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INDONESIA
Health Science Journal of Indonesia
Published by Badan Penelitian dan Pengembangan Kesehatan, Kementerian Kesehatan
ISSN : 20877021     EISSN : 23383437     DOI : https://doi.org/10.22435/hsji
Core Subject : Health,
Dentistry Health Professions Nursing Public Health
Health Science Journal of Indonesia is a journal developed to disseminate and discuss the scientific literature and other research on the development of health. This journal is intended as a medium for communication among stake holders on health research such as researchers, educators, students, practitioners of Health Office, Department of Health, Public Health Service center, as well as the general public who have an interest in the matter. The journal is trying to meet the growing need to study health. Vision: Becoming a notable national journal in the field of health research and towards a reputable international journal. Mission: Providing scientific communication media in health research in order to advance science and technology in related fields. Organizes scholarly journal publishing in health research with an attempt to achieve a high impact factor in the development of science and technology.
Arjuna Subject : Kedokteran - Kesehatan Masyarakat, Kesehatan Lingkungan dan Pekerjaan
Articles 10 Documents
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Search results for , issue "Vol 12 No 2 (2021)" : 10 Documents clear
The importance of hospital re-accreditation: improving the timeliness of laboratory critical value reporting Wahyu Febrianto; Menis Rahmawati; I Gede Sastrawan; Tita Hariyanti
Health Science Journal of Indonesia Vol 12 No 2 (2021)
Publisher : Sekretariat Badan Penelitian dan Pengembangan Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/hsji.v12i2.3315

Abstract

Background: Patient safety is the main issue in healthcare services nowadays. Delaying to inform the critical value of laboratory results is a significant source of harm for the patient. The aim of this study is to compare the timeliness of laboratory critical value reporting before and after re-accreditation as one of the service quality indicators in Hospital X. Methods: This study was done by using observational cross-sectional in Hospital X on January - February 2020 with total sampling method of critical value reporting to the responsible clinician that originated from Intensive Care Unit (ICU), Verlos Kamer (VK), and inpatient ward (IW) 1-6 from January-December 2019. The timeliness of reporting was counted since the laboratory result was obtained until received by the responsible clinician within £ 30 minutes and categorized as "On time" or "Late". Results: During 2019, there were 816 reporting which has been done before re-accreditation (511) and after re-accreditation (305) with 17 kinds of tests. The most reported test was platelet with 349 (before re-accreditation) and 101 (after re-accreditation), whilst SGOT/SGPT and albumin were the fewest one. The lowest timeliness of reporting percentage was 76,00% (February), whilst the highest was 98,48% (November). The timeliness of reporting's percentage was 84,34% (before re-accreditation) and 94,43% (after re-accreditation). The statistical analysis result revealed Pearson Chi-Square correlation was 18,535 with significance 0,000 and 3,145 odds ratio which shows that re-accreditation could significantly increase the timeliness of critical value reporting three times. Conclusion: This result showed that re-accreditation could affect the timeliness of laboratory critical value reporting to the responsible clinicians. Keywords: re-accreditation, critical value, laboratory, patient safety, hospital Abstrak Latar belakang: Keselamatan pasien merupakan isu utama dalam pelayanan kesehatan. Tertundanya komunikasi hasil nilai kritis laboratorium merupakan sumber bahaya yang signifikan terhadap pasien. Penelitian ini bertujuan untuk membandingkan ketepatan waktu pelaporan nilai kritis laboratorium sebelum dan setelah reakreditasi sebagai salah satu indikator mutu di RS X. Metode: Penelitian dilakukan dengan cara observasional dengan metode cross sectional di RS X pada Januari - Februari 2020 dengan total sampling laporan nilai kritis kepada Dokter Penanggung Jawab Pasien (DPJP) yang berasal dari ruang Intensive Care Unit (ICU), Verlos Kamer (VK), dan ruang rawat inap 1 – 6 sejak Januari – Desember 2019. Ketepatan waktu pelaporan dihitung sejak hasil pemeriksaan didapatkan hingga diterima oleh DPJP dalam waktu £ 30 menit dan dinyatakan sebagai "Tepat Waktu" atau "Terlambat". Hasil: Selama tahun 2019, terdapat 816 pelaporan yang dilakukan sebelum akreditasi (511) dan setelahnya (305) dengan 17 jenis pemeriksaan. Pemeriksaan trombosit menjadi yang paling banyak dilaporkan yaitu 349 (sebelum akreditasi) dan 101 (setelah akreditasi), sedangkan SGOT/SGPT dan albumin menjadi yang paling sedikit. Persentase ketepatan waktu pelaporan paling rendah adalah 76,00% (Februari) sedangkan yang paling tinggi adalah 98,48% (November). Persentase ketepatan waktu pelaporan didapatkan 84,34% (sebelum akreditasi) dan 94,43% (setelah akreditasi). Hasil analisis statistik didapatkan korelasi Pearson Chi-Square 18,535 dengan signifikansi 0,000 dan Odds ratio 3,145 menunjukkan re-akreditasi mampu meningkatkan kemungkinan ketepatan waktu pelaporan nilai kritis sebesar tiga kali lipat. Kesimpulan: Hal ini menunjukkan bahwa re-akreditasi mampu mempengaruhi ketepatan waktu pelaporan nilai laboratorium kritis kepada DPJP. Kata kunci: re-akreditasi, nilai kritis, laboratorium, keselamatan pasien, rumah sakit.
Interferon gamma concentration in diabetes mellitus and dyslipidemia patient Nelly Marissa; Marlinda Marlinda; Maulidar Maulidar; Veny Wilya; Nur Ramadhan; Zain Hadifah
Health Science Journal of Indonesia Vol 12 No 2 (2021)
Publisher : Sekretariat Badan Penelitian dan Pengembangan Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/hsji.v12i2.4290

