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Microbiology Indonesia

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Published by Perhimpunan Mikrobiologi Indonesia

ISSN : 19783477 EISSN : 20878575 DOI : -

Core Subject : Health, Science,

Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology

Microbiology Indonesia provides a unique venue for publishing original researches in microbiology (espesially from Indonesian reseachers), and ensures that authors could reach the widest possible audience. Microbiology Indonesia publishes a wide range of research disciplines on bacteria, archaea, fungi, protozoa, and virus as well as biotechnology related to microbiology. Topics include (but are not limited to): -methods in microbiology, -bioprocess, -environmental microbiology, -food microbiology, -plant-microbe interaction, -animal-microbe interactions, -microbial community, -microbial genetics, -virology, -comparative and functional microbial genomics, -and gene expression in microbes.

Arjuna Subject : Imunologi dan Mikrobiologi (semua kategori) - Mikrobiologi dan Bioteknologi Terapan

Articles 398 Documents

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Enhancing the Removal of Highly Concentrated CO2 Through Synergism between Microalgae Consortium and Nutrient Ratio in Photobioreactor
Microbiology Indonesia Vol. 10 No. 3 (2016): September 2016
Publisher : Indonesian Society for microbiology

This research was carried out by developing the carbon capture and storage (CCS) technology to determine the synergism between microalgae consortium and the optimum nutrient ratio as an effort to obtain higher CO2 removal efficiency and CO2 utilization efficiency. The microalgae consortium consisting of Chlorella sp., Scenedesmus sp. and Ankistrodesmus sp. have been selected previously as potential candidates for Microbial Carbon Capture and Storage (MCCS) agent and already cultured continuously in PHM (Provasoli Haematococcus Media) artificial medium, in vertical column photobioreactor. Pure CO2 gas at a high concentration of 10% (v/v) flowed from the bottom of vertical column photobioreactor continuously with optimum flow rate of 5 L.min-1. A growth medium (PHM) containing artificial nutrients was flowed continuously at flow rate 7L.day-1 and detention time 3.8 days. Four fluorescent lamps were positioned outside the photo-bioreactor to obtain light intensity of 4000 lux, set for 16 hours light exposure and 8 hours dark, with operating temperature 30Â°C maintained during the study. Three compositional variations of microalgae consortium were used. They are as follows; Â Ch : Sc : An = 1: 1: 1; Ch : Sc = 1: 1; and Ch : An = 1: 1, where Ch, Sc, and AnÂ wereÂ Chlorella sp., Scenedesmus obliquus and Ankistrodesmus sp., respectively. The following variations of nutrient composition were used; C: N: P = 100: 10: 1, C: N: P = 100: 50: 1 and C: N: P = 100: 25: 1. The C, N, and P sources were CO2 (inorganic), KNO3, and KH2PO4, respectively. This study proves that synergism between the types making up the consortium also determined the ability to utilize inorganic carbon source. Without the presence of Ankistrodesmus sp., synergism between Scenedesmus obliquus and Chlorella sp. showed twice higher CO2 utilization efficiency in comparison to the synergism between Ankistrodesmus sp and Chlorella sp. Increased nitrogen concentration in medium increased the growth of Chlorella sp and Scenedesmus obliquus as a consortium, the CO2 removal efficiency, the CO2 utilization efficiency and the Carbon Uptake Rate. The nutrient ratios C:N:P of 100:50:1 could increase CO2 utilization efficiency upto 50% higher than the C:N:P of 100:10:1.

