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Biodiversitas Journal of Biological Diversity
ISSN : 1412033X     EISSN : 20854722     DOI : https://doi.org/10.13057/biodiv/d230231
The Biodiversitas Journal was first published in 2000 by the Department of Biology, FMNS, Universitas Sebelas Maret, Surakarta, Indonesia, then in 2006 it was co-published by the Society for Indonesian Biodiversity and that department; since 2017 it was also hosted by Smujo. From 2003-2012 it was accredited by DGHE, Ministry of Education, R.I., since 2014 was indexed by Scopus, where DOAJ and Google Scholar was indexed first.
Articles 6,269 Documents
New record and potential spatial distribution of Curcuma sumatrana (Zingiberaceae): An endemic wild turmeric in Sumatra, Indonesia FARADILA SYAFIRA; NURAINAS NURAINAS; SYAMSUARDI SYAMSUARDI
Biodiversitas Journal of Biological Diversity Vol. 25 No. 11 (2024)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d251112

Abstract

Abstract. Syafira F, Nurainas, Syamsuardi. 2024. New record and potential spatial distribution of Curcuma sumatrana (Zingiberaceae): An endemic wild turmeric in Sumatra, Indonesia. Biodiversitas 25: 4127-4138. Curcuma sumatrana Miq. is a Sumatran turmeric species with medicinal potential. However, it remains underutilized and is classified as vulnerable by the IUCN. Its vulnerability is aggravated by limited knowledge of its distribution, a need for more data on habitat preferences, and habitat degradation. Field observations revealed significant morphological variations among populations, likely influenced by environmental factors. This study assessed the morphology, microhabitat preferences, and spatial distribution of C. sumatrana in West Sumatra, Indonesia. Surveys and laboratory observations highlighted morphological traits, such as leaf and ligule length and width, significantly contribute to these observed variations. Notably, the leaf shape differed between open and shaded areas, being narrowly elliptic in open areas and broadly elliptic in shaded areas. Populations in Koto Malintang and Lubuk Minturun showed distinct differences, separated by six morphological traits. The C. sumatrana prefers habitats with fertile soils, moderate plant diversity, and open land cover, often coexisting with species like Dendrocnide stimulans (L.fil.) Chew, Macaranga tanarius (L.) Müll.Arg., and Diplazium sp. Maximum Entropy modeling (AUC 0.944) predicted a highly suitable habitat of 918 hectares in the western Bukit Barisan range. The model suggests distribution is influenced by soil type and precipitation patterns during the seasonal, warmest, and coldest quarters, as well as land cover. New records from West Sumatra extend the species' known range, reaffirming its vulnerable status with a potential risk of becoming endangered.
Diversity of Ceratocystis fimbriata causing canker and wilt disease on Cupressus sempervirens (Italian cypress) in Indonesia RAHMAT PRATAMA; SUWANDI SUWANDI; AHMAD MUSLIM; MULAWARMAN MULAWARMAN
Biodiversitas Journal of Biological Diversity Vol. 26 No. 1 (2025)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d260128

Abstract

Abstract. Pratama R, Suwandi S, Muslim A, Mulawarman. 2025. Diversity of Ceratocystis fimbriata causing canker and wilt disease on Cupressus sempervirens (Italian cypress) in Indonesia. Biodiversitas 26: 278-287. In 2022-2023, new diseases were observed on Cupressus sempervirens in South Sumatra, Indonesia, with the disease incidence increasing from 17.6% to 26.5% in 2023. Initial symptoms, included stem cankers, black lesions on sapwood and vascular tissue, discoloration and partial wilting of leaves, and eventual complete drying, leading to plant death. The objective of this study was to isolate and identify the fungal pathogen causing wilt disease in C. sempervirens trees using morphological characterization and DNA sequencing. In 2022-2023, a disease survey was conducted in six districts of South Sumatra. The results showed that six out of ten locations were infected, with disease incidence ranging from 4.1% to 17.6% in 2022, increasing to 2% to 26.5% in 2023. Pathogen identification employed a polyphasic approach, combining morphological and molecular characteristics from specific genomic regions (the internal transcribed spacer (ITS) and ?-tubulin). Both the morphological features (including a globose base with a long neck-ended tip with ostiolar hyphae, cylindrical conidia, and hat-shaped ascospores) and phylogenetic analysis identified the isolates as Ceratocystis fimbriata. ITS gene sequences indicated that all the isolates belonged to the ITS5 haplotype. In pathogenicity test, pathogen caused mortality in C. sempervirens, Acacia mangium, and Artocarpus heterophyllus plants. The implications of these findings are significant, as they can potentially lead to the development of effective control measures. To our knowledge, this is the first report of Ceratocystis spp. causing wilt disease on C. sempervirens in South Sumatra.
Impacts of climate change on forest restoration dynamics in the lower montane forest of Doi Suthep-Pui National Park, Northern Thailand SATHID THINKAMPHEANG; TOHRU NAKASHIZUKA; WARONG SUKSAVATE; PANIDA KACHINA; SUTHEERA HERMHUK; LAMTHAI ASANOK; WONGSATORN PHUMPHUANG; BHUPICHIT CHOUIBUMROONG; JIANPING WU; HIROKO KUROKAWA; DOKRAK MAROD
Biodiversitas Journal of Biological Diversity Vol. 25 No. 12 (2024)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d251219