Abstract

Background: Patient with diabetes mellitus (DM) occurs chronic inflammation by characterized a decreased concentration of various cytokinin types. This causes changes in the body’s immunity so that can be easier in having an infection. One of the most important cytokines against infection is IFN-γ. This study aimed to determine IFN-γ concentration in DM and dyslipidemia patients. Metode: An amount of 234 people who received treatment at the health center in Banda Aceh in 2019 were included in this study. From each respondent, 5 ml of blood was taken to check fasting blood glucose, triglycerides, high-density lipoproteins (HDL), and interferon-gamma (IFN-γ). Test of fasting blood glucose, triglycerides, HDL was carried out using the colorimetric enzymatic method. The IFN-γ protein concentration was examined using the sandwich enzyme-linked immunosorbent assay (ELISA) technique. Result: IFN-γ concentration in the non-DM group was higher than in the DM group. There was a significant difference between the average IFN-γ concentration in the non-DM group compared with the DM group (p = 0.000). All DM patients had increased fasting blood glucose, most had hypertriglycerides, but HDL levels were normal. The fasting blood glucose group <126 mg / dl had a higher IFN-γ concentration than the group with fasting blood glucose levels ≥126 mg / dl. There was a significant difference in the concentration of IFN-γ between the two groups (p = 0.000). The group with triglyceride levels <150 mg / dl had lower IFN-γ levels than the group with triglyceride levels ≥ 150 mg / dl. There was a significant difference between the average IFN-γ concentration between those groups (p = 0.000). The fasting blood glucose levels ≥126 mg / dl and triglycerides levels ≥ 150 mg / dl had higher IFN-γ concentration than the group who had fasting blood glucose levels ≥126 mg / dl and triglycerides levels < 150 mg / dl. Conclusion: There are differences in IFN-γ concentrations in people with DM, increased fasting blood glucose and dyslipidemia compared to normal people. Keywords: IFN-γ, diabetes mellitus, dyslipidemia Abstrak Latar belakang: Penderita diabetes mellitus (DM) dan dyslipidemia mengalami inflamasi kronik yang ditandai dengan perubahan konsentrasi berbagai sitokin. Hal ini yang menyebabkan perubahan imunitas tubuh sehingga mudah mengalami infeksi. Salah satu sitokin yang paling berperan terhadap infeksi adalah interferon gamma (IFN-γ). Penelitian ini bertujuan untuk memeriksa konsentrasi IFN-γ pada penderita DM dan dislipidemia. Metode: Sebanyak 234 orang yang melakukan pengobatan di puskesmas di Kota Banda Aceh pada tahun 2019 diikutsertakan dalam penelitian ini. Dari setiap responden dilakukan pengambilan darah sebanyak 5 ml untuk dilakukan pemeriksaan kadar gula darah puasa (KGD P), trigliserida, high density lipoprotein (HDL), dan inrferon- gamma (IFN-γ). Pemeriksaan KGD, trigliserida, HDL dilakukan dengan metode enzimatik kolorimetrik. Pemeriksaan konsentrasi protein IFN-γ menggunakan teknik sandwich Enzyme-linked immunosorbent assay (ELISA). Hasil: Konsentrasi IFN-γ pada kelompok non-DM lebih tinggi dibandingkan dengan kelompok DM. Terdapat perbedaan bermakna antara rata-rata konsentrasi IFN-γ pada kelompok non-DM dibandingkan dengan kelompok DM (p=0,000). Semua penderita DM mengalami peningkatan KGD P, sebagian besar mengalami hipertrigliserida, namun kadar HDL normal. Pada kelompok KGD P <126 mg/dl memiliki konsentrasi IFN-γ yang lebih tinggi dibandingkan dengan kelompok dengan KGD P ≥126 mg/dl. Terdapat perbedaan bermakna perbedaan konsentrasi IFN-γ antar kedua kelompok tersebut (p=0,000). Kelompok dengan kadar trigliserida <150 mg/dl memiliki kadar IFN-γ lebih rendah dibandingkan dengan kelompok dengan kadar trigliserida ≥ 150 mg/dl. Terdapat perbedaan bermakna antara rata-rata konsentrasi konsentrasi IFN-γ antar kedua kelompok tersebut (p=0,000). Pada kelompok KGD P ≥126 mg/dl dan trigliserida ≥ 150 mg/dl memiliki kadar IFN-γ yang lebih tinggi dibandingkan dengan kelompok KGD P ≥126 mg/dl namun trigliserida <150 mg/dl. Terdapat perbedaan bermakna antara rata-rata konsentrasi konsentrasi IFN-γ antar kedua kelompok tersebut (p=0,000). Kesimpulan: Terdapat perbedaan konsentrasi IFN-γ pada orang dengan DM, peningkatan KGD P dan dislipidemia dibandingkan dengan orang normal. Kata kunci : IFN-γ, diabetes mellitus, dislipidemia.
The relationship of smoking duration, sleep disorders, and nutritional status of Indonesian adult men: data analysis of the 2014 Indonesian Family Life Surve Relawantria Harlianti; Trias Mahmudiono; Dominikus Raditya Atmaka; Siti Helmyati; Mira Dewi; Cindra Tri Yuniar
Health Science Journal of Indonesia Vol 12 No 2 (2021)
Publisher : Sekretariat Badan Penelitian dan Pengembangan Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/hsji.v12i2.5243

