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Jefferson Lynford Declan
(1) Department Of Chemistry, Faculty Of Mathematics And Natural Science, Universitas Negeri Jakarta, Gedung KH. Hasjim Asj'ari, 6th Floor, Jl. Rawamangun Muka, Jakarta Timur, 13220, Indonesia (2) Research Center For Detection Of Pathogenic Bacteria,
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Public Health Social Sciences Other

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Rapid Detection of Foodborne Pathogen Bacteria Vibrio parahaemolyticus in Seafood Using Gene ToxR with Real-Time Polymerase Chain Reaction Method Ismaya Krisdawati; Muktiningsih Nurjayadi; Jefferson Lynford Declan; Gladys Indira Putri; Dandy Akbar Juliansyah; Maharani Azka Azzahra; Irvan Maulana; Irma Ratna Kartika; Vira Saamia; Dwi Ana Oktaviani; I Made Wiranatha; Hesham Ali Al-Enshashy
Microbiology Indonesia Vol. 17 No. 1 (2023): March
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1613.88 KB)

Abstract

Cases of food poisoning often occur due to food contamination caused by pathogenic bacteria. One of the pathogenic bacteria is Vibrio parahaemolyticus which is found in seafood. Thus, a fast, accurate and specific detection method is needed. The purpose of this study was to quickly detect Vibrio parahaemolyticus bacteria in seafood samples targeting the ToxR gene using Real Time PCR. In a previous study, gradient PCR was used to optimize ideal annealing temperature ranges from 53-62°C and revealed that 58°C produced the best outcomes for the ToxR primer with a size of 171 base pairs. Real-Time PCR was utilized to amplify, specify, and test for sensitivity under the ideal conditions from the PCR Gradient. The confirmation results show that the primer pairs could amplify ToxR of Vibrio parahaemolyticus with the amount of concentration as much as 50 ng/µL with Ct 10,69 and 10,32 and melting curve at temperature 82,18°C and 82,23°C. This primer pair can also distinguish non-target bacteria with different Ct and melting curve temperature. The sensitivity assay for this primer can amplify DNA templates at concentration 0,0032 ng/µL. Shrimp samples that are contaminated artificially can still be detected at Ct 13,02 and Ct 13,09. Based on these results, it can be concluded that Real Time PCR with ToxR primer can be applied to develop a detection kit for Vibrio parahaemolyticus in seafood.
Rapid Detection of Foodborne Pathogen Bacteria Vibrio parahaemolyticus in Seafood using Gene ToxR with Real-Time Polymerase Chain Reaction Method Ismaya Krisdawati; Muktiningsih Nurjayadi; Jefferson Lynford Decan
Microbiology Indonesia Vol. 17 No. 1 (2023): March
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5454/mi.17.1.15-23

Abstract

Cases of food poisoning often occur due to food contamination caused by pathogenic bacteria. One of the pathogenic bacteria is Vibrio parahaemolyticus which is found in seafood. Thus, a fast, accurate and specific detection method is needed. The purpose of this study was to quickly detect Vibrio parahaemolyticus bacteria in seafood samples targeting the ToxR gene using Real Time PCR. In a previous study, gradient PCR was used to optimize ideal annealing temperature ranges from 53-62°C and revealed that 58°C produced the best outcomes for the ToxR primer with a size of 171 base pairs. Real-Time PCR was utilized to amplify, specify, and test for sensitivity under the ideal conditions from the PCR Gradient. The confirmation results show that the primer pairs could amplify ToxR of Vibrio parahaemolyticus with the amount of concentration as much as 50 ng ?L-1 with Ct 10.69 and 10.32 and melting curve at temperature 82.18°C and 82.23°C. This primer pair can also distinguish non- target bacteria with different Ct and melting curve temperature. The sensitivity assay for this primer can amplify DNA templates at concentration 0.0032 ng ?L-1. Shrimp samples that are contaminated artificially can still be detected at Ct 13.02 and Ct 13.09. Based on these results, it can be concluded that Real Time PCR with ToxR primer can be applied to develop a detection kit for Vibrio parahaemolyticus in seafood.
Rapid detection of Vibrio alginolyticus in seafood using the flgL gene and real-time polymerase chain reaction Muktiningsih Nurjayadi; Gladys Indira Putri; Jefferson Lynford Declan; Ismaya Krisdawati; Dandy Akbar Juliansyah; Maharanianska Azzahra; Irvan Maulana; Irma Ratna Kartika; Fera Kurniadewi; Tiara Fahriza; Adinda Myra Amalia Putri; Ayu Berkahingrum; Atikah Nur Rahmawati; Rosita Gio Anggraeni; Dalia Sukmawati; Sri Rahayu; Vira Saamia; I Made Wiranatha; Bassam Abomoelak; Hesham Ali El-Enshasy
Acta Biochimica Indonesiana Vol. 7 No. 1 (2024): Acta Biochimica Indonesiana
Publisher : Indonesian Society for Biochemistry and Molecular Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32889/actabioina.159

