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The Journal of Experimental Life Sciences (JELS)

jels
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Published by Universitas Brawijaya

ISSN : 20872852 EISSN : 23381655 DOI : 10.21776/ub.jels

Core Subject : Health, Science,

Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Neuroscience

The Journal of Experimental Life Science (JELS) is a scientific journal published by Postgraduate School, University of Brawijaya as distribution media of Indonesian researcher’s results in life science to the wider community. JELS is published in every four months. JELS published scientific papers in review, short report, and articles in Life Sciences especially biology, biotechnology, nanobiology, molecular biology, botany, microbiology, genetics, neuroscience, pharmacology, toxicology, and Applied Life Science including fermentation technology, food science, immunotherapy, proteomics and other fields related to life matter. JELS is a scientific journal that published compatible qualified articles to the academic standard, scientific and all articles reviewed by the expert in their field. The Journal of Experimental Life Science (JELS) have a vision to become qualified reference media to publish the best and original research results and become the foundation of science development through invention and innovation on cellular, molecular, nanobiology, and simulation work related to life matter rapidly to the community. The Journal of Experimental Life Science (JELS) has objectives to published qualified articles on research’s results of Indonesian researchers in life science scope. JELS encompasses articles which discuss basic principles on natural phenomenon with cellular, molecular, and nanobiology approach.

Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)

Articles 12 Documents

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Search results for , issue "Vol. 6 No. 1 (2016)" : 12 Documents clear

Estrous Cycle Response in Mice (Mus musculus) with CSE (Crude Sperm Extract) Injected Intraperitoneally Enni Mutiati; Sri Rahayu; Gatot Ciptadi; Moch. Nasich
The Journal of Experimental Life Science Vol. 6 No. 1 (2016)
Publisher : Postgraduate School, Universitas Brawijaya

Sperm protein has an important role in fertilization process. It becomes antigenic when it is injected to body and can increase TNF-α secretion. TNF-α in blood vesssel can inhibit estradiol synthesis. Estradiol has a significant role in repduction cycle, especially in estrous cycle. The study aims to understand the influence of Crude Sperm Extract (CSE) to mice (Mus musculus) estrous cycle. 16 fertile mices strain Balb-C aged 3-4 months, weighed 20-30 g was divided into 4 groups. P0 is a control group injected by PBS, group P1, P2, and P3 injected by CSE with 1.5 mg.ml-1, 2 mg.ml-1, and 2.5 mg.ml-1. CSE is injected intraperitoneally during mice's diestrus phase. CSE is injected in day 0, day 12, day 24 and observed daily. The data are descriptively analyzed. The results show that CSE with molecule weight between 26.8-176.8 kDa influences estrous cycle. Keywords: Crude Sperm Extract, estrous cycle, folliculogenesis, Mus musculus

Zoonotic Potential of Rotavirus from Swine and Bovine in South of Taiwan Dewi Murni; Pratiwi Trisunuwati; Ming Hui Liao
The Journal of Experimental Life Science Vol. 6 No. 1 (2016)
Publisher : Postgraduate School, Universitas Brawijaya

Rotavirus was recognized as the virus that responsible for causing acute gastroenteritis, especially young livestock. Taiwan Center for Disease Control (CDC) confirms the majority cases of acute gastroenteritis in Taiwan on February 2015 were caused by rotavirus. In this study, we report the incidence and zoonotic impact of rotavirus strain from Taiwan. This study examined 90 (swine) and 60 (bovine) fecal samples collected from south of Taiwan in March 2015. Detection of rotavirus using VP6 gene by RT-PCR technique with amplicons 379 bp. Zoonotic potential analysis based on nucleotide sequence and phylogenetic analysis. RT-PCR utilizing the primers specific for VP6 gene detected rotavirus with positive reactions 3/30 (10%) in piglets and 1/20 (5%) in the calf. Based on the nucleotide sequences and phylogenetic analysis indicated that 1 of 3 wild strains from swine rotavirus had 85.0% - 91.1% and 1 wild strain from bovine had 78.7% - 85.9% identity relations with human strains. These findings indicated that the wild strains of swine and bovine rotavirus may broadly spread and contribute to zoonotic transmission. Keywords: Bovine, Rotavirus, RT-PCR, Swine, Zoonotic.

