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Contact Name
Brigitta Laksmi Paramita
Contact Email
brigitta.laksmi@uajy.ac.id
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+6282329549978
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journal.biota@gmail.com
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Fakultas Teknobiologi, Universitas Atma Jaya Yogyakarta, Jalan Babarsari No. 44, Sleman, Yogyakarta 55281, Indonesia
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Kota yogyakarta,
Daerah istimewa yogyakarta
INDONESIA
Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati
ISSN : 25273221     EISSN : 2527323X     DOI : doi.org/10.24002/biota
Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati merupakan jurnal ilmiah yang memuat hasil-hasil penelitian, kajian-kajian pustaka dan berita-berita terbaru tentang ilmu dan teknologi kehayatian (biologi, bioteknologi dan bidang ilmu yang terkait). Biota terbit pertama kali bulan Juli 1995 dengan ISSN 0853-8670. Biota terbit tiga nomor dalam satu tahun (Februari, Juni, dan Oktober).
Articles 20 Documents
Search results for , issue "Vol 10, No 2 (2005): June 2005" : 20 Documents clear
Penelitian Tentang Biologi Reproduksi pada Brill (Colistium guntheri Hutton, 1926) di Perairan Otago Selatan, New Zealand Setyono, Dwi Eny
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 2 (2005): June 2005
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (577.827 KB) | DOI: 10.24002/biota.v10i2.2842

Abstract

Perkembangan gonad, musim pemijahan dan fekunditas ikan sebelah “brill” (Colistium guntheri Hutton, 1926) di perairan Otago Selatan, New Zealand telah diamati selama satu tahun. Sampel ikan ditangkap setiap bulan menggunakan “bottom otter trawl”. Indeks gonad, tingkat kematangan gonad dan distribusi frekuensi ukuran telur digunakan untuk menentukan perkembangan gonad dan musim pemijahan. Hasil penelitian ini menunjukkan bahwa perbandingan populasi ikan “brill” jantan dan betina tidak berbeda nyata dari 1 : 1. Pada ukuran panjang tubuh yang sama, ikan betina mempunyai berat badan yang lebih besar dari pada ikan jantan. Hasil analisa histologi menunjukkan bahwa C. guntheri termasuk kelompok ikan yang mempunyai perkembangan telur secara sinkroni. Berdasarkan perubahan nilai indeks gonad dan perkembangan ovari, jenis ikan ini diketahui mengalami pematangan gonad yang sangat cepat pada akhir musim gugur (Juni), yaitu ditandai dengan peningkatan nilai indeks gonad secara drastis dan ovari didominasi oleh telur pada tingkat kematangan akhir (final maturation stage). Musim pemijahan dimulai pada akhir musim dingin (Agustus) sampai musim panas (Januari), ditandai dengan penurunan nilai indeks gonad secara nyata dan ovari didominasi oleh telur yang telah masak (mature), hidrasi (hydrated) dan paska ovulasi (postovulatory). Fekunditas (batch fecundity) ditentukan dengan menghitung semua telur yang telah masak, mencakup sekitar 34% dari total telur di dalam ovari. Pada pengamatan ini diketahui bahwa fekunditas (Y) proporsional dengan berat gonad dalam gram (Wg) dengan persamaan regresi Y = 103 [(4.34 + 20.06 (Wg)], R2 = 0.95, dan fekunditas relatif per gram berat gonad adalah 18.760+1.150 telur.
Pemanfaatan Oleoresin Jahe (Zingiber officinale) untuk Mengatasi Kelainan Antioksidan Intrasel Superoxide Dismutase (SOD) Hati Tikus Di Bawah Kondisi Stres Wresdiyati, Tutik; Astawan, Made; Adnyane, I Ketut Mudite; Prasetyawati, Renny Candra
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 2 (2005): June 2005
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (266 KB) | DOI: 10.24002/biota.v10i2.2848

Abstract

This study was conducted to evaluate the activity of ginger oleoresin (Zingiber officinale) on the intracelluler antioxidant-superoxide dismutase (SOD) in the liver of rats under stress condition. A total of twuenty five Wistar rats were used for this study. They were divided into five groups ; (1)K (control), (2) S (stress), (3)O + S (oleoresin followed by stress), (4) S + O (stress followed by oleoresin and (5) O + S +O (oleoresin followed by stress then oleoresin). The dose of oleoresin is 60mg/Kg/BW/day for seven days. Stress condition was done by five days fasting and smimming for five minutes/day, while drinking water was provided ad libitum to all groups. The results showed that ginger oleoresin significantly decreased malonaldehyde (MDA) and elevated SOD activity. The immunohistochemical evaluation also showed that ginger oleoresin increased the content of copper,zinc-superoxide dismutase (Cu,Zn-SOD) in the liver of rats under stress condition. These effects were showed in the tissues of rats treated by ginger oleoresin before or after stress or combination of both.
Peningkatan Produksi Asam Glutamat Corynebacterium glutamicum dengan Penambahan Penisilin pada Fase Logaritmik Mursyanti, Exsyupransia; Lestari, Sri
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 2 (2005): June 2005
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (437.3 KB) | DOI: 10.24002/biota.v10i2.2843

