cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
Iman Rusmana
Contact Email
rusmana13@yahoo.com
Phone
+62217560536
Journal Mail Official
microbiology.indonesia@gmail.com
Editorial Address
kPERHIMPUNAN MIKROBIOLOGI INDONESIA (SeKretariat PERMI), Gedung 10.2 Indonesian Life Sciences Center (ILSC), Zona Bisnis Teknologi Puspiptek, Jalan Raya Serpong - Bogor Gunung Sindur, Jawa Barat 16340, Indonesia. Email: microbiology.indonesia@gmail.com
Location
Kota tangerang,
Banten
INDONESIA
Microbiology Indonesia
Published by Perhimpunan Mikrobiologi Indonesia
ISSN : 19783477     EISSN : 20878575     DOI : -
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology
Microbiology Indonesia provides a unique venue for publishing original researches in microbiology (espesially from Indonesian reseachers), and ensures that authors could reach the widest possible audience. Microbiology Indonesia publishes a wide range of research disciplines on bacteria, archaea, fungi, protozoa, and virus as well as biotechnology related to microbiology. Topics include (but are not limited to): -methods in microbiology, -bioprocess, -environmental microbiology, -food microbiology, -plant-microbe interaction, -animal-microbe interactions, -microbial community, -microbial genetics, -virology, -comparative and functional microbial genomics, -and gene expression in microbes.
Arjuna Subject : Imunologi dan Mikrobiologi (semua kategori) - Mikrobiologi dan Bioteknologi Terapan
Articles 398 Documents
 8   9   10   11   12 
Molecular Phylogeny of Salmonellae: Relationships among Salmonella Species Determined from gyrA, gyrB, parC, and parE Genes CHARIS AMARANTINI; DHIRA SATWIKA
Microbiology Indonesia Vol. 9 No. 1 (2015): March 2015
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (397.474 KB) | DOI: 10.5454/mi.9.1.1

Abstract

Study on molecular characteristics of Salmonella from clinical isolates was done in order to find out its relationship, especially those isolated from Indonesia. Partial sequence of genes belonging to QRDR region, i.e. gyrA, gyrB, parC, and parE were employed. Specific primer pairs covering those genes are used to amplify the bacterial DNA obtained. The amplicons were then analyzed by means of sequencing, and the sequences are analysed bioinformatically to find out similarities and build phylogenetic trees. By comparing all of the phylogenetic tree from QRDR region, this study revealed gyrA as the most suitable gene for rapidly identify member of salmonellae as it gives better separation of samples being analysed. However, the use of parC is recommended as it gives a consistent and reliable value to separate member of Salmonella and other Enterobacter. Further studies are under way to include member of this group, like E. coli, and the use of full sequence of QRDR genes region to verify this report.
Cloning and Expression of HA1 Gene of H1N1 Influenza Virus 2009 Pandemic (H1n1pdm09) Indonesia Strain in the Pichia Pastoris Expression System for the Development of Influenza Vaccine ASRI SULFIANTI; ANDI YASMON; BUDIMAN BELA; FERA IBIRAHIM
Microbiology Indonesia Vol. 9 No. 2 (2015): June 2015
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1209.728 KB) | DOI: 10.5454/mi.9.2.7

Abstract

Among influenza viral proteins, hemagglutinin 1 (HA1) is the target for neutralizing antibodies which inhibit virus binding to receptor of target cells. This protein is widely developed as subunit recombinant vaccine. In this research, we expressed HA1 protein recombinant from DKI271/2011 Indonesian strain in Pichia pastoris. The identity of this protein was confirmed by western blotting using anti-His T ag and mouse specific antibody HA H1N1pdm09. The use of yeast P . pastoris as an alternative strategy to solve the problems which commonly found in influenza vaccine productions. Expression protein in E. coli has been known to have many problems, while mammalian and insect cells requires special skills and relatively high cost. The analysis of HA1 gene sequences showed no mutation in epitope region which recognized by T dan B cells. Further, this recombinant protein can be used as vaccine candidate in influenza vaccine development.Keywords: Hemagglutinin; Pichia pastoris; vaccine; Influenza Virus; H1N1pdm09.
Isolation and Identification of Bacteria from Raw Materials Contaminated by Rope-producing Bacteria ANASTASIA ASRI WIDYASARI; STELLA MAGDALENA; BIBIANA WIDIYATI LAY
Microbiology Indonesia Vol. 9 No. 3 (2015): September 2015
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (328.587 KB) | DOI: 10.5454/mi.9.3.3

