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Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Core Subject : Science,
Journal of the Medical Sciences (JMedSci) or Berkala Ilmu Kedokteran (BIK) is an international, open-access, and double-blind peer-reviewed journal, published by Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada Yogyakarta Indonesia. JMedSci aiming to communicate high-quality articles in the areas of biomedical science from basic to clinical sciences.The journal welcomes papers from original articles, case reports, reviews, and book reviews. All papers published in JMedSci are freely available as downloadable pdf files. The journal began its publication on March 1973 and published quarterly (January, April, July, and October). JMedSci is abstracted and indexed in DOAJ, Crossref, Google Scholar, Sinta, Indonesia One Search. JMedSci is accredited by Directorate of General Higher Education, the Ministry of Research, Technology, and Higher Education, Indonesia
Articles 2,170 Documents
The influence of Pfannenstiel and midline incisions to the wound condition on the third day post gynecologic surgery Ibnu Pranoto, Nita Tri Kurniati Sulchan Sofoewan
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 44, No 02 (2012)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (340.862 KB)

Abstract

Many factors should be considered by a surgeon to choose an abdominal incision, such as incision extensibility, speed of operation, presence or absence of adhisions, hemostasis problems, possibility of postoperative complications such as infections, hernia or dehiscence, and wound healing. Midline or linea mediana incision makes the field operation seem wider, therefore the operations can be performed more uickly. Meanwhile, transverse incision such as Pfannenstielincision is gaining popularity with the less incidence of infection, hernia, and wound dehiscence because this technique follows Langer’s lines resulting in reduced traction at the skin edges and making better wound closure and approximation. This study aimed to evaluate the influence ofPfannenstiel and midline incisions to the wound condition on the third day post gynecologic surgery. This was an observational study using a prospective cohort study design. The subjects were patients with the indications for gynecologic surgery in Dr. Sardjito General HospitalYogyakarta and affiliated hospitals who fulfilled the inclusion and exclusion criteria. Sixty subjects were divided into two groups i.e. 30 subjects who underwent Pfannenstiel incision as treatment group and 30 subjects who underwent linea mediana incision as control group. The characteristicsof subjects of both group were not significantly different. No significant difference of the wound condition on the third day after surgery between subjects who underwent Pfannenstiel and linea mediana incisions was observed in this study (p=0.212). The risk of the wound beingnot dried yet on the third day after sugery on subjects who underwent linea mediana incision technique was 2.5 times higher compared to subjects who underwent Pfannenstiel incision technique. However, it was not statistically different (p= 0.212; RR= 2.5; 95%CI= 0.525-11.894). In conclusion, there is no significantly different effect of Pfannenstiel and linea mediana incisions to the wound condition on the third day post gynecologic surgery.Keywords: Pfannenstiel incision - midline incision - wound healing - gynecologic surgery -complication
Parapagus dicephalus conjoined twins and evaluation of ischiopagus tetrapus conjoined twins in Indonesia: a case report ., ROCHADI
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 45, No 03 (2013)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1263.444 KB) | DOI: 10.19106/JMedScie004503201306

Abstract

Conjoined twins are rare occurrences in medical practice. In this study, two cases of conjoinedtwins, parapagus dicephalus and ischiopagus tetrapus are reported in Dr. Sardjito General Hospital,Yogyakarta, Indonesia. The first case i.e. five-day-old male parapagus dicephalus conjoinedtwins referred to the hospital. The twins have two heads, two arms and two legs. X-ray examinationreveals two vertebrae collumn, single heart, and single pelvis. Moreover ultrasonographyexamination reveals conjoining of liver and echocardiography examinatin reveals single atrium.The twins were diagnosed as parapagus dicephalus. As a result of their fusion, operative carehad been considered to be unacceptable. After several weeks in neonatal intensive care, theydied. The second case i.e. two-day-old female conjoined twins with conjoining at ischium regionreferred to the hospital. Ultrasonography and abdominal examination reveal conjoining atintestinum, buttock and ischium. The twins was diagnsed as ischiopagus tetrapus. They weresuccessfully separated at 6 months of age. They are alive and well so far for more than 7 years.In conclusion, the prognosis of conjoined twins is related to the type, extent of union and organabnormalities. The successful separation of conjoined twins might be determined by good planningand staff enthusiasm and dedication.
Prognostic predictor at Pediatrics Intensive Care Unit (PICU) with Pediatric Risk of Mortality III (PRISM III) scores Roni Naning, Vita Susianawati Purnomo Suryantoro
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 46, No 02 (2014)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (319.94 KB) | DOI: 10.19106/JMedScie004602201403

