African Journal of Biochemistry and Molecular Biology Research
African Journal of Biochemistry and Molecular Biology Research aims to publish high-quality, peer-reviewed research that advances biochemical and molecular understanding of living systems while supporting interdisciplinary developments across the life sciences. • Biochemical Advancement: disseminate rigorous studies in fundamental and applied biochemistry, including molecular mechanisms, metabolism, enzymes, membranes, and biomolecular interactions. • Molecular and Cellular Inquiry: encourage research in molecular biology, genetic and cellular regulation, pathogen biology, and biologically relevant analytical methods. • Life-Science Integration: promote interdisciplinary work linking biochemistry and molecular biology with biotechnology, physiology, microbiology, pharmacology, pathology, and health science. • Scientific Relevance and Application: support research that contributes to biomedical, environmental, agricultural, nutritional, and translational scientific problems. AJBMBR welcomes original research papers and related scholarly contributions in biochemistry, molecular biology, and associated life-science fields, especially studies with strong methodological grounding and clear scientific relevance. • Core Biochemistry: macromolecular biochemistry, enzymology, membrane biochemistry, nutritional biochemistry, reproductive and developmental biochemistry, and biochemical regulation of cellular processes. • Molecular and Biomedical Sciences: molecular biology, medical and clinical biochemistry, pathology, pharmacology, physiology, microbiology, parasitology, malariology, and related disease-oriented biosciences. • Biotechnology and Bioinformatics: biotechnology, computational biology, bioinformatics, molecular data analysis, and applied laboratory innovation. • Health and Natural Product Sciences: phytomedicine, food science, health science, toxicology, and biophysics relevant to biomolecular or physiological understanding. • Environmental and Applied Biosciences: environmental biochemistry and other biochemical applications connected to biological systems, sustainability, and applied life-science research.
Articles
94 Documents
<![CDATA[Effect of Administration of Copper plasma Activated water and Anesthetic on Hematological and Pancreatic tissues of Wister rats ]]>
<![CDATA[Isaac John Umaru]]>;
<![CDATA[Iseko Iyoko Kingsly]]>;
<![CDATA[Prince Kwadwo Gyasi]]>;
<![CDATA[Edom Favour Ogochukwu]]>;
<![CDATA[Tensaba Andes Akafa]]>;
<![CDATA[Jackson Soyinka Malantso]]>;
<![CDATA[Bilyaminu Habibu]]>;
<![CDATA[Kerenhappuch Isaac Umaru]]>
<![CDATA[ African Journal of Biochemistry and Molecular Biology Research Vol 2 No 1 (2025): African Journal of Biochemistry and Molecular Biology Research ]]>
Publisher : <![CDATA[Darul Yasin Al Sys ]]>
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DOI: 10.58578/ajbmbr.v2i1.4722
<![CDATA[In biomedicine, applications of PAW span from biofilm removal, wound healing, deactivation of bacteria and viruses, dentistry (for teeth disinfection and whitening), and cancer therapy. The biochemical activity of PAW is derived from synergistic effects of the highly reactive species, specifically reactive oxygen and nitrogen species (RONS). PAW is considered a biofriendly and prospective solution for biotechnology applications due to the time dependent nature of its biochemical activity because of the active species, and its economic and environmental benefits of using air rather than toxic chemicals as the raw material. Thus, the study on Effect of Administration of Copper plasma Activated water and Anaesthetic on Haematological and Pancreatic tissues of Wister rats. Material and Methods: Preparation of plasma-activated water a non-thermal micro-hollow cathode discharge (MHCD) was used to generate plasma-activated waters (CU-PAWs). Results: The results showed the haematological profile of the animals treated with 400mgkg-' had a decrease in Haemoglobin, Mean capsular Haemoglobin (11.21 ± 1.23 and 22.93 ± 21.11) compared to the controlled group (15.03 ± 1.72 and 29.93 ± 7.27) and in animals treated with 200mgkg-1 and 400mgkg-1 there is a significant decrease in the platelets (21.96 ± 23.16 and 20.89 ± 24.03) compared