Screening, Purification, and Characterization of Cellulase from Fungi Isolated from Used Mushroom Substrate IWAN SASKIAWAN; NUR HASANAH
Microbiology Indonesia Vol. 9 No. 4 (2015): December 2015
Publisher : Indonesian Society for microbiology

Alarge number of microorganism especially filamentous fungi has ability to degrade cellulose. The purpose of this study was to conduct screening, purification, and characterization of cellulase from fungi which was isolated from used paddy straw mushroom substrate. Screening of cellulytic activity using CMC medium shown that 11 out of 20 isolates of fungi produced a clearing zone surrounding fungal colony. Among them isolate number JMF 12 showed the highest cellulase activity and was further used for purification and characterization. The cellulase was purified to electrophoretical homogeneity by ammonium sulfate precipitation, dialyzed by Novagen D-Maxi TubeTM Dialyzer, and Sephadex G-100 gel filtration chromatography. The recovery and purification fold was 3.82 % and 1.98 respectively, after Sephadex G-100 gel filtration chromatograph. The o purified cellulase had an optimal pH and temperature at 6 and 45 C.Â The Km and Vmax of cellulase was 11.43 mM and 0.006mmol/min respectively. The purified cellulase was activated by Na+ and Zn++ but inhibited by Ca++, Co++, Fe++, andÂ Hg++ .

The Utilization of Modified Cassava Flour (Mocaf) Industry Waste and Peat as Carrier of Nitrogen-Fixing Bacteria and Phosphate Solubilizing Bacteria Inoculant RETNO ROSARIASTUTI; SUMANI SUMANI; SUPRIYADI SUPRIYADI; MUHAMMAD ARDIAN NURSETYAWAN; PRAMUSITA YOGA DANISWARA
Microbiology Indonesia Vol. 11 No. 4 (2017): December 2017
Publisher : Indonesian Society for microbiology

Fertilizer demand in Indonesia increase for increasing agricultural production.Biofertilizer is organic fertilizer with the addition of specific microorganisms which are expected to reduce the amount of inorganic fertilizer. Carrier material plays an important role in maintaining the viability and storage period. Peat is used as a biofertilizer carrier forÂ a long time. Solid waste of Modified Cassava Flour (Mocaf) which is the remainder of mocaf industry has great potential as a carrier material of good biofertilizers,Â because of its nutrient content. The aim of this study wasÂ determining the potential of mocaf solid waste and its combinations with peat as the carrier in supporting the growth of Nitrogen-fixing Bacteria (NFB) and Phosphate Solubilizing Bacteria (PSB) during the incubationÂ of microorganisms. The experiment was conducted at the Laboratory of Soil Biology and Biotechnology, Faculty of Agriculture of Sebelas Maret University (UNS) using completely randomized design (CRD) with two factors of a carrier and incubation time as the experimental design. ThereÂ wereÂ threeÂ types ofÂ carrierÂ whichÂ have differentÂ combination. The base material were solidÂ wasteÂ ofÂ MocafÂ industryÂ Â andÂ peat.Â All materialsÂ of carrierÂ wereÂ mixed and sterilized, than inoculated by Nitrogen- FixingÂ Bacteria (NFB)Â andÂ Phosphate SolubilizingÂ Bacteria (PSB) and incubatedÂ forÂ 60Â days. TheÂ growth ofÂ bacterias were analyzedÂ every 15Â daysÂ andÂ theÂ chemical compositionÂ ofÂ carrierÂ wereÂ analyzedÂ atÂ the begin and theÂ end ofÂ researchÂ Â (incubation).Â Â Â The results indicated that the incubation time significantlyaffected viability of NFB and PSB. Until 60th day incubation time, still showed the increasing growth of NFB and PSB.

Detection of Hepatitis B Virus X Gene Mutation from Local Clinical Samples ANITA ARTARINI; HANARY G JESSICA; RADEN RINI KARTIKASARI; CATUR RIANI; DEBBIE S RETNONINGRUM
Microbiology Indonesia Vol. 10 No. 1 (2016): March 2016
Publisher : Indonesian Society for microbiology