Abstract

Various mortality prognostic scoring system are available for predicting mortality risk in PediatricIntensive Care Unit (PICU). The Pediatric Risk of Mortality III (PRISM III) scoring system is one ofthe main indicators used in the PICU. This study was conducted to evaluate the PRISM III asprognostic predictor in PICU. This was a cohort study involving 64 patients who admitted toPICU in Dr. Sardjito General Hospital, Yogyakarta and met inclusion and exclusion criteria. Theclinical state of patients were assessed and the PRISM III scores corresponding to the firs 24hours of hospitalization were calculated. Outcome analysis was defined either as death ordischarged from the hospital were recorded. Multivariate analysis was performed to find outindependent predictive factor that influence the outcome of death. The discriminative power ofthe model was calculated based on the receiver operator curve (ROC). The result showed thatmental status (relative risk/RR: 13.21; 95%CI: 1.18-14.80), White Blood Count/WBC (RR: 19.51;95%CI: 18.12-25.15) and Blood Urea Nitrogen/BUN (RR:22.87; 95%CI:1.85-28.20) were foundto be the main predictive factors of death in PICU. The cut off value of 51 of PRISM III scoreyielded the best sensitivity (83%) and specificity (69%). In conclusion, PRISM III score can beused as a prognostic predictor to determine the death risk of patients hospitalized at PICU.
Anti-hepatitis B surface (anti-HBs) seroprotection in children with acute lymphoblastic leukemia post hepatitis B vaccination in Indonesia Roni Naning, Yustinah Nenny Sri Mulyani
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 46, No 01 (2014)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (299.908 KB) | DOI: 10.19106/JMedScie004601201402

Abstract

Children with acute lymphoblastic leukemia (ALL) receiving chemotherapy are at high risk fordeveloping hepatitis B infection. Protective effect of hepatitis B vaccination in children withALL in Indonesia has not been investigated yet. The aim of study was to determine the proportiondifference of anti-HBs seroprotection between children with ALL and without malignancy posthepatitis B vaccination. We had conducted a case-control study from January to February 2012at Dr. Sardjito General Hospital, Yogyakarta. We ascertained 1-15 years old children with ALLreceiving chemotherapy and without malignancy who had hepatitis B vaccination. Sixty sevenchildren were included in this study. Anti-HBs seroprotection level was measured using anenzyme-linked immunosorbent assay (ELISA). The results were analyzed using Chi-square test.Twenty one children (65.6%) with ALL and 13 children (37.1%) without malignancy showedanti-HBs seroprotection. The difference was statistically significant with p-value of 0.020(prevalence ratio [PR]=0.3; 95% CI=0.11-0.84). Both groups showed no significantly differenceof anti-HBs seroprotection according to either female gender, risk classification, or phase ofchemotherapy with p-value of 0.38 (PR =4; 95% CI=1.05-15.2), 0.248 (PR =3.37; 95%CI=0.58-19.6) or 0.214, respectively. In conclusion, the proportion of anti-HBs seroprotectionin children with ALL is higher than those without malignancy.
The influence of iron supplementation in pregnant women to the occurrence of low birth weight (LBW) babies in Palu, Central Sulawesi R. Detty Siti Nurdiati Z, Sri Restu Djaswadi Dasuki
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 46, No 01 (2014)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (464.054 KB) | DOI: 10.19106/JMedScie004601201406

Abstract

Low birth weight (LBW) babies remains a major problem world wide as it causes a high rate ofneonatal morbidity and mortality. One effort to reduce the prevalence of LBW babies is byproviding iron supplementation to the pregnant women. The aim of this study was to examinethe relationship between iron supplementation program and prevalence of LBW babies in Palu,Central Sulawesi. This was an observational study with case control design. Subjects were allLBW babies from mothers that had accepted iron supplementation during pregnancy. Samplesize in this study was 87 for case and 87 for control. Bivariate analysis showed that ironsupplementation that not comply the program had a significant correlation with prevalence ofLBW babies (p=0.01). Other significant factors include abnormal hemoglobin level (p=0.01),body mass index (BMI) (p=0.02), educational level (p=0.02), multiparity (p=0.03), andgestational age (prematurity) (p=0.03). Multivariate analysis revealed that the strongest riskfactor for the occurrence of LBW babies was iron supplementation that not comply the program(OR= 3.82; 95% CI: 1.77- 8.22). Other risk factors were hemoglobin level (OR= 3.45; 95% CI:1.59-7.49), BMI (OR= 2.27; 95% CI: 1.05-4.91), gestational age (OR= 3.11; 95% CI: 1.45-6.67), multiparity (OR= 2.98, 95% CI: 1.36-6.51), and educational level (OR= 2.38, 95% CI:1.12-5.03). Based on the analysis, the strongest risk factors that affected the prevalence of LBWwas iron supplementation, abnormal hemoglobin level, gestational age, multiparity and educationlevel. In conclusion, iron supplementation during pregnancy that not comply with the programwas the strongest risk factor of LBW babies. The prevalence of LBW babies can be reduced bycontrolling of iron supplementation, hemoglobin level, BMI, gestational age, parity and education.
Relationship between perimenopause symptoms and bone mineral density Siswishanto Rukmono, Aida Djaswadi Dasuki
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 46, No 01 (2014)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (334.833 KB) | DOI: 10.19106/JMedScie004601201404