Abstract

Background: Seafood is highly nutritious but poses health risks when contaminated with pathogenic bacteria like Vibrio alginolyticus, which causes food poisoning and can infect marine animals and humans. Objective: This research aimed to determine the sensitivity and specificity of real-time polymerase chain reaction (rt-PCR) using the flgL primer pair to detect V. alginolyticus bacteria in seafood. Methods: The rt-PCR method was used to detect V. alginolyticus quickly, specifically, and sensitively. The flgL primer pair was evaluated for amplicon length, Ct value, Tm value, and its ability to differentiate between target and non-target bacteria. In this research, the samples tested were red snapper and blood clams. Results: The flgL primer produced an amplicon length of 224 bp. At 50 ng concentration, it yielded a Ct value of approximately 11.00 and a Tm of approximately 83°C. The flgL primer successfully differentiated between target and non-target bacteria. In sensitivity tests, it detected V. alginolyticus at concentrations as low as 1.86 x 10-3 ng/µL. Detection in seafood samples was also successful. Conclusion: The rt-PCR assay using the flgL primer pair effectively detects Vibrio alginolyticus in seafood with high specificity, sensitivity, and rapidity. These findings support its use for rapid and accurate detection of pathogenic bacteria in seafood.
Desiminasi Pengembangan Media Pembelajaran Modul Elektronik Kimia Generasi ke-4 pada Guru Kimia di Wilayah Jakarta Timur 2 Nurjayadi, Muktiningsih; Irma Ratna Kartika; Irwan Saputra; Siti Fatimah; Ririn Gustini; Uswatul Nisa; Rizkahana Syehfia; Nida Nur Afifah; Jefferson Lynford Declan; Pardiana; Sintia Mardita
Bahasa Indonesia Vol 21 No 01 (2024): Sarwahita : Jurnal Pengabdian Kepada Masyarakat
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21009/sarwahita.211.5

