berbagai cabang bioteknologi yang terkait dengan bidang pertanian, industri, kesehatan, lingkungan, bioinformatika, serta ilmu kehidupan pada umumnya.
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 146 Documents
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LIPID PRODUCTION FROM BR. 2.2 OLEAGINOUS FUNGAL ISOLATE USING ACETATE, GLYCEROL, AND MOLASSES AS CARBON SOURCES Alifiyatin, Chikmatul; Darmayanti, Salma; Firdasi, Wanny; Ilmi, Miftahul
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 10 No. 1 (2023)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2023.1745

Abstract

Microorganisms that accumulate more than 20% of their dry cell weight as lipids are called oleaginous microorganisms. Oleaginous microorganisms can grow well on various carbon sources other than glucose. These non-glucose alternative carbon sources could potentially reduce high biofuel manufacturing costs. BR. 2.2 isolate is an oleaginous fungus that accumulates 0.62 g L-1 lipids using glucose as a carbon source. This study aims to determine the effect of acetate, glycerol, molasses, and C/N ratios on lipid accumulation of the BR.2.2 isolate. The highest lipid produced by the BR. 2.2 isolate using acetate is 0.196 g L-1 at a C/N ratio of 400, 0.229 g L-1 at a C/N ratio of 225 using glycerol, and 1.97 g L-1 at a C/N ratio of 25 using molasses in 144 hours of incubation. The results revealed that the accumulation of lipids increased with the rising acetate and glycerol C/ N ratios and incubation period. Meanwhile, the accumulation of lipids decreased with increasing molasses C/N ratio.
SCREENING AND CHARACTERIZATION OF SOIL FUNGAL ISOLATES FOR INHIBITING THE GROWTH OF Ganoderma boninense Haqqa, Zhafira Amila; Rupaedah, Bedah; Handayani, Indri; Wahid, Abdul; Sugianto, Mahmud
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 10 No. 1 (2023)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2023.1747

Abstract

Ganoderma boninense, a fungus recognized as a causative agent of basal stem rot and upper stem rot, is primarily found in oil palm plantations (Elaeis guineensis Jack.). This study aimed to identify soil fungal isolates with the greatest potential for inhibiting the pathogenic fungus G. boninense. The research employed curative antagonist testing using in vitro dual culture. Fungal isolates demonstrating the highest inhibition percentages were characterized through macroscopic and microscopic observation, and their hemolysis properties were assessed using blood agar media. Soil fungal isolates FA 3.8 and FA 2.8 exhibited the highest inhibition percentages, reaching 91% and 88%, respectively. Based on morphological characterization at both macroscopic and microscopic levels, FA 3.8 displayed similarities to Trichoderma, while FA 2.8 exhibited similarities to Penicillium. Hemolysis testing results on blood agar media indicated that both isolates exhibited gamma hemolysis or non-hemolysis, as they lacked red blood lysis properties.
OPTIMIZATION OF ENZYME-MICROWAVE ASSISTED EXTRACTION, CHARACTERIZATION AND ANTIOXIDANT ACTIVITY OF POLYSACCHARIDE FROM Ganoderma lucidum Listriyani, Lira Windriawati; Hudiyono, Sumi; Rudiyono, Rudiyono; Zulaeha, Siti; Wibisana, Ahmad
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 10 No. 1 (2023)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2023.1748

Abstract

Enzyme-Microwave Assisted Extraction (EMAE) is a new process for extracting Ganoderma lucidum polysaccharides (GLPs). Cellic® CTec2 was chosen as an enzyme that assists in microwave extraction. The four variables involved in this study were enzyme concentration (%), enzymatic reaction time (minutes), solvent-to-solid ratio (mL/g), and microwave extraction time (minutes). This study showed that the enzyme concentration and solvent-to-solid ratio had a significant effect on the response in the range studied. Yield extraction of polysaccharides from experiments conducted at optimum conditions showed good agreement with the predictions from the model. The EMAE method showed a higher polysaccharide extraction yield than hot water extraction (HWE) method. GLPs from EMAE method had antioxidant activity of 79.47 ± 0.71% (DPPH) and 0.884 ± 0.013 mM Fe2+/L (FRAP), where these values were higher than those of the HWE method.
ANTIOXIDANT EFFECT OF MORINGA (Moringa oleifera) LEAF ETHANOL EXTRACT ON SPERMATOGENESIS IN OLD WISTAR RATS (Rattus norvegicus) Widiastini, Luh Putu
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 9 No. 2 (2022)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2022.1749