Abstract

The menopausal transition or perimenopause is associated with numerous symptoms. Womenentering perimonopause normally have menstrual irregular periods due to an imbalance of serumestradiol. Since the status of estrogen is known as an important determinant of bone massdensity, menopausal symptoms can be an effective indicator of bone status in women. Thisstudy aimed to evaluate the relationship between perimenopause symptoms and bone mineraldensity (BMD). This was an observational study using cross-sectional design conducted duringthree months periode from June to August 2010 in Kalangan Village, Banguntapan, BantulDistrict. Subjects were women between the aged 46-55 years who fulfilled the inclusion andexclusion criteria. After underwent history taking, subjects underwent physical examinationaccording to International Physical Activity Questionnaires (IPAQ). The BMD of subjects wasthan measured using dual-energy X-ray absorptiometry (DEXA). The relationship betweenperimenopause symptoms and BMD was then evaluated using multivariate analysis. The resultsshowed that perimenopause symptoms increase abnormal BMD of subjects 1.38 times higherthan those without perimenopause symptoms (RR=1.38; 95%CI 1.39-13.78; p=0.01). Inconclusion, the perimenopause symptoms is associated with the high proportion of abnormalBMD in women.
Cytochrome P450 aromatase (CYP19) gene expression in ovarian granulose cells of hypothyroid rats induced by propylthiouracil Totok Utoro, Prihatin Broto Sukandar Sri Kadarsih Soejono
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 45, No 03 (2013)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1950.425 KB) | DOI: 10.19106/JMedScie004503201302

Abstract

Thyroid hormones are proven to have a direct effect on granulose cells, luteal cells and oocytesdue to their role in gonadotropin action on steroid hormone production. In vitro study showedthat tiroxine (T4) on granulose cells can stimulate ovarian steroidogenesis. Moreover, highconcentration of triiodothyronine (T3) increases the estradiol secretion and aromatase mRNAexpression. Hypothyroidism influences the cytochrome P450 aromatase (CYP19) gene expression.The aim of this study was to evaluate the expression of the CYP19 gene in granulosa cells ofhypothyroid rats induced by propylthiouracil (PTU). This was quasi experimental study withpost-test only control group design. Eleven female Sprague-Dawley rats were divided into twogroups i.e. five rats as treated group that induced by PTU 0.1 g/L in aquadest for 30 days andcontrol group that not induced by PTU. Blood sample was taken and then T4 blood level wasmeasured using an enzyme-linked immunosorbent assay (ELISA). Whereas, CYP19 gene expressionin ovarian granulose cells was measured using immunohistochemistry. Unpaired t test was usedto compare the data obtained from treated and control groups. The results showed that T4blood level on treated group (4.02 ± 0.39 ng/dL) was significantly lower than control group(8.08 ± 1.63 ng/dL) (p = 0.000). However, CYP19 gene expression on treated group (30.84 ±8.01%) was not significantly different compare to control group (25.06 ± 6.79%) (p = 0.227).In conclusion, the CYP19 gene expression in ovarian granulose cells of rats is not change afterinduction of PTU 0.1 g/L for 30 days, although the T4 blood level decreases.
Optimal time of administration of fentanyl in reducing hemodynamic response in endotracheal intubation Sri Rahardjo, Guntur Muhammad Taqwin Gusti Ngurah Artika
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 46, No 02 (2014)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (591.348 KB) | DOI: 10.19106/JMedScie004602201404

