cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
Iman Rusmana
Contact Email
rusmana13@yahoo.com
Phone
+62217560536
Journal Mail Official
microbiology.indonesia@gmail.com
Editorial Address
kPERHIMPUNAN MIKROBIOLOGI INDONESIA (SeKretariat PERMI), Gedung 10.2 Indonesian Life Sciences Center (ILSC), Zona Bisnis Teknologi Puspiptek, Jalan Raya Serpong - Bogor Gunung Sindur, Jawa Barat 16340, Indonesia. Email: microbiology.indonesia@gmail.com
Location
Kota tangerang,
Banten
INDONESIA
Microbiology Indonesia
Published by Perhimpunan Mikrobiologi Indonesia
ISSN : 19783477     EISSN : 20878575     DOI : -
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology
Microbiology Indonesia provides a unique venue for publishing original researches in microbiology (espesially from Indonesian reseachers), and ensures that authors could reach the widest possible audience. Microbiology Indonesia publishes a wide range of research disciplines on bacteria, archaea, fungi, protozoa, and virus as well as biotechnology related to microbiology. Topics include (but are not limited to): -methods in microbiology, -bioprocess, -environmental microbiology, -food microbiology, -plant-microbe interaction, -animal-microbe interactions, -microbial community, -microbial genetics, -virology, -comparative and functional microbial genomics, -and gene expression in microbes.
Arjuna Subject : Imunologi dan Mikrobiologi (semua kategori) - Mikrobiologi dan Bioteknologi Terapan
Articles 9 Documents
 1 
Search results for , issue "Vol. 17 No. 1 (2023): March" : 9 Documents clear
Distribution of Rhizopheric actinomycetes on Karst Ecosystem of Gorontalo, Indonesia Ledy Mutmainah Y Syahril; Wirnangsi D Uno; Abubakar Sidik Katili; Yuliana Retnowati
Microbiology Indonesia Vol. 17 No. 1 (2023): March
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5454/mi.17.1.24-30

Abstract

Karst ecosystem is one type of extreme marginal soil with low chemical, physical and biological fertility. Karst soil is characterized by high calcium (Ca) content, which can affect the availability of essential elements for plants. Actinomycetes are the pioneers of phosphor-poor soil and are distributed on various types of soil, including Karst soil. Distribution, abundance, and diversity of actinomycetes on plant rhizosphere are strongly influenced by soil physicochemical characteristics. The objective of this study was to obtain the actinomycetes and to determine the abundance and distribution in the rhizosphere of various types of plants in the karst ecosystems of Gorontalo. The soil sampling was conducted on the plant's rhizosphere on Bilato, Karang Putih, and Biluhu Karst Hill of Gorontalo based on the purposive sampling method. The physicochemical analysis included humidity, temperature, and soil acidity. The results showed that actinomycetes were found on various plant rhizospheres with an abundance of about 3x102 to 1x104 CFUmL-1. There were five actinomycetes isolated successfully on the plant rhizosphere.The AB-01 isolate was specifically found on Ficus sp. and Scizium sp, the ABT-01 isolates were just distributed on Plumeria abusta, Leucaena leocepala, and Monrngaceae, whereas AKP-01, AKP-02, and AB- 02 were distributed on almost of plant rhizosphere.
Endophytic Bacteria of Pemphis acidula on Karst Ecosystem of Gorontalo, Indonesia Yuliana Retnowati; Dewi Wahyuni K Baderan; Ramli Utina
Microbiology Indonesia Vol. 17 No. 1 (2023): March
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (11102.1 KB) | DOI: 10.5454/mi.17.1.18-24