Abstract

generation electronic module is one of the chemistry learning media that can facilitate a three-way interaction between students, teachers and learning resources in 5.0 society era. Various chemical materials can be presented in the form of electronic modules equipped with applications according to the characteristics of the material. However, these developments are often not followed by the development of teacher competence in the field. So that there is a gap between scientific developments in Higher Education and conditions in the field. In this community service program, dissemination of the development of the 4th generation chemistry e-module has been carried out for MGMP-2 (Musyawarah Guru Mata Pelajaran) teachers in the East Jakarta Region through the dissemination of research results conducted in the Chemistry Education Program of FMIPA UNJ, that carried out face to face, starting with the delivery of material, Workshop, interactive discussion and coaching online blended learning. During the process of delivering the material, the participants enthusiastically followed it step by step. At the end of the session an evaluation questionnaire was given, the input and suggestions obtained were very positive. So, it is hoped that this activity can continue to be carried out and be useful in disseminating science and technology, increasing teacher competence and adding development information in tertiary institutions according to field needs. Abstrak Perkembangan teknologi sangat mempengaruhi perkembangan media pembelajaran kimia saat ini. Elektronik modul generasi ke-4 merupakan salah satu media pembelajaran kimia yang dapat memfasilitasi interaksi tiga arah antara siswa, guru dan sumber belajar di era society 5.0. Berbagai materi kimia dapat disajikan dalam bentuk modul elektronik yang dilengkapi dengan aplikasi sesuai karakteristik materinya. Namun perkembangan media pembelajaran tersebut sering tidak diikuti dengan perkembangan kompetensi guru di lapangan. Sehingga terjadi kesenjangan antara perkembangan keilmuan di Perguruan Tinggi dengan kondisi dilapangan. Pada program pengabdian pada masyakarat ini, telah dilakukan desiminasi pengembangan e-modul kimia generasi ke-4 bagi guru MGMP-2 (Musyawarah Guru Mata Pelajaran) Wilayah Jakarta Timur melalui desiminiasi hasil penelitian yang dilakukan di rumpun Kimia FMIPA UNJ yang dilakukan seacara tatap muka, dimulai dengan penyampaian materi, Workshop, diskusi interaktif dan pembinaan secara blanded learning. Selama proses penyampaian materi, peserta antusias mengikuti tahap demi tahap. Pada akhir sesi diberikan angket evaluasi, masukan dan saran yang didapat sangat positif. Sehingga diharapkan kegiatan ini dapat terus dilakuakan dan bermanfaat dalam menyebarluaskan IPTEK, meningkatkan kompetensi guru serta menambah informasi pengembangan di Perguruan Tinggi sesuai dengan kebutuhan dilapangan.
Development of Bacillus subtilis Detection Method Targeting Genes codY, narH, and ureC Using Polymerase Chain Reaction Nurjayadi, Muktiningsih; Berkahingrum, Ayu; Putri, Adinda Myra Amalia; Rahmawati, Atikah Nur; Anggraeni, Rosita Gio; Fahriza, Tiara; Declan, Jefferson Lynford; Putri, Gladys Indira; Krisdawati, Ismaya; Juliansyah, Dandy Akbar; Azzahra, Maharanianska; Maulana, Irvan; Kartika, Irma Ratna; Kurniadewi, Fera; Sukmawati, Dalia; Rahayu, Sri; Saamia, Vira; Wiranatha, I Made; Abomoelak, Bassam; El Enshasy, Hesham
JSMARTech: Journal of Smart Bioprospecting and Technology Vol. 5 No. 1 (2024): JSMARTech Volume 5, No. 1, 2024
Publisher : JSMARTech

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jsmartech.2024.005.01.26

Abstract

Bacillus subtilis, a causative agent of foodborne illness in bread, cakes, cereals, and cheese leading to symptoms like diarrhea and nausea, was investigated for its role in nosocomial infections, including endocarditis, bacteremia, septicemia, and meningitis. The research focused on three genes: codY, narH, and ureC, which respectively contribute to bacterial survival through biofilm and spore formation, acid resistance, and adaptation to anaerobic conditions. The objective of this study was to evaluate the codY, narH, and ureC genes using the Gradient PCR method for Bacillus subtilis detection in bread and cheese through Real-Time PCR. During the research, bacterial growth was observed with an OD600 of 1.438 in Tryptic Soy Broth (TSB), and colonies of medium size, smooth, cream-coloured, and round shape were successfully isolated on Tryptic Soy Agar (TSA). The DNA template used had a 71.5 ng/µL concentration with an A260/280 ratio of approximately 1.830. The annealing temperature for Gradient PCR used was 53-62°C. The primers successfully amplified codY (175 bp), narH (222 bp), and ureC (153 bp) gene amplicons. The optimal annealing temperature for the primers used was 60°C, as indicated by the presence of a single bright band in electrophoresis. Using these three different genes, testing can also be conducted with the Multiplex PCR method. The next step involves developing a detection kit using optimized primers and annealing temperatures for the identification of Bacillus subtilis in bread and cheese samples using Real-Time PCR.
The pipB Gene as Target for Development of Detection Method of Pathogenic Bacteria Salmonella typhi Using Real-time Polymerase Chain Reaction Nurjayadi, Muktiningsih; Gusti Angieta Putri; Ananda Indah Putri Sihombing; Puan Aqila Azizah; Anisa Fitriyanti; Royna Rahma Musie; Helzi Angelina; Grace; Agus Setiawan; Dandy Akbar Juliansyah; Jefferson Lynford Declan; Gladys Indira Putri; Siti Fatimah; Adinda Myra Amalia Putri; Vira Saamia; Irma Ratna Kartika; Fera Kurniadewi; Shyi-Tien Chen; Bassam Abomoelak; Hesham A. El Enshasy
HAYATI Journal of Biosciences Vol. 32 No. 3 (2025): May 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.3.747-757

