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Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Published by Universitas Gadjah Mada
ISSN : 01261312     EISSN : 23563931     DOI : http://dx.doi.org/10.19106/JMedSci005303202101
Core Subject : Science,
Immunology & microbiology Neuroscience
Journal of the Medical Sciences (JMedSci) or Berkala Ilmu Kedokteran (BIK) is an international, open-access, and double-blind peer-reviewed journal, published by Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada Yogyakarta Indonesia. JMedSci aiming to communicate high-quality articles in the areas of biomedical science from basic to clinical sciences.The journal welcomes papers from original articles, case reports, reviews, and book reviews. All papers published in JMedSci are freely available as downloadable pdf files. The journal began its publication on March 1973 and published quarterly (January, April, July, and October). JMedSci is abstracted and indexed in DOAJ, Crossref, Google Scholar, Sinta, Indonesia One Search. JMedSci is accredited by Directorate of General Higher Education, the Ministry of Research, Technology, and Higher Education, Indonesia
Arjuna Subject : Kedokteran - Kedokteran (Lain-Lain)
Articles 2,170 Documents
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Pigmented Gallstone in Children With Thalassemia Syndrome Sustiyanto, Nenny SM Sumadiono
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 39, No 02 (2007)
Publisher : Universitas Gadjah Mada

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Abstract

Background: Pigmented gall stones in children frequently occur in spherocytosis and sickle cell anemia. The most common congenital hemolytic anemia in Indonesia is thalassemia. There is only a few studies concerning pigmented gall stones in thalassemia in the world and none in Indonesia. This study was aimed to know the prevalence of pigmented gall stones in patients with thalassemia in Pediatric Department of Dr. Sardjito Hospital.Materials and method: All patients with major thalassemia visiting Dr. Sardjito Hospital who consented to take part in the study underwent ultrasonography to identify the stone or sludge. As controls, children with no jaundice were examined.Results. There was 8,57% gallstone in thallasemia group and none in the control group.Conclusion: The occurrence of pigment gallstone in the thalassemia syndrome children in Yogyakarta the same as in other places. The risk factors of pigment gallstone should be investigated.Key words: gall stone - prevalence - thalassemia syndrome - ultrasonography.
Low-dose short-course metformin decrease the risk of atherosclerosis in the non-obese prediabetic individuals Djoko Hardiman, Djoko Hardiman
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 40, No 03 (2008)
Publisher : Universitas Gadjah Mada

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Abstract

Background: The establishment of macrovascular (cardiovascular) event is initiated in the period of prediabetic. Metformin plays role in the carbohydrate and lipid metabolism as well as vascular protection. The mechanism of the cardiovascular event risk increased in the pre-diabetic individual has not been clearly established.Objective: The study was to determine if insulin resistance control by low-dose short-course metformin administration in the non-obese prediabetic individual might decrease the risk of atherosclerosis.Methods: It was a prospective experimental double blind study with pretest-posttest control group design. It was conducted on the first degree relatives of type-2 Diabetes Mellitus patients who met the criteria of non-obese prediabetic individuals. Subjects were randomly classified into treatment group (designed for metformin administration), and the control group (designed for placebo administration). The prediction of atherosclerosis risk was based on the hsCRP, PAI-1, VCAM-1, and fibrinogen levels.Result: The results showed that the margin between pre- and post-metformin administration term were 1.89 vs. 0.06 mg/L (p=0.001), 1.42 vs. 0.84 IU/mL (p=0.0151. 180.85 vs. 03.81 mg/L (p=0.061) and 80.15 vs. 31.42 mg/dL (p=0.001) for hsCRP, PAI-1, VCAM-1, and Fibrinogen levels in the treatment and control group, respectively.Conclusion: The study concluded that the low-dose short-course metformin administration might decrease the atherosclerosis risk significantly in the non-obese prediabetic individuals.Key words: metformin - non-obese prediabetics - hsCRP - PAI-T- Fibrinogen - VCAM-T
Nonmosaic 45, XO karyotype in a woman with Turner syndrome without any cognitive, psychosocial or behavioral deficiencies (A Case report) Djaswadi Dasuki, Enny S Pamuji
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 39, No 03 (2007)
Publisher : Universitas Gadjah Mada