Abstract

Infertility is the failure of pregnancy after regular sexual intercourse for 6-12 months without contraception. Infertility in man is primarily caused by damaged sperm production, for example, impairments in the spermatogenesis process, low spermatozoa concentrations, morphological factors, and abnormal sperm motility. The purpose of this study is to ascertain the antioxidant effect of moringa (Moringa oleifera) leaves ethanol extract on spermatogenesis (spermatogonia, spermatocytes, and spermatids counts) in old Wistar rats (Rattus norvegicus). This study took healthy old Wistar rats aged 18-19 months with a body weight of 200-250 g and with no physical disabilities. A total of 36 was Wistar rats divided into two groups, namely the treatment group (supplied with Moringa leaf ethanol extract of 50 mg/kgBW/ and 0.5% Carboxymethyl cellulose (CMC) of 0.5 mL per day) and the control group (only with 0.5% CMC of 0.5 mL per day) for 30 days. The results showed that there was a significant difference in the spermatogonia, spermatocyte, and spermatid counts between the group treated with Moringa leaf ethanol extract and the control group, resulting in a p-value of 0.000. Therefore, it can be concluded that Moringa leaf ethanol extract had a significant influence on the spermatogonia, spermatocyte, and spermatid counts in old Wistar rats.
MICROPROPAGATION OF POTATO (Solanum tuberosum L.) cv. GRANOLA IN LIQUID MEDIUM USING AERATION SYSTEM FOR G0 SEED PRODUCTION Karyanti
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 9 No. 2 (2022)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2022.1751

Abstract

Disease-free potato seeds of high quality can be obtained via in vitro culture. The use of liquid medium during in vitro cultures might boost the number of plantlets produced, however, the problem of hyperhydricity in plantlets was often encountered. This study aimed to investigate effects of different sucrose concentrations and application of aeration system on micropropagation of potato cv. Granola using liquid medium. Aseptic nodal explants with 3-4 nodes from established in vitro cultures were subjected to MS liquid medium with a factorial treatment of three sucrose concentrations (0, 7.5, 15 g. L-1) and two culture (with and without aeration). The results showed that MS medium with 7.5 g L-1 sucrose was the best medium to produce the highest number of shoots and nodes. Furthermore, it was found that application of aeration system in MS liquid culture decreased plantlet hyperhydricity and increased the number of shoots, number nodes, plantlet height, as well as improved plantlet morphology and vigor. Application of the aeration system in liquid medium produced 200-230 new potato plants per bioreactor in the acclimatization stage and an average of 2773.5 G0 mini tubers.
APPLICATION OF RECOMBINANT TRIACYLGLYCEROL LIPASE AND CARBOXYLESTERASE ENZYMES FROM Bacillus velezensis STRAIN S3 FOR POLYESTER SURFACE MODIFICATION Helianti, Is
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 9 No. 2 (2022)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2022.1753

Abstract

Enzymatic polyester surface modification can be performed with lipase and esterase enzymes. In this study, the polyester fabric modification utilized triacylglycerol lipase (TGA) and carboxylesterase (CES) recombinant enzymes. The effect of these treatments was observed by determining the hydrophilicity level, dye absorption level, hydroxyl group measurement, and fiber surface morphology. The results revealed an elevated hydrophilicity level in polyester fabric, followed by dye absorption improvement and carboxyl group increase. The water absorption times required by the fabric based on the results of TGA, CES, comparative lipase, and negative control treatments were 3±0.05 seconds, 3.5±0.07 seconds, 5±0.05 seconds, and 80±11.54 minutes, respectively. Dye absorption test in polyester fabric based on these groups mentioned above were 52±0.5, 58±0.5, 178±0.5, and 2968±290 seconds. The total hydroxyl group measurement in polyester fabric was observed at 30.9±0.09, 30.5±0.05, 28.6±0.09, and 3 meq/100 g. The SEM observation showed that the enzymatic hydrolysis could alter the porous structure and surface of the fibers.
TOTAL LIGNIN CONTENT AND AGROMORPHOLOGICAL CHARACTER DIVERSITIES OF 30 INDONESIAN RICE (Oryza sativa L.) ACCESSIONS Nugroho, Satya
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 9 No. 2 (2022)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2022.1754

