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Microbiology Indonesia
Published by Perhimpunan Mikrobiologi Indonesia
ISSN : 19783477     EISSN : 20878575     DOI : -
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology
Microbiology Indonesia provides a unique venue for publishing original researches in microbiology (espesially from Indonesian reseachers), and ensures that authors could reach the widest possible audience. Microbiology Indonesia publishes a wide range of research disciplines on bacteria, archaea, fungi, protozoa, and virus as well as biotechnology related to microbiology. Topics include (but are not limited to): -methods in microbiology, -bioprocess, -environmental microbiology, -food microbiology, -plant-microbe interaction, -animal-microbe interactions, -microbial community, -microbial genetics, -virology, -comparative and functional microbial genomics, -and gene expression in microbes.
Arjuna Subject : Imunologi dan Mikrobiologi (semua kategori) - Mikrobiologi dan Bioteknologi Terapan
Articles 398 Documents
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Characterization of chaperone-like activity of small heat shock protein (sHSP) isolated from Indonesian Traditional Food (Tempoyak ) Lactobacillus plantarum U10
Microbiology Indonesia Vol. 8 No. 4 (2014): December 2014
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (759.205 KB) | DOI: 10.5454/mi.8.4.7

Abstract

The characterization of small heat shock protein (sHSP) from tempoyak-originated Lactobacillus plantarum was investigated. The heat adaptive response proteins were ranging from 18 kDa to 51 kDa. Interestingly, the Intercellular Protein (IP) fraction of heat shocked-L.plantarum U10 exhibited chaperone like activity by the ability to prevent loss of proteinase K activity from denaturation. Furthermore, The sHSP gene that related to the predicted sHSP ±18 kDa protein were successfully identified by PCR method and this gene has 423 bp size. The sHSP gene has 140 amino acids (with unique motive at C-terminus T-L-P-K amino acid sequence) and has closely 100% identity with those L.plantarum isolated from food or non-food environment. Moreover, the gene encoding sHSP ±18 kDa protein was indeed up-regulated after L.plantarum U10 treated by heat shocking as proven by Reverse Transcriptase-PCR. This result suggested that sHSP ±18 kDa in our study may confers a survival advantage on Lactobacillus plantarum and capable of protecting the cell against under temperature stress.
Characterization and Pathogenicity of Fusarium oxysporum as the Causal Agent of Fusarium Wilt in Chili (Capsicum annuum L.) REJEKI SITI FERNIAH; BUDI SETIADI DARYONO; RINA SRI KASIAMDARI; ACHMADI PRIYATMOJO
Microbiology Indonesia Vol. 8 No. 3 (2014): September 2014
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (258.32 KB) | DOI: 10.5454/mi.8.3.5

Abstract

Fusarium wilt is a serious disease attacking chili plants in Central Java which cause lost of chili productivity. Fusarium wilt is caused by pathogenic fungi Fusarium oxysporum, which is host specific. The objectives of this research were to characterize the pathogenic F. oxysporum as the causal agent of fusarium wilt in chili plants and to observe the virulence of the pathogen. Fungal pathogen was isolated from Tawangmangu as an endemic area of fusarium wilt in Central Java. The fungi was characterized morphologically and identified molecularly by its internal transcribed spacer regions (ITS regions). Pathogenicity test was done to observe the virulence of the pathogen. One pathogenic strain was isolated from Tawangmangu, Karanganyar and was identified  morphologically and molecularly as F. oxysporum.  
Optimization of β-Cyclodextrin Production from Sago Starch using Recombinant Cyclodextrin Glycocyltransferase from Bacillus sp. A2-5a
Microbiology Indonesia Vol. 8 No. 3 (2014): September 2014
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5454/mi.8.3.6

