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kPERHIMPUNAN MIKROBIOLOGI INDONESIA (SeKretariat PERMI), Gedung 10.2 Indonesian Life Sciences Center (ILSC), Zona Bisnis Teknologi Puspiptek, Jalan Raya Serpong - Bogor Gunung Sindur, Jawa Barat 16340, Indonesia. Email: microbiology.indonesia@gmail.com
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Microbiology Indonesia
Published by Perhimpunan Mikrobiologi Indonesia
ISSN : 19783477     EISSN : 20878575     DOI : -
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology
Microbiology Indonesia provides a unique venue for publishing original researches in microbiology (espesially from Indonesian reseachers), and ensures that authors could reach the widest possible audience. Microbiology Indonesia publishes a wide range of research disciplines on bacteria, archaea, fungi, protozoa, and virus as well as biotechnology related to microbiology. Topics include (but are not limited to): -methods in microbiology, -bioprocess, -environmental microbiology, -food microbiology, -plant-microbe interaction, -animal-microbe interactions, -microbial community, -microbial genetics, -virology, -comparative and functional microbial genomics, -and gene expression in microbes.
Arjuna Subject : Imunologi dan Mikrobiologi (semua kategori) - Mikrobiologi dan Bioteknologi Terapan
Articles 5 Documents
 1 
Search results for , issue "Vol. 14 No. 2 (2020): June 2020" : 5 Documents clear
Utilization of Leaves in Mine Reclamation Land as Organic Fertilizer with Effective Bioactivatory of Microorganism 4 (em4) and Molasses Supiana Dian Nurtjahyani; Dwi Oktafitria; Sriwulan Sriwulan; Nova Maulidina; Imas Cintamulya; Eko Purnomo
Microbiology Indonesia Vol. 14 No. 2 (2020): June 2020
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (494.467 KB) | DOI: 10.5454/mi.14.2.5

Abstract

Organic fertilizer as an alternative to reduce the scarcity of chemical fertilizers, otherwise it can improve soil fertility. PT. Semen Gresik - Semen Indonesia Persero (Tbk.) Has a lot of plants in the treatment period in ex-mining land that needs fertilizer to help plant growth. In addition, in post-mining land, there is a lot of organic waste in the form of dry leaves under the stands of reclamation trees. The organic waste has the potential to be processed and used as organic fertilizer. This study aims to determine the utilize leaf litter in the reclaimed mine land as organic fertilizer with EM 4 and Molasses bioactivators. This study used is a laboratory experimental method. The results showed the composition of solid material in the form of leaf litter, bran, cow dung and husk charcoal in a ratio of 2: 1: 1: 1 as well as looking material in the form of molasses and EM4 with a dose of 100ml per 100kg of solid material dissolved in enough water (fertilizer can be clenched and expanded slowly and not broken). Conclusion leaf litter in the former reclaimed land can be processed and used as organic fertilizer.
Antagonistic Effect of Two Indigenous Phosphate Solubilizing Bacteria, Burkholderia contaminans PSB3 and Acinetobacter baumannii PSB11 Isolated From Different Crop Soils RULLY ADI NUGROHO; VINCENTIA IRENE MEITINIARTI; CHRISSEPTINA DAMAYANTI
Microbiology Indonesia Vol. 14 No. 2 (2020): June 2020
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (558.522 KB) | DOI: 10.5454/mi.14.2.1

Abstract

Phosphorus is the most important key element in the nutrition of plants. Although P is abundant in soils, it is a major limiting factor for plant growth as it is in an unavailable form for roots uptake. Phosphate solubilizing bacteria (PSB) has ability to convert insoluble form of P to an available form. This study was aimed at screening and characterizing phosphate-solubilizing bacteria from manure and different rhizosphere and to ascertain a potential benefit to use mixed cultures to improve P solubilization. A total of 12 PSB colonies were isolated on Pikovskaya’s agar medium containing tricalcium phosphate. Out of 12 bacterial isolates, 2 isolates showed high phosphate solubilization index (2.17 and 1.83, respectively) were selected for further study. Based on the 16S rRNA gene sequence analysis, PSB3 was closely related to Burkholderia contaminans (99%), and PSB11 was closely related to Acinetobacter baumannii (99%). The mean P dissolved in liquid cultures of PSB3 and PSB11 in a 14-day incubation were 96.7 and 39.3 mg l-1, respectively. Mixed inoculation of B. contaminans PSB3 and A. baumannii PSB11 could not increase the solubilization activity significantly, suggesting there is antagonistic behavior of one isolate towards another. As the interaction of these two isolates may be antagonistic, co-inoculation of these bacteria for P solubilization is not recommended. However, further study is needed to confirm these results.
Enhanced Ethanol Production by High Temperature-Tolerance Mutant Pichia kudriavzevii T-T2 in various Carbon and Nitrogen Sources Monica Ina Martha; Rika Indri Astuti; Wulan Tri Wahyuni
Microbiology Indonesia Vol. 14 No. 2 (2020): June 2020
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (532.767 KB) | DOI: 10.5454/mi.14.2.3