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Abstract

A 22 year old woman with primary amenorrhea came to The Reproductive Endocrinology and Infertility Centre, University of Gadjah Mada, Dr.Sardjito Hospital. She had no abnormalities in cognitive, psychosocial or behavioral capacities, she had almost completed her university degree. On examination she had no secondary sexual development, no pathognomonic abnormal phenotype related to abnormal karyotype. There was no indication of family history associated ,;pecifically to the abnormal karyotype, phenotype feature of Turner syndrome and a specificThe patient karyotype analysis of blood sampling indicated 45XO, and it failed to demonstrate any mosaicism. Laparoscopic examination revealed a hypoplastic-lobulated-unicorns uterus, as streak gonad at the left side and no ovary at the other side.Keywords: Turner syndrome, non behavioral deficiencies. nonmosaic 45, XO karyotype, normal hormone level
The relationship between vitamin A and ferritin towards malondialdehyde level among Javanese male smokers Ahmad Hamim Sadewa, Ajeng Viska Icanervilia Sunarti
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 44, No 01 (2012)
Publisher : Universitas Gadjah Mada

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Abstract

Cigarette smokes produce a large number of oxidants and promote secretion of ferritin byalveolar macrophages which are potential to encourage the lipid peroxidation. Malondialdehyde(MDA) is used as a parameter of lipid peroxidation. The study was aimed to evaluate therelationship between blood level of vitamin A and ferritin and MDA among Javanese malesmokers. Sixty men who lived in Purworejo District, Central Java, Indonesia comprising 30smokers as case group and 30 nonsmokers as control group were involved in this study. Bloodsample was obtained from cubiti vein and then centrifuged to obtain plasma or serum. Blood levelsof vitamin A, ferritin and MDA were measured by HPLC, ELISA and spectrophotometric methods,respectively. The result showed that the blood vitamin A, ferritin, and MDA levels in smokers were25.09±9.51μg/dL, 35.50±24.17ng/dL, 1.15±0.42μg/L, respectively, whereas in non smokers, theywere 26.11±9.19 μg/dL, 38.60±15.25 ng/dL, 1.06±0.50 μg/L, respectively. Therewas no significantdifference of the blood vitamin A, ferritin, and MDA levels between smokers and the non smokers(p>0.05). The linear regression analysis indicated that there was negative relationship betweenblood vitamin A and MDA levels although it was not significant (p=0.052), while blood ferritin andMDA levels had a significantly positive relationship (p=0.010). In conclusion, the low level of bloodvitamin A among cigarette smokers does not lead to high blood MDA level, while high level of bloodferritin among smokers leads to high blood MDA level.Keywords: vitamin A - ferritin - malondialdehyde - cigarette - smokers
Comparison of postthawing sperm motility recovery between cryopreserved with and without cryoprotective agent using 4 different cryopreservationmethods Irwan Taufiqur Rachman, Hilwah Nora Shofwal Widad
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 44, No 01 (2012)
Publisher : Universitas Gadjah Mada

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Abstract

Frozen-thawed human spermatozoa are routinely used for many assisted reproduction program.However, cryopreserved spermatozoa was reported to yield lower pregnancy rates compared tofresh semen in both intra uterine inseminations and in vitro fertilization/intracytoplasmic sperminjection (IVF/ICSI) due to the reduction of sperm motility and viability induced by cryopreservationprocedures. This study was aimed to evaluate the influence of cryoprotective agent (CPA) andcryopreservation methods on human sperm motility. This was a quasi experimental study. Thirtyseven normozoospermic semen samples collected in Permata Hati Infertility Clinics of Dr. SardjitoGeneral Hospital, Yogyakarta were recruited. Four different cryopreservation methods were appliedusing and without CPA (TEST-yolk buffer). In simple two steps freezing, cryostraw were graduallyfrozen from 8 to -4°C. In simple graduated freezing, cryostraus were directly frozen at -4°C. Invapor phase freezing method, the samples in cryostraw were placed 1 cm above liquid nitrogen.In the last method, the samples were directly submerged into liquid nitrogen. Thawing wasconducted by incubation at 37°C for 5 minutes. The sperm motility recovery after cryopreservationin the 4 different cryopreservation methods was evaluated and analyzed by analysis of variance(ANOVA). The fresh sperm motility before cryopreservation was 52.9 ± 4.50%. The recovery ofmotile sperms was 17.00 ± 7.83%, 20.96 ± 5.81%, 15.06 ± 8.55% and 15.68 ± 8.3%, whenusing CPA and 5.63 ± 4.63%, 5.47 ± 3.95%, 4.45 ± 4.46% and 6.08 ± 5.06% when withoutCPA following direct plunge to liquid nitrogen freezing, vapor liquid nitrogen freezing, simplegraduated freezing and simple 2-steps freezing, respectively. Among methods using CPA, thevapor phase method resulted in highest sperm motility recovery. In methods without CPA, nosignificant difference of sperm motility recovery was observed among the 4 differentcryopreservation methods. In conclusion, the use of CPA for cryopreservation improves spermmotility recovery.
Natural Killer (NK) cells and severity of dengue infection among children hospitalized in Dr. Sardjito Hospital Ida Savitri Laksanawati, Umi S. Intansari
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 40, No 02 (2008)
Publisher : Universitas Gadjah Mada