Abstract

Cyclodextrin (CD) is a cyclic oligosaccharide molecule which is classified into three types: α-CD, β-CD, and γ-CD, each consists of 6, 7, and 8 glucose units. CDs are produced enzymatically using starch as the substrate catalyzed by CD glycosyltransferase (CGTase). This research was aimed to determine optimum condition of β-CD production from sago starch using recombinant CGTase (rCGTase) from Bacillus sp. A2-5a, and using the optimum condition, β-CD production was done at 100 mL scale using complexing agents. In this study, rCGTase was overproduced in Escherichia coli BL21(DE3) and purified applying Ni-NTA affinity chromatography with gradual elution of imidazole and concentrated using Nanosep column. The purified rCGTase was 76.39 kDa in size and displayed β-cyclization and hydrolysis activities using zymography. The results showed that optimum conditions for β-CD production was achieved when preheated sago starch of 0.5% (w/v), enzyme of 2.6x10-2 U, and N,N-dimethylformamide (DMF) as complexing agent of 1% (v/v) were used and the reaction time was 8 h. When DMF of 1% (v/v) was added repeatedly at 100 mL scale production, the highest concentration of β-CD was obtained at the reaction time of 8 h. This research reported for the first time the optimum condition of b-CD production at 100 mL scale using rCGTase from Bacillus sp. A2-5a with DMF as complexing agent.
Isolation and Molecular Identification of Endophytic Bacteria from Durian Arillus (Durio zibethinus Murr.) var. Matahari
Microbiology Indonesia Vol. 8 No. 4 (2014): December 2014
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (922.724 KB) | DOI: 10.5454/mi.8.4.3

Abstract

Endophytes are plant-associated microorganisms that able to form colonies in internal tissue and considered as an important component of biodiversity. However, information about the existence of naturally fruits-associated endophytic bacteria at different life-history stages of hosts is very limited. Durian (Durio zibethinus Murr.) is an exotic tropical fruits with a high economical value, but the occurrence and functional role of associated endophytes remains unexplored. A total of sixteen endophytic bacterial isolates were identified by 16S rRNA sequence analysis from ripe and unripe stages of Durian fruits var. Matahari. These isolates belonged to the genus Staphylococcus, Bacillus, Enterobacter, Moraxella, Gordonia, Salmonella, Rhizobium, Brachybacterium, Kocuria, and Klebsiella. This is the first report of an endophytic bacterial spesies residing in Durian arillus. This research also indicated potency of culturable endophytes from Durian fruits in plant growth promotion.
Modified Slide Culture Method for Faster and Easier Identification of Dermatophytes YEVA ROSANA; TETSUHIRO MATSUZAWA; TOHRU GONOI; ANIS KARUNIAWATI
Microbiology Indonesia Vol. 8 No. 3 (2014): September 2014
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1069.613 KB) | DOI: 10.5454/mi.8.3.7

Abstract

Basic slide culture as a morphological identification was known as the most common method for the identification of pathogenic mold fungi. This method preserved the morphological features relatively undisturbed compared with adhesive tape preparations. However, it was necessary to modify method of basic slide culture to improve its usability and shorten the time it needed to identify mold fungi. There were four kinds of method carried out in this study; two kinds of modified slide culture, one kind of direct culture on slant agar plate, and a basic slide culture for identifying mold fungi, which result would be compared with each other. These four methods were tested to 4 species of dermatophytes which were known as mold fungi that could infect skin, hair, and nails in human; those were Trichophyton mentagrophytes, Microsporum canis, Microsporum gypseum, and Epidermophyton floccosum. Result of this study showed that both modified slide culture and direct culture on slant agar plate could visualize the structure of dermatophytes faster than basic slide culture method. These methods were also easier to prepare compared to basic culture method. Conclusion of this study showed that basic slide culture method needed to be modified for better identification of mold fungi.
Cloning, Sequencing, and Expression of the Gene Encoding a Family 9 Cellulase from Bacillus licheniformis F11 in Escherichia coli and Bacillus megaterium, and Characterization of the Recombinant Enzymes IS HELIANTI; MARIA ULFAH; NIKNIK NURHAYATI; LLINA MULYAWATI
Microbiology Indonesia Vol. 8 No. 4 (2014): December 2014
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (913.463 KB) | DOI: 10.5454/mi.8.4.2

