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Antidiabetic Potential of Khaya Anthotheca Methanolic Leaves Extract in Streptozotocin Diabetes Induced Rats Umaru, Kerenhappuch Isaac; Tansaba, Akafa Andes; Umaru, Isaac John; Ugoeze, Egeonu Stephen; Nanmar, Chakfa; David, Bando Christopher
Journal of Multidisciplinary Science: MIKAILALSYS Vol 2 No 2 (2024): Journal of Multidisciplinary Science: MIKAILALSYS
Publisher : Darul Yasin Al Sys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/mikailalsys.v2i2.3191

This research evaluated antidibetic potential of methanolic leaves extracts of Khaya anthotheca in streptozotocin induced albino rats. Khaya anthotheca a member of Meliaceae family is traditionally used for treating several ailments. Diabetes is a chronic condition that develops when the pancreas is unable to secrete sufficient amounts of insulin or when the body fails to utilise the insulin that is produced. The leaves were harvested in Sukundi road Wukari and air dried for four weeks, the leaves were pulverized into powder using manual blender and stored in an air tight container. Extraction was done with absolute methanol; the extract was filtered using mesh and the filtrate was concentrated at 68oC. two grams of the filtrate were used for the study; the remainder was used to treat the animals. Using 40 mg/kg body weight of streptozotocin, the animals were divided into 5 groups of 4 each. Following a fourteen-day course of treatment, the animals were sacrificed; the blood was drawn through heart puncture and tested for blood sugar using a glucometer. One-way analysis of variance (ANOVA) was used for statistical analysis. Result of blood sugar level showed significant reduction in all the test groups (p<0.05) except group 2 which showed no significant reduction when compared to the normal control. Conclusion: This study therefore suggests the use of Khaya anthotheca as a diabetic agent and for treatment of diabetes. Further studies are needed to discover the bioactive constituent of the plant responsible for this anti-diabetic activity as well as other pharmacological activities in clinical trials.

GC-MS Profiling and Pathogenic Effect of Khaya Anthotheca Methanolic Leaves Extract on Some Selected Fungi Umaru, Isaac John; Shadrach, Philip; Adi, Godwin Baaku; Ugoeze, Egeonu Stephen; Nanmar, Chakfa; Akafa, Andes Tensaba; Umaru, Kerenhappuch Isaac; David, Bando Christopher
Journal of Multidisciplinary Science: MIKAILALSYS Vol 2 No 2 (2024): Journal of Multidisciplinary Science: MIKAILALSYS
Publisher : Darul Yasin Al Sys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/mikailalsys.v2i2.3192

This research evaluated GC-MS profile, and pathogenic effect of methanolic leaves extracts of Khaya anthotheca on Some Selected fungi. Khaya anthotheca a member of Meliaceae family is traditionally used for treating several ailments. The leaves were harvested in Sukundi road Wukari and air dried for four weeks, the leaves were pulverized into powder using manual blender and stored in an air tight container. Extraction was done with absolute methanol; the extract was filtered using mesh and the filtrate was concentrated at 68oC. Only two grams of the filtrate were used for the GC-MS study. One-way analysis of variance (ANOVA) was used for statistical analysis. The GC-MS result revealed 50 constituents. Result of Methanolic extracts of leaves of Khaya anthotheca only showed significant activity against Aspergillus niger at dose100 µg/mL, Candida tropicalis and Fusarium oxysporium at dose 500 µg/mL. This study supports the use of Khaya anthotheca by indigenous herbalists by providing scientific proof of the plant's ability to treat pothegenic diseases. To establish the effectiveness of this plant in the management of fungal effections, more clinical trials at the clinical levels are needed.

Assessment of Hyperglycemic Potentials of Khaya Anthotheca Stem-Bark Extracts on Alloxan-Induced Diabetic Rats Umaru, Kerenhappuch Isaac; Nanmar, Chakfa; Umaru, Isaac John; Ugoeze, Egeonu Stephen; Tansaba, Akafa Andes; David, Bando Christopher
Asian Journal of Science, Technology, Engineering, and Art Vol 2 No 3 (2024): Asian Journal of Science, Technology, Engineering, and Art
Publisher : Darul Yasin Al Sys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/ajstea.v2i3.3183