Abstract

Abstract. Thinkampheang S, Nakashizuka T, Suksavate W, Kachina P, Hermhuk S, Asanok L, Phumphuang W, Chouibumroong B, Wu J, Kurokawa H, Marod D. 2024. Impacts of climate change on forest restoration dynamics in the lower montane forest of Doi Suthep-Pui National Park, Northern Thailand. Biodiversitas 25: 4829-4845. Lower montane forest ecosystems are rich in biodiversity but prone to anthropogenic and natural disturbances. Forest restoration aims to emulate natural succession, particularly concerning tree species composition. In this study, we investigated the effects of different forest restoration practices on tree regeneration and the impact of climate change on forest dynamics following restoration in a natural lower montane forest. Three forest dynamics plots (100 m × 100 m) were established in the forest, and two artificial forest restoration approaches were simulated based on mono-species and multi-species plantations, respectively. All trees with a diameter at breast height > 2 cm were tagged, measured, identified, and monitored every 2 years from 2011 to 2021. Forest dynamics, including mortality and recruitment rates, were analyzed in relation to climate change. The results show that recovery varied among restoration practices. The mono-species plantation had the highest species diversity (114 species), followed by the natural forest and multi-species plantation. The mean mortality rate was higher than the net mean recruitment rate in mono-species and multi-species plantations, with significant variation in forest dynamics across species and periods. These fluctuations were closely linked to environmental changes, particularly droughts related to El Niño. Environmental factors exert a substantial influence on forest structure and species composition. In particular, the interplay between forest dynamics and ecological changes underscores the susceptibility of certain species to drought. Our findings imply that multi-species planting, with its potential to mirror the natural climax state of a forest closely, is a highly effective forest restoration strategy that can inspire and motivate further research and action in this field.
Assessment of fungal contamination in dangke, a traditional cheese product from small-scale producers in Enrekang, South Sulawesi SETIAWAN PUTRA SYAH; MIRNAWATI B. SUDARWANTO; IDWAN SUDIRMAN; ANDI NURUL MUKHLISAH; WENY DWI NINGTYAS; MUHAMMAD IRFAN
Biodiversitas Journal of Biological Diversity Vol. 26 No. 7 (2025)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d260724

Abstract

Abstract. Syah SP, Sudarwanto MB, Sudirman I, Mukhlisah AN, Ningtyas WD, Irfan M. 2025. Assessment of fungal contamination in dangke, a traditional cheese product from small-scale producers in Enrekang, South Sulawesi. Biodiversitas 26: 3324-3333. Dangke is a traditional soft cheese produced in Enrekang, South Sulawesi, Indonesia, using household-scale methods that can pose significant food safety risks due to inadequate hygiene practices. This study assessed the level and diversity of fungal contamination in dangke collected from small-scale producers across Enrekang Regency. A total of 20 samples were collected aseptically and analyzed using the Total Plate Count (TPC) method on Sabouraud Dextrose Agar. The results showed that the average fungal count was 5.2 × 10? CFU/g, which greatly exceeded the maximum acceptable limit for solid dairy products (10 CFU/g) according to Codex Alimentarius guidelines. Phenotypic identification revealed the presence of both yeasts (75%) and molds (25%), with nine fungal genera detected. Candida sp. was the dominant genus (30%), followed by Saccharomyces sp. (16%), Geotrichum sp. (11%), Microsporum sp. (13%), Cladosporium sp. (9%), Penicillium sp. (9%), Aspergillus sp. (6%), Rodotorula sp. (5%), and Fusarium sp. (2%). Several of these genera, such as Aspergillus and Fusarium, are known producers of mycotoxins that pose potential health risks. These findings highlight the urgent need for improved hygiene practices, proper handling, and better storage conditions in traditional dangke production to reduce fungal contamination and ensure product safety for consumers.
Pyocyanin derived from the marine sponge-associated bacterium, Pseudomonas aeruginosa P1.S9, has the potential as antibacterial DOVA KELVIN MESRIAN; RIKA INDRI ASTUTI; MUHAMMAD EKA PRASTYA; ARIS TRI WAHYUDI
Biodiversitas Journal of Biological Diversity Vol. 25 No. 11 (2024)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d251113