Abstract

 A gene encoding cellulase belonging to the glycosyl hydrolase family 9 along with its native promoter was isolated from Bacillus licheniformis F11, cloned in Escherichia coli DH5 α and subcloned by transconjugation to Bacillus megaterium MS941. Functionality of the encoded protein was proven both in heterologous hosts, E. coli and B. megaterium. In the latter, the gene product was found in the extracellular fraction expressing a high specific activity; whereas in E. coli the protein was not secreted into the medium, and rather, showed a lower specific activity. The optimum temperature of the recombinant enzyme expressed in the hosts range from 65-75 ºC; whereas the optimum pH is 6. The recombinant enzyme was stable between 50-60 ºC and in a broad pH range (pH 5 - 9). Addition of Ca2+ and Fe3+ enhanced the enzyme activity, whereas EDTA and Cu2+  had the opposite effect. Lichenin, rather than carboxyl methyl cellulose, is the preferred substrate.
Effect of Lactic Acid Filtrate and Bacteriocins of Lactobacillus acidophillus on Phagocytosis Activity of Macrophages Cell againts Enteropathogen Escherichia coli (EPEC) IIS HERAWATI; DIKI HILMI; PRIMA NANDA FAUZIAH
Microbiology Indonesia Vol. 8 No. 4 (2014): December 2014
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1073.545 KB) | DOI: 10.5454/mi.8.4.6

Abstract

Immunity development known as one of effective ways in avoiding infection. Antibacterial agent product isolated from Lactobacillus acidophillus has been  reported can activate T lymphocyte as part of adaptive immunity. This experimental study aimed at investigation of lactic acid and bacteriocins filtrate from L. acidophillus in modulating phagocytosis activity of human macrophages infected by enteropathogenic Escherichia coli (EPEC). Each of human macrophages culture was supplemented with lactic acid and bacteriocins filtrate at concentration of 3.125, 6.25, and 12.5 µg mL-1 as well as control without filtrate addition and incubated for 24 h. Macrophages culture was then infected with EPEC for 30 minutes and was microscopically observed after being stained by Giemsa. Percentage of phagocytosis activity was gained from active macrophages in 100 observed cells. Macrophages cultures supplemented with bacteriocins filtrate showed augmented phagocytosis activity while cultures supplemented with lactic acid filtrate showed decreased phagocytosis activity. ANOVA analysis showed significant difference in phagocytosis activity of macrophage cultures supplemented with lactic acid (p=0,038) and bacteriocins (p=0,016 and 0,023). Tukey HSD analysis for phagocytosis activity of macrophage cultures supplemented by bacteriocins, each group of treatment showed significant difference againts control. In conclusion, lactic acid from  L.acidophillus has no effect in modulation of macrophages phagocytosis activity while bacteriocins can improve phagocytic activity. Bacteriocins from L. acidophillus then can be suggested to have a role as immunomodulator.
Codon Optimization and Chaperone Assisted Solubilization of Recombinant Human Prethrombin-2 Expressed in Escherichia coli SARONOM SILABAN; IMAN PERMANA MAKSUM; SHABARNI GHAFFAR; KHOMAINI HASAN; SUTARYA ENUS; TOTO SUBROTO; SOETIJOSO SOEMITRO
Microbiology Indonesia Vol. 8 No. 4 (2014): December 2014
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (692.802 KB) | DOI: 10.5454/mi.8.4.5