Abstract

Previously, we had constructed proline accumulating Pichia kudriavzevii yeast mutant (T-T2) that exhibited high temperature tolerance phenotype. In this study we then analyzed the ethanol production of the particular mutant isolate in various carbon (C) and nitrogen sources (N). Fermentation rate of T-T2 either in 4% glucose or substrate mixture (SM = 2%glucose : 2% xylose) was higher than WT strain. The highest CO2 evolving rate by T-T2 in glucose and SM was 57.0 ml/hour and 51.4 ml/hour, respectively, suggesting that T-T2 was capable of using xylose as C-source. T-T2 resulted significant increase of total evolved CO2 than WT strain in glucose as substrate. T-T2 isolate was capable of producing higher ethanol than its WT strains in various C-sources, including glucose, maltose, sucrose, xylose and SM. However, glucose was found as the most preferable C-source to produce ethanol (3.2%) whereas maltose was the least preferable one. T-T2 was found capable in using inorganic sources (NaNO3) to produce ethanol (2.4%) as compared to other N-sources (urea, (NH4)2SO4, NH4Cl). Ethanol production by T-T2 strain in NaNO3 was 16% higher than its WT strain. This data indicate, that NaNO3 is potentially applied as N source alternative to produce ethanol. Based on our study, mutant strain T-T2 shows higher fermentation rate than its WT strain in various sugar and N-sources. The fact that T-T2 could produce ethanol from SM in a similar fermentation rate to single glucose as substrate, suggesting its potential application in the 2nd generation of bioethanol productions.
Synergistic Interaction of Arbuscular Mycorrhizal Fungi and Phosphate-Solubilizing Bacteria with NPK Fertilizer to Improve Sorghum bicolor (L.) Moench Growth under Saline Condition Indriati Ramadhani; Sri Widawati
Microbiology Indonesia Vol. 14 No. 2 (2020): June 2020
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1355.827 KB) | DOI: 10.5454/mi.14.2.4