Abstract

Abstract. Mesrian DK, Astuti RI, Prastya ME, Wahyudi AT. 2024. Pyocyanin derived from the marine sponge-associated bacterium, Pseudomonas aeruginosa P1.S9, has the potential as antibacterial. Biodiversitas 25: 4139-4147. Sponge-associated bacteria are a prolific source of secondary metabolites. Among them, pyocyanin-producing Pseudomonas aeruginosa is a subject of great interest. Pyocyanin is a blue-green pigment known for its enormous biological activity, one of the most notable being antimicrobial. Therefore, this study was performed to optimize the production, to characterize the chemical structure, and to test the antimicrobial activity of pyocyanin. As the sole isolate used, Pseudomonas aeruginosa P1.S9 provided a fundamental premise for pyocyanin synthesis by revealing the presence of phzM and phzS genes. The proteins generated from these genes were highly compatible with two enzymes involved in the pyocyanin production pathway. During the optimization, the maximum level of pyocyanin produced was 29.057±0.691 µg mL-1. The concentration was obtained using a modified King's A medium incubated at 27°C within four days. To assess its purity, the chemical structure of pyocyanin was confirmed by several spectroscopic techniques including UV-Visible (UV-Vis), Fourier Transform Infrared (FT-IR), and Nuclear Magnetic Resonance (1HNMR). All test results closely resemble purified pyocyanin compared to several prior studies. In terms of antimicrobial activity, pyocyanin was effective against ATCC strains of Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus. Its strongest minimum inhibitory concentration (MIC) was 62.5 µg mL-1 against Bacillus subtilis. Subsequently, the accumulation of reactive oxygen species (ROS) as a major mechanism of pyocyanin antibacterial activity has also been verified. The bacterial pathogens cells treated with pyocyanin displayed a brighter luminescence compared to the control without pyocyanin after the addition of 2',7'-dichlorodihydrofluorescein diacetate (H2DCF-DA). Ultimately, the present work comprehensively characterized pyocyanin's promising producer and antibacterial properties.
Karyotype analysis of Eleutherine bulbosa (Iridaceae) from Kalimantan, Indonesia Noor Aini Habibah; Woro Anindito Sri Tunjung; Meti Indrowati; Alin Liana
Biodiversitas Journal of Biological Diversity Vol. 26 No. 5 (2025)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d260544

Abstract

Abstract. Habibah NA, Tunjung WAS, Indrowati M, Liana A. 2025. Karyotype analysis of Eleutherine bulbosa (Iridaceae) from Kalimantan, Indonesia. Biodiversitas 26: 2486-2494. Eleutherine bulbosa or Dayak onion belongs to the family Iridaceae and is widely distributed in Indonesia. However, research on E. bulbosa, especially for the cytogenetic aspect is poorly reported. This study aimed to analyze karyotype of E. bulbosa chromosomes collected from Kalimantan, Indonesia. Chromosome observation was carried out using a modified squash method. The results showed that E. bulbosa has basic chromosome number of 2n: 12. Although it has the same number of chromosomes, each sample shows a different chromosome formula. Those from East Kalimantan and Central Kalimantan have a chromosome formula of 2n=2x=12m. Meanwhile, samples from South Kalimantan have a chromosome formula of 2n=2x=10m+2sm. In comparison, those from North Kalimantan and West Kalimantan have a chromosome formula of 2n=2x=6m+2t+4sm and of 2n=2x=8m+2sm+2t, respectively. Eleutherine bulbosa was also found to have a bimodal karyotype. The most recent finding of this study is the discovery of sub-metacentric and telocentric chromosome morphologies, which have never been seen in previous studies.
Chemical composition, in vitro rumen profile and methane emission of fermented and non-fermented grass-legume mixtures VITA APRIANI; YULIANRI RIZKI YANZA; WULANSIH DWI ASTUTI; VINCENT NIDERKORN; RIMA S. H. MARTIN; JIDAN RAMADANI; WELAS SRI MULYATI; ANURAGA JAYANEGARA
Biodiversitas Journal of Biological Diversity Vol. 26 No. 2 (2025)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d260236

