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Jurnal Akademika Biologi
Published by Universitas Diponegoro
ISSN : -     EISSN : 26219824     DOI : -
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology
Jurnal Akademika Biologi diterbitkan oleh Departemen Biologi Fakultas Sains dan Matematika Universitas Diponegoro Semarang. Jurnal ini sebagai media publikasi hasil karya ilmiah lulusan S1 Departemen Biologi. Jurnal Akademika Biologi menerima artikel-artikel yang berhubungan dengan bidang ilmu biologi.
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 11 Documents
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Search results for , issue "Vol. 6 No. 2 April 2017" : 11 Documents clear
PENGARUH LIMBAH CAIR TAHU TERHADAP PERTUMBUHAN POPULASI CHLORELLA PYRENOIDOSA H. CHICK DALAM SKALA LABORATORIUM Fatmawati Munir; Riche Hariyati; Erry Wiryani
Jurnal Akademika Biologi Vol. 6 No. 2 April 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Chlorella pyrenoidosa H.Chick is one of abundant microalgae in Indonesia and mostly cultivated due to its characters which are fast and easily transplanted. Tofu liquid waste contains nutrient which is needed by mikroalga because it contains inorganic content such as carbon, nitrogen, phosphorus, potassium. This research is to study the influence of difference concentration of tofu liquid waste towards population growth of C. pyrenoidosa and concentration level of  tofu liquid waste towards population growth of C. pyrenoidosa. The method is by cultivating C. pyrenoidosa H.Chick through the water media which has been given any tofu liquid waste concentration which functions as nutrient source for microalgae. The tofu liquid waste concentration  is consisting of0% as control, 15%, 30%, 45% and 60% and it’s conducted by three times repetition.  The obtained data was tested by ANOVA. As the F score was different then continued by Duncan test. The result shows that the optimal peak of microalgae’s population growth could befound in media with 15% concentration of tofu liquid waste, 337.500 cell/ml, then another control(0%), 170.000 cell/ml and 30%, 119.167 cell/ml, 45%, 81.667 cell/ml. The solidity of lowest population in  60%  concentration showed 65.833 cell/ml. This  findings  has showed that thedifferent handling of concentration towards tofu liquid waste has influenced on C. pyrenoidosapopulation growth. The optimal growth reaches at 15% concentration of  tofu liquid waste. Keywords: Chlorella pyrenoidosa, population, waste, liquid, tofu
EFEKTIVITAS KOMBINASI EKSTRAK BAHAN HERBAL (Mengkudu, Pepaya, Kunyit) TERHADAP DAYA HAMBAT PERTUMBUHAN Aeromonas hydrophila SECARA IN VITRO Dadi Pratama; Agung Budiharjo
Jurnal Akademika Biologi Vol. 6 No. 2 April 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

. One of the alternative to overcome bacterial diseases of fish is by using herbal material that contain antibacterial. This study aimed to test the effectiveness of a combination of herbal material extract (noni, papaya and turmeric) in controlling the growth of bacteria Aeromonas hydrophila in-vitro. The research design used was Completely Randomized Design (CRD), which consists of 33 treatments and 3 replications. The parameters measured were the absorbance value (λ = 600 nm) of A. hydrophila growth after 18 hours of incubation and was measured by multiskan GO microplate spectrophotometer. This research was conducted through a two-stage test, the test MIC and FIC using microdilution method. MIC test result showed the minimum dosage of each herbal extract that is 1 µl/ml for noni extract, 2 µl/ml for papaya extract, and 1 µl/ml for turmeric extract. Values obtained FIC index is 0.250 (combination MP), and 0.375 (a combination of MK and PK). The combination of herbal material each extract showed synergistic interaction with FIC index values ≤ 0.5. Formula of the most effective and efficient than any combination is 1/8x noni and 1/4x papaya, 1/4x noni and 1/8x turmeric, 1/4x papaya and 1/8x turmeric.  Keywords: Antibacterial, A.hydrophila, FIC Indeks
STRUKTUR KOMUNITAS PLANKTON DI KAWASAN WANA WISATA CURUG SEMIRANG KECAMATAN UNGARAN BARAT, SEMARANG Delliana Ratna Sari; Jafron Wasiq Hidayat; Riche Hariyati
