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The Journal of Experimental Life Sciences (JELS)
Published by Universitas Brawijaya
ISSN : 20872852     EISSN : 23381655     DOI : 10.21776/ub.jels
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Neuroscience
The Journal of Experimental Life Science (JELS) is a scientific journal published by Postgraduate School, University of Brawijaya as distribution media of Indonesian researcher’s results in life science to the wider community. JELS is published in every four months. JELS published scientific papers in review, short report, and articles in Life Sciences especially biology, biotechnology, nanobiology, molecular biology, botany, microbiology, genetics, neuroscience, pharmacology, toxicology, and Applied Life Science including fermentation technology, food science, immunotherapy, proteomics and other fields related to life matter. JELS is a scientific journal that published compatible qualified articles to the academic standard, scientific and all articles reviewed by the expert in their field. The Journal of Experimental Life Science (JELS) have a vision to become qualified reference media to publish the best and original research results and become the foundation of science development through invention and innovation on cellular, molecular, nanobiology, and simulation work related to life matter rapidly to the community. The Journal of Experimental Life Science (JELS) has objectives to published qualified articles on research’s results of Indonesian researchers in life science scope. JELS encompasses articles which discuss basic principles on natural phenomenon with cellular, molecular, and nanobiology approach.
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 6 Documents
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Search results for , issue "Vol. 16 No. 1 (2026)" : 6 Documents clear
Multidrug-resistant Bacteria in Domestic Cats (Felis catus): A Global Health Threat Emerging from Pets Mumtaza, Furzania; Jatmiko, Yoga Dwi; Salsabila, Maudy Syifa; Faturohmah, Yorda Nur; Fauziah, Ima; Saputra, Sugiyono
The Journal of Experimental Life Science Vol. 16 No. 1 (2026)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2026.016.01.01

Abstract

Cats (Felis catus) act as potential reservoirs for pathogenic bacteria due to their close contact with humans. Despite growing concerns about antimicrobial resistance (AMR) and zoonotic transmission, data on the diversity of antimicrobial-resistant bacteria, especially Staphylococcus aureus, in cats in Indonesia are limited. This study aimed to identify indicator bacteria in domestic cats and characterize their antimicrobial resistance (AMR) profiles. Nine earlobe swab samples were collected from cats at the Splendid Animal Market and Jalan Simpang Madukoro, Malang, in January 2023. White-toyellow colonies with  hemolytic activity formed on blood agar. Six antibiotics are used to asses antimicrobial susceptibility. Based on the European Committee on Antimicrobial Susceptibility Testing (EUCAST) breakpoints, all isolates (100%) were resistant to rifampicin; seven isolates (78%) to oxacillin and cefoxitin; six isolates (67%) to gentamicin; four isolates (44%) to ceftriaxone; and one isolate (11%) to ciprofloxacin. Seven isolates (P1D, P3, P5, P7, R3, R5, and R9) were  resistance to more than three antibiotic classes were classified as multidrug-resistant bacteria (MDR). Molecular identification using 16S rRNA gene sequencing and phylogenetic analysis revealed the isolates as Chryseobacterium sp. (P1D), Enterobacter hormaechei (P3), Acinetobacter sp. (P5), Enterobacter cloacae (P7), Exiguobacterium sp. (P9A), Staphylococcus sciuri (R1A), Enterococcus hirae (R3), Pantoea sp. (R5), and Stenotrophomonas maltophilia (R9). The detection of ceftriaxone resistance and the presence of MDR bacterial strains indicate that cats carry resistant bacteria that may impact public health.
The Effects of the Coating Materials Ratio on the Characteristics of Encapsulated Red Beet Extract Maskurin, Quinnike Aisy; Septiafianti, Niar; Fitriah, Arsinah Habibah; Widyaningsih, Tri Dewanti; Mubarok, Ahmad Zaki
The Journal of Experimental Life Science Vol. 16 No. 1 (2026)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2026.016.01.06