Abstract

Ropey bread is a bacterial spoilage condition of bread. The spoilage involved in ropey bread is primarily due to Bacillus subtilis. Several studies have shown that rope spoilage can be controlled using antimicrobial agents such as acetic acid, lactic acid, and quaternary ammonium cations (QACs/quats). This research consisted of five main steps: isolation, identification, confirmation, and molecular characterization of bacteria from raw materials contaminated by rope-producing bacteria, and antimicrobial test against rope-producing bacteria. The confirmation test was done in order to determine the rope-producing ability of isolates suspected as Bacillus sp. There were two different treatments in this test. In the first treatment, the inoculums were mixed with bread dough. In the second treatment, each slice of loaf was placed into a petri dish, uniformly soaked with inoculums. The first treatment did not show rope spoilage for all of the loaves, however, 6 of 11 loaves in the second treatment developed rope. The largest inhibition halos for antimicrobial test were produced by quats. This means quats is the strongest antimicrobial agent against rope-producing bacteria. The molecular characterization showed that all of the suspected isolates had 98–99% similarity to Bacillus subtilis.
Biosorption of Lithium Using Biofilm Matrix of Natural Microbial Consortium ANDI KURNIAWAN; TATSUYA YAMAMOTO
Microbiology Indonesia Vol. 9 No. 3 (2015): September 2015
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (67.967 KB) | DOI: 10.5454/mi.9.3.2

Abstract

This study examined the biosorption of lithium using biofilm matrices of natural microbial consortiums collected from Lake Biwa, Japan. The characterization of the biofilm polymer as a suggested binding site of biofilm was also revealed in this study. The followings were observed as results of  this study: 1) biofilm has both negatively and positively charged sites; 2) lithium adsorption by biofilm matrix is a physicochemical process mainly promoted by the electrostatic interaction between the ion and the charged sites of biofilm polymers; 3) the adsorbing lithium ion promote the desorption of ions from biofilms through ion exchange mechanism; 4) biofilms components changed seasonally and seems to affect the ability of biofilm to adsorb ions. According the results of this study, natural biofilm may become a promising biosorbent in the biosorption of lithium ion.
Antibacterial Activity and Mode of Action of (+)-2,2'-Epicytoskyrin A ANDRIA AGUSTA; DEWI WULANSARI; YULIASRI JAMAL; ARIEF NURKANTO; PRAPTIWI PRAPTIWI; AHMAD FATHONI
Microbiology Indonesia Vol. 9 No. 1 (2015): March 2015
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2156.354 KB) | DOI: 10.5454/mi.9.1.5

Abstract

Antibacterial activity of (+)-2,2'-epicytoskyrin A, a main metabolite from culture of fungal endophyte Diaporthe sp. GNBP-10, was investigated against several strains of Gram-positive, Gram-negative bacteria, and one strain of Mycobacterium smegmatis. (+)-2,2'-Epicytoskyrin A exhibited prominent activity against Staphylococcus aureus BCC 1452 with minimum inhibitory concentration (MIC) value of 0.06 μg mL-1. The effect of (+)-2,2'-epicytoskyrin A treatment to S. aureus, resulted in alteration of bacterial cell membrane with an increase of cation efflux, while the cytoplasmic content was not leaked out and finally resulted in cell damage.
Isolation, Identification and Screening of Antimicrobial Properties of the Marine-Derived Endophytic Fungi from Marine Brown Seaweed CHANGI WONG; PETER PROKSCH; LEE TUNG TAN; SAMUEL LIHAN; AAZANI MUJAHID; MORITZ MÃœLLER
Microbiology Indonesia Vol. 9 No. 4 (2015): December 2015
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (553.03 KB) | DOI: 10.5454/mi.9.4.1

Abstract

Marine seaweeds are known to produce valuable medicinal compounds such as antioxidants and anticoagulants, and have been reported to display antimicrobial activity against gram positive and gram negative bacteria. Several studies have identified so-called endophytic fungi living inside their hosts as the source of active compounds. In this study marine brown seaweed, Padina sp., was studied with regards to their endophytic fungi to assess if they are the source of the reported antimicrobial activity. Twenty fungal isolates were isolated from Padina sp. collected off Talang-Talang Island, Sarawak, Malaysia. All isolates were screened for their antimicrobial properties and 11 out of 20 isolates displayed positive results. DNA was successfully extracted for five isolates and sequence analysis grouped all of them with other endophytic fungi. “Fungus 2” seems to be related to a so far uncultured endophytic fungus. “Fungus 19” showed the most promising antimicrobial properties and was chosen for further agar well assay and cytotoxicity testing. Its ethyl-acetate extract showed positive results in the agar well assay and also a cytotoxic effect on Artemia nauplii. The extract was screened using HPLC and showed a compound similar to a known anti-cancer compound, dihydromyricetin, which is also an anti-intoxicant, anti-inflammatory and anti-oxidative agent which may be responsible for the observed antimicrobial activity.
Genetic Profiles of Escherichia coli Isolated from Indonesian Tempeh Based on Enterobacterial Repetitive Intergenic Consensus- Polymerase Chain Reaction (ERIC-PCR) QURROTA A’YUN; ANTONIUS SUWANTO; TATI BARUS
Microbiology Indonesia Vol. 9 No. 2 (2015): June 2015
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1097.305 KB) | DOI: 10.5454/mi.9.2.2