Abstract

One way to increase production and excretion of glutamic acid was to increase cell's permeability. Penicillin has a potency to change the cell permeability by inhibitng cell wall synthesis. However, penicillin treatment was effective only for actively dividing cells. Therefore, such a research was done to study on the time of penicillin treatment to the medium, so that it can be found optimal cell biomass to produce maximum glutamic acid. The cell utilized in the research was Corynebacterium glutamicum IFO 12168 that was in batch cultured. Concentration of penicillin added was 5 unit/ml and treated at incubation periods 12, 14, 16, 18, and 20 hours, respectively, after inoculation. The steps of the research were as follows purification test, growth pattern, and glutamic acid production. Parameters measured at the end of the fermentation were cell biomass, reduced sugar concentration, medium’s pH, and glutamic acid concentration. Data was analysed utilizing Anova and the significant difference between treatments were tested using Duncan’s Multiple Range Test (DMRT). The growh pattern shown that logarithmic phase was reached at 2 to 22 hrs of incubation periods, therefore the treatment of penicillin was given at 12, 14, 16, 18, and 20 hrs of incubation periods. Cell biomass produced was corelate with the concentration of reduced sugar in the medium. Measured pH of the medium at the end of the fermentation was on the pH range for the growth of C. glutanicum. The research concluded that Penicillin treatment was able to increase significantly the glutamic acid production compatred to control treatment. Time accuracy of penicillin treatment to produce maximum glutamic acid (154319,60 µg/ml) was on 18 hrs of incubation period.
Dengan Rumput Laut, Membangun Negara (Kajian Buku) Sidharta, Boy Rahardjo
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 2 (2005): June 2005
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (154.606 KB) | DOI: 10.24002/biota.v10i2.2850

Abstract

Rumput laut (seaweed) adalah biota laut yang mungkin paling dikenal manusia sejak lampau (setelah ikan, tentunya). Manfaat rumput laut bagi manusia juga sangat bervariasi, mulai dari sumber makanan hingga obat-obatan. Di dunia moderen sekarang ini, rumput laut juga semakin dikenal berkat hasil-hasil penelitian ilmiah yang dilakukan manusia terhadap biota ini.
Induksi Pembungaan Rambutan dengan Aplikasi Paklobutrazol Prawitasari, Theresia; Dorly, Dorly; Wahyuni, Sri
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 2 (2005): June 2005
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (351.556 KB) | DOI: 10.24002/biota.v10i2.2845

Abstract

Rambutan fruit have a great potential to be developed as an exported commodity. The constraint is the existence of biannual bearing characteristic of the plant, which is an alternation of fruit yield between on year and off year. This condition resulted in a problem in product availability continuity. The aim of this research was to induce rambutan plant flowering in the off year with the application of paclobutrazol. There were three doses of paclobutrazol treatment including 0 g/tree, 1.5 g/tree, and 3 g/tree. Bud dormancy as an implication of paclobutrazol application were broken by the application of KNO3 at 20 g/l concentration. The experiment was arranged in completely randomized factorial design 3X2 in triplicate. The result showed that paclobutrazol treatment was able to induce rambutan flowering in the off year. Paclobutrazol treatment significantly increased the number of generative buds. Based on the generative bud percentage, the higher doses of paclobutrazol application the stronger stimulus of flowering. Paclobutrazol treatment also reduced the inflorescences length and increased the chlorophyll content of the flowering support leaves. It seems that application at dose 3 g/tree caused bud dormancy but dose 1.5 g/tree did not. Bud dormancy as a result of paclobutrazol application could be broken by the KNO3 application. The optimum dose to induced rambutan flowering was 1.5 g/tree because this dose seems did not caused bud dormancy and gave a higher number of generative buds compared to 3 g/tree dose.
Aktivitas Antibakteri Ekstrak Metanol Buah Mbosi (Dysoxylum gaudichandianum (A.Juss) Miq.) dan Penapisan Senyawa Kimianya Praptiwi, Praptiwi; Harapini, Mindarti
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 2 (2005): June 2005
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (162.42 KB) | DOI: 10.24002/biota.v10i2.2844

Abstract

Mbosi (Dysoxylum gaudichandianum (A.Juss) Miq.) belongs to Meliaceae family, Its potential bioactivity had not been known although the other plants from the same family had been known as natural insectiside or medicinal plants. Phytochemical screening showed that mbosi fruit contained fat, fatty acid, sterol, triterpenoide, tannin, peroxide sugar, alkaloid, steroid glycoside and flavonoid. Identification of mbosi fruit chemical compounds by GC-MS showed that it contained 16 compounds. Compounds with the concentration more than 5 % were butyl cellosolve (11.1%), erythritol (8.48%), glycerol (10.32%), hexadecanioc acid palmitinic ester (16.2%), oleic acid (65.3%) and ethyl oleat (6.2%).  In-vitro antibacterial test on innoculated Mueller Hinton Agar to seven bacteria isolates (Salmonella typhimurium, S. typhii, Eschechiria coli ATCC 25923, Pseudomonas sp., Staphylococcus aureus ATCC 25922, S. epidermidis, and Bacilus subtilis)  showed that 20% methanol extract  inhibit only the growth of  Staphylococcus aureus.
Kajian Awal Pemanenan Siput Laut (Gastropoda) di Pantai Krakal, Yogyakarta III: Aktivitas Wisatawan Andi, Santi Ari; Zahida, Felicia; Sidharta, B. Boy
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 2 (2005): June 2005
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (165.965 KB) | DOI: 10.24002/biota.v10i2.2847