Abstract

Food products with beneficial health effects are increasingly attracting public interest, in line with growing health awareness. Red beetroot (Beta vulgaris L.) is one such food that contains many bioactive compounds and exhibits high antioxidant activity. However, the bioactive compounds in red beetroot are susceptible to degradation, leading to color fading, reduced antioxidant capacity and bioavailability, and shorter shelf life during processing and storage. To enhance their stability, a microencapsulation process using specific coating materials can be applied. This study optimized the wall-material ratio for spray-drying beetroot extract microcapsules, prioritizing the stability/retention of bioactives, encapsulation efficiency, and instant solubility. Microcapsules were produced with soy protein isolate (SPI) : maltodextrin (MD) ratios of 0:1, 1:3, 3:7, 2:3, and 1:1 at a total wall concentration of 10% (w/w) using spray-drying under fixed conditions, inlet temperature 150°C; outlet temperature 82°C; feed rate 5.1 mL·min⁻¹. Betalains were quantified spectrophotometrically; total phenolics by Folin–Ciocalteu; total flavonoids by the aluminum-chloride method; and antioxidant activity by DPPH. Encapsulation efficiency was calculated as the ratio of retained betalains to the initial amount. Microencapsulation of red beet extract using soy protein isolate (SPI) and maltodextrin (MD) as wall materials significantly affected the physicochemical and functional properties of the microcapsules. Antioxidant activity, flavonoid content, phenolic compounds, and encapsulation efficiency increased when SPI and MD were combined at a 1:1 ratio.
A Comparative Analysis of Leaf Trichomes in Diploid and In Vitro-Induced Tetraploid Patchouli (Pogostemon cablin Benth.) Aini, Qurrotu; Widoretno, Wahyu
The Journal of Experimental Life Science Vol. 16 No. 1 (2026)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2026.016.01.02

Abstract

This study aimed to analyze the types, sizes, numbers, and densities of leaf trichomes in in vitro–induced tetraploid patchouli (Pogostemon cablin Benth.) plants and their diploid counterparts. Microscopic observations were conducted on four-month-old leaves obtained from stem cuttings. Both diploid and tetraploid patchouli possessed non-glandular trichomes of the simple unicellular and multicellular types, as well as glandular trichomes of the short- and long-stalked capitate and peltate types. The density of non-glandular trichomes was higher on the abaxial surface of the leaves than on the adaxial surface. Overall, tetraploid plants, particularly genotypes T2, T4, T5, and T7, exhibited higher non-glandular trichome densities than diploids, while the others showed higher densities but were not significantly different. Glandular trichomes of tetraploids were larger, although their numbers were comparable to those of diploids. The head cell diameters of capitate and peltate trichomes were generally larger in tetraploids. These findings indicate that genome duplication enhances trichome development in patchouli, leading to increased secretory capacity and improved structural defense potential against herbivores and pathogens.
Application of Maggot Cultivation Technology to Strengthen Competitiveness of Sustainable Food Micro, Small, and Medium Enterprises Kastaman, Roni; Syahmurman, Faizal; Rahmah, Devi M.; Ardiansah, Irfan; Kurniawan, Koko Iwan Agus; Januardi, Januardi; Puspita, Puspita; Nurliasari, Dessy; Hanifah, Aisyah
The Journal of Experimental Life Science Vol. 16 No. 1 (2026)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2026.016.01.04