Abstract

Wild plant Pemphis acidula in coastal habitats is often associated with microbes on their root, stem, or leaf. The objective of the study was to reveal the endophytic bacteria associated with Pemphis acidula in the coastal area of Gorontalo, the study antibacterial activity of endophytic bacteria against pathogenic bacteria, and identify endophytic bacteria based on molecular characteristics. The Pemphis acidula sampling was conducted on three coastal areas of Gorontalo, including Biluhu beach, Dulanga beach, and Olele beach. The sample consists of the root, stem, and leaf of Pemphis acidula. The result showed that the endophytic bacteria were just found on the leaf. There were four isolates with similar morphological features. The antibacterial activity on a broad spectrum of two endophytic bacteria against both Escherichia coli and Staphylococcus aureus, whereas two isolates others on a narrow spectrum against Staphylococcus aureus. The endophytic bacterial identify as Bacillus tequilensis based on 16S rRNA gene sequence and this isolate is closely related to Bacillus tequilensis strain 20Q9-B-4-13 OK653944.1.37-1458 on a similarity index of 100%.
Effect of Cocoa Bean Fermentation Using Lactic Acid Bacteria and Yeast Starters on Flavonoid Formation and Antioxidant Activity Anja Meryandini; Irvan Anwar; Titi Candra Sunarti
Microbiology Indonesia Vol. 17 No. 1 (2023): March
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (365.174 KB) | DOI: 10.5454/mi.17.1.1-8

Abstract

This study investigates the effect of fermentation using lactic acid bacteria and yeast as starters on the formation of flavonoid compounds and the antioxidant activity of cacao beans. The fermentation process were divided into 4 groups: F1: spontaneous fermentation, F2: fermentation using Lactic Acid Bacteria (LAB), F3: fermentation using yeast and F4: fermentation using LAB and yeast. The extraction process was done using ethanol. Flavonoid content was analysis using spectrophotometer assay. The antioxidant activity was analyzed by 1,1-difenil-2-pikrilhidrazil (DPPH) method. All ethanol extract samples of fermented cacao beans contained alkaloids, polyphenols, flavonoids, and tannins. The flavonoid compounds from ethanol extract of cacao beans in F1 is 4.35 ± 0.20 mg/L, F2 (5.64 ± 0.05), F3 (5.37 ± 0.17), and F4 (5.99 ± 0.23 mg/L). The antioxidant activity of cacao bean fermentation extracts using starter were increase compared to the spontaneous fermentation extract (F1). The antioxidant activity in F2 increased to 46.45 ± 2.00%, F3 (49.05 ± 0.58%), and F4 (50.33 ± 0.43%), while the antioxidant activity of F1 was 42.31 ± 0.66%. IC50 value as the ability of the extract to reduce 50% DPPH radical on the ethanol extract of cacao beans from spontaneous fermentation (F1) was 141.67 mg/L. The IC50 value of the fermented cacao bean extract with the addition of starter was obtained at F2 at 109.30 mg/L, F3 (97.51), and F4 is 88.15 mg/L.
Rapid Detection of Foodborne Pathogen Bacteria Vibrio parahaemolyticus in Seafood Using Gene ToxR with Real-Time Polymerase Chain Reaction Method Ismaya Krisdawati; Muktiningsih Nurjayadi; Jefferson Lynford Declan; Gladys Indira Putri; Dandy Akbar Juliansyah; Maharani Azka Azzahra; Irvan Maulana; Irma Ratna Kartika; Vira Saamia; Dwi Ana Oktaviani; I Made Wiranatha; Hesham Ali Al-Enshashy
Microbiology Indonesia Vol. 17 No. 1 (2023): March
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1613.88 KB)