to animals on control group (32.08± 16.70) (table 2). Animals treated with 200mgkg-' and 400mgkg-' have a decrease in platelet (22.36 ± 4.88 and 20.89 ± 24.03) compared to animals on control (32.08 ± 16.70) and also animals treated with 400mgkg-1 have an elevated white blood cell count 173-56 ± 4.50) compared to the control group (3.66 ± 3.06). Conclusion: Cupper plasma water is safe to be used since it do not have much effect on the blood biochemistry and haematological parameters unlike the aesthetic agents cause changes on the haematological and blood biochemistry parameters. Hence, it is important to be aware of the effects of these agents before using them in experiments.]]>
<![CDATA[Cross River State Grown Andrographis paniculata Ethanol Leaf Extract Attenuates Insulin Resistance and TNF Alpha in Obese Rats ]]>
<![CDATA[B. U. Ati]]>;
<![CDATA[A. A. Asuk]]>;
<![CDATA[K. Dasofunjo]]>
<![CDATA[ African Journal of Biochemistry and Molecular Biology Research Vol 2 No 1 (2025): African Journal of Biochemistry and Molecular Biology Research ]]>
Publisher : <![CDATA[Darul Yasin Al Sys ]]>
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DOI: 10.58578/ajbmbr.v2i1.4857
<![CDATA[This study investigated the ethanol leaf extract of Andrographis paniculata and its potential role in attenuating insulin resistance and decreasing turmor necrosis factor alpha levels, raised in obese condition. Twenty five (25) rats were grouped into five (5); Group I: served as normal control and received normal saline and normal rats pellet, Group II: served as obesity control and was fed a high-fat diet but not treated, Group III: served as positive control, fed with a high-fat diet and 30 mg/kg body weight Orlistat, Group IV: fed with a high-fat diet and treated with 500 mg/kg body weight A. paniculata, and Group V: fed with a high-fat diet and treated with 1000 mg/kg body weight A. paniculata. The results showed significant (P<0.05) increases in both TNF-α and HOMAR-IR index in obese rats but decreased in obese rats treated with 30 mg/kg bw Orlistat, 500 and 1000 mg/kg bodyweight A. paniculata. It can be concluded, that A. paniculata has properties that could play a role in attenuating insulin resistance and inflammation induced by obesity.]]>
<![CDATA[Snake Venom: Its Biochemical Components and Their Uses in Medicine ]]>
<![CDATA[Isaac John Umaru]]>;
<![CDATA[Wisdom L. Benjamin]]>
<![CDATA[ African Journal of Biochemistry and Molecular Biology Research Vol 2 No 1 (2025): African Journal of Biochemistry and Molecular Biology Research ]]>
Publisher : <![CDATA[Darul Yasin Al Sys ]]>
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DOI: 10.58578/ajbmbr.v2i1.4931
<![CDATA[Snake venoms have components with diverse biological actions that are extensively studied to identify elements that may be useful in biomedical sciences. In the field of autoimmunity and rheumatology, various findings useful for the study of diseases and potential drug development have been reported. The study of disintegrins, proteins that block the action of integrins, has been useful for the development of antiplatelet agents and principles for the development of immunosuppressants and antineoplastic. Several proteins in snake venoms act on the coagulation cascade, activating factors that have allowed the development of tests for the study of coagulation, including Russell’s viper venom time, which is useful in the diagnosis of antiphospholipid syndrome. Neurotoxins with either pre- or postsynaptic effects have been used to study neurogenic synapses and neuromuscular plaques and the development of analgesics, muscle relaxants and drugs for neurodegenerative diseases. Various components act by inhibiting cells and proteins of the immune system, which will allow the development of anti-inflammatory and immunosuppressive drugs. This review summarizes the usefulness of the components of snake venoms in the fields of autoimmunity and rheumatology, which can serve as a basis for diverse translational research.]]>
<![CDATA[Determination of Mechanism of Resistance to Pyrethroid by Anopheles gambiae sensu lato from Gombe State, Nigeria ]]>
<![CDATA[Asama’u Yusuf]]>;
<![CDATA[Lazarus Joseph Goje]]>;
<![CDATA[Simon Gabriel Mafulul]]>;
<![CDATA[Nabiha Garba]]>;
