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Iman Rusmana
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kPERHIMPUNAN MIKROBIOLOGI INDONESIA (SeKretariat PERMI), Gedung 10.2 Indonesian Life Sciences Center (ILSC), Zona Bisnis Teknologi Puspiptek, Jalan Raya Serpong - Bogor Gunung Sindur, Jawa Barat 16340, Indonesia. Email: microbiology.indonesia@gmail.com
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INDONESIA
Microbiology Indonesia
Published by Perhimpunan Mikrobiologi Indonesia
ISSN : 19783477     EISSN : 20878575     DOI : -
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology
Microbiology Indonesia provides a unique venue for publishing original researches in microbiology (espesially from Indonesian reseachers), and ensures that authors could reach the widest possible audience. Microbiology Indonesia publishes a wide range of research disciplines on bacteria, archaea, fungi, protozoa, and virus as well as biotechnology related to microbiology. Topics include (but are not limited to): -methods in microbiology, -bioprocess, -environmental microbiology, -food microbiology, -plant-microbe interaction, -animal-microbe interactions, -microbial community, -microbial genetics, -virology, -comparative and functional microbial genomics, -and gene expression in microbes.
Arjuna Subject : Imunologi dan Mikrobiologi (semua kategori) - Mikrobiologi dan Bioteknologi Terapan
Articles 398 Documents
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Heterologous Expression of α-Amylase from Saccharomycopsis fibuligera R64 and its Tyr401Trp Mutant in Pichia pastoris RIEZKI AMALIA; WANGSA TIRTA ISMAYA; FERNITA PUSPASARI; KHOMAINI HASAN; TOTO SUBROTO; DESSY NATALIA; SOETIJOSO SOEMITRO
Microbiology Indonesia Vol. 10 No. 1 (2016): March 2016
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1791.165 KB) | DOI: 10.5454/mi.10.1.4

Abstract

α-Amylase from Saccharomycopsis fibuligera R64 is a non-adsorbing raw-starch degrading enzyme, a unique characteristic. This character is difficult to explain in the absence of its three-dimensional structure. Here we discuss the expression of a-amylase from Saccharomycopsis fibuligera in Pichia pastoris and the effect of site directed mutagenesis on its activity. A model based on the structure of its homologs suggested mutation of codon of Tyr401 into that of a Trp residue. An activity study using whole cells P. pastoris showed similar substrate degradation rates by cells carrying either the native or mutant amylase encoding gene. However, the purified enzyme of the mutant strain showed faster starch hydrolysis.
Cloning, Expression, and Functional Characterization of Autoactivated Human Prethrombin-2 Synthetic Gene by Using Pichia pastoris SMD1168 As a Host TOTO SUBROTO; WULAN PERTIWI; MUHAMMAD FADHILLAH; KHOMAINI HASAN; OGI BUDIANTORO; SUTARYA ENUS; SOETIJOSO SOEMITRO
Microbiology Indonesia Vol. 10 No. 2 (2016): June 2016
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4368.912 KB) | DOI: 10.5454/mi.10.2.1

Abstract

Prethrombin-2 is a thrombin precursor that has important role in blood coagulation. It is the smallest precursor which is activated into thrombin by FXa prior to coagulation process. However, as a commercial theurapetic protein in fibrin sealant component, prethrombin-2 must be activated by ecarin before used. Thus, the production process of this protein needs further purification. In order to eliminate ecarin activation step and to increase production efficiency, we designed, cloned and expressed the recombinant autoactivated human prethrombin-2 in Pichia pastoris SMD1168. The variant was designed with 4 mutations, E40A, D47A, G48P, and E52A, following the result of a previous study. The synthetic variant gene was first optimized to conform with P. pastoris codon preference. The optimized synthetic gene was cloned in pD912 plasmid using XhoI and SacII restriction enzymes. The transformed P. pastoris was selected on agar plate supplemented with 1,000 µg.mL-1 Zeocin as a selection marker. This study showed that autoactivated prethrombin-2 was succesfully expressed extracellularly by P. pastoris SMD1168. The activity of recombinant autoactivated prethrombin-2 using a chromogenic substrate S-2238 was 0.540 unit/mg. Taken together, these results demonstrated that autoactivated human prethrombin-2 was successfully produced extracellularly in P. pastoris.
Insecticidal Activities of Ethyl Acetate Extract of Indonesian Mangrove Fungus Emericella nidulans BPPTCC 6038 on Spodoptera litura SILVA ABRAHAM; ADI BASUKRIADI; SUYANTO PAWIROHARSONO; WELLYZAR SJAMSURIDZAL
Microbiology Indonesia Vol. 9 No. 3 (2015): September 2015
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (367.391 KB) | DOI: 10.5454/mi.9.3.1