Introduction: Medicinal plants have been the basis of treatment of various diseases in African traditional medicine as well as other forms of treatment from diverse cultures of the world. Khaya anthotheca is a member of the family Meliaceae, a native to Africa. It is a medicinal plant widely used for several therapeutic purposes. Herein, phytochemical study, hyperglycaemic investigations was carried out on the methanol extract of Khaya anthotheca Stem-bark. Materials and Methods: However, 35 rats were distributed into seven groups to evaluate the anti-diabetic activity of K. anthotheca stem-bark methanol extract at the dosage range of 50-400 mg/kg/b.wt. The analysis was done using the software-SPSS one-way ANOVA at a significance level p<0.05. Results: The result of the anti-diabetic study reveals that extract elicited substantial (p<0.05) inhibition of hyperglycaemic. The plant Khaya anthotheca stem-bark methanol extract displayed profound (p<0.05) anti-hyperglycaemic action at the dosage of 200 mg/kg/b.wt, 300 mg/kg/b.wt. and 500 mg/kg/b.wt. against induced diabetic rats. Conclusion: This study therefore suggests the use of Khaya anthotheca as a hypoglycaemic agent and for treatment of diabetic. Further studies are needed to discover the bioactive constituent of the plant responsible for this anti-diabetic activity.

Phytochemical Profiling, and Antifungal Potentials of Stem-Bark Extracts of East African (Khaya Anthotheca) Umaru, Kerenhappuch Isaac; Ugoeze, Egeonu Stephen; Umaru, Isaac John; Nanmar, Chakfa; Tansaba, Akafa Andes; David, Bando Christopher
Asian Journal of Science, Technology, Engineering, and Art Vol 2 No 3 (2024): Asian Journal of Science, Technology, Engineering, and Art
Publisher : Darul Yasin Al Sys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/ajstea.v2i3.3185

Fungal infection is an infectious disease that is commonly implicated as aetiologic agents that contribute to the increasing burden of morbidity and mortality in developing countries as a result misdiagnosis or improper diagnosis. This study determined the profiling and antifungal potentials of Stem-Bark Extracts of East African (Khaya Anthotheca). The methanol solvent was used for disc diffusion assay. The inhibitory concentration of the extract was performed by broth dilution method and zone of inhibition was studied by disc diffusion method at the concentration of 50, 100, 250, and 500 in DMSO. Nystatin was used as the reference control for antifungal study. The extract showed maximum inhibition potential sof zone of inhibition against most of the pathogen (Aspergillus niger, Aspergillus flavus, Candida tropicalis and Fusarium oxysporium) used at concentration 50ppm to 500ppm. The zone of inhibition for 500ppm is shown as (319.12± 0.11, 19.23 ± 0.12, 20.33±0.23* and 18.34±0.21*mm respectively). The extract showed minimum inhibition potential against Aspergillus flavus in all the concentration when compared with the control as well as to the other pathogens. Candida tropicalis and Fusarium oxysporium was found to be more sensitive to the methanol extract followed by Aspergillus flavus and Aspergillus niger lastly. The extract was found to be rich in phytochemical, with about 20 different chemical constitutes, with 12.14- Pentane, 3-ethyl-2,4-dimethyl-, 14.66-3-Hexen-2-one and 27.23-2-Nonenal, 2-pentyl-as the highest. The Present study indicates the potential usefulness of methanol extract of extract of khaya anthotheca as antifungal agent. Thus, has therefore, contributed to the pool of knowledge already available in this area of research. Considering the effects that both phytochemicals and its potentials as antifungal, it would be expedient to conduct further studies to assess its cancer potentials.

Evaluation of Potential in Wonderful Kola (Buchiolizia coreica) Seed Extract on Streptozotocin Induced Type 2 Diabetes in Male Wister Rats Umaru, Isaac John; Onyinyechi, Nwachukwu Favour; Tansaba, Akafa Andes; Nanmar, Chakfa; Umaru, Kerenhappuch Isaac; Ugoeze, Egeonu Stephen; Galam, Nanyak Zingfa
African Multidisciplinary Journal of Sciences and Artificial Intelligence Vol 1 No 1 (2024): African Multidisciplinary Journal of Sciences and Artificial Intelligence
Publisher : Darul Yasin Al Sys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/amjsai.v1i1.3388