Jurnal Akademika Biologi Vol. 6 No. 2 April 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Objective of the study was to know the structure of plankton community and to know the influential physical and chemical factors in the structure of plankton community in Semirang. This research was conducted on 6 stations with different water body condition. Results showed that the number of plankton species obtained were 12 species in the Bacillariophyta Division. Plankton species that widely found were Achnanthes sp, Cymbella tropica and Cocconeis pediculus. The relative abundance index categorized into the dominant species category. The plankton diversity index were mostly included in the moderate category. Similarity index belongs to the category of even distribution, none dominance of a kind. The similarity index is mostly included in the high category. Physical and chemical factors in these waters have good water quality for plankton life.  Keywords: plankton community structure, freshwater, ecotourism waterfall Semirang 
MICROBIAL FUEL CELL (MFC) MENGGUNAKAN BAKTERI BACILLUS SUBTILIS DENGAN SUBSTRAT LIMBAH SEPTIC TANK SERTA PENGARUHNYA TERHADAP KUALITAS LIMBAH Fitra Adi Prayogo; Agung Suprihadi; Budi Raharjo
Jurnal Akademika Biologi Vol. 6 No. 2 April 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

The power plant was still dependent on non-renewable energy is one factor in Indonesia fixed with the problems of electric energy crisis. Several lecturer have shown that B. subtilis can produced electricity through Microbial Fuel Cell (MFC). The purpose of this study is to measured the values of voltage, electric current, and power density as well as changes in the quality of waste water septic tank. Waste in a vacuum toilet can be of service "Doremon Jaya", sterilized and counted the value of the quality of wastes. B. subtilis inoculum was then inoculated into a bioreactor containing sterile waste septic tank with 4 different concentrations of 0%, 1%, 2% and 3% (v/v). Inoculum was incubated for 12 hours prior to observing. The result showed that B. subtilis with inoculum concentration of 3% (v/v) produced the greatest voltage, which is 299.70 mV on 4-hour with strong current and power density 0.2997 mA 8.98 mW / cm2. COD can reduced by up to 31.18% at the inoculum concentrations of 3% (v/v), while the value of BOD reduced by up to 25.03% at the inoculum concentrations of 3% (v/v). DO value also experienced the percentage decreased to 34.25% or decreased to 0.96 at the inoculum concentrations of 2% (v/v). pH value in the treatment of inoculum concentrations of 0%, 1%, 2% and 3% (v / v) decreased after the fermentation was consecutively 5.4, 4.8, 4.9, and 4.2  Kata kunci : Bacillus subtilis, Microbial Fuel Cell, Septic  tank, Wastewater quality
UJI KUALITAS MADU PADA BEBERAPA WILAYAH BUDIDAYA LEBAH MADU DI KABUPATEN PATI Iffa Illiyya Fatma; Sri Haryanti; Sri Widodo Agung Suedy
Jurnal Akademika Biologi Vol. 6 No. 2 April 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Honey is the natural sweet substance produced by honey bees from the nectar of flowers. Honey composition is very complex, it contains at least 181-200 different substances, hence the enormous benefits of honey within pharmaceutical industry, food, beverage, and aesthetics. Although the demand for honey in Indonesia has been increasing, there is still no honey quality certification labeled on its product. This study used honey from several beekeeping region in Pati regency which has not been certified. The research aims to analyze honey quality based on water content, acidity, and total sugar. The sampling was purposive sampling method in the three different districts in Pati Regency, those were Gembong District, Gunungwungkal District, and Tlogowungu District, because in general this area is the main honey producer as there are many beekeepers and there is still no honey quality information labeled on it. This research used a Completely Randomized Design (CRD) with 3 treatments (locations) 4 replications from different beekeeper in each region. The water content and total sugar used a refractometer and acidity used titration. The research found that there is a significant difference between honey quality from different beekeeping region. The water content is ranged from 24-27,8%, acidity 59,2-68,3ml NaOH/kg, and total sugar 70,6-74,5 oBx. The highest honey quality is obtained from Gembong District with water content 24%, acidity 59,2ml NaOH/kg, and total sugar 74,5oBx.Keywords: Apis mellifera, water content, acidity, total sugar.