Abstract

Background: In Indonesia, the prevalence of smoking is increasing from year to year and can cause various health problems, such as sleep disorders and affect a person's nutritional status. So, in this study, the relationship between smoking duration, sleep disturbances, and nutritional status in men aged 26–45 years will be investigated using secondary data from the 2014 Indonesia Family Live Survey (IFLS). Methods: The 2014 secondary data from the fifth waves of the IFLS were used for analysis. All 5,379 data of men aged 26–45 years who provided anthropometric, smoking duration, and sleep disorders were included in the study. The Chi-Square test was used to examine the relationship between smoking duration, sleep disorders, and nutritional status in men aged 26 – 45 years. Furthermore, the Multinomial Logistics Regression test is carried out to determine the variables that have the strongest influence. Results: Based on the results of statistical tests conducted, it was found that the majority of respondents had a smoking duration of 11-20 years, of which 27.2% of respondents did not experience sleep disorders and 25.4% had sleep disorders. The nutritional status of respondents with a smoking duration of 11-20 years is normal as many as 35% of respondents and at least 0.5% of respondents have underweight nutritional status with smoking duration <5 years. Furthermore, the test results of the relationship between smoking duration and sleep disturbances obtained p-value = 0.03 and the relationship between smoking duration and nutritional status obtained p-value <0.01. Conclusion: Smoking duration was associated with sleep disorder and overweight nutritional status in men aged 26 – 45 years. Keywords: smoking duration, sleep disorder, nutritional status, tobacco use, sleeping sickness Abstrak Latar belakang: Di Indonesia, prevalensi merokok semakin meningkat dari tahun ke tahun dan dapat menyebabkan berbagai permasalahan kesehatan, seperti gangguan tidur serta mempengaruhi status gizi seseorang. Sehingga pada penelitian ini akan diteliti hubungan antara durasi merokok, gangguan tidur, dan status gizi pada pria berusia 26–45 tahun menggunakan data sekunder dari Indonesia Family Live Survey (IFLS) tahun 2014. Metode: Analisis dari data sekunder gelombang kelima IFLS tahun 2014. Semua 5.379 data pria berusia 26–45 tahun yang memiliki kelengkapan data antropometri, kebiasaan merokok, dan gangguan tidur diikutkan dalam penelitian. Uji Chi-Square digunakan untuk menguji hubungan antara durasi merokok, gangguan tidur, dan status gizi pada pria berusia 26 – 45 tahun. Selanjutnya uji Regresi Logistik Multinomial dilakukan untuk mengetahui variabel yang memiliki pengaruh paling kuat. Hasil: Berdasarkan hasil dari uji statistik yang dilakukan, didapatkan bahwa mayoritas responden memiliki durasi merokok selama 11-20 tahun, dimana sebanyak 27.2% responden tidak mengalami gangguan tidur dan 25.4% mengalami gangguan tidur. Status gizi paling banyak yang dimiliki oleh responden dengan durasi merokok selama 11-20 tahun adalah normal sebanyak 35% responden dan yang paling sedikit sebanyak 0.5% responden memiliki status gizi underweight dengan durasi merokok <5 tahun. Selanjutnya hasil uji hubungan antara durasi merokok dengan gangguan tidur didapatkan nilai p-value=0.03 dan hubungan antara durasi merokok dengan status gizi didapatkan nilai p-value<0.01. Kesimpulan: Durasi merokok berhubungan dengan gangguan tidur dan status gizi overweight pada laki-laki usia 26 – 45 tahun. Kata kunci: durasi merokok, gangguan tidur, status gizi, penggunaan tembakau, penyakit tidur
Priority setting in responding crisis: a hospital leaders’ perspective at the early stage of COVID-19 pandemic Aryo Dewanto; Yudi Setyawan; Viera Wardhani
Health Science Journal of Indonesia Vol 12 No 2 (2021)