Abstract

Abstract. Apriani V, Yanza YR, Astuti WD, Niderkorn V, Martin RSH, Ramadani J, Mulyati WS, Jayanegara A. 2025. Chemical composition, in vitro rumen profile and methane emission of fermented and non-fermented grass-legume mixtures. Biodiversitas 26: 869-878. This study aimed to evaluate the impact of several grass-legume mixtures, either fermented or non-fermented, on chemical composition, in vitro rumen fermentation profiles, and gas and methane production kinetics. Seven tropical leguminous plants were investigated, i.e., Indigofera zollingeriana, Calliandra calothyrsus, Clitoria ternatea, Centrosema pubescens, Leucaena leucocephala, Bauhinia purpurea, and Arachis pintoi. Each legume was combined with Pennisetum purpureum (elephant grass) in a 50:50 ratio, prepared in both fresh (non-fermented) and fermented (silage) forms. A completely randomized factorial design was employed, with legume type as the primary factor and fermentation status (non-fermented vs fermented) as the secondary factor (N = 5 replicates per treatment). Results revealed that legume type significantly influenced (P<0.05) In Vitro Dry Matter Digestibility (IVDMD) and In Vitro Organic Matter Digestibility (IVOMD), with I. zollingeriana demonstrating the highest digestibility values. A significant interaction between fermentation and legume type was observed in reducing methane gas production (P<0.05). Both legume inclusion and fermentation effectively lowered methane emissions (P<0.05), with the lowest levels recorded for C. calothyrsus and B. purpurea. The study concluded that incorporating tropical legumes, in both fermented and non-fermented forms, into elephant grass can improve digestibility and mitigate methane emissions, with Indigofera and Calliandra showing the most promising results, respectively.
Phytochemical Analysis and antioxidant activity from Phanera semibifida stem and leaves extracts using LC-MS/MS MEYLA SUHENDRA; BERRY JULIANDI; HUDA SHALAHUDIN DARUSMAN; SITI SADIAH; FITMAWATI FITMAWATI; SRI BUDIARTI; PUJI RIANTI
Biodiversitas Journal of Biological Diversity Vol. 26 No. 1 (2025)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d260149

Abstract

Phanera semibifida is a forest plant that contains metabolites such as flavonoids, terpenoids, saponins, and tannins. Lingga Malay ethnic uses P. semibifida stem as the main ingredient in health potions. Based on this primary evidence, P. semibifida has potential medicinal uses. However, studies on metabolite content and bioactivity screening of the stem and leave extract P. semibifida are still limited. This research aimed to identify the phytochemical profile and  bioactivity screening of the P. semibifida stems and leaf extract. The sample was extracted using 96% ethanol with maceration methods. The chemical content was identified using LC-MS/MS methods. Antioxidant potential was eval__uated with DPPH methods. Based on LC-MS/MS analysis, 26 compounds were identified from stem extract, and 24 compounds were identified from leave extract. The compound originates from seven compound groups: carboxylic acid,  flavonoid, phenolics, polifenol, terpenoid, Tricarboxylic acid, and Humulonoid. Carboxylic acid is the most commonly obtained compound in this research. The compounds identified in the stem extract differ from those in the leaf extract. Quercetin-3'-glucuronide, Apigetrin, and Glucogallin are only found in the stem extract, whereas Salicylic acid is only found in the leaf extract. Many researchers report that the group compound has potential as Antioxidants, antiviral or antimicrobial, anti-vitiligo, anticancer or antitumor, anti-inflammatory, antidepressant, and anti-diabetic. The antiradical activity assay revealed that the stem extract had the highest activity, with an IC50 of 9.091 ppm. The IC50 data indicated that higher quantities of flavonoid components could increase antioxidant activity.
Molecular identification of rhizospheric Actinomycetes from karst ecosystems of Gorontalo, Indonesia, and its seed germination induction capability of Zea mays var. doti YULIANA RETNOWATI; ABUBAKAR SIDIK KATILI; NOVRI YOULA KANDOWANGKO; WAWAN PEMBENGO
Biodiversitas Journal of Biological Diversity Vol. 25 No. 12 (2024)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d251212