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Abstract

Background: Dengue infection isONE OF the most frequent infectious diseases in Indonesia. The manifestation of dengue infections varies from asymptomatic to live-threatening, dengue shock syndrome. It is hypothesized that immune responses contribute to immunopathogenesis of severe dengue infection. Natural killer INK) cells are innate lymphocytes which play a crucial role in anti-pathogen host defense and are particularly important in mediating resistance to viral infections. However, only a few studies of NK cells have been conducted in vivo, especially in Indonesia.ObJectives: The objectives of this study were to find the correlation between the number of NK cells with severity of the disease, and to find out the profile of NK cells absolute numbers in acute and convalescent dengue infection patients among children hospitalized n Dr. Sardjito hospitalMethods: An observational prospective study was conducted. Clinical and laboratory data were followed from the day of admission until the day of discharge. The severity of the disease was graded according to the WHO criteria for Dengue Hemorrhagic Fever IDHF). The absolute number of NK cells was measured by flow cytometer using monoclonal antibody anti-CD3, anti-CD16 + 56, and anti-CD45, with dual platform method. Student t-test was used to find out the difference of NK cell absolute number between acute and convalescent phases. The correlation between NK cells and the severity of the disease was analyzed using Spearman correlation test.Results: NK cell absolute number tended to be lower in acute compared to convalescent phase, but the difference was not statistically significant. There was an inverse correlation between the NK cell absolute number and the severity of dengue infection Ir=-O.322; p=O.017).Conclusion: Absolute number of NK cells were decreased during acute phase of dengue infection, and the decrease was more pronounced in severe than in mild dengue infection.Key words: NK cells absolute number - mild and severe dengue infection - acute and convalescent phases
Cytotoxic Activity of /V-alkyl and /V-benzyl 1,10-phenathroline derivatives in Human Cancer Cell Lines Isnatin Miladiyah, Esti Nurwening Sholikhah Mustofa
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 39, No 01 (2007)
Publisher : Universitas Gadjah Mada

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Abstract

Background: In our study on the antiplasmodial activity of 1,1 O-phenanthroline derivatives, we found some compounds possessing a potential cytotoxic in normal cell line.Objective: In this study we tested these derivatives in human cancer lines in order to know their in vitro anticancer activity.Materials and methods: Six derivatives of 1,1 O-phenanthroline, 4 derivatives of N-alkyl and 2 derivatives of N-benzyl 1,1 O-phenanthroline were tested on two human cells cancer, myeloma (NS-1) and HeLa cells line. Cytotoxic activity was evaluated by trypan blue exclusion assay and their activity was expressed by the concentration inhibiting 50% of the cell growth (lCso) The ICso of each compound was determined by probit analysis.Results: The results showed that the ICso values of 1,1 O-phenanthroline derivatives ranged from 4.68 to 15.63 pM on myeloma cell and from 2.82 to 16.89 pM on HeLa cell. The 4-Bromo-3(2-bromoetyll-2metyl-1 , 1 O-phenantroline (3) and (1 )-N-(4-benzyloxy-3-methoxy-benzyll-1 ,1 O-phenanthrolinium chloride (6) with ICso values ranged from 4.68 to 4.72 pM on myeloma cell showed the same (p>0.05) cytotoxicity with doxorubicin, with ICso values ranged from 2.82 to 3.08 pM on HeLa cell showed the higher (p
Premenstrual syndrome (PMS) and premenstrual dysphoric disorder (PMDD) in Indonesian women Ova Emilia, Ova Emilia Ova Emilia
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 40, No 03 (2008)
Publisher : Universitas Gadjah Mada