Abstract

Salmonella typhi is a bacteria that leads to typhoid fever and one of the causes of death due to bacteria infections. In Indonesia, typhoid fever occurs around 1,100 cases per 100,000 population per year, with a mortality rate of 3.1-10.4%. It's necessary to develop a rapid and accurate detection of Salmonella typhi. The pipB gene of Salmonella typhi has the function of being an autophagia inhibitor in humans. This study aims to develop a detection kit for Salmonella typhi pathogenic bacteria targeting the pipB gene using a pipB primer in confirmation, specificity, and sensitivity tests. The results showed that pipB primer can amplify Salmonella typhi DNA fragment of 196 bp at the optimum annealing temperatures between 54-62°C. Confirmation test with real-time PCR found that the pipB primer pair (pipB-F and pipB-R) amplified at cycle 12.93 and 13.10 (Duplo) with a Tm value of 84.05°C and 84.20°C (Duplo). Based on the difference and average value produced in the confirmation and specificity test, pipB primer has amplified Salmonella typhi DNA at Ct 12.47±0.6 with a Tm value of 83.62°C±0.6. The pipB primer pair (pipB-F and pipB-R) could distinguish target from non-target bacteria based on their cycle threshold (Ct) and melting temperature (Tm) values. The primer design of pipB primer pair (pipB-F and pipB-R) successfully detected Salmonella typhi bacteria with the smallest concentration of 55.78 × 102 CFU equivalent to 3.2 pg/µL. Based on the results, Salmonella typhi pipB primer successfully detected Salmonella typhi bacteria DNA rapidly, specifically, and sensitively using the real-time polymerase chain reaction method.
DISEMINASI PEMBELAJARAN STRUKTUR DAN FUNGSI BIOMOLEKUL PROTEIN BAGI GURU WILAYAH MGMP-1 JAKARTA TIMUR Nurjayadi, Muktiningsih; Saputra, Irwan; Putri, Gladys Indira; Declan, Jefferson Lynford; Krisdawati, Ismaya; Juliansyah, Dandy Akbar; Hairishah, Nazrisya
Bahasa Indonesia Vol 20 No 01 (2023): Sarwahita : Jurnal Pengabdian kepada Masyarakat
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21009/sarwahita.201.3

Abstract

Materi biokimia di SMA dipelajari pada kelas XII dengan salah satu pembahasan mengenai biomolekul protein. Pada pandemi COVID-19, terjadi perubahan sistem pembelajaran yang membutuhkan berbagai inovasi agar pembelajaran berlangsung efektif. Program pengabdian pada masyakarat ini, melakukan pengembangan pembelajaran struktur dan fungsi biomolekul protein bagi guru MGMP Wilayah Jakarta Timur-1 melalui desiminiasi elektonik modul Struktur dan Fungsi Protein hasil penelitian sebelumnya serta praktikum berbasis bahan rumahan dengan teknik blended learning. Pelaksanaan P2M yang dilakukan bersama Tim UNJ pada Guru-guru Kimia MGMP Jakarta Timur wilayah 1 berjalan dengan lancar. Peserta sangat antusias mengikuti materi yang disampaikan dan ikut berkontribusi dalam pelaksanaan dan mengamati praktikum uji kualitatif asam amino dan protein menggunakan bahan-bahan yang ada disekitar rumah, ramah lingkungan, dan sekaligus menerapkan konsep green chemistry. Berdasarkan hasil umpan balik, 90% peserta merasa puas dengan penyampaian materi yang disampaikan, memperoleh wawasan baru, relevan dengan yang diharapkan, dapat diimplementasikan di sekolah, dan menimbulkan ide baru untuk mendesain pembelajaran yang inovatif. Sehingga diharapkan kegiatan pengabdian kepada masyarakat yang dilakukan oleh dosen Universitas Negeri Jakarta bekerjasama dengan MGMP wilayah Jakarta Timur 1 sebagai wilayah binaan yang meliputi kecamatan Cakung, Duren Sawit, Jatinegara, Matraman, dan Pulogadung, dapat bermanfaat dalam menyebarluaskan IPTEKS dan meningkatkan kompetensi guru.
Detection of the Yersinia enterocolitica Bacteria Targeting the myfA and ystA Genes in Contaminated Vegetable Samples using Real-Time PCR to Develop Rapid Detection of Food Poisoning Bacteria Nurjayadi, Muktiningsih; Anggraeni, Rosita GIo; Putri, Gladys Indira; Declan, Jefferson Lynford; Juliansyah, Dandy Akbar; Fahriza, Tiara; Putri, Adinda Myra Amalia; Berkahingrum, Ayu; Rahmawati, Atikah Nur; Kartika, Irma Ratna; Kurniadewi, Fera; Sukmawati, Dalia; Rahayu, Sri; Saamia, Vira; Wiranatha, I Made; Abomoelak, Bassam; El-Enshasy, Hesham Ali
HAYATI Journal of Biosciences Vol. 32 No. 4 (2025): July 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.4.989-1002