Oocyte In Vitro Maturation with Crude Sperm Extract Protein of Bull's Spermatozoa Bilqis Bilqis; Sri Rahayu; Gatot Ciptadi
The Journal of Experimental Life Science Vol. 6 No. 1 (2016)
Publisher : Postgraduate School, Universitas Brawijaya

Oocyte In vitro maturation (IVM) is one of the important parts for in vitro fertilization (IVF). The success of oocyte maturation is influenced by the composit ion and the quality of IVM medium. Culture medium which used to IVM not only influences the oocyte process to reach metaphase II and proceed the fertilization, but also influences to developmental of an embryo. Crude sperm extract has high-level protein kinase and contains some sperm proteins. Crude sperm extracts expected as natural maturation medium that can increase the success of In Vitro Maturation (IVM). The characterization of crude sperm extracts profile with sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Oocyte maturation is observed in the 26th hour from the first culture. The result of crude sperm extract characterization obtained from the protein with the molecular weight is 176.8, 63.2, 58.4, 55.3, 52.4, 49.7, 44.6, 38.02, 36.03, 34.15 and 26.8 kDa. Oocyte maturation with concentration of crude sperm extract 1.5 µg.mL-1 with 71.6% matures oocyte and oocyte maturation with concentration of crude sperm extract 2.5 µg.mL-1 with 75% matures oocyte. Keywords: Crude Sperm Extract, In Vitro Maturation, Oocyte

Effectiveness of Indigenous Lead (Pb) Reducing Bacteria Consortia of Waste Water Treatment in Agar Flour Industry Wasiatus Sa'diyah; Endang Suarsini; Ibrohim Ibrohim
The Journal of Experimental Life Science Vol. 6 No. 1 (2016)
Publisher : Postgraduate School, Universitas Brawijaya

Lead (Pb) is known as toxic element in environment. It is produced from processing of Agar Flour industry activities. Enhancement of Pb in the waste water of agar flour is very dangerous for human health. Effect of lead damage some organ e.g. kidney, liver, and hair. Environment standard of lead concentration on waste water based on Governor of East Java Regulation No. 72 of 2013 is 1 mg.L-1, otherwise, initial lead levels of waste water are 3.114 mg.L-1. The aim of the research is reducing the level of lead to be harmless for the environment. One method to decrease a high concentration of lead in waste water is biosorption. Consortia of Bacillus alvei and Bacillus pumilus as indigenous bacteria are used to decrease Pb level in the wastewater. The experiment was carried out with varies of wastewater concentration and bacteria 3 %, 4%, 5%, 6%, and 7%. Consortium of B. alvei dan B. pumilus at 7 % concentration have the highest potency 93.58 % to reduce lead reach 0.2 mg.L-1.Keywords: Bacteria, Consortia, Concentration, Pb, Waste of Agar Flour

Latent and Eggs Production of Banggai Cardinal (Pterapogon kauderni, Koumans 1933) on Various Salinity Levels: Conservation Efforts Atiek Pietoyo; Sri Andayani; Agoes Suprijanto
The Journal of Experimental Life Science Vol. 6 No. 1 (2016)
Publisher : Postgraduate School, Universitas Brawijaya

The aim of this study is to determine the best salinity level on latent and eggs production of Banggai Cardinal (Pterapogon kauderni Koumans 1993) based on first brood and brood production of Banggai Cardinal in the treatment. Water quality measurement was carried out every day to maintain water quality. Water quality measurement showed suitable salinity for Banggai Cardinal breeding. Brood latent showed no significant difference between the treatments. Total of 27 ppt is the level of salinity for Banggai Cardinal reproduction to gain positive respond on eggs production (42.3333 ± 7 eggs). Keywords: Banggai Cardinal, Eggs Production, Latent, Salinity.

Water and Chlorophyll Content and Leaf Anatomy of Patchouli Planlet (Pogostemon cablin Benth.) Resulted by Shoot-tip Culture Experience Hyperhydricity after Treatment of Modification Ammonium nitrate or Macro salt Concentration on MS medium (Murashige Skoog) Aisyah Rahmawati Zen; Wahyu Widoretno; Serafinah Indriyani
The Journal of Experimental Life Science Vol. 6 No. 1 (2016)
Publisher : Postgraduate School, Universitas Brawijaya