Abstract

Cases of food poisoning often occur due to food contamination caused by pathogenic bacteria. One of the pathogenic bacteria is Vibrio parahaemolyticus which is found in seafood. Thus, a fast, accurate and specific detection method is needed. The purpose of this study was to quickly detect Vibrio parahaemolyticus bacteria in seafood samples targeting the ToxR gene using Real Time PCR. In a previous study, gradient PCR was used to optimize ideal annealing temperature ranges from 53-62°C and revealed that 58°C produced the best outcomes for the ToxR primer with a size of 171 base pairs. Real-Time PCR was utilized to amplify, specify, and test for sensitivity under the ideal conditions from the PCR Gradient. The confirmation results show that the primer pairs could amplify ToxR of Vibrio parahaemolyticus with the amount of concentration as much as 50 ng/µL with Ct 10,69 and 10,32 and melting curve at temperature 82,18°C and 82,23°C. This primer pair can also distinguish non-target bacteria with different Ct and melting curve temperature. The sensitivity assay for this primer can amplify DNA templates at concentration 0,0032 ng/µL. Shrimp samples that are contaminated artificially can still be detected at Ct 13,02 and Ct 13,09. Based on these results, it can be concluded that Real Time PCR with ToxR primer can be applied to develop a detection kit for Vibrio parahaemolyticus in seafood.
Combination of Pseudomonas fluorescens and Liquid Organic Fertilizer on Growth and Production of Peanut (Arachis hypogaea L.) Listy Anggraeni; Desi Ribut Saputri; . Damanhuri; Tirto Wahyu Widodo; . Jumiatun; . Handoko; Diding Rachmawati
Microbiology Indonesia Vol. 17 No. 1 (2023): March
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (10587.623 KB) | DOI: 10.5454/mi.17.1.25-30

Abstract

Excessive and continuous use of synthetic chemicals results in a decrease in soil fertility, thereby reducing peanut production. Minimizing the use of synthetic chemicals can be done through the application of liquid organic fertilizers. This study aims to examine the use of Pseudomonas fluorescens and liquid organic fertilizer in the growth and production of peanuts (Arachis hypogaea L.). This research uses a factorial RBD with two factors. The first factor was the concentration of P. fluorescens, which consisted of 0 ml.l-1, 10 ml.l-1, 15 ml.l-1, and 20 ml.l-1. The second factor was the concentration of liquid organic fertilizer, consisting of 0 ml.l-1, 100 ml.l-1, and 250 ml.l-1. The results showed that the P. fluorescens 15 ml.l-1 treatment showed significant results in stem diameter (6.03 mm), number of root nodules (141.83), fresh biomass weight (355.97 g), dry biomass weight (75.87 g), fresh pod weight (62.39 g), dry pod weight (37.00 g), the total number of pods (25.30), and number of pithy pods (20.51). In the treatment of liquid organic fertilizer, 100 ml.l-1 showed significant results in fresh biomass weight (315.69 g), dry biomass weight (66.97 g), fresh pod weight (55.21 g), dry pod weight (32.82 g), number of pithy pods (17.60), and seed weight per sample with an average production yield of 17.01 g/sample (2.7 tons/ha). The use of P. fluorescens and liquid organic fertilizer simultaneously showed no interaction with all observed variables.
Effect of Cocoa Bean Fermentation Using Lactic Acid Bacteria and Yeast Starters on Flavonoid Formation and Antioxidant Activity Anja Meryandini; Irvan Anwar; Titi Candra Sunarti
Microbiology Indonesia Vol. 17 No. 1 (2023): March
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5454/mi.17.1.7-14

Abstract

This study investigates the effect of fermentation using lactic acid bacteria and yeast as starters on the formation of flavonoid compounds and the antioxidant activity of cacao beans. The fermentation process were divided into 4 groups: F1: spontaneous fermentation, F2: fermentation using Lactic Acid Bacteria (LAB), F3: fermentation using yeast and F4: fermentation using LAB and yeast. The extraction process was done using ethanol. Flavonoid content was analysis using spectrophotometer assay. The antioxidant activity was analyzed by 1,1-difenil-2-pikrilhidrazil (DPPH) method. All ethanol extract samples of fermented cacao beans contained alkaloids, polyphenols, flavonoids, and tannins. The flavonoid compounds from ethanol extract of cacao beans in F1 is 4.35 ± 0.20 mg L-1, F2 (5.64 ± 0.05), F3 (5.37 ± 0.17), and F4 (5.99 ± 0.23 mg L-1). The antioxidant activity of cacao bean fermentation extracts using starter were increase compared to the spontaneous fermentation extract (F1). The antioxidant activity in F2 increased to 46.45 ± 2.00%, F3 (49.05 ± 0.58%), and F4 (50.33 ± 0.43%), while the antioxidant activity of F1 was 42.31 ± 0.66%. IC50 value as the ability of the extract to reduce 50% DPPH radical on the ethanol extract of cacao beans from spontaneous fermentation (F1) was 141.67 mg L-1. The IC50 value of the fermented cacao bean extract with the addition of starter was obtained at F2 at 109.30 mg L-1, F3 (97.51), and F4 is 88.15 mg L-1.
Rapid Detection of Foodborne Pathogen Bacteria Vibrio parahaemolyticus in Seafood using Gene ToxR with Real-Time Polymerase Chain Reaction Method Ismaya Krisdawati; Muktiningsih Nurjayadi; Jefferson Lynford Decan
Microbiology Indonesia Vol. 17 No. 1 (2023): March
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5454/mi.17.1.15-23