<![CDATA[Nura Abubakar]]>
<![CDATA[ African Journal of Biochemistry and Molecular Biology Research Vol 2 No 1 (2025): African Journal of Biochemistry and Molecular Biology Research ]]>
Publisher : <![CDATA[Darul Yasin Al Sys ]]>
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DOI: 10.58578/ajbmbr.v2i1.5023
<![CDATA[The emergence of insecticide resistance in Anopheles gambiae sensu lato poses a significant challenge to malaria control efforts, particularly in endemic regions like Gombe, Nigeria. This study aimed to investigate the mechanisms underlying pyrethroid resistance and identify the prevalent Anopheles species in the area. Morphological identification was performed using keys from Gille and Coetzee, confirmed by molecular techniques employing SINE200 PCR for precise species characterization. The results revealed that the An. gambiae complex comprised 75% of the mosquito population, indicating its dominance in the region. Knockdown rate bioassays demonstrated a time-dependent increase in resistance to insecticides, with notable exceptions observed with deltamethrin. Susceptibility testing conducted 24 hours post-exposure confirmed that the population exhibited resistance to all tested insecticides, with DDT showing the highest resistance level. Molecular analysis identified Anopheles coluzzii as the most prevalent species in Gombe, followed by An. arabiensis. Additionally, the prevalence of kdr alleles was assessed, revealing a significant correlation between the L1014F mutation and resistance phenotypes. Specifically, the frequency of the L1014F allele was linked to increased resistance levels, while the homozygous susceptible allele was also prevalent, suggesting the potential influence of other resistance mechanisms. In conclusion, this study highlights the critical need for ongoing surveillance of insecticide resistance in Anopheles gambiae populations. It underscores the importance of understanding the genetic basis of resistance to inform effective vector control strategies. The findings emphasize that adaptive management of insecticide use, considering the dynamics of resistance and species composition, is essential for enhancing malaria control efforts in Gombe and similar regions.]]>
<![CDATA[Operational Bottlenecks and Challenges of Slaughterhouse Workers in Tamale Metropolis Ghana ]]>
<![CDATA[Solomon Ossom Asare]]>;
<![CDATA[Prince Kwadwo Gyasi]]>;
<![CDATA[Okrah George Hassan]]>;
<![CDATA[Ogwa Oluwafunmike Abiodun]]>
<![CDATA[ African Journal of Biochemistry and Molecular Biology Research Vol 2 No 1 (2025): African Journal of Biochemistry and Molecular Biology Research ]]>
Publisher : <![CDATA[Darul Yasin Al Sys ]]>
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DOI: 10.58578/ajbmbr.v2i1.5024
<![CDATA[This study focused on assessing operational bottlenecks and challenges of slaughterhouse workers in Tamale metropolis Ghana. The research aims to provide insights into the distribution and intensity of various activities near within the abattoir, this including. Access to potable water for the washing of carcasses after processing is a challenge, Difficulty with disposal of solid waste which is far away from the abattoir, Availability drainage for liquid waste management in the slaughterhouse, the slaughterhouse is not shielded sufficiently to prevent the entry of pests, and Non-availability of fumigation activities as a pest control measure as well as Non-availability of separate areas for skinning /dehairing process of the carcass. The study aims to evaluate the operational bottlenecks and challenges of slaughterhouse workers in Tamale metropolis Ghana. The research employed several spatial analysis techniques, such as density mapping, and hotspot analysis, to assess the spatial patterns and relationships between the abattoir and its surrounding areas. The spatial analysis identified potential issues such as the presence of critical infrastructure in close proximity to the slaughterhouse. Thus, solution to those challenges.]]>
<![CDATA[The Role of Telomeres and Telomerase in Cellular Aging and Disease: Mechanisms, Implications, and Therapeutic Interventions ]]>
<![CDATA[Grace Christopher]]>;
<![CDATA[Lazarus Joseph Goje]]>