Publisher : Sekretariat Badan Penelitian dan Pengembangan Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/hsji.v12i2.5295

Abstract

Background: The COVID-19 pandemic hit Indonesia when hospitals were striving to adjust to a changing environment after a new health insurance system implementation, a government’s effort to achieve Universal Health Coverage. As a result, the pandemic forced hospitals to exploit their resources. Due to limited resources, setting accurate priorities is highly important to secure hospital operations and maintain its track towards the expected goals. This study aims to explore how deep the crisis impacts hospitals and how hospital leaders in Indonesia set their priorities in responding to the impact of this pandemic. Methods: This study used a descriptive and analytical approach. Data were collected through an online survey from hospital leaders and several documentary sources. Results: The results show that almost all hospital directors consider patient visits and hospital finance the most significant impacts of the COVID-19 pandemic. However, government hospital directors emphasize different areas compared to non-government hospital directors; the former sets their priorities on the hospital’s human resources, quality of service, and operations, while the latter focuses on the impact of patient visits and hospital finance. Conclusion: Although directors of government and non-governmental hospitals have a different emphasis, their priority is the same, maintaining hospital sustainability to provide quality services to people. Keywords: COVID-19 pandemic, hospital leaders’ perspective, impacts, Indonesia, priority setting Abstrak Latar belakang: Pandemi COVID-19 melanda Indonesia ketika rumah sakit berusaha menyesuaikan diri dengan lingkungan yang berubah setelah penerapan sistem jaminan kesehatan baru sebagai upaya pemerintah untuk mencapai Universal Health Coverage. Akibatnya, pandemi memaksa rumah sakit untuk mengeksploitasi sumber daya mereka. Sumber daya yang terbatas membuat penetapan prioritas yang akurat menjadi sangat penting untuk menjamin keberlangsungan operasional rumah sakit dan memastikan rumah sakit bergerak menuju tujuan yang diharapkan. Penelitian ini bertujuan untuk mengeksplorasi seberapa dalam dampak krisis ini terhadap rumah sakit dan bagaimana pemimpin rumah sakit di Indonesia menetapkan prioritasnya dalam merespon dampak pandemi ini. Metode: Penelitian ini menggunakan pendekatan deskriptif dan analitik. Data dikumpulkan melalui survei online dari pimpinan rumah sakit dan beberapa sumber dokumenter. Hasil: Hasil penelitian menunjukkan bahwa hampir semua direktur rumah sakit menganggap kunjungan pasien dan pembiayaan rumah sakit mendapat dampak paling signifikan dari pandemi COVID-19. Namun, direktur rumah sakit pemerintah menekankan bidang yang berbeda dibandingkan dengan direktur rumah sakit non-pemerintah. Direktur rumah sakit pemerintah menetapkan prioritas mereka pada sumber daya manusia rumah sakit, kualitas layanan, dan operasi, sedangkan direktur rumah sakit non-pemerintah fokus pada dampak kunjungan pasien dan keuangan rumah sakit. Kesimpulan: Meskipun direktur rumah sakit pemerintah dan non-pemerintah memiliki penekanan yang berbeda, tetapi prioritas mereka sama yaitu menjaga keberlanjutan rumah sakit untuk memberikan pelayanan yang berkualitas kepada masyarakat. Kata kunci: pandemi COVID-19, perspektif pemimpin rumah sakit, dampak, Indonesia, penetapan prioritas.
Factors associated with measles antibody titers in children aged 12-36 months in Indonesia: an analysis of National Health Research 2013 Ni Ketut Aryastami; Prisca Petty Arfines; Vivi Setiawaty; Siti Isfandari
Health Science Journal of Indonesia Vol 12 No 2 (2021)
Publisher : Sekretariat Badan Penelitian dan Pengembangan Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/hsji.v12i2.5356