Abstract

Mangrove fungi are known as sources of biological active compounds. The study and the report of secondary metabolites of mangrove fungi as insecticides is very limited in Indonesia. This study assess the insecticidal activities of ethyl acetate extract of Indonesian mangrove fungus Emericella nidulans BPPTCC 6038 against Spodoptera litura (Lepidoptera, Noctuidae) neonate larvae and pupae. The fungus E. nidulans BPPTCC 6038 was isolated from leaves of mangrove Rhizophora mucronata and identified based on ITS rDNA sequence data, with the GenBank accession number KP165435, and confirmed with morphological observation. This fungus strain was grown on malt extract broth for 14 days on rotary shaker at 65 rpm, and incubated at room temperature. Mortalities of S. litura were observed on larvae fed on artificial diet containing ethyl acetate extract of E. nidulans at concentrations of 625 – 5000 ppm. The lethal concentration of the extract which causes 50% mortality of larvae (LC50 value) was 1102.27 ppm. The other effects of fungus extract on S. litura were decrease in growth rate, longer larval period, inhibition on pupal development and absence in adult emergence. The HPLC analysis of extract showed that the crude extract contained three major compounds. This study provides evidence that the extract of E. nidulans possesses insecticidal activities against S. litura.
Antibiotic Use Is Not a Risk Factor of Infection by Extended-Spectrum Beta-Lactamase Producing Bacteria in Dr. Soetomo Hospital Surabaya
Microbiology Indonesia Vol. 9 No. 4 (2015): December 2015
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (483.637 KB) | DOI: 10.5454/mi.9.4.2

Abstract

Infection by Extended-Spectrum Beta-Lactamase (ESBL) producing bacteria confers a major challenge for clinicians due to limited treatment options and poor prognosis. Inappropriate antibiotic use is thought to cause the emergence of this resistant strain through selective pressure mechanisms. This study aims to describe the proportion of ESBL-producing bacteria and characteristics of patients with ESBL-producing bacterial infection, and to analyze the risk factors of infection by ESBL-producing bacteria in Dr. Soetomo Hospital, Surabaya. A cross-sectional study was conducted on medical records of inpatients of Internal Medicine Ward of Dr. Soetomo Hospital. Samples were classified into ESBL-positive or ESBL-negative groups. Demographic data, clinical data and previous antibiotic use of 66 samples (33 in each group) were retrospectively obtained. As many as 30 patients (45.5%) were male. Mean age of patients in the ESBL-positive and negative group were 53.57 (±16.77) and 54.27 (±14.88) years, respectively (p>0.05). The median pre-infection length of stay was 4 and 3 days for ESBL-positive and negative group, respectively (p>0.05). Type 2 diabetes mellitus was the most common comorbid disease (33.3%). The most frequent bacteria obtained from clinical isolates was Escherichia coli (49.3%). Proportion of ESBL producers amongst E.coli and K. pneumoniae isolates were 75% and 38.5%, respectively. The most frequently prescribed empirical antibiotic was ceftriaxone. None of the antibiotic used were risk factors for infection by ESBL-producing bacteria. Although none of the assessed variables were risk factors for ESBL-infection was discovered, this study finds a significantly larger proportion of ESBL-E. coli compared to non-ESBL producing E. coli. Further studies should include larger sample size and quantitatively measured antibiotic use.
Genotype of Hepatitis B Virus Coinfection in Typhoid Patients SUPIANA DIAN NURTJAHYANI; RETNO HANDAJANI
Microbiology Indonesia Vol. 9 No. 3 (2015): September 2015
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (79.254 KB) | DOI: 10.5454/mi.9.3.6

