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INDONESIA
Jurnal Sains Dasar
Published by Universitas Negeri Yogyakarta
ISSN : 20859872     EISSN : 24431273     DOI : 10.21831
Core Subject : Education,
Education
Arjuna Subject : -
Articles 242 Documents
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Characterization and Identification of Indol Acetic Acid Phytohormone-producing Bacteria from Corn Root (Zea mays L.) Putri Maharani, Rendha; Aeni Ariyanti, Nur; Aloysius, Suyitno; Sugiyarto, Lili
Jurnal Sains Dasar Vol. 14 No. 2 (2025): October 2025
Publisher : Faculty of Mathematics and Natural Science, Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Rhizobacteria are endophytic bacteria that inhabit plant tissues and the rhizosphere, producing secondary metabolites that support plant growth. This study is an exploratory descriptive investigation aimed at characterizing and identifying IAA- and phytohormone-producing endophytic rhizobacteria isolated from the roots of corn plants (Zea mays L.). The rhizobacteria isolates producing IAA phytohormones were obtained by culturing bacteria in Yeast Peptone Broth (YPB) medium supplemented with L-tryptophan. Bacterial cultures (1 mL) were treated with Salkowski reagent, and absorbance was measured spectrophotometrically at a wavelength of 530 nm. From 14 isolates, five were identified as producing the highest levels of indole-3-acetic acid (IAA), ranging from 17 to 50 ppm. Identification was conducted using the profile matching method to determine the genus of each rhizobacterium by comparing its characteristics with reference genera. Based on 62 tested traits, the five isolates exhibited diverse features, including Gram-positive and Gram-negative bacteria, coccus-shaped morphology, circular and irregular colony configurations, lobate and undulate margins, convex, umbonate, and raised elevations, and colony colors ranging from white to yellowish, along with varied physiological (biochemical) test results. The identification results revealed bacteria with a similarity index of ≥70%, suggesting proximity to the genus Micrococcus (77.4%, isolate N), Rhizobium (80.6%, isolate D), and Shewanella (80.6%, isolate B; 87.1%, isolate V; and 91.9%, isolate E). This research provides an important contribution to the development of biofertilizers aimed at enhancing the growth and productivity of corn plants sustainably.
Physical Properties of Durian (Durio zibethinus Murr) Seed Strach and its Associated Edible Film Wattimena, Synodalia C.; Patty, Philip J.
Jurnal Sains Dasar Vol. 14 No. 2 (2025): October 2025
Publisher : Faculty of Mathematics and Natural Science, Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/jsd.v14i2.90720

Abstract

Starch has become a popular material as a substitute for conventional crude oil in plastic production. An edible film, a transparent thin film made from biobased materials, is one product that mostly uses starch. In this study, the starch of durian (Durio zibethinus Murr) fruit seed was used to make an edible film, and the physical properties of the starch and its associated edible film were characterized. A light microscope was used to observe the shapes and size distribution of the granules, a basic component of the starch consisting of linear amylose and branched amylopectin chains, and XRD spectroscopy was used to characterize the crystalline properties of the starch. Most of the granules were in spherical shapes with some irregularities, and the diameter ranged from 1.11 to 6.67 µm. Based on the XRD data, durian seed starch can be classified as a B-type starch with a crystallinity index of 22.2%. An edible film made from durian seed looks transparent brownish with dark brown spots. Its water vapor transmission rate was 2.49±0.01 g/m²hour, water absorption 145.1±34.9%, and water solubility 13.36±0.86%.

Page 25 of 25 | Total Record : 242

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