Abstract

Background: The immunization program in Indonesia has been implemented since 1956 started to eradicate smallpox and expanded until 1980, including Measles. The timely and complete implementation of basic immunization is the main strategy to protect the population, including outbreak prevention. The purpose of this study is to determine the level immunity of Measles antibody as the outcome of completed basic immunization and its contributors in children aged 12-36 months. Methods: This study is a secondary data analysis of the Indonesia Basic Health Survey (RISKESDAS) 2013. The analysis was carried out on a serological sample of the antibody titer of children aged 12-36 months, totaling 229 samples. The sample inclusion criteria were children who had complete sociodemographics data, basic immunization records and Measles antibody titer data. Measles examination was carried out using the Enzyme-Linked Immunosorbent Assay (ELISA) method. Results: Incomplete immunization, being a boy, and lack of cleanliness in the family room were significantly associated with lower measles antibody levels in children. Having each variable controlled, completeness of immunization (OR=1,99; p=0.018; 95% CI=1.124-3.544) and gender of boy (OR=2.0; p=0.016; 95% CI=1.137-3.515) remain as significant variables for antibody’s titer. Conclusion: The completeness of immunization has a significant association towards titer antibody of Measles in children. Immunization completeness is an actual effort to reach herd immunity in children and to prevent measles outbreak in the community. Adequate health promotion is needed to change people's behavior to believe in the safety and importance of implementing complete basic immunization for children even in pandemic conditions. Keywords: antibody titer, immunization, children aged 12-36 months, Indonesia, measles Abstrak Latar belakang: Program imunisasi di Indonesia telah dilaksanakan sejak tahun 1956 yang dimulai dengan pemberantasan cacar yang diperluas hingga tahun 1980, termasuk campak. Pelaksanaan imunisasi dasar yang tepat waktu dan lengkap merupakan strategi utama untuk perlindungan penduduk, termasuk pencegahan Kejadian Luar Biasa (KLB). Tujuan dari penelitian ini untuk mengetahui tingkatan kekebalan antibodi Campak sebagai hasil dari kelengkapan imunisasi dasar dan faktor yang berkontribusi pada anak usia 12-36 bulan. Metode: Penelitian ini merupakan analisis data sekunder Riset Kesehatan Dasar Indonesia (RISKESDAS) 2013. Analisis dilakukan pada sampel serologi titer antibodi anak usia 12-36 bulan yang berjumlah 229 sampel. Kriteria inklusi sampel adalah anak yang memiliki data sosiodemografi lengkap, catatan imunisasi dasar dan data titer antibodi Campak. Pemeriksaan campak dilakukan dengan metode Enzyme-Linked Immunosorbent Assay (ELISA). Hasil: Imunisasi yang tidak lengkap, berjenis kelamin laki-laki, dan kurangnya kebersihan di ruang keluarga berhubungan bermakna dengan rendahnya tingkat antibodi campak pada anak. Setelah masing-masing variabel terkontrol, kelengkapan imunisasi (OR=1,99; p=0,018; 95% CI=1.124-3.544) dan jenis kelamin laki-laki (OR=2.0; p=0.016; 95% CI=1.137-3.515) merupakan variabel yang tetap berhubungan dengan titer antibodi secara signifikan. Kesimpulan: Kelengkapan imunisasi memiliki hubungan yang bermakna terhadap titer antibodi Campak pada anak. Kelengkapan imunisasi merupakan upaya nyata untuk mencapai herd immunity pada anak dan mencegah wabah campak di masyarakat. Promosi kesehatan yang memadai diperlukan untuk mengubah perilaku masyarakat agar percaya akan keamanan dan pentingnya pelaksanaan imunisasi dasar lengkap bagi anak meskipun dalam kondisi pandemi. Kata kunci: titer antibodi, imunisasi, anak usia 12-36 bulan, Indonesia, campak
Spatial variation of tuberculosis risk in Indonesia 2010-2019 Tities Puspita; Anton Suryatma; Oster Suriani Simarmata; Ginoga Veridona; Heny Lestary; Athena Athena; Imran Pambudi; Sulistyo Sulistyo; Tiffany Tiara Pakasi
Health Science Journal of Indonesia Vol 12 No 2 (2021)
Publisher : Sekretariat Badan Penelitian dan Pengembangan Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/hsji.v12i2.5467

