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Iman Rusmana
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rusmana13@yahoo.com
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microbiology.indonesia@gmail.com
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kPERHIMPUNAN MIKROBIOLOGI INDONESIA (SeKretariat PERMI), Gedung 10.2 Indonesian Life Sciences Center (ILSC), Zona Bisnis Teknologi Puspiptek, Jalan Raya Serpong - Bogor Gunung Sindur, Jawa Barat 16340, Indonesia. Email: microbiology.indonesia@gmail.com
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Kota tangerang,
Banten
INDONESIA
Microbiology Indonesia
Published by Perhimpunan Mikrobiologi Indonesia
ISSN : 19783477     EISSN : 20878575     DOI : -
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology
Microbiology Indonesia provides a unique venue for publishing original researches in microbiology (espesially from Indonesian reseachers), and ensures that authors could reach the widest possible audience. Microbiology Indonesia publishes a wide range of research disciplines on bacteria, archaea, fungi, protozoa, and virus as well as biotechnology related to microbiology. Topics include (but are not limited to): -methods in microbiology, -bioprocess, -environmental microbiology, -food microbiology, -plant-microbe interaction, -animal-microbe interactions, -microbial community, -microbial genetics, -virology, -comparative and functional microbial genomics, -and gene expression in microbes.
Arjuna Subject : Imunologi dan Mikrobiologi (semua kategori) - Mikrobiologi dan Bioteknologi Terapan
Articles 398 Documents
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Medium Optimization for Penicillin Acylase (PAc) Production by Recombinant B. megaterium MS941 Containing pac Gene from B. thuringiensis BGSC BD1 Using Response Surface Methodology FENTRI PARAMITHA PUTRI; ASTUTIATI NURHASANAH; NIKNIK NURHAYATI; IS HELIANTI; KHASWAR SYAMSU
Microbiology Indonesia Vol. 9 No. 2 (2015): June 2015
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1794.751 KB) | DOI: 10.5454/mi.9.2.3

Abstract

Penicillin G acylase (PAc) hydrolyses of the amide bond of benzylpenicillin (Pen-G) releasing PAA and 6-APA, key intermediate in the production of various semisynthetic penicillins. In this study, we optimised the production medium of PAc by RSM using two variables (xylose as inducer and CaCl2 as divalent cations) to obtain the optimum PAc specific activity from Bacillus megaterium btpacBD1. For this purpose, combinations of five different xylose concentrations (0.13 – 0.87 %) and five different CaCl2 concentrations (0.64 – 4.36 mM) were analysed, in a total of 22 experiments. CCD used for the analysis showed that in shake flask cultivations, xylose and CaCl2 showed significant effects on PAc volumetric activity and the quadratic model was in good agreement with the experimental results (R2= 0.86 (p-value < 0.0001)). The maximum specific activity (130.669 ± 50.241 units mg protein-1) was reached when xylose and CaCl2 concentrations were 0.49% and 2.4 mM, respectively, and medium pH was around 7. Under such conditions, the activity of PAc and protein concentration achieved were 1.318 ± 0.406 units mL-1 and  0.0101 ± 0.01 mg mL-1. The shake flask validation experiments demonstrated that with such medium composition the volumetric activity, protein concentration and specific activity achieved were 1.294 ± 0.171 units mL-1, 0.0102 ± 0.0003 mg mL-1 and 125.91 ± 13.309 units mg-1, respectively. When the optimum medium composition was applied in 10 L bioreactor, the optimum volumetric activity (2.0687 ± 0.0820 units mL-1) and protein concentration (0.0078 ± 0.0008 mg mL-1) were achieved 48 h after the start of the cultivation. However, the optimum PAc specific acivity (1260.52  ± 27.5711 units mg protein-1) was achieved 18 h after the start of the cultivation.
Antimicrobial Activity of Lactic Acid Bacteria from Bamboo Shoot Pickles Fermented at 15 oC LAKSMI HARTAYANIE; LINDAYANI LINDAYANI; MONIKA PALUPI MURNIATI
Microbiology Indonesia Vol. 10 No. 2 (2016): June 2016
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (268.625 KB) | DOI: 10.5454/mi.10.2.5

Abstract

Lactic Acid Bacteria (LAB) produces natural antimicrobial compounds that can inhibit and prevent the growth of spoilage bacteria. LAB can be isolated from fermented food such as pickles which fermented at  cool temperature. The objective of this research to isolate and to obtain  LAB from yellow bamboo (Dendrocalamus asper) shoots pickles that has antimicrobial activity against Escherichia coli  and Staphylococcus aureus.  It was made by submerged yellow bamboo shoots in 2.5%   of brine solution and kept into sealed container then fermented at chiller (15oC) temperatures for 10 days. LAB was isolated using MRS agar and identified base on their morphological, physiological and biochemical characteristics. The result showed that LAB isolates identified as Lactobacilli and had antimicrobial activity against Escherichia coli  and Staphylococcus aureus.  All Lactobacilli (21 isolates) that was isolated from fermentation at 15oC were homofementative.
Cloning and Heterologous Expression of Extracellular Plantaricin F Produced by Lactobacillus plantarum S34 Isolated from “Bekasam” in Lactococcus lactis
Microbiology Indonesia Vol. 10 No. 3 (2016): September 2016
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2231.264 KB) | DOI: 10.5454/mi.10.3.3