Hepatitis B virus (HBV) belongs to the Hepadnaviridae family and it infects hepatocytes, which is the most common cell in liver. HBV infection is distinguished into acute and chronic infection based on the duration of infection. Chronic infection of HBV exists more than six months and it can develop into liver cirrhosis and hepatocellular carcinoma (HCC). The development of chronic HBV infection is affected by viral particle load, genotype and subgenotype, as well as its association with HBV X gene mutation. The aim of this study is to determine the genotype and subgenotype based on X gene, and to detect its mutation. The X gene in DNA samples was amplified with nested PCR, which produced two fragments with size of 469bp and 395bp. The DNA sequencing results of the amplified product were analyzed by BLAST program to determine their HBV genotype and subgenotype based on the X gene sequences. The analysis showed that patients were infected mainly by HBV subgenotype B3, which are common in Indonesia. The obtained HBx gene sequences were aligned with HBx gene sequences from database to determine the genotype, subgenotype and amino acid substitution. In this study, we described the presence of known HCC-related HBx mutant, i.e. V5L, A47T, I127T and K130M/V131I, as well as new HBx mutant, i.e. G22S and A85T. The presence of HBx T118N mutant was detected at the highest percentage and occurred from samples with high HBV DNA titer.Â

Utilization of the Bacteria Bacillus pumilus and Citrobacter youngae as Flotation Bioreagents in the Microflotation of Chalcopyrite, Pyrite, and Silica
Microbiology Indonesia Vol. 10 No. 1 (2016): March 2016
Publisher : Indonesian Society for microbiology

Until presently, relatively toxic and expensive chemical reageants are routinely used in the flotation of sulfide and oxide minerals. To establish a more environmentally friendly flotation process, alternative flotation reagents have been explored extensively by using microbes and their metabolic products such as biosurfactants or EPS (extracellular polymeric substances) as high molecular weight biosurfactants. Hence, the present work focused on the application of the mixotrophic bacteria capable of both producing biosurfactants and oxidizing iron-sulfur (herein Bacillus pumilus strain SKC-2 and Citrobacter youngae strain SKC-4) as flotation bioreagents in the microflotation of chalcopyrite, pyrite and silica. Laboratory microflotation tests using both bacterial strains as bioreagents were evaluated as a function of conditioning time, pH and bacterial cell concentration. Experimental evidence indicated that the chalcopyrite recoveries could be achived using both bacterial strains but its better recovery was obtained with the bacterium Citrobacter youngae as bioreagents. The findings of this study thus suggest the possible application of these bacterial strains as flotation bioreagents in order for establishing a more eco-friendly mineral processing.

Screening of Antibiofilm Activity from Marine Bacteria against Pathogenic Bacteria ALIANDA BUDHIRIANi ROSSATI CAMESI; AGUSTINA LUKITO; DIANA ELIZABETH WATURANGI; HWANG JAE KWAN
Microbiology Indonesia Vol. 10 No. 3 (2016): September 2016
Publisher : Indonesian Society for microbiology

Bacterial biofilms produced by pathogenic bacteria have become a serious issue in several chronic diseases such as atherosclerosis, cystic fibrosis, endocarditis, inner ear infections, and kidney stones. Thus, inhibition and destruction of bacterial biofilm from pathogenic bacteria is needed. The purpose of this study is to analyze biofilm inhibition and destruction activities of marine bacteria associated with hard and soft corals isolated from several oceanic regions in Indonesia. Fifteen marine isolates collected from several regions in Indonesia such as Bali Province, South East Sulawesi Province, East Java Province, Lampung Province, and Banten Province were tested using static biofilm assay against several pathogenic bacteria. Biofilm of the pathogenic bacteria tested were stained using 0.4% crystal violet. Several isolates were sequenced using 16S rRNA PCR method. Most of marine isolates presented higher inhibition and or destruction activity at 10% crude concentration. Few isolates were further identified using 16S rRNA and proven to have antibiofilm activity against several pathogenic bacteria. Marine bacteria have broad applications in medical and pharmaceutical industries and the oceanic regions of Indonesia are promising sources for the discovery of novel bacteria with antibiofilm activity.