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Background: Data from epidemiologic surveys showed that the burden of illness caused by premenstrual syndrome could not be underestimated. It has been estimated that the frequency of premenstrual symptoms was quite high (80-90%1, and in some women, the symptoms were so severe that they might interfere with daily activities. Most epidemiological studies of PMS were conducted in Western women, though a few small sample-size studies of Chinese and Japanese women indicated that they were affected by PMS. Objective: To find out the characteristic features of premenstrual symptoms in Indonesian women.Methods: Four hundred and sixty women aged between 20 and 49 yrs. with regular menstrual cycles, who were seen at a Women Health Clinic, were assessed regarding their premenstrual symptoms using the Premenstrual Symptoms Screening Tool (PSSTI. They were able to provide informed consent. The study was conducted in July 2007 until January 2008.Results: As many as 95% of these women were found to have at least premenstrual symptoms. The rates of prevalence of moderate to severe PMS and PMDD in this study were 3.9% and 1.1 %, respectively, which were lower than those in Western or Chinese and Japanese women. Only 5 women with moderate to severe PMS and PMDD were treated and 10.5% women seek herbal medicine to reduce premenstrual symptoms.Conclusion: Severe to moderate premenstrual symptoms and PMDD were suffered by 5% women and mostly affected 20-29 year old women. Medical treatment was rarely sought, but herbal medicine was often used to relieve premenstrual symptoms.Key words: PMS - PMDD - Indonesian women
Relation between vitamin C and vitamin E levels with malondialdehyde in elderly Irianti Puji Lestari, Prasetyastuti Sunarti, Dyah Arum Retnaningstyas
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 39, No 03 (2007)
Publisher : Universitas Gadjah Mada

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Background: Aging is a natural process during life. One of the aging theories is related to free radical production. Increase of free radical production can trigger lipid peroxidation that result in peroxide lipid such as malondialdehyde (MDA). The lipid peroxidation can be inhibited by body defence system such 8S vitamin C and vitamin E.ObJective: to understand the relation between plasma vitamin C, vitamin E and MDA levels in elderly Method: This was an observational research with cross sectional design. The subjects were thirty two elderly who live in Pakem district, Sleman. Determination of vitamin C and peroxide lipid level used spectrophotometer, whereas vitamin E level was determined by spectrofluorometer.Reaulta : The concentration of vitamin C , vitamin E and MDA of the subjects were 0.50 + 0.26 mglml, 26.38 ..:!:. 7.95 pg/ml and 0.23 ..:!:. 0.13 nmol/ml respectively. The correlation of both vitamin C vs MDA and vitamin E vs MDA were not significant, r = -0.012 and -0,108, respectively.Conclualon: There were no correlation between vitamin C, vitamin E and MDA levels.Keyword: elderly ; free radical;vitamin C; vitamin E; malondialdehyde.
The effect of chronic ethanol administration on total number of pyramidal cells in CA 1 and CA3 of hippocampus and spatial working memory in adolescent rats (Rattus norvegicus) Mustofa, Muh. Ihwan Nurwanto Soejono Aswin
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 40, No 04 (2008)
Publisher : Universitas Gadjah Mada

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Background: Adolescent brain may be more susceptible to damage by ethanol. Chronic ethanol administration may cause morphological changes in the hippocampus, followed by deficit in the hippocampal function, including the impairment of memory.Objective: The aim of this study were to investigate the changes in the total number of pyramidal cells in CA 1 and CA3 of hippocampus, and the relationship between these changes with spatial working memory of rats after chronic ethanol administration at adolescent age.Methods: Twenty five adolescent (30 days of agel male rats (Rattus norvegicusl were classified randomly into 5 groups, that is, namely control without treatment (C1 I, control with treatment (C21, treatment 1(11 I, treatment 2 (T2), and treatment 3 (T31 groups. Each group consisted of 5 rats. C2 group was given physiological saline, while 11, T2, and T3 groups were given ethanol at various doses of 1, 2, and 3 gl kgBW/day, repectively, for 30 days by intraperitoneal injection. After chronic ethanol administration, the spatial working memory of 5 groups were tested using 8 arms radial maze for 12 days. At the end of the experiment, the rats were sacrified and the brains were taken out for histological examination, by paraffin method and Cresyl violet staining.Result: Chronic ethanol administration on adolescent rats caused a decrease in spatial working memory (especially in group T31 (p < 0.051. The total number of pyramidal cells in CA 1 of group T3 was fewer than those of control groups (p < 0.05), but the total numbers of pyramidal cells in CA3 of treatment groups were not different with those of control groups (p > 0.051. Analysis with product moment correlation test showed that there was a positive correlation between the total number of pyramidal cells in CA 1 of hippocampus with spatial working memory of the rats after chronic ethanol administration at adolescent age (p < 0.051.Conclusion: Chronic ethanol administration on adolescent rats caused a decrease in the spatial working memory and the number of pyramidal cells in CA 1 of hippocampus. There was a positive correlation between the number of pyramidal cells in CA 1 and spatial working memory of the rats after chronic ethanol administration at adolescent age.Key words: ethanol - pyramidal cells - spatial working memory - adolescent

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