Hyperhydricity is a symptom of abnormal morphological and physiological function which inhibits the regeneration of plantlets. In general, the main symptom of hyperhydricity is a change in the condition of the plantlets which looks clear (Glassy) as a result of low levels of chlorophyll, the high water content in the plantlets, and the abnormal anatomical structure of the leaves. Hyperhydricity can be controlled by reducing cytokinin concentration, increasing gelling agent concentration, and reducing ammonium nitrate and macro salt concentration on medium. Objective of this research was to reduce hyperhydricity in shoot tip culture of patchouli by modification of ammonium nitrate and macro salt concentration on MS medium. The various treatment concentrations of ammonium nitrate were 0 mg.L-1 (0), 41.25 mg.L-1 (¼ concentration), 825 mg.L-1 (½ concentration), 1650 mg.L-1 (1 concentration) and macro salt MS with 0, ¼ MS, ½ MS, MS with 5 replications. Hyperhydricity on patchouli shoots could be lowered, as indicated by the decrease in water content from 96% to 90-91%, the increase in total chlorophyll content, and the increased number of palisade cells and stomata on the leaf treatment outcome. The concentration treatment of ammonium nitrate showed better results than the concentration of macros salt in increasing the total chlorophyll content, but it did not differ significantly in lowering water levels and increasing the number of palisade cells and stomata. ¼x concentration treatment of ammonium nitrate could increase chlorophyll content of 0.16 to 0.97 mg.g-1, but MS with 1x concentration showed the best result in the increase of number of palisade cells and stomata of the leaves. Keywords: Ammonium nitrate, Hyperhydricity, Macro Salt, Shoot-tip culture.

The Influence of Fermentation Time in the Physical and Chemical Composition of Fermented Soybean Husk by Using Aspergillus niger on the Quality of Raw Feed Materials Muhammad Ikhwan Ihtifazhuddin; Happy Nursyam; Arning Wilujeng Ekawati
The Journal of Experimental Life Science Vol. 6 No. 1 (2016)
Publisher : Postgraduate School, Universitas Brawijaya

Soybean husk (Glycine max L. Merrill) a soybean processing waste as raw material for tempe obtained after the process of boiling and soaking soybeans. The main problem in the use of soybean husk (Glycine max L. Merrill) as feed material is its crude fiber content which is fairly high. This study aimed to observe the fermented soybean husk using Aspergillus niger to improve the quality of the raw feed materials. This was conducted by using completely randomized design (CRD) analysis and repeated three times; the time optimization of Aspergillus niger in 2, 4, and 6 days based on chemical analyses (moisture, protein, fat, ash, crude fiber and feed containing carbohydrates (NFE) and physical assessment fermentation (smell, texture, moisture and hyphae) were analyzed descriptive qualitatively. The results showed that 4 days fermentation of soybean husk using A. niger is successful gives the highest score based on physical characteristics texture, aroma, moisture, and the formed hyphae and the most effective treatment for decrease in crude fiber is 13% and increase in NFE contained in the largest on 4 days fermented soybean husk by Aspergillus niger with a long time 4 days. Keywords: Aspergillus niger, fermentation, soybean husk.

Effect of Cell Density and Benzyl Amino Purine on the Growth of Somatic Embryo of Citrus Mandarin Batu 55 (Citrus reticulata Blanco.) in Liquid Culture Nawafila Februyani; Wahyu Widoretno; Serafinah Indriyani
The Journal of Experimental Life Science Vol. 6 No. 1 (2016)
Publisher : Postgraduate School, Universitas Brawijaya

Citrus mandarin Batu 55 (Citrus reticulata Blanco.) is one of Indonesian fruits commodities that have high economic value and consumers demand. The propagation of citrus mandarin by plant tissue culture generally was carried out on solid medium. The liquid culture system could increase cell multiplication therefore it became alternative method of plant propagation through somatic embryogenesis. The effect of initial cell density and Benzyl Amino Purin (BAP) concentration in liquid media were investigated. The initial cells density and right concentration of BAP given in media can increase cell proliferation of somatic embryo in liquid culture. Globular somatic embryo were cultured on Murashige and Tucker media with initial cell density 4, 6, 8 and 10 mgL-1 and BAP 0, 0.25, 0.5, and 0.75 mgL-1. Growth evaluation of somatic embryo were obtained by weighing fresh and dry weight every 2 weeks for 8 weeks for initial cell density treatment and 6 weeks of BAP treatment. The result of the research showed that cell density affect the growth of somatic embryo of citrus mandarin. Somatic embryo with low cell density showed slower growth compared than high cell density. Peak growth occured in 6th cultured with cell density 10 mgL-1. In addition to cell density, the growth of somatic embryo in liquid culture was affected by BAP. The growth of somatic embryo on the media containing BAP showed better results than without BAP. The highest BAP concentration on media showed fresh and dry weight of somatic embryo increased. In this research, growth of somatic embryo is not optimal yet because fresh and dry weights of somatic embryo still increase with high concentration 0.75 mgL-1 of BAP.Keywords: Benzyl Amino Purine, cell density, citrus mandarin, liquid medium, somatic embryo.