Abstract

Tempeh is a famous Indonesian fermented food derived from soybeans inoculated with Rhizopus sp. Tempeh production varies depend on the producers and often conducted in an uncontrolled condition. This condition could lead to the growth of Escherichia coli which is known as bacterial indicators of environmental hygiene. Some strains of E. coli could induce diarrhea, acute gastroenteritis or gastrointestinal tract infections. The aim of this study was to compare genetic diversity of E. coli isolates from tempeh with medical isolates employing ERIC-PCR method. In this study, 63f and 1387r primers were used to amplify 16S rRNA genes, and ERIC 1R and ERIC 2 primers were used for ERIC-PCR analysis. Tempeh samples were obtained from four producers in Bogor. Thirty-three isolates of E. coli were successfully isolated from tempeh samples produced by only two producers, we could not obtain E. coli isolates from the other two producers. In addition, the same tempeh samples could carry different genotypes of E. coli. On the other hand, the same genotypes could be found in different tempeh samples. Based on phylogenetic tree analysis, E. coli from tempeh could be separated from medical isolates. We showed that E. coli isolates derived from tempeh were genetically different from those of medical or pathogenic isolates.
Expression and Purification of PhoR Sensor-Domain Histidine Kinase of Mycobacterium tuberculosis in Escherichia coli ERNAWATI ARIFIN GIRI-RACHMAN; FENRYCO PRATAMA; OKTIRA ROKA AJI; ARUM PATRIATI; IHSANAWATI IHSANAWATI; MAELITA RAMDANI MOEIS; EDY GIRI-RACHMAN PUTRA
Microbiology Indonesia Vol. 9 No. 2 (2015): June 2015
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1382.183 KB) | DOI: 10.5454/mi.9.2.1

Abstract

Globally, tuberculosis (TB) remains a leading cause of death. The emergence of multidrug-resistant strains (MDR-TB) and extensively drug-resistant strains (XDR-TB) has fuelled the discovery for novel drugs and drug targets for its successful and better treatment. One of the potential candidates for drug target is PhoR sensory protein histidine kinase, a part of the Two Component System (TCS) PhoP/PhoR in Mycobacterium tuberculosis (Mtb). This protein system was known for its role on regulating hundred of Mtb virulence factors, from genes for cell wall and lypid synthesis to genes for adaptation in human leukocyte and hypoxia response. Previous studies have successfully characterized, isolated, and cloned the putative sensory domain of PhoR protein gene into pRSET vector expression system. In this study, Escherichia coli was transformed with pRSET-SensPhoR and cultivated at 37oC under IPTG induction to express PhoR sensor-domain protein. Most of the proteins were overexpressed in the form of inclusion bodies.  Subsequent protein purification in Ni-NTA system under refolding condition on urea gradient was performed to isolate PhoR sensor-domain protein in soluble form. Arginine was supplemented in purified protein solution to prevent aggregation during long term storage.  While highly purified protein was acquired, small angle X-ray scattering (SAXS) analysis was conducted to obtain 3-dimensional (3D) protein structures in solution.    doi:10.5454/mi.9.2.1 
Chemical Constituen from an Endophytic Fungus Aspergillus sp (SbD5) Isolated from Sambiloto (Andrographis paniculata Nees) ELFITA ELFITA; MUNAWAR MUNAWAR; MUHARNI MUHARNI; IHSAN IVANTRI
Microbiology Indonesia Vol. 9 No. 2 (2015): June 2015
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1207.015 KB) | DOI: 10.5454/mi.9.2.6

Abstract

Medicinal plants and their endophytes are important resources for discovery of natural products. Endophytic fungi isolated from medicinal plants more likely exibit pharmaceutical potentials. In the present study, an endophytic fungus Aspergillus sp (SbD5) was isolated from leaves of sambiloto (Andrographis paniculata). The fungus isolate was cultivated in Potato Dextrose Broth (PDB) medium for 7 weeks in static, then extracted with ethyl acetate followed by Thin Layer Chromatography (TLC) test. The results displayed three major spots. Ethyl acetate extract was further separated by column chromatography and recrystallization to obtain three pure compounds. Their structures were determined on the basic of spectroscopic analysis. The compounds is one new benzochromen derivative, 1-(3,8-dihydroxy-4,6,6-trimethyl-6H- benzochromen-2-yloxy)propan-2-one (1), together with two known compounds 5-hydroxy-4-(hydroxymethyl)-2H-pyran-2-one (2) and (5-hydroxy-2-oxo-2H-pyran-4-yl)methyl acetate (3). The antibacterial activities of the compounds were tested using the disk diffusion method against Staphylococcus aureus (ATCC 25923), Escherichia coli (ATCC 25922), Shigella dysenteriae and Salmonella typhi.  Compound 1 has the highest antibacterial activity followed by compound 2 and 3.
Inhibitory activity of Lactobacillus plantarum U10 isolated from Tempoyak (fermented durian) Made in Indonesia against Salmonella typhi SOGANDI SOGANDI; APON ZAENAL MUSTOPA; I MADE ARTIKA; BUGI RATNO BUDIARTO
Microbiology Indonesia Vol. 9 No. 2 (2015): June 2015
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1138.504 KB) | DOI: 10.5454/mi.9.2.5