Abstract

Typhoid fever can cause liver disorder and may result in complication. Studies revealed hepatic involvement in thypoid known as typhoid hepatitis. Our previous paper reported the existance of hepatitis B virus (HBV) coinfection in the serum of patients with typhoid using nested-polymerase chain reaction (PCR). Based on sequence divergence, HBV has been classified into 10 genotypes (A-J), which in prediction response and correlates with clinical outcome of chronic HBV infection. This study was conducted to determine the genotypes of HBV in typhoid patients coinfected with HBV in Tuban. Sera obtained from 5 typhoid patients positive HBV by nested PCR). Study was performed by direct sequencing BigDye V1.1 Terminator Cycle Sequencing kit and ABI Prism 310 Genetic Analyzer. Analys had been using the Genetix version 10 software to create the phylogenetic tree. Phylogenetic analysis showed 3 samples as genotype B and 2 two samples as genotype C.
Optimization of Surfactin Production by Bacillus amyloliquefaciens MD4-12 using Response Surface Methodology AHMAD WIBISANA; WAHONO SUMARYONO; MIRAWATI SUDIRO; PRATIWI PUDJILESTARI SUDARMONO
Microbiology Indonesia Vol. 9 No. 3 (2015): September 2015
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (372.202 KB) | DOI: 10.5454/mi.9.3.4

Abstract

Surfactin is a lipopeptide biosurfactant that show potential biomedical application due to its activities such as antiviral, antibacterial, antifungi, anticancer, and antimycoplasma. Bacillus amyloliquefaciens MD4-12, isolated from oil-contaminated soil, produced promising yield of surfactin in McKeen medium. The production of surfactin was influenced by many fermentation process parameters such as carbon, nitrogen, minerals and also environmental conditions such as pH and agitation. Therefore, to obtain high yield of surfactin by Bacillus amyloliquefaciens MD4-12, optimization of process production was conducted in shake flask fermentation using response surface methodology. McKeen medium composition was used as basal medium.  Screening of the best carbon and nitrogen source were selected in preliminary experiments followed by selection of the influencing significant parameters on surfactin production using Plackett-Burman design. Selected parameters were optimized by central composite design and for the data analysis was used response surface methodology. The result showed that the optimum medium composition contained (g/L) 45.0 glucose, 6.33 urea, 1.0 monosodium glutamate, 1.85 MgSO4.7H2O, 0.4 KCl, 0.5 K2HPO4 and 0.5 mL trace elements. The surfactin yield at optimal condition was 1.25 g/L, increased 2.4 times compared to condition prior to optimization. 
Determination of blaVIM and blaIMP Resistant Genes againts Meropenem of Pseudomonas aeruginosa Isolated from HCU Bronkopneumona Inpatients at Internal Medicine RSUP dr M Djamil Padang RINGGA NOVELNI; MARLINA MARLINA; RAVEINAL RAVEINAL
Microbiology Indonesia Vol. 9 No. 3 (2015): September 2015
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (205.993 KB) | DOI: 10.5454/mi.9.3.5

Abstract

In this research, we aimed to detect blaVIM and blaIMP the resistant genes against Meropenem in Pseudomonas aeruginosa from sample of hospitalization patients at the Internal Medicine HCU of RSUP DR. M. Djamil Padang. Firstly, bacterial isolate of P. aeruginosa were isolated from the sputum samples of patients who suffered bronkopneumonia. The isolation were started with samples cultivation to the Cetrimide Agar media which was a selective media for P. aeruginosa. To determine the species of the bacteria, the identification using Gram staining, Triple Sugar Iron Agar (TSIA) test, citric test, urease test, Methyl Red/ Voges–Proskauer (MR/VP) test, and molecular marker of 16S rRNA genes have been conducted. The isolation and identification result showed that from 20 sputum samples of the patients there were just 10 (50%) samples were positively containing P. aeruginosa. From the P. aeruginosa isolates, the resistant genes against meropenem blaVIM and blaIMP were amplified using PCR. The result showed that all these P. aeruginosa isolates have positively genes encoding for Metallo-β-Lactamase (MBLs).
ITA registration form and Back Cover Iman Rusmana
Microbiology Indonesia Vol. 9 No. 3 (2015): September 2015
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (961.495 KB) | DOI: 10.5454/mi.9.3.%p