Abstract

Yersinia enterocolitica is a pathogenic bacterium with the ability to survive and multiply in food in a low-temperature environment that can cause death in humans. In previous studies, the optimum annealing temperature of ymoA, ystA, and ail gene primers with amplicons of 185 bp, 123 bp, and 192 bp, respectively, was successfully found. This study aims to develop a pathogenic bacteria detection kit with confirmation, sensitivity, and specificity of myfA and ystA primers in detecting Yersinia enterocolitica bacteria quickly and accurately using the real-time Polymerase Chain Reaction method. The results showed that myfA and ystA primers have optimum annealing temperatures at 60°C with amplicon lengths of 181 bp and 123 bp, respectively. Primer myfA was able to amplify the target with real-time PCR at Ct 12.07±1 and Tm 81±1°C, while the ystA primer at Ct 12.38±1 and Tm 83±1°C. myfA and ystA primers were also able to distinguish target and non-target bacteria based on Ct or Tm. The designed primers successfully detected Yersinia enterocolitica bacteria with the smallest concentration of 0.000439 ng/µL equivalent to 7.024 × 102 CFU. The detection limit obtained is smaller than the contamination threshold set by the Food and Drug Administration (BPOM). Primer myfA and ystA Yersinia enterocolitica also successfully detected the target bacteria in cabbage and lettuce samples artificially. Based on these results, myfA and ystA primers successfully detected Yersinia enterocolitica in vegetable samples using real-time PCR quickly, sensitively, specifically, and accurately.
DISEMINASI MODUL ELEKTRONIK KIMIA GENERASI KE- 4 BERBASIS ETNOSAINS DAN ECO-STEAM PADA GURU KIMIA DI MGMP WILAYAH JAKARTA SELATAN 2 Muktiningsih Nurjayadi; Fera Kurniadewi; Irma Ratna Kartika; Siti Fatimah; Nasywa Fhelia Salta; Athiyah Layla; Mellyna Fitriani; Annisa Maharani; Sarah Adilisa Kartini; Nabilla Anisa Putrie; Jefferson Lynford Declan
Prosiding Seminar Nasional Pengabdian Kepada Masyarakat Vol. 5 No. 1 (2024): PROSIDING SEMINAR NASIONAL PENGABDIAN KEPADA MASYARAKAT - SNPPM2024
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Negeri Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Abstract The rapid advancement of technology has profoundly influenced the development of learning media, accessibility, and the overall quality of education. The 4th generation electronic chemistry module represents an innovative educational tool that integrates visual, audio, audio-visual, and interactive features, facilitating enhanced communicating among learning resources, students, and educators to achieve learning objectives. This Community Service Program (P2M) aimed to disseminate research findings related to the 4th generation electronic module, which incorporates elements of ethnoscience and eco-STEAM. The dissemination activities were conducted with chemistry teachers from the South Jakarta 2 MGMP through a series of informational sessions and workshops. During the informational sessions, the theoretical framework underpinning the module's development was presented, along with an overview of the necessary applications, developmental procedures, and the integration of features such as videos, quizzes, images, pop-ups, and other interactive media tailored to specific instructional models. In the workshop component, participants were guided through the process