Abstract

The cultivation of maggots through the larvae of black soldier fly (Hemetia illucens) is increasingly recognized as a viable solution for organic waste management and alternative protein production. However, its integration into village-based enterprises and implications for Micro, Small and Medium Enterprises (MSMEs) competitiveness remain underexplored. This study examines how maggot cultivation strengthens food MSMEs using a circular economy framework and SWOT (strengths, weaknesses, opportunities, and threats) analysis. The study was conducted at Village-Owned Enterprises (Badan Usaha Milik Desa and abbreviated by BUMDes in Bahasa Indonesia) Kerta Setra Utama in Tasikmalaya, Indonesia. A mixed-methods approach was applied, combining qualitative observation with quantitative analyses of waste reduction, maggot yield, and protein content. Results showed a 65% reduction in organic waste within 5 days, a 28% increase in maggot biomass across 6 production cycles, and a 30% increase in household incomes. Using poultry manure as a substrate reduced waste by 31.2%. Furthermore, the substrate resulted in 114 g of dry weight.kg-1 of feed as well as 25.26% crude protein in less than eight days. SWOT analysis identified key constraints in community participation, institutional support, and technological capacity. There were opportunities that came from the integration of the circular economy and the expansion of green markets. The findings demonstrate that rural enterprises can effectively transform organic waste into economic value through maggot farming. Policy recommendations emphasize capacity building, green financing, and digital market integration to enhance scalability and long-term impact.
Effectiveness of Irrigation Water Conservation in Tomato Cultivation with the Addition of Cattle, Poultry or Goat Manure-Based Compost Andriyani, Idah; Istiqomah, Nuril; Wahyuningsih, Sri; Lestari, Ning Puji
The Journal of Experimental Life Science Vol. 16 No. 1 (2026)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2026.016.01.03

Abstract

Efficient use of irrigation water is essential because water availability often does not meet crop requirements. In response, compost application is a potential solution, as it improves soil structure and enhances water-retention capacity. Nevertheless, the water-binding capacity of compost varies depending on the source of animal manure. To address this, the study aimed to evaluate the effectiveness of compost made from cattle, poultry, and goat manure in improving water retention in Andosol soil and supporting tomato growth as a biological indicator. The study used Andosol soil mixed with compost at application rates of 25% (500 g), 50% (1000 g), and 75% (1500 g), along with a control without compost. A two-factor completely randomized design with three replications was applied, and measured parameters included moisture content, media weight, water-holding capacity, pH, plant height, and leaf number. Results showed that the treatment using 1500 g of goat manure compost combined with 500 g of Andosol soil produced the highest water-holding capacity, making it the most effective option for irrigation water conservation. In contrast, cattle manure compost yielded the best tomato growth and productivity. Overall, these findings demonstrate that selecting the appropriate manure-based compost can enhance soil water retention and improve irrigation efficiency.
The Effect of Soaking Temperature on the Kinetic Model of Rehydration of Dried Oyster Mushrooms (Pleurotus ostreatus) Maharani, Dewi Maya; Muhammady, Iqbal Harzulmi; Ubaidillah, Ubaidillah; Hawa, La Choviya
The Journal of Experimental Life Science Vol. 16 No. 1 (2026)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2026.016.01.05

Abstract

Oyster mushrooms (Pleurotus ostreatus) pack a powerful nutritional punch. However, they spoil easily and require special handling for dehydration. Temperature during rehydration is a key factor affecting the quality recovery of mushrooms. Yet, there are limited studies on rehydration kinetics at different temperatures. The principal objective of this study was to investigate how soaking temperatures (25±1°C, 40±1°C, 50±1°C, 60±1°C, and 70±1°C) affect rehydration ratio, moisture content, water absorption rate, and volume expansion ratio at 120 minutes, with three repetitions. The results showed that physical recovery was dominant at lower temperatures (25–40°C). Maximum moisture content (0.850 g.g-1) was achieved at 40°C. In contrast, the optimal rehydration ratio (6.549) occurred at 25 °C. High temperatures (60–70°C) increased the kinetic rate of water absorption, reaching a peak at 60±(0.081g.g-1 min). Volume expansion reached its maximum at 70 °C (1.482). Based on mathematical modeling results, the Midilli-Kucuk model had the highest predictive accuracy at 25°C and 60°C. The Two-Term model captured rehydration phenomena better at 50°C and 70°C. The Bidkhori-Karizaki model provided the most precise data fit when applied at 48°C. Using high temperatures, such as 70°C, can significantly accelerate rehydration kinetics. However, there are technical consequences, including high total energy consumption and risk of structural damage to the product. These effects can reduce its final quality, which is critical for industrial-scale implementation. Therefore, to balance efficiency and product quality, a soaking temperature range of 25–40 °C is recommended as the most optimal operational condition.

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