Comparison of Microbial Pattern Causing Urinary Tract Infection in Female Out- and Hospitalized Patients in Jakarta YEVA ROSANA; DWIANA OCVIYANTI; ANIS KARUNIAWATI; SYADZA RHIZKY PUTRI AKHMAD
Microbiology Indonesia Vol. 10 No. 1 (2016): March 2016
Publisher : Indonesian Society for microbiology

Urinary Tract Infection (UTI) is an infection in any part of the urinary system. Women are 3 times more likely to have UTI than men. The UTI accounts for 15% infection cases in outpatients and 24% cases in hospitalized patients. Although the most common cause of UTI is certain bacteria, but it was not easy to choose the appropriate antimicrobial therapy. Strategy for choosing empiric antimicrobial treatments for UTI in female out- and hospitalized patients should be based on the pattern of the causative organisms. The aim of this study was to understand the microbial pattern causing UTI in female out- and hospitalized patients in Jakarta. The UTI -1 causative microorganisms were obtained from urine culture containing 100,000 cfu/mL . Twenty nine microorganisms were found as the causative agents of UTI in 317 pregnant women who came to six Community Health Centres (Puskesmas) in Jakarta: Makassar; Pulogadung, Cakung, Pasar Rebo, Duren Sawit, and Kramat Jati for antenatal care. Twenty nine microorganisms were isolated from 114 urine samples of female hospitalized patients who were diagnosed of UTI. The samples were obtained from the Microbiology Laboratory Clinic of FKUI-RSCM. The most common microorganisms causing UTI in female out- and hospitalized patients were Gram negative bacteria. In female outpatients, Klebsiella sp was the most common causative bacteria (31%), followed by Escherichia coli (24.1%). In female hospitalized patients, Escherichia coli was the most common causative bacteria (30%), followed by Candida sp (24.1%) and Klebsiella pneumonia (6.8%). There was more variation in the pattern of UTI causative organisms in hospitalized female patients in comparison to that of the outpatients. Candida sp. was only found in hospitalized UTI patients but not in outpatients.

Primary Tupaia javanica Hepatocytes Cultures As In Vitro Replication System for Ape Hepatitis B Viruses MARYATI SURYA; DIAH ISKANDRIATI; SILMI MARIYA; UUS SAEPULOH; PERMANAWATI PERMANAWATI; DONDIN SAJUTHI; JOKO PAMUNGKAS
Microbiology Indonesia Vol. 10 No. 2 (2016): June 2016
Publisher : Indonesian Society for microbiology

Hepatitis B virus (HBV) is a DNA virus with liver as primary target organ.This virus caused chronic infection that can progress to cirrhosis, liver cancer and even death. In vitro model system of hepatocyte cultures is important and widely used to study a variety aspects of hepatitis B. Development of small animal Tupaia sp. for the in vitro model system is an alternative to the existinghepatocyte cultures. The specific purpose of the studyis to develop Tupaia javanica hepatocytes culture for HBV replication, and in a broader spectrum to answer the need for in vitro model of hepatocytes. Primary T. javanica hepatocytes (PTH) culture was successfully maintained for 14 days to reach 80% confluence, and infection of Javan gibbon HBV (GiHBV) and orangutan HBV (OuHBV) onto the culture on day 15 showed viral replication for up to eight days as measured by polymerase chain reaction (PCR). PCR quantification indicated that the highest copy number of DNA virus was detected onday two anddecreased until day 8 after infection. Cell receptor for HBV attachment, known as sodium taurocholate cotransporting polypeptide was expressed on the surface of PTH and shown as green luminenscent when observed by immunofluorescence assay. Sequence of partialS gene from the apes HBVs after the viruses have been infected to the PTH showed amino acid identity to their wildtype as high as 99.29% for GiHBV and 95.71% for OuHBV. This study suggested that the PTH can support the replication of GiHBV andÂ OuHBV.Â

Diversity of Culturable Bacterial in Various Parts of Luwakâ€™s (Paradoxurus hermaprodithus javanica) Gastrointestinal Tract SONY SUHANDONO; HERI SETIADI; TATI KRISTIANTI; ALI BUDHI KUSUMA; ANDINI WARIH WEDARINGTYAS; DEMI TRISTAN DJAJADI; I NYOMAN PUGEG ARYANTHA
Microbiology Indonesia Vol. 10 No. 2 (2016): June 2016
Publisher : Indonesian Society for microbiology