Abstract

Background: As the second-highest country in tuberculosis (TB) cases globally, Indonesia has experienced an increasing trend of notification rate in the last ten years; however, the 34 provinces may have different risks. This study aims to examine TB risk variation across Indonesia in 2010-2019. Methods: A descriptive analysis was conducted on TB routine data of 2010-2019 from the Ministry of Health. Cases included all types of TB patients. Total cases, incidence rate (IR), and standardized morbidity ratio (SMR) were calculated for each province and national level during the period. Distributions of IRs and SMRs were displayed on maps. Results: During 2010-2019, 3,866,447 TB cases occurred in Indonesia, and the national IR was 1,523 per 100,000 populations. The highest proportion of cases and IR were in West Java (20.6%, 314 per 100,000); while the lowest was in North Kalimantan (0.2%, 3 per 100,000). Higher risks of TB occurred in DKI Jakarta (SMR 1.9), Papua (1.7), North Sulawesi (1.7), Maluku (1.5) and West Papua (1.5) among others. The smallest SMRs were found in Bali and Yogyakarta (0.5). Conclusion: TB risk varied across Indonesia in 2010-2019, with a higher risk in DKI Jakarta and several provinces in eastern Indonesia. Given the underreporting nature of routine data, validation is required when using the finding of this study in the local-level intervention. Keywords: tuberculosis, TB, standardized morbidity ratio, spatial variation, risk Abstrak Latar belakang: Sebagai negara dengan jumlah kasus tuberkulosis (TB) terbesar kedua di dunia, Indonesia menunjukkan tren peningkatan notification rate di sepuluh tahun terakhir. Akan tetapi, risiko TB di 34 provinsi bisa saja berbeda-beda. Artikel ini bertujuan mengkaji variasi risiko TB di Indonesia pada tahun 2010-2019. Metode: Data rutin TB tahun 2010-2019 dari Kementerian Kesehatan dianalisis secara deskriptif. Kasus TB didefinisikan sebagai semua tipe pasien TB. Total jumlah kasus, incidence rate (IR), dan standardized morbidity ratio (SMR) dihitung untuk tiap provinsi dan tingkat nasional selama periode tersebut. Sebaran IR dan SMR diplot di atas peta. Hasil: Selama 2010-2019, terdapat 3.866.447 kasus TB dan IR nasional 1.523 per 100.000 populasi. Proporsi kasus dan IR terbesar ada di Jawa Barat (20,6%, 314 per 100.000) dan terkecil di Kalimantan Utara (0,2%, 3 per 100.000). Risiko TB lebih tinggi di antaranya terjadi di DKI Jakarta (SMR 1,9), Papua (1,7), Sulawesi Utara (1,7), Maluku (1,5) dan Papua Barat (1,5). Standardized Morbidity Ratio terendah ditemukan di Bali dan Yogyakarta (0,5). Kesimpulan: Dapat disimpulkan bahwa risiko TB beragam di seluruh Indonesia selama 2010-2019, di mana DKI Jakarta dan beberapa provinsi di timur Indonesia memiliki risiko lebih tinggi. Mengingat adanya kurang lapor dalam data rutin, validasi diperlukan jika menggunakan temuan studi ini dalam intervensi di tingkat lokal. Kata kunci: tuberkulosis, TB, standardized morbidity ratio, variasi spasial, risiko
Optimization of multiplex real-time RT-PCR for respiratory syncytial viruses detection Agustiningsih Agustiningsih
Health Science Journal of Indonesia Vol 12 No 2 (2021)
Publisher : Sekretariat Badan Penelitian dan Pengembangan Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/hsji.v12i2.5529