of creating electronic modules, culminating in the successful production of a standardized prototype. The results showed that approximately 94.7% to 98.9% of participants were satisfied with the training. They found the delivery of the training material to be relevant and as expected, applicable to chemistry learning in schools, and inspiring for new interactive teaching ideas. Participants also felt that the presenter had a strong understanding of the topic, used the allotted time effectively, communicated the material clearly, answered questions well, and ensured that the training ran smoothly. Based on the findings, it is concluded that the dissemination of the 4th generation electronic chemistry module was highly effective. Keywords: The 4th generation Chemistry Electronic Module, Ethnoscience, Eco-STEAM, MGMP Abstrak Perkembangan teknologi yang semakin pesat memberikan dampak signifikan pada pengembangan media, aksesibilitas dan kualitas pembelajaran. Modul elektronik kimia generasi ke-4 merupakan Modul elektronik yang dapat menampilkan secara komprehensif, baik visual, audio, audio-visual, dan aktivitas interaktif antara sumber belajar, peserta didik dan guru untuk mencapai tujuan pembelajaran. Program Pengabdian kepada Masyarakat (P2M) ini bertujuan melakukan diseminasi produk penelitian tentang modul elektronik generasi ke-4 yang diwarnai dengan Etnosains dan Eco-STEAM. Diseminasi dilakukan pada guru-guru di MGMP Kimia wilayah Jakarta Selatan 2 dengan metode penyampaian materi scara diskusi informasi, dan workshop. Pada sesi materi disampaikan teori yang mendasari pengembangan modul, berbagai aplikasi yang dibutuhkan, langkah pengembangan, penambahan fitur video, kuis, gambar, teknik pop up, dan media interaktif lainnya yang dibuat berdasarkan alur model pembelajaran tertentu. Pada tahap workshop peserta diberikan kesempatan dan dibimbing dalam pembuatan elektronik modul sampai menghasilkan prototype standar. Kepuasan dari peserta diukur melalui kuesioner dan tanya jawab, hasilnya menunjukkan bahwa 94,7-98,9% peserta puas dengan penyampaian materi pelatihan, materi yang disampaikan relevan dan sesuai dengan yang diharapkan, dapat diterapkan dalam pembelajaran kimia di sekolah, memunculkan ide-ide baru untuk melaksanakan pembelajaran interaktif di kelas, pemateri sangat memahami topik yang disampaikan, waktu penyampaian materi mencukupi, penyampaian materi sangat baik dan mudah dipahami, pemateri menjawab pertanyaan peserta dengan sangat baik dan pelatihan berlangsung dengan lancar. Berdasarkan hasil yang diperoleh disimpulkan bahwa diseminasi modul elektronik kimia generasi ke-4 telah berhasil dengan sangat baik. Kata Kunci: Modul Elektronik Kimia generasi ke-4, Etnosains, Eco-STEAM, MGMP
Deteksi Vibrio parahaemolyticus Menggunakan Primer Gen toxR2 dengan Gradient Polymerase Chain Reaction Nurjayadi, Muktiningsih; Krisdawati, Ismaya; Declan, Jefferson Lynford; Putri, Gladys Indira; Juliansyah, Dandy Akbar; Rahmawati, Atikah Nur; Fitriyanti, Anisa; Musie, Royna Rahma; Kurniadewi, Fera; Sukmawati, Dalia; Saamia, Vira; Wiranatha, I Made; Abomoelak, Bassam; Elenshasy, Hesham Ali
Jurnal Riset Sains dan Kimia Terapan Vol. 11 No. 1 (2025): Jurnal Riset Sains dan Kimia Terapan, Volume 11 Nomor 1, Juni 2025
Publisher : Program Studi Kimia Universitas Negeri Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21009/JRSKT.111.03