Abstract

Lactobacillus plantarum U10 produced bacteriocin U10 which was isolated from a traditionally fermented food “tempoyak” from Sumatera Island in Indonesia. Production of the bacteriocins started at early exponential phase and reached maximum level at early stationary phase. Furthermore, plantaricins U10 was purified by ammonium sulphate precipitation followed by gel filtration chromatography. L. plantarum U10 produced two bacteriocins with a molecular mass of approximately 4.5 and 9.8 kDa by SDS-PAGE.  The mode of action of plantaricins U10 was identified as bactericidal agents against Salmonella typhi ATCC25241 as proven by CFU counting and SEM micrographs that showed differences in cell structures between treated cells and the non-treated control. SEM examination also confirmed structural destruction of membrane cells integrity and considerable morphological alteration of S.typhi.

Page 10 of 40 | Total Record : 398

 8   9   10   11   12 

Filter by Year

2007 2023


Reset
Filter By Issues
All Issue Vol. 17 No. 2 (2023): June Vol. 17 No. 1 (2023): March Vol. 16 No. 2 (2022): December Vol. 16 No. 1 (2022): March Vol. 15 No. 3 (2021): September 2021 Vol. 15 No. 2 (2021): June 2021 Vol. 15 No. 1 (2021): March 2021 Vol. 15 No. 4 (2021): December Vol. 14 No. 4 (2020): December 2020 Vol. 14 No. 3 (2020): September 2020 Vol. 14 No. 2 (2020): June 2020 Vol. 14 No. 1 (2020): March 2020 Vol. 13 No. 4 (2019): December 2019 Vol. 13 No. 3 (2019): September 2019 Vol. 13 No. 2 (2019): June 2019 Vol. 13 No. 1 (2019): March 2019 Vol. 12 No. 3 (2018): September 2018 Vol. 12 No. 2 (2018): June 2018 Vol. 12 No. 1 (2018): March 2018 Vol. 11 No. 4 (2017): December 2017 Vol. 11 No. 3 (2017): September 2017 Vol. 11 No. 2 (2017): Juni 2017 Vol. 11 No. 1 (2017): March 2017 Vol. 10 No. 4 (2016): December 2016 Vol. 10 No. 3 (2016): September 2016 Vol. 10 No. 2 (2016): June 2016 Vol. 10 No. 1 (2016): March 2016 Vol. 9 No. 4 (2015): December 2015 Vol. 9 No. 3 (2015): September 2015 Vol. 9 No. 2 (2015): June 2015 Vol. 9 No. 1 (2015): March 2015 Vol. 8 No. 4 (2014): December 2014 Vol. 8 No. 3 (2014): September 2014 Vol. 8 No. 2 (2014): June 2014 Vol. 8 No. 1 (2014): March 2014 Vol. 7 No. 4 (2013): November 2013 Vol. 7 No. 3 (2013): September 2013 Vol. 7 No. 2 (2013): June 2013 Vol. 7 No. 1 (2013): March 2013 Vol. 6 No. 4 (2012): December 2012 Vol. 6 No. 3 (2012): September 2012 Vol. 6 No. 2 (2012): June 2012 Vol. 6 No. 1 (2012): March 2012 Vol. 5 No. 4 (2011): December 2011 Vol. 5 No. 3 (2011): September 2011 Vol. 5 No. 2 (2011): June 2011 Vol. 5 No. 1 (2011): March 2011 Vol. 4 No. 3 (2010): December 2010 Vol. 4 No. 2 (2010): August 2010 Vol. 4 No. 1 (2010): April 2010 Vol. 3 No. 3 (2009): December 2009 Vol. 3 No. 2 (2009): August 2009 Vol. 3 No. 1 (2009): April 2009 Vol. 2 No. 3 (2008): December 2008 Vol. 2 No. 2 (2008): August 2008 Vol. 2 No. 1 (2008): April 2008 Vol. 1 No. 3 (2007): December 2007 Vol. 1 No. 2 (2007): August 2007 Vol. 1 No. 1 (2007): April 2007 More Issue