Abstract

Cases of food poisoning often occur due to food contamination caused by pathogenic bacteria. One of the pathogenic bacteria is Vibrio parahaemolyticus which is found in seafood. Thus, a fast, accurate and specific detection method is needed. The purpose of this study was to quickly detect Vibrio parahaemolyticus bacteria in seafood samples targeting the ToxR gene using Real Time PCR. In a previous study, gradient PCR was used to optimize ideal annealing temperature ranges from 53-62°C and revealed that 58°C produced the best outcomes for the ToxR primer with a size of 171 base pairs. Real-Time PCR was utilized to amplify, specify, and test for sensitivity under the ideal conditions from the PCR Gradient. The confirmation results show that the primer pairs could amplify ToxR of Vibrio parahaemolyticus with the amount of concentration as much as 50 ng ?L-1 with Ct 10.69 and 10.32 and melting curve at temperature 82.18°C and 82.23°C. This primer pair can also distinguish non- target bacteria with different Ct and melting curve temperature. The sensitivity assay for this primer can amplify DNA templates at concentration 0.0032 ng ?L-1. Shrimp samples that are contaminated artificially can still be detected at Ct 13.02 and Ct 13.09. Based on these results, it can be concluded that Real Time PCR with ToxR primer can be applied to develop a detection kit for Vibrio parahaemolyticus in seafood.
Combination of Pseudomonas fluorescens and Liquid Organic Fertilizer on Growth and Production of Peanut (Arachis hypogaea L.) Listy Anggraeni
Microbiology Indonesia Vol. 17 No. 1 (2023): March
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5454/mi.17.1.31-36

Abstract

Excessive and continuous use of synthetic chemicals results in a decrease in soil fertility, thereby reducing peanut production. Minimizing the use of synthetic chemicals can be done through the application of liquid organic fertilizers. This study aims to examine the use of Pseudomonas fluorescens and liquid organic fertilizer in the growth and production of peanuts (Arachis hypogaea L.). This research uses a factorial RBD with two factors. The first factor was the concentration of P. fluorescens, which consisted of 0 ml.l-1, 10 ml.l-1, 15 ml.l-1, and 20 ml.l-1. The second factor was the concentration of liquid organic fertilizer, consisting of 0 ml.l-1, 100 ml.l-1, and 250 ml.l-1. The results showed that the P. fluorescens 15 ml.l-1 treatment showed significant results in stem diameter (6.03 mm), number of root nodules (141.83), fresh biomass weight (355.97 g), dry biomass weight (75.87 g), fresh pod weight (62.39 g), dry pod weight (37.00 g), the total number of pods (25.30), and number of pithy pods (20.51). In the treatment of liquid organic fertilizer, 100 ml.l-1 showed significant results in fresh biomass weight (315.69 g), dry biomass weight (66.97 g), fresh pod weight (55.21 g), dry pod weight (32.82 g), number of pithy pods (17.60), and seed weight per sample with an average production yield of 17.01 g/sample (2.7 tons/ha). The use of P. fluorescens and liquid organic fertilizer simultaneously showed no interaction with all observed variables.
Diversity of Bacterial Phenol Hydroxylase-encoding Genes from Fuel- contaminated Silt Soil Suraj Rajan Vasandani
Microbiology Indonesia Vol. 17 No. 1 (2023): March
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5454/mi.17.1.1