Diabetes mellitus is referred to as a metabolic disorder in which there is high glucose level in the blood as a result of insulin deficiency, resistance or both. Globally, it has been deducted that the adult population with diabetes will rise by 69% for the year 2030. Thus, Bucholzia Coriacea (B. Coriacea) a perennial plant belonging to the family capparidaceae and genus Bucholzia is popularly known as wonderful kola. It’s also known as wonderful kola, its other name is called Garcinia kola .it has a long history of use in west Africa and central Africa, thus, the study on evaluation of potential in wonderful kola (buchiolizia coreica) seed extract on streptozotocin induced type 2 diabetes in male wister rats. Material and Methods: Fresh seeds of B. coriacea were plucked and washed with distilled water, the filtrate was concentrated using rotary evaporator and water bath. The extract was then weighed and stored in a refrigerator. 100g of crude extract and 1000mg of metformin was properly dissolved in 10ml of distilled water. The rats were carefully selected and separated into cages. 7 albino rats were used for normal control while 4 rats were kept in each of the remaining cages, and 6 replications were obtained, the test was carried out carefully by injecting the alloxan into the rats intraperitoneally and infecting them with diabetes mellitus, the following sets were made: normal group, negative and positive control group, while three groups were for extracts dosage. With exception of the normal control, all the groups were induced with diabetes. The extract (stem bark of Mangifera indica) will be administered orally to the three set of groups separately (i.e., one extract to one set). Determination of Fasting Blood Glucose Level by Intestinal Glucose Absorption. Results: Treatment groups receiving methanol Wonder Kola seed extract showed varying degrees of improvement in these parameters compared to the negative control group. Notably, Group VI, receiving the highest dose of the extract, demonstrated the most significant reduction in urea levels, suggesting a potential protective effect on renal function. The study investigated the effects of Wonder Kola seed extract on serum electrolytes, urea, and creatinine levels in alloxan-induced diabetic albino rats. Notably, significant variations were observed in these biochemical parameters across the different treatment groups. The study assessed the effects of methanol Wonder Kola seed extract on liver enzymes (AST, ALT, ALP) and serum protein levels (total protein, albumin, total bilirubin, direct bilirubin). Significant variations were observed in these parameters among the treatment groups compared to the normal and negative control groups. Treatment with the extract led to notable reductions in liver enzyme levels, indicating potential hepatoprotective effects. Conclusion: The findings of this study demonstrate the potential pharmacological benefits of methanol Wonder Kola seed extract in managing diabetes mellitus and associated complications.

Evaluation of Potential in Wonderful Kola (Buchiolizia coreica) Seed Extract on Streptozotocin Induced Type 2 Diabetes in Male Wister Rats Umaru, Isaac John; Onyinyechi, Nwachukwu Favour; Tansaba, Akafa Andes; Nanmar, Chakfa; Umaru, Kerenhappuch Isaac; Ugoeze, Egeonu Stephen; Galam, Nanyak Zingfa
African Multidisciplinary Journal of Sciences and Artificial Intelligence Vol 1 No 1 (2024): African Multidisciplinary Journal of Sciences and Artificial Intelligence
Publisher : Darul Yasin Al Sys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/amjsai.v1i1.3388

Diabetes mellitus is referred to as a metabolic disorder in which there is high glucose level in the blood as a result of insulin deficiency, resistance or both. Globally, it has been deducted that the adult population with diabetes will rise by 69% for the year 2030. Thus, Bucholzia Coriacea (B. Coriacea) a perennial plant belonging to the family capparidaceae and genus Bucholzia is popularly known as wonderful kola. It’s also known as wonderful kola, its other name is called Garcinia kola .it has a long history of use in west Africa and central Africa, thus, the study on evaluation of potential in wonderful kola (buchiolizia coreica) seed extract on streptozotocin induced type 2 diabetes in male wister rats. Material and Methods: Fresh seeds of B. coriacea were plucked and washed with distilled water, the filtrate was concentrated using rotary evaporator and water bath. The extract was then weighed and stored in a refrigerator. 100g of crude extract and 1000mg of metformin was properly dissolved in 10ml of distilled water. The rats were carefully selected and separated into cages. 7 albino rats were used for normal control while 4 rats were kept in each of the remaining cages, and 6 replications were obtained, the test was carried out carefully by injecting the alloxan into the rats intraperitoneally and infecting them with diabetes mellitus, the following sets were made: normal group, negative and positive control group, while three groups were for extracts dosage. With exception of the normal control, all the groups were induced with diabetes. The extract (stem bark of Mangifera indica) will be administered orally to the three set of groups separately (i.e., one extract to one set). Determination of Fasting Blood Glucose Level by Intestinal Glucose Absorption. Results: Treatment groups receiving methanol Wonder Kola seed extract showed varying degrees of improvement in these parameters compared to the negative control group. Notably, Group VI, receiving the highest dose of the extract, demonstrated the most significant reduction in urea levels, suggesting a potential protective effect on renal function. The study investigated the effects of Wonder Kola seed extract on serum electrolytes, urea, and creatinine levels in alloxan-induced diabetic albino rats. Notably, significant variations were observed in these biochemical parameters across the different treatment groups. The study assessed the effects of methanol Wonder Kola seed extract on liver enzymes (AST, ALT, ALP) and serum protein levels (total protein, albumin, total bilirubin, direct bilirubin). Significant variations were observed in these parameters among the treatment groups compared to the normal and negative control groups. Treatment with the extract led to notable reductions in liver enzyme levels, indicating potential hepatoprotective effects. Conclusion: The findings of this study demonstrate the potential pharmacological benefits of methanol Wonder Kola seed extract in managing diabetes mellitus and associated complications.