Abstract

Makanan adalah kebutuhan vital, dengan kriteria utama adalah keamanan, kualitas, dan nilai gizi. Untuk memastikan keamanan makanan, diperlukan metode deteksi yang cepat dan akurat, terutama untuk mendeteksi bakteri patogen penyebab keracunan makanan. Vibrio parahaemolyticus merupakan bakteri patogen yang banyak ditemukan pada makanan laut. Penelitian ini bertujuan untuk mengembangkan metode deteksi cepat V. parahaemolyticus dengan menargetkan gen toxR2 menggunakan Gradient Polymerase Chain Reaction. Gen toxR dipilih karena fungsinya sebagai pengatur penting gen virulensi. Tahapan yang dilakukan meliputi desain primer, penyiapan sampel bakteri dari biakan murni, dan uji amplifikasi menggunakan PCR Gradien. Hasil uji amplifikasi menunjukan bahwa pasangan primer toxR2 berhasil mengamplifikasi pada suhu 58-62°C dengan dihasilkan pita berukuran 137 bp. Berdasarkan hasil tersebut, dapat disimpulkan bahwa PCR Gradien dengan primer toxR2 dapat diaplikasikan untuk mengembangkan alat deteksi V. parahaemolyticus dengan dilakukan uji lanjutan seperti uji konfirmasi, uji spesifisitas, uji sensitivitas, dan uji pada pangan menggunakan Real-Time Polymerase Chain Reaction.
Co-Authors Aboemolak, Bassam Abomoelak, Bassam Abumoelak, Bassam Adinda Myra Amalia Putri Adinda Myra Amalia Putri Agus Setiawan AGUS SETIAWAN Akbar, Dandy Ananda Indah Putri Sihombing Angelina, Helzi Anggraeni, Rosita Gio Anisa Fitriyanti Annisa Maharani Athiyah Layla Atikah Nur Rahmawati Ayu Berkahingrum Azizah, Puan Aqila Azzahra, Maharanianska Bassam Abomoelak Bassam Abomoelak Berkahingrum, Ayu Chen, Shyi-Tien Dalia Sukmawati Dandy Akbar Juliansyah Dandy Akbar Juliansyah Dandy Akbar Juliansyah Dwi Ana Oktaviani El Enshasy, Hesham El-Enshasy, Hesham Ali Elenshasy, Hesham Ali Enshasy, Hesham Ali El Fahriza, Tiara Fera Kurniadewi Fera Kurniadewi Fitriyanti, Anisa Gladys Indira Putri Gladys Indira Putri Gladys Indira Putri Grace Grace, Grace Gusti Angieta Putri Hairishah, Nazrisya Helzi Angelina Hesham A. El Enshasy Hesham Ali Al-Enshashy Hesham Ali El-Enshasy I Made Wiranatha I Made Wiranatha Irma Ratna Kartika Irvan Maulana Irvan Maulana Irwan Saputra Ismaya Krisdawati Ismaya Krisdawati Ismaya Krisdawati Juliansyah, Dandy Akbar Kartika, Irma Ratna Krisdawati, Ismaya Kurniadewi, Fera Maharani Azka Azzahra Maharanianska Azzahra Maulana, Irvan Mellyna Fitriani Muktiningsih Nurjayadi Musie, Royna Rahma Nabilla Anisa Putrie Nasywa Fhelia Salta Nida Nur Afifah NOVITASARI Pardiana Puan Aqila Azizah Putri, Adinda Myra Amalia Putri, Gladys Indira Putri, Gusti Angieta Rahmawati, Atikah Nur Ririn Gustini Rizkahana Syehfia Rosita Gio Anggraeni Royna Rahma Musie Saamia, Vira Saamia4, Vira Saputro, Dwi Anna Oktaviani Sarah Adilisa Kartini Shangkara, Muhammad Arkent Shyi-Tien Chen Sihombing, Ananda Indah Putri Sintia Mardita SITI FATIMAH Siti Fatimah Sri Rahayu SRI RAHAYU Tiara Fahriza Uswatul Nisa Vira Saamia Vira Saamia Vira Saamia Wiranatha, I Made