The Analysis of Hepatopancreas Histologycal Damage in Neocallichirus karumba (Poore and Griffin) Shrimp Caused by Heavy Metal Pb Exposure in Madura Strait Maria Kristiani; Endang Yuli Herawati; Uun Yanuhar
The Journal of Experimental Life Science Vol. 6 No. 1 (2016)
Publisher : Postgraduate School, Universitas Brawijaya

Madura strait known as the second busiest shipping lanes in Indonesia. Human activities on the environment can influence the marine ecosystem derived from the household, fishery processing and capture fisheries. It can pollute coastal environments, one of which is heavy metal exposure such as Cd, Hg, Ca, As and Pb. These metals are greatly impacting the life of sea biota. The objective of this research, therefore, is to examine the hepatopancreas damage rate of shrimp Neocallichirus karumba due to the exposure of heavy metal Pb in Madura Strait. Method of research is by analyzing Pb content in water, sediment and shrimp by taking sample from 3 different stations. Hepatopancreas damage is then analyzed with SEM-EDX. Result of research indicates that at Station A, Pb pollution in water is the biggest and counted for 0.25 ppm, while those in sediment and shrimp are 5.85 ppm and 1.24 ppm. At Station B, Pb pollution in water is 0.19 ppm, whereas that in sediment is 5.51 ppm, but that in shrimp is 1.04 ppm. At Station C, Pb pollution in water counts for 0.18 ppm, and in sediment, it stands for 5.5 ppm but 0.02 ppm for shrimp. Result of analysis against hepatopancreas damage is explained as follows. At Station A, vacuolization is 20 % and Pb content in organ is 0.520 ppm. At Station B, the parameters are 10% and 0.196ppm. At Station C, it includes 15% and 0.173ppm. Organ damage is straightforwardly related to Pb content in water and sediment. Shrimp age is quite influential to the percentage of organ damage.Keywords: Heavy metal Pb pollution, hepatopancreas organ damage, Madura Strait, N. karumba shrimp.

Antimicrobial and Antioxidant Activity of Endophyte Bacteria Associated with Curcuma longa Rhizome Sulistiyani Sulistiyani; Tri Ardyati; Sri Winarsih
The Journal of Experimental Life Science Vol. 6 No. 1 (2016)
Publisher : Postgraduate School, Universitas Brawijaya

Most cases of bacterial resistance towards antibiotics, encourage various efforts to gain new sources of antibiotics. Endophyte bacteria is a microorganism has important role as the producer of bioactive compounds. Endophyte bacteria from Curcuma longa with antimicrobial and antioxidant activities have not been studied yet. Curcuma longa has been utilized as the main ingredients of traditional herbal medicines (jamu). The objective of this research was to investigate the antimicrobial and antioxidant activity of endophyte bacteria associated with Curcuma longa rhizome. Based on morphological characteristics of bacterial colonies, eight endophyte bacteria was isolated from Curcuma longa rhizome. Screening of endophyte isolate has antimicrobial activity was done using agar well diffusion method. The culture supernatant of each endophyte isolate was dropped on agar well against pathogenic bacteria Salmonella enterica ser. Typhi, Staphylococcus aureus and yeast Candida albicans. Three endophyte isolates K3, K2 and M1b showed antimicrobial activity against pathogenic bacteria and yeast. Isolate K3 showed strong antimicrobial activity against C. albicans and S. aureus, however, isolate K2 and isolate M1b showed antimicrobial activity against Salmonella enterica ser. Typhi and S. aureus, respectively. Those endophyte bacteria also had antioxidant activity shown by scavenging ability toward DPPH radical with consecutive percentage of isolate K3 (72.3 %), K2 (51.3 %) and M1b (64.6 %). Isolate K3 showed the highest antimicrobial and antioxidant activity. Based on biochemical characteristics using Microbact 24E kit, isolate K3 was identified as Paenibacilus alvei and isolate K2 as Enterobacter agglomerans. Keywords: antimicrobial, antioxidant, Curcuma longa's rhizome, endophyte bacteria.

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Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name Jehan Ramdani Hariyati

Contact Email jehanramdani@ub.ac.id

Phone +6282333752235

Journal Mail Official jehanramdani@ub.ac.id

Editorial Address Jl. Veteran Malang 65145

Location Kota malang, Jawa timur INDONESIA

Abstract

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Contact Name
Jehan Ramdani Hariyati

Contact Email
jehanramdani@ub.ac.id

Phone
+6282333752235

Journal Mail Official
jehanramdani@ub.ac.id

Editorial Address
Jl. Veteran Malang 65145

Location
Kota malang,

Jawa timur

INDONESIA