Abstract

Laryngoscopy and endotracheal intubations are frequently conducted in general anesthesia.However, it can stimulate symphatic and sympatoadrenal activities. Several non andpharmacological interventions have been used to reduce the symphatic stimulation. Fentanyl isone of the opioid drugs that frequently used to decrease the cardiovascular responses after theintubations. In order to obtain an optimal effect, fentanyl should be administered in appropriatetime. This study was conducted to compare the time of fentanyl administration at 2, 5 and 7minutes before endotracheal intubation to reduce hemodynamic responses. This was anobervational study with a prospective cohort design on patients who were going to undergoelective sugical using general anesthesia followed laryngoscopy intubation in Dr Sardjito GeneralHospital and met the inclusion and exclusion criteria. The patients were then divided into 3groups i.e patients who received fentanyl 1.5 μg/kg body weight (BW) intravenously (IV) 2minutes (Group A), 5 minutes (Group B) and 7 minutes (Group C) before laryngoscopy intubations.The hemodynmic responses including sistolic and diastolic blood pressure (SBP and DBP), meanarterial pressure (MAP), heart rate (HR) and rate pressure product (RPP) were monitored andrecorded every 1 minute during 7 minutes period. The result showed that fentanyl administration5 minutes before laryngeal intubation was more effective in the decrease hemodynamic responsethat those 2 and 7 minutes. Significantly different in SBP in 2, 3, 4 and 7 minutes observationswas observed (p<0.05). Furthermore, significantly different in MAP in 2, 3 and 4 minutesobservation and in RPP in 1, 2, 3, 4 and 7 minute observation were also observed (p<0.05). Nosignificantly different was observed in HR during observation (p>0.05). In conclusion, theadministration of fentanyl 1.5 μg/kg BW IV at 5 minutes before intubation is more effectiveagainst hemodynamic responses in endotracheal intubation.
Cytotoxicity of α-terpineol in HeLa cell line and its effects to apoptosis and cell cycle Mustofa, Rasuane Noor Indwiani Astuti
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 46, No 01 (2014)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (649.907 KB) | DOI: 10.19106/JMedScie004601201401

Abstract

α-Terpineol is a natural compound of terpenoid alcohols class. However, it can be synthesizedfrom α-pinene of turpentin content. α-Terpineol has been reported as potential anticancer agentdue to its activity on inhibition of cells growth and induction of tumor cell death. However, itsanticancer activity in HeLa cervical cancer cells line has never been studied, yet. The aim of thisstudy was to evaluate the cytotoxicity of α-terpineol and its effects to apoptosis and cell cycle.This was a quasi-experimental study with post-test only with non-equivalent control groupdesign. Cytotoxicity of á-terpineol was evaluated using MTT cell viability assay. The effect of α-terpineol on cell apoptotis was tested using acridine orange-ethidium bromide staining method,whereas its effect on cell cycle was evaluated by flowcytometry method. The results showedthat α-terpineol had cytotoxicity against HeLa cell with an IC50 value about 12.46 μg/mL.Furthermore, α-terpineol induced the HeLa with an IC50 value about 13.12 μg/mL. Cell accumulationat G1 phase during cell cycle after incubation with α-terpineol (52.78)was observed. In conclusion,α-terpineol is potential as an anticancer due to its ability to induce cell apoptosis and to inhibitthe cell cycle at G1 phase.
Organophosphate insecticide susceptible test and transovarial transmission detection of dengue virus on Aedes aegypti in Kendari Tri Baskoro Tunggul Satoto, Muhaimin Saranani Sitti Rahmah Umniyati
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 45, No 04 (2013)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (968.716 KB) | DOI: 10.19106/JMedScie004504201303

Abstract

Dengue Fever (DF) is a disease caused by the dengue virus that transmitted by Aedes aegypti(Ae. Aegypti) and Ae. albopictus. Dengue fever is now one of the most important public healthproblems in Indonesia. Vector control using insecticides is the most important strategy to controlthe DF. Massal fogging and selective abatisation have implementedd intensively to control Ae.aegypti. However after its a long time implementation, mosquitoes resistance and transovarialtransmission have been reported. The aim of the study was to evaluate the susceptibility statusof Ae. aegypti to organophosphate and its ability to transovarial transmit degue virus in KendariCity, South East Sulawesi. This was a observational study using cross-sectional design conductedin high endemic areas (Kadia Village) and low endemic areas (Kambu Village). Susceptibilitystatus of Ae. aegypti larvae from F1’s egg generation was evaluated by biochemic assay, whereasthe transovarial transmission of dengue virus of adult female Ae. aegypti was evaluated byimmunohistochemistry method using head squash preparation. The results showed that the Ae.aegypti larvae resistant was higher in high endemic areas (Kadia Village) (83.33%) than in lowendemica areas (Kambu Village) (60.00%). In addition, transovarial transmission index (TTI) ofAe. aegypti dengue virus in the high endemic areas (26%) was significantly higher than in thelow endemic areas (12%) (p<0.05). In conclusion, the Ae. aegypti larvae resitance toorganophosphate as well as the TTI in high endemic areas is higher than in low endemic area inKendari, Sout East Sulawesi.

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