Abstract

This study wanted to elucidate the role of tourists activities to the beach such as Krakal Beach, Yogyakarta. The method used in this research was direct interview to the visitors using accidental sampling with the help of prepared questionnaire. This study showed that 86% visitors were in their school age. Person in this age like such a challenge like what they met during beach visit. Those make the visitation frequency quite high by repeating their visit (53,5%). Shells and shells handicrafts has been proven to be the reason why visitors come back to visit the beach (79%). Apparently, visitors agree that the snails’ forms and colors are attactive (70,9%), beautiful and unique (75,6%), but only 14% said that it shouldn’t be disturb by human activities. Their attractiveness to the snails did not make them to collect intentionally, enough for them to see and let them go (66,2%). They did not agree that visitors collected them (51,2%), but they agree harvesters make a souvenirs and get an income from this activity (67,4%). In short, they were much environment friendly (57%) rather than economic friendly (43%).  
Peluang Penggunaan Spermatozoa Epididimis Yang Dikoleksi Setelah Kematian Sebagai Sumber Sel Gamet pada Anjing Puja, I Ketut; Trilaksana, IGN; Lontoh, Rudy
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 2 (2005): June 2005
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (50.434 KB) | DOI: 10.24002/biota.v10i2.2846

Abstract

The purpose of this study was to determine the effect of postmortem time on percentage of life epididymal sperm from postmortem canine caudae epididymides. A total of 9 dog were used and divided into three group. T0 was control group, T1, 3 hours postmortem and T2, 6 hours postmortem. This way, samples were obtained at different times postmortem. Sperm were extracted from the caudae epididymis by means of cuts. The result showed that the percentage of life sperm were 67,16 ± 5.67(T0), 46.33± 5.60 (T1) and 24.00 ± 4.35 respectively. We could appreciate that percentage of life was affected by postmortem time. There was significant decrease life sperm recovered from epididymides postmortem (P<0.01). In conclusion, epididymal sperm from dog undergo decrease of percentage of life, but it could stay acceptable within many hours postmortem. We intepreted these data to indicate that it may still be possible to obtain viable spermatozoa many hours later.
Studi Komparasi Keanekaragaman Benthos Di Waduk Sempor, Waduk Kedungombo dan Waduk Gajahmungkur, Jawa Tengah (Short Communication) Jati, Wibowo Nugroho
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 2 (2005): June 2005
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (55.038 KB) | DOI: 10.24002/biota.v10i2.2849

Abstract

Selama ini pengelolaan sumberdaya alam hanya didasarkan pada pemenuhan aspek ekonomi serta hubungan yang bersifat fisik (physical interrelationship). Hal ini dapat diperhatikan adanya kecenderungan pengelolaan sumberdaya alam sebatas program konservasi lingkungan fisik seperti hutan, tanah, air dan bukan pengelolaan sumberdaya alam yang bertujuan untuk keberlanjutan sumberdaya alam (natural resources sustainability). Dengan demikian maka dapat dipastikan lambat laun atau cepat akan terjadi degradasi atau penurunan kualitas lingkungan yang pada akhirnya dapat menimbulkan bencana ekologis (Hadisusanto et al., 2003).
Prospek Pengembangan Metode Identifikasi Spesies Burung berdasarkan Spektrum Suara menggunakan Analisis Spektogram Irwandi, Irwandi; Marwan, Marwan; Mahmud, A. Hadi; Abdullah, Abdullah
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 2 (2005): June 2005
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (388.225 KB) | DOI: 10.24002/biota.v10i2.2841

Abstract

Bird species can be identified using bird’s sound. Research about sound bird identification is lacking in Indonesia. This research is initial study to classify bird based on sound and its spectrum. The research was done in Laboratory of Animal Anatomy to record bird sample sound and Laboratory of Computation Physics for data analysis. This research used five recorded sound birds, i.e. Blue-Crowned (Loriculus galgulus), Greater Green (Chloropsis sonnerati), Black-Crested (Pycnonotus melanicterus), Long-Tailed (Lanius schach), and Asian Pied Starling (Sturnus contra). Identification of bird sound spectrum has been done using Fast Fourier Transform (FFT) method. Bird sounds and spectral that plotted in time-domain and in frequency-domain, were indicated unique pattern lead to bird identification method. The result showed that five birds recorded had different sound characteristics, both in time domain and sound spectrum frequency domain. The sound spectrum identification method can be used as initial step toward bird species classification called sono-taxonomy.

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