Abstract

ABSTRACT Sweet sorghum (Sorghum bicolor (L.) Moench) is a food source and a raw material in biofuel-ethanol production. Arbuscular mycorrhizal fungi (AMF) and phosphate-solubilizing bacteria (PSB) are called rhizosphere microorganisms, which are useful microorganisms that enhance plant growth. Rhizosphere microorganisms also increase plant's resistance to environmental stress, while NPK fertilizer used on agricultural land are found to increase crop yields only. However, its continuous application has a negative impact on the environment. Therefore, this research aimed to study the synergy between AMF, and PSB with NPK fertilizer in influencing the sweet sorghum growth in saline condition. Two treatment factors were used, which are microbes combination (arbuscular mycorrhizal fungi and phosphate-solubilizing bacteria), and NPK doses without NPK, 25% NPK, 50% NPK, and 100% NPK. Furthermore, zeolite was used to grow sweet sorghum seeds individually and were maintained for one month in a greenhouse. Pots were watered with 50% seawater (freshwater: seawater = 1:1) every day to keep the moisture. Plant growth parameters were also measured, which includes AMF colonization in the roots, number of AMF spores, and PSB population in the planting medium. The combination of AMF, PSB, and NPK in sweet sorghum increased the plant height, number of leaves, plant fresh weight, plant dry weight, and total of plant P, although not always significant. AMF+PSB+25% NPK produced the highest number in all parameters. Therefore, the synergy between AMF, and PSB with NPK fertilizer is able to increase the plant growth in saline condition. Key words: arbuscular mycorrhizal fungi, phosphate-solubilizing bacteria, NPK, saline, and sweet sorghum ABSTRAK Sorgum manis (Sorghum bicolor (L.) Moench) merupakan sumber makanan dan bahan baku dalam produksi bioetanol. Jamur mikoriza arbuskular (JMA) dan bakteri pelarut fosfat (BPF) disebut sebagai mikroorganisme rizosfer, yang merupakan mikroorganisme yang berguna untuk meningkatkan pertumbuhan tanaman. Mikroorganisme rizosfer juga meningkatkan ketahanan tanaman terhadap cekaman lingkungan, sedangkan pupuk NPK yang digunakan pada lahan pertanian ternyata hanya meningkatkan hasil panen saja. Namun, penerapannya yang berkelanjutan memiliki dampak negatif terhadap lingkungan. Oleh karena itu, penelitian ini bertujuan untuk mempelajari sinergi antara JMA, dan BPF dengan pupuk NPK dalam mempengaruhi pertumbuhan sorgum manis dalam kondisi salin. Dua faktor perlakuan yang digunakan, yaitu kombinasi mikroba (jamur mikoriza arbuskular dan bakteri pelarut fosfat), dan dosis NPK tanpa NPK, 25% NPK, 50% NPK, dan 100% NPK. Selanjutnya, zeolit digunakan untuk menumbuhkan biji sorgum manis secara individu dan dipelihara selama satu bulan di rumah kaca. Pot disiram dengan 50% air laut (air tawar: air laut = 1: 1) setiap hari untuk menjaga kelembaban. Parameter pertumbuhan tanaman juga diukur, yang meliputi kolonisasi JMA di akar, jumlah spora JMA, dan populasi BPF dalam media tanam. Kombinasi JMA, BPF, dan NPK dalam sorgum manis meningkatkan tinggi tanaman, jumlah daun, berat basah tanaman, berat kering tanaman, dan P total tanaman, meskipun tidak selalu signifikan. JMA + BPF + 25% NPK menghasilkan nilai tertinggi di semua parameter pertumbuhan. Oleh karena itu, sinergi antara JMA dan BPF dengan pupuk NPK mampu meningkatkan pertumbuhan tanaman dalam kondisi salin. Kata kunci: jamur mikoriza arbuskular, bakteri pelarut fosfat, NPK, salin, dan sorgum manis
In Silico Study on Testing Antidiabetic Compounds Candidate from Azaphilone Mold Monascus sp. Anna Yuliana; Hilman Fitriaji S P; Khofi Siti Mukhaufillah; Lina Rahmawati Rizkuloh
Microbiology Indonesia Vol. 14 No. 2 (2020): June 2020
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1556.808 KB) | DOI: 10.5454/mi.14.2.2

Abstract

Monascus sp. is a mold that is used for rice fermentation so that the brown rice is produced (Red Mold Rice) or Angkak. This rice has long been used on Asian food and traditional medicine. Angkak contains many bioactive compounds, including monakolin which is potentially to be used as nutraceutical. Monascus sp. produced pigments include yellow (Ankaflavin, Monaskin), Orange (Rubropunktatin, Monaskorubrin) and Red (Rubropunktamin, monaskorubramin) which have various biological activities. The purpose of the study was to know the pigments of the Monascus sp. as a compound antidiabetic candidate. This includes the Azaphilone Kapang Monascus sp. derivative compound In Silico. The test compound consists of 57 compounds of Monascus sp. Glycogen Fosforilase used as an antidiabetic receptor. Ligan preparation is drawn using ChemDraw software then with Marvin Sketch done Protonasi. Testing Drug Scan (Screening ligand based Drug likeness). The preparation of receptors by downloading antidiabetic receptors in Protein Data Bank (GDP). The ADME study was conducted with PreADMET web-based software. Validate the docking method and molecular tethering using the Autodock software 4.2.6. Drugscan test results on compounds Isolate MPs4 have qualified grades in all parameters such as molecular weight, proton donor, proton acceptor, log p and molar refractory. The results of the ADME test in the compound Isolate MPs4 have a qualified value in all parameters Caco-2, HIA (Human Intestinal Absorption), as well as in the PPB (Protein Plasma Binding). The result of the docking test in the Isolate compound MPs4 to be the best and qualified compound because it has a smaller affinity binding than the natural ligands and the comparator ligand (glibenclamide). The result of this research Isolate MPs4 is the candidate for new drugs antidiabetic. Keyword : Monascus sp., In silico, Azaphilone, Docking, Antidiabetic

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