Abstract

Orchid Mycorrhizal Fungi: Identification of Rhizoctonia from West Kalimantan ROSA SURYANTINI; REINE SUCI WULANDARI; RINA SRI KASIAMDARI
Microbiology Indonesia Vol. 9 No. 4 (2015): December 2015
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2256.142 KB) | DOI: 10.5454/mi.9.4.3

Abstract

Orchid is an ornamental plants with high economic value. The excessive exploitation of orchids threatened or even endangered the species, especially those of the epiphytic orchids (Appendiculla sp., Calanthe vestita, and Bulbophyllum beccarii) in West Kalimantan. The discovery of the interaction between orchids and mycorrhizal fungi raises the possibility of ex situ conservation of orchids and it will ensure the success of orchid conservation. Orchid mycorrhizal fungi belongs to the group of Rhizoctonia-like,in which comprised of different genus such as Ephulorhiza, Ceratoriza, and Tullasnela. So far, there is no report on the identit of orchid mycorrhiza associated with the epiphytic orchids in West Kalimantan. The purpose of this study was to identify Rhizoctonia-like associated with Appendiculata sp., Calanthe vestita, and Bulbophyllum beccarii roots in the forest of Raya Pasi and Gunung Bawang, West Kalimantan. The methods were isolation and identification of Rhizoctonia-like from healthy orchid's root based on their morphological characteristics (such as the colony colour, hyphal cell size, sclerotial, concentric circles and monilioid cell, number of nuclei per cell), observation of peloton in root tissue and grouping of isolates. Based on identification of orchid mycorrhiza on the roots of the three species of orchids from West Kalimantan, it was observed that Ceratorhiza sp. was associated with Appendiculla sp.,Ephuloriza sp. with C. vestita, and Tullasnela sp. with B. beccarii roots, respectively. This result is preliminary information and it is still need to be further studied, especially on the role of Rhizoctonia-liker as orchid mycorrhizal fungi in association with the epiphytic orchid for conservation. 
Heavy Metals Biosorption by Copper Resistant Bacteria of Acinetobacter sp. IrC2 WAHYU IRAWATI; ADOLF JN PARHUSIP; NIDA SOPIAH
Microbiology Indonesia Vol. 9 No. 4 (2015): December 2015
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2817.263 KB) | DOI: 10.5454/mi.9.4.4

Abstract

 Heavy metal pollution is a serious problem because it cannot be degraded by natural processes and persist in soil, water, and sediment. Many indigenous microorganisms isolated from heavy metal contaminated sites had tolerance to heavy metals toxicity and could be used for bioremediation agent because of its capability to biosorb heavy metals. The aim of this research was to study the potency of copper resistant bacteria Acinetobacter sp. IrC2 as a biosorbent of heavy metals. Biosorption was determined by measuring the heavy metals concentration on growing medium by using atomic absorption spectrophotometer. The research showed that Acinetobacter sp. IrC2 was capable of growing in medium containing each of 2 mM of copper, zinc, lead, cadmium, and the mixture of those heavy metals. The addition of copper and lead in the medium changed morphological appearance of colonies to green and brown, respectively, suggesting that the survival mechanism of the isolate was by biosorbing copper or lead inside the cells. The percentage of heavy metals biosorption efficiency using live cells of Acinetobacter sp.IrC2 were up to 64.31% of copper, 24.73% of zinc, 62.79% of lead, and 11.56% of cadmium. Acinetobacter sp. IrC2 also reduced copper, lead, and cadmium concentration up to 24.30, 75.93, and 16.38%, respectively, in medium supplemented with 1 mM of the mixture of these heavy metals. The findings of this study indicated that Acinetobacter sp. IrC2 was a promising bacterium for removal of heavy metals.

Page 12 of 40 | Total Record : 398

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