Abstract

Abstract Background: Multiplex real-time RT-PCR (rRT-PCR) is a fast, sensitive and specific test to detect more than one target in single PCR reaction. In this study we developed multiplex rRT-PCR for RSV-A and RSV-B since those viruses are the most common pathogen found in respiratory tract. However, in order to gain optimal reaction for RSV-A and RSV-B detection, the optimization of primers and probes specific for RSV-A and RSV B are needed. Method: The primers and probes of multiplex rRT-PCR for RSV-A and RSV-B were selected and optimized utilizing PerlPrimer software and BLAST to analyze the secondary structures and specificity, respectively. Further testing of selected primers and probes for rRT-PCR was done using annealing temperature based on in silico analysis as mentioned above. This includes sensitivity testing with the utilization of synthesized DNA of RSV-A and RSV-B and specificity testing targeting the common viruses found in respiratory tract. Results: The primer set and probes selected for RSV-A and RSV-B detection were specific only for RSV-A and RSV-B and showed no secondary structure. Based on primer and probe criteria for rRT-PCR such as annealing temperature, no secondary structure formed, % GC content and limit of detection, the multiplex rRT-PCR test using selected primers and probes was able to detect synthesized DNA of RSV-A and RSV-B. Conclusion: Multiplex rRT-PCR that employing primer sets and probes targeted N gene of RSV-A and RSV-B in this study were able to be detect RSV-A and RSV-B in single PCR reaction. Keyword: Multiplex, real-time RT-PCR, RSV-A, RSV-B Abstrak Latar belakang: Multiplex real-time RT-PCR (rRT-PCR) merupakan metode yang cepat, sensitif dan spesifik untuk mendeteksi lebih dari satu target pathogen dalam satu reaksi PCR. Penelitian ini bertujuan untuk mengembangkan multiplex rRT-PCR virus RSV-A dan RSV-B yang merupakan patogen yang paling sering ditemukan di saluran pernafasan. Optimisasi dari primer dan probe dalam multiplex rRT-PCR diperlukan untuk mendapatkan reaksi yang optimal dalam deteksi virus RSV-A dan RSV-B. Metode: Primer dan probe untuk multiplex rRT-PCR RSV-A dan RSV-B dipilih dan dioptimasi menggunakan software PerlPrimer dan BLAST untuk menganalisis adanya struktur sekunder serta spesifisitas dari primer dan probe. Uji multiplex rRT-PCR dilanjutkan berdasarkan suhu annealing berdasarkan hasil analisis menggunakan PerlPrimer. Uji sensitifitas dilakukan dengan menggunakan DNA sintetis dari RSV-A dan RSV-B dan uji spesifisitas dilakukan dengan mengetes primer dan probe terhadap virus-virus lain yang umumnya ditemukan di saluran pernafasan. Hasil: Primer dan probe yang dikembangkan pada penelitian ini tidak membentuk struktur sekunder dan spesifik mengamplifikasi hanya RSV-A dan RSV-B. Berdasarkan kriteria primer dan probe untuk digunakan dalam rRT-PCR yaitu suhu annealing, tidak adanya pembentukan struktur sekunder, % GC content serta detection limit, uji multiplex rRT-PCR yang dikembangkan pada penelitian ini mampu mendeteksi DNA sintetis RSV-A dan RSV-B. Kesimpulan: Multiplex rRT-PCR dengan menggunakan primer dan probe untuk RSV-A dan RSV-B dapat mendeteksi RSV-A dan RSV-B dalam satu reaksi PCR. Kata kunci: multiplex, real-time RT-PCR, RSV-A, RSV-B
Intake of kidney bean (phaseolus vulgaris) extract as postpartum blues management Desta Ayu Cahya Rosyida; Nina Nikmah; Khoiriyah Noviastuti
Health Science Journal of Indonesia Vol 12 No 2 (2021)
Publisher : Sekretariat Badan Penelitian dan Pengembangan Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/hsji.v12i2.4938