<![CDATA[ African Journal of Biochemistry and Molecular Biology Research Vol 2 No 2 (2025): African Journal of Biochemistry and Molecular Biology Research ]]>
Publisher : <![CDATA[Darul Yasin Al Sys ]]>
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DOI: 10.58578/ajbmbr.v2i2.5151
<![CDATA[Telomeres, the protective caps at the ends of chromosomes, play a crucial role in maintaining genomic stability. This review explores the structure and function of telomeres, the mechanisms of telomere shortening, and the role of telomerase in counteracting this process. Telomere shortening is a natural consequence of cell division and is associated with cellular aging, senescence, and apoptosis. The review delves into the molecular biology of telomeres, highlighting the significance of the shelterin complex in protecting telomeres from degradation and fusion. It also discusses the genetic and epigenetic factors influencing telomere length, the impact of oxidative stress and inflammation on telomere attrition, and potential interventions to preserve telomere length. The review concludes with recommendations for lifestyle changes and therapeutic strategies to mitigate telomere shortening and promote healthy aging.]]>
<![CDATA[Effect of Aqueous Extract of Achyranthes aspera Leaves on Antiretroviral Drug-Induced Hepatotoxicity in Rats ]]>
<![CDATA[Isaac John Umaru]]>;
<![CDATA[Mary Athanasius Udoh]]>;
<![CDATA[Tensaba Andes Akafa]]>;
<![CDATA[Joseph Oteng]]>;
<![CDATA[Ingwu Joseph Akem]]>;
<![CDATA[Ocheifa Mathew Ngbede]]>;
<![CDATA[Ashaka Fidelis Utioukpan]]>;
<![CDATA[Aboki Nwunuji Mijinyawa]]>;
<![CDATA[Ogholo Ogholo Ekup]]>
<![CDATA[ African Journal of Biochemistry and Molecular Biology Research Vol 2 No 2 (2025): African Journal of Biochemistry and Molecular Biology Research ]]>
Publisher : <![CDATA[Darul Yasin Al Sys ]]>
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DOI: 10.58578/ajbmbr.v2i2.5183
<![CDATA[Achyranthes aspera leaves are believed to reverse drug resistance and increase the efficacy of current drugs. Achyranthes aspera leaves contain many secondary metabolites needed for the redressal of diseases and ailment. Thus, the ART-induced hepatotoxicity in rats. Methodology: Thirty (30) albino rats were divided into 5 groups of 6 each and treated as follows: Group A (no antiretroviral drugs, no extract); group B (antiretroviral drugs alone); group C (extract alone); group D (antiretroviral drug plus 40 mg/kg extract); group E (antiretroviral drug plus 80 mg/kg extract). All treatment lasted for twenty-eight days. Blood samples were collescted and serum ALT and AST determined using UV-spectrophotometer. The mean (± S.E.M) of data were calculated and further analysed for statistical significance using graph Pad Prism 5.0. Results: Mean serum ALT were 36.8 ± 20.24, 56.11 ± 6.12, 54.6 ± 24.18, 90.40 ± 11.14, 88.36 ± 13.10 and that of AST were 143.8 ± 20.24, 208.4 ± 16.13, 60.0 ± 24.18, 164.40 ± 11.14, 52.36 ± 16.14 for groups A, B, C, D, and E respectively. There was a statistically significant difference between the mean values of serum AST for group B and those for group C. However, there was no statistically significant difference between the ALT values for the test and control groups of rats (p value >0.999) Also, there was no statistically significant difference between the mean values of AST for group B and those of groups A, D, E. Conclusions: This extract at a higher concentration should add to the treatment of HIV in synergy with antiviral drugs, However, the extract shown no significant reduction of serum ALT and AST in ART treated rats.]]>
<![CDATA[Exploring the Dynamics of Enzyme Activity: Environmental and Biological Influences ]]>
<![CDATA[Muhammad Akram]]>;
<![CDATA[Urooj Rehman]]>;
<![CDATA[Misbah Ahmed]]>;
<![CDATA[Isaac John Umaru]]>
<![CDATA[ African Journal of Biochemistry and Molecular Biology Research Vol 2 No 2 (2025): African Journal of Biochemistry and Molecular Biology Research ]]>
Publisher : <![CDATA[Darul Yasin Al Sys ]]>
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DOI: 10.58578/ajbmbr.v2i2.5268