Luwak coffee is a highly-priced coffee produced exclusively by the palm civet or luwak (Paradoxurus hermaphrodites ssp.). The purpose of this study was to determine the diversity of culturable bacteria in the gastro intestinal tract of luwak. The bacterial isolates were phenotypically characterized by their morphology and molecularly by analysis of their1,500bp 16s rDNA sequence. The results showed that Enterobacter cloacae and Lactobacillus brevis were found all over luwakâ€™s digestive tract. Enterobacter cloacae was the most common species. The most diverse bacterial population was found in small intestine. Seven bacterial generawere successfully identified from the small intestine and colon, compared to only five genera found in the stomach.

Nitrogen Fixing Potential of Endophytic Bacteria Isolated from Aloe barbadensis Miller and Aloe sp.
Microbiology Indonesia Vol. 10 No. 2 (2016): June 2016
Publisher : Indonesian Society for microbiology

Aloe is a crassulacean acid metabolism (CAM) species that are known to live in extreme enviroment such as drought condition. Nitrogen fixation procces influenced by the ability of plants to adapt in drought condition. Endophytic bacteria from Aloe and their ability for nitrogen fixation were little reported, but potential and its relationship between the ability for nitrogen fixing with resistance to drought conditions have not been reported. This research aimed study the endophytic bacteria from two varieties of aloe, namely Aloe barbadensis Miller and Aloe sp. in their ability on conducting the nitrogen fixing process and its relationship with resistance to drought. Characterization of endophytic bacteria were carried out by morphological observation of colony, Gram staining and molecular identification. Screening of nitrogen fixation was done using nitrogen-free semisolid NFb malate medium. Endophytic bacteria from Aloe sp. more than A. barbadensis in their potency of nitrogen fixation which related with habitat where their planted. A total of 40% of the endophytic bacteria isolates from the leaves of the aloe var. A. barbadensis and 62.5% of isolates from var. Aloe sp. are known to have a better ability to fixing nitrogen than the others. Isolates A barbadensis AB 12 and Aloe sp. AS 8 were the best isolates from each varieties on ability for nitrogen fixation. Based on 16S rRNA gene analysis those two selected isolates belonged to Bacillus methalotropicus strain DA 16-5 and Bacillus aryabhattai strain B8W22.Â

Page 13 of 40 | Total Record : 398

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Iman Rusmana

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kPERHIMPUNAN MIKROBIOLOGI INDONESIA (SeKretariat PERMI), Gedung 10.2 Indonesian Life Sciences Center (ILSC), Zona Bisnis Teknologi Puspiptek, Jalan Raya Serpong - Bogor Gunung Sindur, Jawa Barat 16340, Indonesia. Email: microbiology.indonesia@gmail.com

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Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name Iman Rusmana

Contact Email rusmana13@yahoo.com

Phone +62217560536

Journal Mail Official microbiology.indonesia@gmail.com

Editorial Address kPERHIMPUNAN MIKROBIOLOGI INDONESIA (SeKretariat PERMI), Gedung 10.2 Indonesian Life Sciences Center (ILSC), Zona Bisnis Teknologi Puspiptek, Jalan Raya Serpong - Bogor Gunung Sindur, Jawa Barat 16340, Indonesia. Email: microbiology.indonesia@gmail.com

Location Kota tangerang, Banten INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
Iman Rusmana

Contact Email
rusmana13@yahoo.com

Phone
+62217560536

Journal Mail Official
microbiology.indonesia@gmail.com

Editorial Address
kPERHIMPUNAN MIKROBIOLOGI INDONESIA (SeKretariat PERMI), Gedung 10.2 Indonesian Life Sciences Center (ILSC), Zona Bisnis Teknologi Puspiptek, Jalan Raya Serpong - Bogor Gunung Sindur, Jawa Barat 16340, Indonesia. Email: microbiology.indonesia@gmail.com

Location
Kota tangerang,

Banten

INDONESIA