Abstract

Lignin is one of lignocellulosic components in vascular plants, essential in plant mechanical properties, water transport, and defense against pathogens. Furthemore, lignin has been applied in various industry. This study aimed to explore variation of lignin content and its-related morphological traits of Indonesian rice accessions, providing beneficial information for breeding approaches to improve utilization characteristics of grass biomass. Therefore, the total lignin contents of thirty Indonesian rice accessions using Thioglycolic Acid Lignin (TGAL) method and their correlation to seven agromorphological characters using Pearson correlation analysis were investigated. Variation of lignin content ranged from 8.38 to 20.75% (of cell wal residue, CWR), and the average value was 13.55%. Correlation analysis showed that lignin total positively correlated with plant height, stem length, panicle length, stem diameter, total fresh weight, and panicle weight per tiller. On the other hand, the number of tillers had a significantly negative correlation to lignin contents.
OPTIMIZATION OF Bacillus paramycoides FERMENTATION MEDIUM TO INCREASE THE PRODUCTION OF 5-AMINOLEVULINIC ACID IN A 10 LITER FERMENTER Monconegoro, Dicky Adihayyu
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 9 No. 2 (2022)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2022.1755

Abstract

5-Aminolevulinic acid is an essential precursor for the biosynthesis of tetrapyrrole compounds, such as chlorophyll and heme. 5-ALA has the potential to be used as a plant growth and antioxidant activity enhancer. 5-ALA can be produced through fermentation by Bacillus paramycoides. This study aimed to optimize B. paramycoides fermentation medium to increase 5-ALA production. The optimization was carried out using response surface method (RSM) experimental design. 5-ALA production in a 10 L fermenter was conducted using an optimized medium and supplemented with MSG as a precursor and wood vinegar as an inhibitor. The results showed that the best medium composition was 27.78 g L-1 molasses; 9.145 g L-1 urea; 8.838 g L-1 NaCl; and 32.07 g L-1 glucose, resulting in 10.749 (log CFU mL-1) and 255.30 µM 5-ALA. 5-ALA production trial in a 10 L fermenter produced 581.82 µM 5-ALA. Medium optimization and precursor-inhibitors addition in the fermentation increased the 5-ALA yield 3.2 times compared to before optimization.
ANTIBACTERIAL ACTIVITY OF Chaetoceros calcitrans AGAINST PATHOGEN Staphylococcus aureus AND Staphylococcus epidermidis CAUSING SKIN INFECTION Naully, Patricia Gita
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 9 No. 2 (2022)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2022.1757

Abstract

The microalgae Chaetoceros calcitrans has potential as a natural antibacterial but is rarely applied to pathogens that cause skin infections such as Staphylococcus aureus and S. epidermidis. The purpose of this study was to determine the optimum concentration of C. calcitrans extract to inhibit the growth of S. aureus and S. epidermidis. The antibacterial activity of C. calcitrans was tested by the Kirby Bauer diffusion method. The results showed that C. calcitrans extract dissolved in DMSO with the concentrations of 5, 10, 15, and 25 mg mL-1 could produce inhibition zones on S. aureus and S. epidermidis. The average diameter of the largest inhibition zone resulted in the concentration of 25 mg mL-1, namely 10.1 ± 0.5 mm in S. aureus and 9.3 ± 0.5 mm in S. epidermidis. It can be concluded that the extract of C. calcitrans has antibacterial activity against bacteria that cause skin infections S. aureus and S. epidermidis with the optimum concentration of 25 mg mL-1.
THE EFFECT OF LIQUID TOFU WASTE AND GROWING MEDIA ON THE GROWTH AND YIELD OF LONG BEANS (Vigna sinensis L.) Fauziah, Nindi
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 9 No. 2 (2022)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2022.1758

Abstract

Long bean is a leguminous plant that has the potential to be developed into productive plants. Tofu liquid waste organic fertilizer and planting media are needed to meet nutrient needs, improve the physical and chemical properties of the soil. This study aims to understand the effect of liquid tofu waste, growing media, and their combined interaction on the growth and yield of long beans. This study used a factorial randomized block design (RBD) with 3 replications, 3 treatments of liquid tofu waste of 0 mL (T0), 150 mL (T1), and 300 mL (T2), and 3 planting media, namely cow manure compost + rice husk charcoal (M1), cow manure compost + cocopeat (M2), and cow manure compost + chopped fern (M3). If they had a significant effect, a Tukey's test at a 5% probability level would be performed. The parameters observed included the chlorophyll a and b, net assimilation rate, relative growth rate, height, number of flowers, pod length, and wet fruit weight of long beans. The results showed that the T1 and M3 treatments significantly affected chlorophyll a and b, height, number of flower, and pod length. The T1 and M2 treatments significantly affected the wet fruit weight of long beans.The T2 and M2 treatments significantly affected the net assimilation rate. Finally, the T2 and M3 treatments significantly affected the relative growth rate. There was no interaction effect found between liquid tofu waste and growing media on all research parameters.

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