Evaluation of Antibacterial Activity and Toxicity Effects of Newbouldia Stem Bark Extract Umaru, Kerenhappuch Isaac; Umaru, Isaac John; Tansaba, Akafa Andes; Nanmar, Chakfa; Ugoeze, Egeonu Stephen
African Journal of Biochemistry and Molecular Biology Research Vol 1 No 1 (2024): African Journal of Biochemistry and Molecular Biology Research
Publisher : Darul Yasin Al Sys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/ajbmbr.v1i1.3350

Newbouldia leavis is a boundary tree. it is a medium size angiosperm which belongs to the Bignoniaceae family. It is a fast-growing evergreen shrub or small tree. This study was carried out to investigate antibacterial activity of the methanol extract of Newbouldia stem bark. The antibacterial activity of the methanol extracts was determined by the agar well diffusion method. Three standard bacterial strains: Escherichia coli, Staphylococcus aureus, and Klebsiella pneumoniae were used for the antibacterial assay. Result of screening plant extracts for antibacterial activity showed that most of the organisms were sensitive to the extract. The antibacterial test utilizing well diffusion assay showed that Gram-positive bacteria were more susceptible to the plant extract compared to Gram-negative bacteria. This study supports the use of Newboulda leavis in traditional medicine as well as highlights the need to further explore the potentials of the plant extract as antibacterial agent.

Hypoglycemic Potentials of Newbouldia laevis Stem Bark Extract in Alloxan-Induced Diabetic Rats Umaru, Isaac John; Chisom, Gabriel Favour; Umaru, Kerenhappuch Isaac; Tansaba, Akafa Andes; Nanmar, Chakfa; Ugoeze, Egeonu Stephen
African Journal of Biochemistry and Molecular Biology Research Vol 1 No 1 (2024): African Journal of Biochemistry and Molecular Biology Research
Publisher : Darul Yasin Al Sys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/ajbmbr.v1i1.3351

This study was carried out to investigate the antidiabetic of the methanol extract of Newbouldia stem bark and to determine its safety and toxicity. Alloxan (150 mg/kg) was administered to albino rats via the oral route. The diabetic rats were then placed in 6 groups, following stabilization of hyperglycemia. The first group was non-diabetic, the second and third group were the diabetic control. The next three groups received, each day, 100, 200 and 400 mg/kg body weight of the methanol extract Newbouldia laevis and the second group received a reference standard, metformin (200mg/kg). Treatment was via the oral route for 14 days and fasting blood sugar level was monitored over this period. Acute toxicity (oral and intraperitoneal) studies on the extract was carried out. Blood glucose levels from day 1 to 14 days of treatment increased significantly (p <0.05) in all the treatment groups, except group 3 (positive control) which showed no significant difference compared to the normal control group. This study supports the use of Newbouldia laevis in traditional medicine as well as highlights the need to further explore the potentials of the plant extract as an antidiabetic.