PENGARUH PUPUK NANOSILIKA TERHADAP PERTUMBUHAN, UKURAN STOMATA DAN KANDUNGAN KLOROFIL CABAI RAWIT (Capsicum frutescens Linn) VARIETAS CAKRA HIJAU Syella Clarah; Rini Budi Hastuti; Sri Darmanti
Jurnal Akademika Biologi Vol. 6 No. 2 April 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Cayenne pepper is one of horticulture crops that has the benefits and high economic value. Problem often encountered in the cultivation of chili that growth and still low production can not meet consumer demand continues to increase. This causes cayenne prices become relatively expensive in the market. Nutrients silica (Si) role in supporting growth and crop production. Applications use silica has now been developed in the form nanosilica as quickly absorbed by plants and needed in very small amounts. This study aim to assess the effect of nanosilica fertilizer on the growth, size of stomata and content of chlorophyll cayenne pepper. This research was conducted using a Completely Randomized Design (CRD) one factor, that is nanosilica concentration with five treatments: 0 ml/L; 2.5 ml/L; 5 ml/L; 7.5 ml/L; 10 ml/L. The parameters observed are plant height, wet weight, dry weight, number of leaves , size of stomata and content of chlorophyll. The data was analized by Analysis of Variance (ANOVA) and continued with multiple range test Duncan's Multiple Range Test (DMRT) at 95% confidence level. These results indicate that fertilizer nanosilica can increase plant height, wet weight, dry weight, number of leaves  and size of stomata Cayenne pepper. N1-N4 treatment tends to improve content of chlorophyll cayenne pepper although not significant by ANOVA. Keyword: Cayenne pepper, growth, nanosilica, stomata, chlorophyll
ISOLASI DAN KARAKTERISASI KAPANG ENDOFIT DARI PEGAGAN (Centella asiatica (L.) URBAN) Nur Sabrina Hasyyati; Agung Suprihadi; Budi Raharjo; Kristiani Dwiatmi
Jurnal Akademika Biologi Vol. 6 No. 2 April 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

        Endophytic mold live in colonies in healthy plant tissues without causing damage to the host plant. Various types of plants can be potentially as a source of endophytic mold isolates. Plants that are hosts for the mold endophyte one of them is gotu kola (Centella asiatica (L.) Urban). This study aimed to isolate and identify the molecular of endophytic molds from gotu kola. Isolation of endophytic mold through the stages namely stage surface sterilization and purification. Mold isolates obtained later in the identification of the molecular basis using ITS region. DNA amplification using the primers ITS ITS 4 and 5. Ribbon successfully amplified DNA with a size of 600 bp. The results of molecular identification and phylogenetic analysis based BLAST. The results of the analysis of BLAST showed similarities each - each isolates with species of mold on genebank known isolates BiogenCC M1 and BiogenCC M25 with Phoma sp., Isolate BiogenCC M6 with    Colletotrichum siamense, isolates BiogenCC M10 is Colletotrichum destructivum, isolates BiogenCC M15 and BiogenCC M28 with Mycoleptodiscus indiscus, and isolates BiogenCC BiogenCC M18 and M19 with Fusarium oxyporum. Keywords: Mold endophyte, Moleculer identification, ITS, Pegagan.