Abstract

Prethrombin-2 (PT2) is a thrombin precursor, which plays a role in the conversion of fibrinogen into fibrin during blood clotting process. Previous study reported that the expression of human prothrombin-2 (rhPT2) in Escherichia coli formed inclusion bodies. The aim of this study was to establish a strategy to express a soluble rhPT2 in E. coli. This study was animed to design and codon optimize human prethrombin-2 gene as well as to optimize the expression condition using four strains of E. coli. The codon adaptation index (CAI) of the unoptimized hpt2 gene was 0.336, with 56.8% GC content. After optimization, the CAI of optimized hpt2 became 1.000 with 53.1% GC content. The optimized gene was successfully cloned into pTWIN1 expression vector. Expression analysis indicated that only E. coli ArcticExpress strain could successfully express a soluble recombinant rhPT2 protein, with only part of rhPT2 being expressed in insoluble form. However, the rest of the E. coli strains used in the experiments failed to express the rhPT2 in soluble form. We are deducing that the success in achieving soluble expression was not only due to the availability of chaperonins Cpn60/Cpn10, which played a crucial role in the protein folding in E. coli ArcticExpress strain, but also due to the codon optimization of hpt2 gene.
Cloning and Expression of Endoglucanase Gene from Thermophilic Bacteria Bacillus sp. RP1 MAELITA RAMDANI MOEIS; DESSY NATALIA; RAHMA WIDYA NINGRUM; ARI DWIJAYANTI
Microbiology Indonesia Vol. 8 No. 4 (2014): December 2014
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (814.773 KB) | DOI: 10.5454/mi.8.4.4

Abstract

An endoglucanase gene from glycoside hydrolase family 5, had been isolated from Bacillus sp. RP1 and cloned into Escherichia coli. The cloned gene comprised the promoter, coding sequence and terminator of the gene.  This gene encoded a protein with 499 amino acid residues (Mr=55.2 kDa) with a typical Bacillus signal peptide. The recombinant endoglucanase (EG) had optimum activity at pH 5.0 and 50 °C. The recombinant EG was expressed in the extracellular, intracellular, and periplasmic fractions with the highest total activity (60.15%) in the intracellular fraction, measured at three hours after isopropyl-β-Dthiogalactopyranoside (IPTG) induction. Three hours after the addition of 1% carboxymethyl cellulose (CMC), there was a two-fold increase in intracellular EG specific activity compared to the uninduced cells. Three hours after the addition of 1 mM IPTG, 1% glucose, 1% galactose or 1% cellobiose the intracellular EG specific activity decreased compared to the uninduced cells.
Genetic Diversity of Cucumber Mosaic Virus Strain Soybean from Several Areas TRI ASMIRA DAMAYANTI; SURYO WIYONO
Microbiology Indonesia Vol. 9 No. 1 (2015): March 2015
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (872.412 KB) | DOI: 10.5454/mi.9.1.6

Abstract

Cucumber mosaic virus strain soybean (CMV-S) is one of economically important virus infecting soybean in Indonesia. However, it is very few information related with the CMV-S Indonesia isolates. Thus, the aim of present work is to detect and identify CMV-S isolates from different origin. Leaf samples collected from six different soybean fields in Java and North Sumatera. Molecular detection was carried out by reverse transcription polymerase chain reaction (RT-PCR) using specific primer of the coat protein (CP) gene and DNA sequencing. RT-PCR were successfully amplified the entire CP gene size ~657 bp from all samples and encoded 218 amino acids. Analysis of nucleotide and amino acid sequences showed high homology among isolates ranging from 99.5% and 100%. However isolate from Sindang Laut either nucleotides or amino acids showed lower homology to that of five isolates ranging from 89.4%-89.6% and 91.7%, respectively. The differences between CMV-S with other non legume strain found in six amino acid positions in the CP gene, suggesting the differences might related with host specificity. Phylogenetic analysis of amino acid of six isolates to other CMV strain showed that the five CMV-S isolates were in the same cluster, while Sindang Laut isolate was in different cluster together with the non-legume CMV from India. It is indicating the present of genetic variability among CMV-S isolates in Java.

Page 9 of 40 | Total Record : 398

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