<![CDATA[The work presents the classification of enzymes and factors that are affecting enzymatic reactions in the living systems. Factors such as temperature, pH, enzyme concentration, substrate concentration, factors of inhibition, factors of activation, and incubation time are important in influencing enzyme reactions, which are responsible for controlling components in living systems. The majority of enzymes are three-dimensional molecules with complex molecular networks and sensitive to various environmental factors.]]>
<![CDATA[Phytochemistry and Pharmacology of Oxystelma Esculentum ]]>
<![CDATA[Abdul Wadood Chishti]]>;
<![CDATA[Muhammad Akram]]>;
<![CDATA[Urooj Rehman]]>;
<![CDATA[Fahad Said Khan]]>;
<![CDATA[Momina Iftikhar]]>;
<![CDATA[Isaac John Umaru]]>
<![CDATA[ African Journal of Biochemistry and Molecular Biology Research Vol 2 No 2 (2025): African Journal of Biochemistry and Molecular Biology Research ]]>
Publisher : <![CDATA[Darul Yasin Al Sys ]]>
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DOI: 10.58578/ajbmbr.v2i2.5269
<![CDATA[Several phytochemicals, including glycosides, alkaloids, flavonoids, and saponins, are present in the widespread medicinal herb Oxystelmaesculentum. These physiologically active substances contribute to the herb's pharmacological properties, which include anti-inflammatory, antioxidant, antibacterial, and antidiabetic effects. Research studies have shown that O. esculentum can be used medicinally to treat a variety of ailments, such as fever, pain, respiratory issues, and digestive issues. It may also have neuroprotective properties and help in blood sugar regulation. Even though its therapeutic qualities appear promising, more research must be done to verify its efficacy, safety, and potential for drug development.]]>
<![CDATA[Cutting-Edge Discoveries in Cancer Biology and Therapeutic Development ]]>
<![CDATA[Muhammad Akram]]>;
<![CDATA[Muhammad Abdullah]]>;
<![CDATA[Momina Iftikhar]]>;
<![CDATA[Isaac John Umaru]]>
<![CDATA[ African Journal of Biochemistry and Molecular Biology Research Vol 2 No 2 (2025): African Journal of Biochemistry and Molecular Biology Research ]]>
Publisher : <![CDATA[Darul Yasin Al Sys ]]>
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DOI: 10.58578/ajbmbr.v2i2.5306
<![CDATA[Growth in precision medicine, immunotherapy, genetics, and new therapeutic approaches is driving the rapid progression of cancer science and drug discovery. Greater tailor-made treatments attacking specific genetic abnormalities and immune systems have become an alternative to the one-size-fits-all approach, bringing renewed hope to patients for improved outcomes and fewer adverse effects. The most critical growth impacting the face of cancer research and drug discovery is summarized in this brief. Next-generation sequencing (NGS) and other methods enable complete genomic profiling, which identifies actionable mutations and guides targeted therapy. Stepping away from blanket chemotherapy, scientists can develop more effective and less toxic drugs by understanding the specific mutations that create each patient's cancer. By blocking the brakes of the immune system, these drugs allow T cells to better fight cancer cells. However, research is ongoing to maximize these treatments' efficacy and safety, particularly in the case of solid tumors. Targeting some of the TME components, such as the extracellular matrix or TME-dwelling immune cells, could provide new treatment strategies. Huge amounts of genetic information are being evaluated, potential medicine candidates are being identified, and patient outcomes are being forecast by AI algorithms. In addition, machine learning algorithms are being employed to improve patient stratification, optimize clinical trial design, and personalize treatment regimens using real-time data. Combination Treatments: Combination therapies employing multiple mechanisms of action are gaining increasing popularity as a result of the flexibility and complexity of cancer. For maximizing efficacy and overcoming resistance, researchers are incorporating immunotherapy with targeted therapy, chemotherapy, radiation therapy, and other modalities. The goal is to keep tumor cells from evading treatment by attacking cancer from a variety of angles. These combinations are currently under investigation in clinical trials, which have had promising results, particularly in cancers such as breast, lung, and melanoma.]]>