IDENTIFIKASI ISOLAT MONASCUS SP. HASIL ISOLASI ANGKAK BERDASARKAN GEN INTERNAL TRANSCRIBED SPACER (ITS) DAN PENGUKURAN KANDUNGAN PIGMEN Mia Tri Wardani; Endang Kusdiyantini; Anto Budiharjo
Jurnal Akademika Biologi Vol. 6 No. 2 April 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

The Identification of Monascus based on their morphological characteristic, this methods is very problematic due to several  specimens share similarity on morphology and colours. Nowadays, the identification of molecular based organisms has been done to complement morphological identification, for example mold identification using the Internal Transcribed Spacer (ITS) gene. The objectives of study were to identify of Monascus isolates the result of angkak isolation using Internal Transcribed Spacer (ITS) gene, phylogenetic analyzing,  determination cell growth and determination of production pigment content. Sequences of  ITS Monascus were amplified using PCR and the molecular phylogenetic  analyses was using Neighbor Joining (NJ) method. Based on homology search by Basic Local Alignment Search Tool (BLAST) program and phylogenetic tree analyses, mold of isolates were identified as the Monascus purpureus (100%). The amplified DNA fragments were about 553 bp. Determination of Monascus mold cell growth were carried out by the method of dry weight cell. Determination of pigment by using spectrophotometer at 390 nm wavelength for yellow pigment and 500 nm for red pigment. The result of determination of Monascus mold pigment content show that have increased during fermentation time until day 13. The amount of yellow extracellular pigment production is 37.358 U / g; While the red color pigment of 2.6545 U / g. The amount of red intracellular pigment production is 7.4175 U / g, while the yellow pigment is 30.176 U / g.Keywords: Monascus, angkak, molecular phylogenetic, pigment
PENGARUH PUPUK NANOSILIKA TERHADAP PERTUMBUHAN DAN PRODUKSI TANAMAN KAPAS (GOSSYPIUM HIRSUTUM L. VAR. KANESIA 8) Atin Temon Sari; Sri Widodo Agung Suedy; Sri Haryanti
Jurnal Akademika Biologi Vol. 6 No. 2 April 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Cotton (Gossypium hirsutum L.) is one of agricultural commodity which produces natural fiber as a raw material for the textile industry has a lot of constraints in its cultivation which not optimal, so the improvement of cultivation techniques by performing a balanced fertilizer can be done. Indonesian agricultural soil generally has low fertility rate and silica availability, so silica fertilization would be necessary. Silica causes leaves and stems of plant become upright so the photosynthesis will increased. Nanosilica that contains very small elements of silica which makes it easily absorbed by plants in order to increase growth and yield of cotton. This research aims to determine the effect of nanosilica fertilizer to the growth and production and also to know the effective amount of nanosilica concentration which will increase it of cotton Kanesia 8. The study design was using RAL with 5 different treatments of nanosilica fertilizer amount: 0 ml/L, 2.2 ml/L, 4.2 ml/L, 6.2 ml/L dan 8.2 ml/L with 5 repetitions. The data analysis was using ANOVA and if there is a significantly different then the analysis followed by further test of Duncan's Multiple Range Test (DMRT) at 95% of significance level. The results showed that nanosilica fertilizer with a concentration 2.2 ml/L increased the plant growth mainly at fresh weight of plant by 74.62% and dry weight of plant by 33.66% and it also increased the production of plant mainly in the percentage of fruit changed into cotton by 19.71% and dry weight of cotton by 0.98% than the control treatment.Keywords: nanosilica, growth, production, Gossypium hirsutum
KEANEKARAGAMAN JENISTUMBUHAN PAKU (PTERIDOPHYTA) DI JALUR PENDAKIAN SELO KAWASAN TAMAN NASIONAL GUNUNG MERBABU, JAWA TENGAH Fitri Kusuma Astuti; M Murningsih; J Jumari
Jurnal Akademika Biologi Vol. 6 No. 2 April 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Indonesia has a very high biodiversity, as well as ferns (Pteridophyta).Selo climbing lane at Merbabu Montain Nasional Park selected as the study site because of the many ferns that were founded in this area.The purpose of this study was to analyze the diversity of ferns species.in tropical rain forest ecosystem of high mountains and sub-alpine forests ecosystem on Selo climbing lane at Merbabu Mount National Park. There are many human activity in Selo climbing lanesupposed to influence the existing plants. This research was held on August-December 2016. The method usedinventory inSelo climbing lane and Plot, then analyzed using diversity index. The results found 33 species of ferns from 15 families. The diversity of ferns in the tropical rain forest ecosystem of high mountains fall into the medium category except at post II (2412 masl) has low diversity. Diversity in the sub-alpine forest ecosystem can not be calculated. Key word: diversity, fern, Selo climbing lane.

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