Antibacterial and Antioxidant Capacity of Methanol Root Extract of Mangifera Indica Umaru, Isaac John; Ugbedeojo, Sule John; Tansaba, Akafa Andes; Nanmar, Chakfa; Umaru, Kerenhappuch Isaac; Ugoeze, Egeonu Stephen
African Journal of Biochemistry and Molecular Biology Research Vol 1 No 1 (2024): African Journal of Biochemistry and Molecular Biology Research
Publisher : Darul Yasin Al Sys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/ajbmbr.v1i1.3352

Recent years have witnessed enhanced research work reported on plants and plant products. In this regard, plants with traditional therapeutic usage are being screened more efficiently to be considered as a substitution or as a better alternative agent for Human Pathogens. Antioxidant activity, antibacterial, antifungal properties, colour, phytochemical and FT-IR spectral analysis of flowers belonging to Hibiscus moscheutos was determined. Radical scavenging activity of sample extracts were determined based on the percent inhibition of DPPH and ferric reducing antioxidant power (FRAP) assays. Total phenolics were estimated based on the Folin–Ciocalteu method, while, vanillin–HCl and aluminum chloride methods were employed to estimate total tannins and flavonoids in the sample extracts, respectively. To determine total flavonols and anthocyanin contents, spectrophotometric method was employed. For antibacterial activities, modified agar disk diffusion method was adopted. Results: The crude extracts of hexane, dichloromethane, chloroform, ethyl acetate and methanol, extracts with concentration levels at 100, 200, 300 and 400 mg/mL were shown to significantly affect the inhibition of bacterial selected. The presence of phytochemicals compounds possesses the high antibacterial activities of this crude extracts. This study proved that Mangifera Indica crude extracts could be useful for inhibiting the selected pathogens which has an antioxidant potential as well as a natural bacterial agent.

Hepatocurative Effect of Methanol Extract of Newbouldia laevis Leaves in Alloxan-Induced Liver Damage in Albino Rats Umaru, Isaac John; Rebecca, Tsokwa Punarimam; Tansaba, Akafa Andes; Nanmar, Chakfa; Umaru, Kerenhappuch Isaac; Ugoeze, Egeonu Stephen; Danjuma, Tyem Lawal
African Journal of Biochemistry and Molecular Biology Research Vol 1 No 1 (2024): African Journal of Biochemistry and Molecular Biology Research
Publisher : Darul Yasin Al Sys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/ajbmbr.v1i1.3354

The aim of the study is to evaluate the effect (Hepatocurative and antibacterial potential) of Newboulda laevis leaves in alloxan-induced liver damage in Wister Albino Rats. The leaves of Newbouldia laevis was bought from a local market in Wukari, Taraba State. The leaves of Newbouldia laevis was chop and then pulverized into fine powder. The methanol crude extracts andthirty-two albino Wistar rats weighing 90-220 g was used for this research. Liver toxicity was induced in male rats by an intraperitoneal single dose of 150 mg/kg body weight. samples were subjected to biochemical estimation of liver function. The result showed a significant (p≤0.05) increase in serum AST level for group II (60.6 ± 1.12) which was administered high dose of Alloxan and serves as the negative control group, when compared to the normal control group I (35.09 ± 2.07) which only received feed and water. From the result described in table 4.0 above, it showed a significant (p≤0.05) increase in ALT level for group II (58.11 ± 0.42) negative control when compared with group I (20.00 ± 0.41) normal control. Group III (29.98 ± 0.12) which received standard drug showed a significant decrease in ALT which is a sign that there is some level of repair on the liver cells. Group IV (36.22 ± 0.s70), group V (30.00 ± 0.27) and group VI (29.99 ± 0.12) which received 100, 200 and 400 mg/kg body weight of Newbouldia laevis leaves extract respectively when compared with the negative control group II, showed a significant decrease in ALT level. There was a significant (p≤0.05) increase in ALP level for group II (255.80 ± 1.12) which received Alloxan when compared with the normal control group I (80.21 ± 0.44) providing evidence to support the increase in ALT and AST level in group II, suggestive of liver injury. The treatment groups III, VI, V and VI showed significant (p<0.05) difference, when compared to the negative control group. No significant (p<0.05) difference between group V and VII. It was deduced that the total protein for group II (10.95 ± 0.37) significantly (p<0.05) decreased when compared to the normal control group I (14.13 ± 0.55). This is because low levels of total protein in the blood can occur because of impaired function of the liver. Administration of Newbouldia laevis leaves extract significantly (p<0.05) elevated total protein levels for group IV (10.99 ± 0.41), V (12.76 ± 0.41) and VI (12.98 ± 0.58). Albumin being the most abundant protein in the blood, equally followed same pattern as total protein, with Albumin level of (10.83 ± 0.10), (6.13 ± 0.05), (9.82 ± 0.18) (6.75 ± 0.13), (7.99 ± 0.10) and (9.60 ± 0.13) for group I, II, III, IV, V and V respectively. Thus, emphasizing the potential of the extract as a source agent for medication for ailment and diseases.

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