Abstract

Abstract. Retnowati Y, Katili AS, Kandowangko NY, Pembengo W. 2024. Molecular identification of rhizospheric Actinomycetes from karst ecosystems of Gorontalo, Indonesia, and its seed germination induction capability of Zea mays var. doti. Biodiversitas 25: 4763-4771. Karst, as an extreme ecosystem, was a source of diverse Actinomycetes with varied biological activities. This study explored the plant-growth-promoting potential of rhizospheric Actinomycetes from the karst ecosystem of Gorontalo, with a focus on inducing seed germination in Zea mays var. doti. Four locations in Gorontalo were selected to explore Actinomycetes, targeting approximately 20 different rhizospheric plant species to isolate these microorganisms. Among the 25 isolates obtained, representing diverse morphological types from 12 rhizospheric plants, eight actinomycete isolates exhibited phosphate-solubilizing activity and produced indole-3-acetic acid (IAA). The 16S rRNA gene sequence analysis showed that approximately 75% of the isolates belonged to the Streptomyces genus, including Streptomyces cavourensis strain KRZm-02, Streptomyces sp. strain KRZm-03, Streptomyces pratensis strain KRLl-01, Streptomyces carpaticus strain KRIt-01, Streptomyces sp. strain KRIt-02, and Streptomyces aquilus strain KRPa-01. Additionally, 12.5% of the isolates were identified as Nocardiopsis alba strain KRZm-01 and Micromonospora sp. strain KRPt-01, respectively. The two isolates with the highest plant-growth-promoting potential, Streptomyces pratensis strain KRLl-01 and Streptomyces carpaticus strain KRIt-01, were further tested for their ability to promote germination of Zea mays var. doti seeds over 7 days. Among the two, Streptomyces carpaticus strain KRIt-01 exhibited the highest germination-inducing potential. Overall, the karst ecosystem of Gorontalo offers a valuable reservoir of biological resources with the potential for Plant-Growth-Promoting Rhizobacteria (PGPR). Further studies on the application of these actinomycete isolates as biofertilizers in agricultural and plantation crops could significantly contribute to improving crop growth and productivity, thereby revolutionizing agricultural practices.
Community status of giant clam (Tridacnidae) on Bira of Seribu Islands, Indonesia RATNA KOMALA; DIANA VIVANTI SIGIT; EKA PUTRI AZRA
Biodiversitas Journal of Biological Diversity Vol. 26 No. 4 (2025)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d260438

Abstract

Abstract. Komala R, Sigit DV, Azrai EP. 2025. Community status of giant clam (Tridacnidae) on Bira of Seribu Islands, Indonesia. Biodiversitas 26: 1891-1897. The Tridacnidae family, commonly referred to as giant clams, has a comparatively high ecological and commercial potential but is vulnerable to environmental shifts and human activities. The study aims to identify species of Tridacnidae, ascertain their abundance, diversity, and dominance, and examine the correlation between environmental factors and the population abundance of giant clams on Bira of Seribu Islands, Indonesia. The location of the observation is determined purposefully based on the coral reef existence. Data collection was carried out using the line Transect method, which involves measuring 10 transects over 100 meters from the shore to the reef edge. The Underwater Photo Transect (UPT) sample collection technique was employed at a depth of between 3 and 4.5 meters. The Mantel test was used to examine the relationship between environmental parameters and the abundance of giant clams. In contrast, the abundance, biodiversity, and dominance index formulas were used to interpret the data. The result identified three species of giant clams from the genera Tridacna and Hippopus, with T. maxima showing the highest abundance, followed by T. gigas and H. hippopus. The diversity encompassed the low to middle criteria with low criteria dominance. Environmental parameters remained within a range favorable for the growth of giant clams, but there is no significant correlation between the environmental parameters with the abundance of Tridacnidae.

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