Abstract

Background: Postpartum blues is common in postpartum Mother with more prone to crying, more anxious, often unstable and more emotional than usual. Zinc supplementation is one of the methods needed for postpartum blues conditions. Kidney bean (phaseolus vulgaris) extract has the highest zinc content. This study aimed to examine the effect of intake kidney bean extract (phaseolus vulgaris) as a zinc source as management mother with Postpartum Blues Methods: Quantitative study with an experimental study design (Quasi Experiment Design) with a Pre and Post-test Control Group Design. The instrument used to measure the mood of a mother after childbirth was EPDS (Edinburgh Post-Partum Depression Scale). The total sample was 68 mothers who experienced postpartum blues in Ngerandu Ponorogo primary health care. The Mann-Whitney was used to analyse and find out the difference in mood improvement before and after the intervention in the experimental group and the control group. Results: The results showed that there was a statistically significant difference in mood levels of mothers with postpartum blues before and after intervention (p value of 0.001 (<0.005). This result indicated that intake of kidney bean (Phaseolus vulgaris) extract had an effect on the mood level of mothers with postpartum blue. Conclusion: Intake of kidney bean (phaseolus vulgaris) extract on the incidence of postpartum blues possibly improvement mood in the experimental group after the treatment for 1 month. Keywords: kidney bean (phaseolus vulgaris) extract, postpartum blues, postpartum mother Abstrak Latar belakang: Postpartum blues umum terjadi pada ibu postpartum dengan tanda sering menangis, khawatir yang berlebihan, emosional yang tidak stabil. Suplementasi zinc merupakan salah satu metode yang dibutuhkan untuk kondisi postpartum blues. Ektrak kacang merah (phaseolus vulgaris) memiliki sumber nutrisi zinc yang tinggi, Penelitian ini bertujuan untuk menguji efek konsumsi ekstrak kacang merah (phaseolus vulgaris) sebagai sumber zinc sebagai manajemen ibu dengan postpartum blues Metode: Penelitian kuantitatif dengan design Quasi Experiment pre dan post control grup. Instrumen pengukur mood ibu setelah melahirkan adalah EPDS (Edinburgh Post-Partum Depression Scale). Total Sampel 68 ibu yang mengalami postpartum blues di Puskesmas Ngerandu Ponorogo. Analisis statistik menggunakan Mann-Whitney untuk menemukan perbedaan perubahan mood ibu postpartum blues sebelum dan sesudah dilakukan intervensi pada grup intervensi dan kontrol. Hasil: Hasil menunjukkan bahwa terdapat perbedaan signifikan tingkat mood ibu dengan postpartum blues sebelum dan sesudah dilakukan intervensi (p : 0,001 (<0,005). Hasil mengindikasikan bahwa konsumsi ekstrak kacang merah (phaseolus vulgaris) memiliki efek pada tingkat mood ibu yang sedang mengalami postpartum blues. Kesimpulan: Konsumsi ekstrak kacang merah (phaseolus vulgaris) pada ibu postpartum blues memungkinkan untuk perubahan mood pada grup intervensi setelah diberikan treatment selama 1 bulan. Kata kunci: ekstrak kacang merah (phaseolus vulgaris), postpartum blues, ibu postpartum
Front Matter HSJI Volume 12 no.2 2021 hsji author
Health Science Journal of Indonesia Vol 12 No 2 (2021)
Publisher : Sekretariat Badan Penelitian dan Pengembangan Kesehatan

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Abstract

Front Matter HSJI Volume 12 no.2 2021
Back Matter HSJI Volume 12 no.2 2021 hsji author
Health Science Journal of Indonesia Vol 12 No 2 (2021)
Publisher : Sekretariat Badan Penelitian dan Pengembangan Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Back Matter HSJI Volume 12 no.2 2021

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