Abstract

Phenol is an aromatic compound often used as a raw material or intermediate in various industries. Improper handling and disposal may lead to the accumulation of this hazardous compound. Bioremediation is the most viable method to remove phenol from contaminated environment. In this study, the diversity of genes that encode for phenol hydroxylase, a key enzyme in phenol degradation, was assessed in gasoline-contaminated soil in a commercial gas station in Central Jakarta, Indonesia. Partial phenol hydroxylase-encoding gene library was constructed using a pair of universal primer in the pGEM-Teasy vector. A total of 30 recombinant clones were obtained and sequenced to analyze the genetic diversity of this gene. Obtained clones were 86–99% identical to phenol hydroxylase-related proteins. Phylogenetic tree analysis on amino acid sequences derived from our library revealed that 56.67% of the cloned fragments were closely related to Proteobacteria, while 20% of the clones were clustered with Actinobacteria. The rest 23.33% of the clones formed a cluster separate from any of the reference sequences, possibly indicating the presence of novel phenol hydroxylase genes.

Page 1 of 1 | Total Record : 9

 1 

Filter by Year

2023 2023


Reset
Filter By Issues
All Issue Vol. 17 No. 2 (2023): June Vol. 17 No. 1 (2023): March Vol. 16 No. 2 (2022): December Vol. 16 No. 1 (2022): March Vol. 15 No. 3 (2021): September 2021 Vol. 15 No. 2 (2021): June 2021 Vol. 15 No. 1 (2021): March 2021 Vol. 15 No. 4 (2021): December Vol. 14 No. 4 (2020): December 2020 Vol. 14 No. 3 (2020): September 2020 Vol. 14 No. 2 (2020): June 2020 Vol. 14 No. 1 (2020): March 2020 Vol. 13 No. 4 (2019): December 2019 Vol. 13 No. 3 (2019): September 2019 Vol. 13 No. 2 (2019): June 2019 Vol. 13 No. 1 (2019): March 2019 Vol. 12 No. 3 (2018): September 2018 Vol. 12 No. 2 (2018): June 2018 Vol. 12 No. 1 (2018): March 2018 Vol. 11 No. 4 (2017): December 2017 Vol. 11 No. 3 (2017): September 2017 Vol. 11 No. 2 (2017): Juni 2017 Vol. 11 No. 1 (2017): March 2017 Vol. 10 No. 4 (2016): December 2016 Vol. 10 No. 3 (2016): September 2016 Vol. 10 No. 2 (2016): June 2016 Vol. 10 No. 1 (2016): March 2016 Vol. 9 No. 4 (2015): December 2015 Vol. 9 No. 3 (2015): September 2015 Vol. 9 No. 2 (2015): June 2015 Vol. 9 No. 1 (2015): March 2015 Vol. 8 No. 4 (2014): December 2014 Vol. 8 No. 3 (2014): September 2014 Vol. 8 No. 2 (2014): June 2014 Vol. 8 No. 1 (2014): March 2014 Vol. 7 No. 4 (2013): November 2013 Vol. 7 No. 3 (2013): September 2013 Vol. 7 No. 2 (2013): June 2013 Vol. 7 No. 1 (2013): March 2013 Vol. 6 No. 4 (2012): December 2012 Vol. 6 No. 3 (2012): September 2012 Vol. 6 No. 2 (2012): June 2012 Vol. 6 No. 1 (2012): March 2012 Vol. 5 No. 4 (2011): December 2011 Vol. 5 No. 3 (2011): September 2011 Vol. 5 No. 2 (2011): June 2011 Vol. 5 No. 1 (2011): March 2011 Vol. 4 No. 3 (2010): December 2010 Vol. 4 No. 2 (2010): August 2010 Vol. 4 No. 1 (2010): April 2010 Vol. 3 No. 3 (2009): December 2009 Vol. 3 No. 2 (2009): August 2009 Vol. 3 No. 1 (2009): April 2009 Vol. 2 No. 3 (2008): December 2008 Vol. 2 No. 2 (2008): August 2008 Vol. 2 No. 1 (2008): April 2008 Vol. 1 No. 3 (2007): December 2007 Vol. 1 No. 2 (2007): August 2007 Vol. 1 No. 1 (2007): April 2007 More Issue