Abstract

Plantaricin F (pln F) is bacteriocins produced by Lactobacillus plantarum are mostly applied in food to prevent microbial contamination. Biosynthesis of pln F is controlled by plantaricin A (pln A) which is primarily a peptide pheromone that controls the production of antimicrobial peptides in L. plantarum. Pre-mature pln A contains signal peptide and utilizes the general secretory pathway for export this peptide. The aim of this study was to construct a fusion of pln A signal peptide with mature pln F and to investigate the antimicrobial activity of pln F. Extracellular pln A- encoding the plnA gene were cloned into pGEM-Teasy vector to be used as a source for signal peptide SPplnA. A polymerase chain reaction (PCR) overlaps technique has been used in the construction of the fused gene with size of 171 bp while the individual gene obtained by this technique was 66 bp for pln A signal peptide and 105 bp for pln F. A gene encoding the pln A signal peptide (SPplnA) fused to mature plantaricin F,  fused gene were then cloned into pNZ8148 as expression vector under the control of the nisin promoter (Pnis A) to generate a pNZ8148 SPplnA-plnF. Molecular expression study showed that recombinant Lactococcus lactis NZ3900 was able to express the mature pln F at transcription and translation level with size of 171 bp (by RT-PCR) and 3.8 kDa (by SDS-PAGE), respectively after 0.5-5 ng/ml nisin induction (OD600 0,5). Furthermore, the supernatants of the recombinant L. lactis NZ3900 showed antimicrobial activity against Escherichia coli ATCC 8739, Staphylococcus aureus ATCC 6539 and Listeria monocytogenes BTCC B693. Collectively, the successfulness of expression of functional pln F gene under the control of nisin induction in L. lactis NZ3900, for the first time.
High Prevalence of Occult Hepatitis B Infection (OBI) and its Molecular Characteristics among Pregnant Women in Surabaya, Indonesia
Microbiology Indonesia Vol. 10 No. 1 (2016): March 2016
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2772.175 KB) | DOI: 10.5454/mi.10.1.1

Abstract

Perinatal transmission is the predominant mode of hepatitis B virus (HBV) transmission in countries where HBV infection is endemic. Newborns of HBV infected mothers have a high risk (up to 90%) of chronicity through perinatal transmission. HBsAg serology screening has been recommended to pregnant women, to prevent perinatal HBV infection. However, at present HBV DNA can be detected in serum with negative HBsAg (OBI - occult hepatitis B infection). The aim of this study was to determine the prevalence of occult hepatitis B infection (OBI) in pregnant women in Surabaya, Indonesia and its virological characteristics. A total of 50 HBsAg-negative and anti-HBc-positive sera were tested for anti-HBs and HBeAg. HBV DNA was isolated from these samples, analyzed by polymerase chain reaction (PCR) and sequenced. HBV DNA was detected in 9 (18%) samples, based on part of the S gene sequence. HBV/B3-adw2 was found predominant in 7 (77.7%) samples, HBV/B9-ayw1 in 1 (11.1%) sample, and HBV/C7-adrq+ in 1 (11.1%) sample. Three samples had mutations (Q129H, T131N, M133S, T140I, T126I) in the ‘a’ determinant region, which may play a role in the undetectability of the virus by the common HBsAg detection kit. The prevalence of OBI in pregnant women from Surabaya is high, but still in line with the general population in Asia. Application of anti-HBc antibody or HBV DNA detection in screening would be very beneficial and prevent perinatal transmission from OBI pregnant women.
Cover and Table of Content
Microbiology Indonesia Vol. 9 No. 2 (2015): June 2015
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (202.662 KB) | DOI: 10.5454/mi.9.2.%p

Abstract

Editorial Board and Publisher Information
Microbiology Indonesia Vol. 9 No. 2 (2015): June 2015
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (120.654 KB) | DOI: 10.5454/mi.9.2.%p

Abstract

Guide for Authors Iman Rusmana
Microbiology Indonesia Vol. 9 No. 2 (2015): June 2015
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (331.053 KB) | DOI: 10.5454/mi.9.2.%p

Abstract

Membership Application Form of PERMI
Microbiology Indonesia Vol. 9 No. 2 (2015): June 2015
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (227.005 KB) | DOI: 10.5454/mi.9.2.%p

Abstract

Subscription Form of Microbiology Indonesia Iman Rusmana
Microbiology Indonesia Vol. 9 No. 2 (2015): June 2015
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (81.35 KB) | DOI: 10.5454/mi.9.2.%p

Abstract

Back Cover and Sponsor Information Iman Rusmana
Microbiology Indonesia Vol. 9 No. 2 (2015): June 2015
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (644.97 KB) | DOI: 10.5454/mi.9.2.%p

Abstract

Page 11 of 40 | Total Record : 398

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