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INDONESIA
Indonesian Journal of Agricultural Science
Published by Kementerian Pertanian
ISSN : 1411982X     EISSN : 23548509     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
The journal publishes primary research articles from any source if they make a significant original contribution to the experimental or theoretical understanding of some aspect of agricultural science in Indonesia. The definition of agricultural science is kept as wide as possible to allow the broadest coverage in the journal. Accredited base on Director Decree of Indonesian Institute of Science No. 816/D/2009 with acreditation No. 172/AU1/P2MBI/08/2009. IJAS is newly accredited by the Indonesian Institute of Science in 2012 (No. 473/AU2/P2MI-LIPI/08/2012) for period of 2012-2015.
Arjuna Subject : -
Articles 352 Documents
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THE USE OF gusA REPORTER GENE TO MONITOR THE SURVIVAL OF INTRODUCED BACTERIA IN THE SOIL Edi Husen
Indonesian Journal of Agricultural Science Vol 6, No 1 (2005): April 2005
Publisher : Indonesian Agency for Agricultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/ijas.v6n1.2005.p32-38

Abstract

An effective marker to monitor the survival of introduced bacteria in the soil is required for further evaluation of their beneficial effects on plant growth. This study tested the use of gusA gene as a marker to trace the fate of three Gram negative bacteria in the root, rhizosphere, and soil. The study was conducted at the laboratory and greenhouse of the National Institute of Molecular Biology and Biotechnology, Philippines from January to December 2001. Isolates TCaR 61 and TCeRe 60, and Azotobacter vinelandii Mac 259 were selected as test bacteria based on their ability to produce indole-3acetic acid and solubilize precipitated phosphate, which may promote plant growth in the field. These bacteria were marked with gusA reporter gene from Escherichia coli strain S17-1(λ-pir) containing mTn5SSgusA21. The gusA (β-glucuronidase) gene from the donor (E. coli) was transferred to each bacterium (recipient) through bacterial conjugation in mating procedures using tryptone-yeast agar followed by the selection of the transconjugants (bacteria receiving gusA) in tryptone-yeast agar supplemented with double antibiotics and X-GlcA (5bromo-4chloro- 3indoxyl-β-D-glucuronic acid). The antibiotics used were rifampicin and either streptomycin or spectinomycin based on antibiotic profiles of the donor and recipients. The results showed that the insertion of gusA gene into bacterial genomes of the recipient did not impair its phenotypic traits; the growth rates of the transconjugants as well as their ability to produce indole-3acetic acid and solubilize precipitated phosphate in pure culture were similar to their wild types. All transconjugants colonized the roots of hot pepper (Capsicum annuum L.) and survived in the rhizosphere and soil until the late of vegetative growth stage. The distinct blue staining of transconjugants as the expression of gusA gene in media containing X-GlcA coupled with their resistance to rifampicin and streptomycin or spectinomycin made them easier to be recognized and evaluated.
CHARACTERISTICS OF RICE SOILS FROM THE TIDAL FLAT AREAS OF MUSI BANYUASIN, SOUTH SUMATRA B.H. Prasetyo; S. Suping; Subagyo H.; Mujiono Mujiono; H. Suhardjo
Indonesian Journal of Agricultural Science Vol 2, No 1 (2001): April 2001
Publisher : Indonesian Agency for Agricultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/ijas.v2n1.2001.p10-26

Abstract

Tidal flats in the Musi Banyuasin region that cover more than 200,000 ha are the largest area for agricultural development in South Sumatra Province. Only about a half of this has been used for tidal swamp rice fields, therefore, the other half needs to be developed. To obtain a better understanding of their properties for appropriate soil management, soil characteristics of the area need to be studied. To characterize the soil, thirty-four soil samples from seven soil profiles were analyzed for their chemical and mineralogical composition at the laboratories of the Center for Soil and Agroclimate Research and Development. The results indicate that soils from the tidal flat areas have an aquic soil moisture regime, the upper parts of the soils are mostly ripe, and most of the pedons show the presence of sulfidic materials below 65 cm of the mineral soil surface. The soils are classified as Sulfic Endoaquept (P1, P2), Histic Sulfaquent (P3), Typic Sulfaquept (P4), Fluvaquentic Endoaquept (P5), and Sulfic Hydraquent (P6, P7). Mineral composition of the sand fraction is dominated by quartz, while the clay minerals consist of predominantly kaolinite, mixed with small amount of smectite, illite, quartz, and crystoballite. Organic carbon content is high to very high, potential phosphate content of most pedons ranges from very low to medium, while potential potassium content varies from very low to medium in the upper layers and medium to very high in the bottom layers. Phosphate retention of topsoil sample varies from 56 to 97%, and is positively correlated (r2 = 0.73) with aluminum from amorphous materials. Exchangeable cations are dominated by Mg cation, and in all pedons cation exchange capacity values are medium to very high, and seem to be influenced by organic carbon. Specific chemical properties, particularly soil pH and content of exchangeable aluminum exhibit a significant change about 1-2 months after soil samples were taken from the field. Theoretically, interaction between good water management and fertilizer application are among the choices of management to make these soils productive.
EX VITRO ROOTING OF OIL PALM (Elaeis guineensis Jacq.) PLANTLETS DERIVED FROM TISSUE CULTURE Sumaryono Sumaryono; Imron Riyadi
Indonesian Journal of Agricultural Science Vol 12, No 2 (2011): October 2011
Publisher : Indonesian Agency for Agricultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/ijas.v12n2.2011.p57-62

Abstract

Plantlets of oil palm (Elaeis guineensis Jacq.) derived from so-matic embryos sometimes do not form well developed-roots. Root formation of unrooted-plantlets can be induced with aux-in during ex vitro acclimatization period to simplify the proce-dure and to reduce seedling production cost. Experiments were conducted using a completely randomized design to determine the effect of different types of auxin, i.e. indole-3-acetic acid (IAA), indole-3-butyric acid (IBA), and 1-naphthalene-acetic acid (NAA) at different concentrations, i.e. 0, 2, 4, 8, and 16 mM on root development of oil palm plantlets. The plantlets used were derived from somatic embryos of MK 649 oil palm clone. The basal end of the shoots was dipped in auxin solution for 10 minutes before the shoot was cultured in a small plastic pot containing a mixed growing medium. The cultures were then placed inside a closed transparent plastic tunnel (240 cm x 100 cm x 95 cm) for 12 weeks. The results showed that without auxin treatment only 15% of the shoots formed roots. Dipping in auxin solution increased significantly root frequen-cy to more than 50%. The best root formation was found on the shoots treated with 2 mM NAA by which rooting frequency was 80%. Auxin treatments also increased root quality as indi-cated by more number of primary and secondary roots. IAA, IBA, and NAA treatments at all concentrations tested increased significantly shoot height on average by 42% and shoot diame-ter by 30% compared to control treatment, but did not influ-ence root length. The best treatment for inducing roots of oil palm plantlets ex vitro was by dipping the basal end of the plant-lets in 2 mM NAA solution. The result showed that rooting of oil palm plantlets could be successfully conducted ex vitro that would eliminate sterile rooting stage thus simplify the protocol and reduce seedling production time and cost.
ROLE OF POLYAMINES IN INHIBITION OF ETHYLENE BIOSYNTHESIS AND THEIR EFFECTS ON RICE ANTHER CULTURE DEVELOPMENT Iswari S. Dewi; Bambang S. Purwoko
Indonesian Journal of Agricultural Science Vol 9, No 2 (2008): October 2008
Publisher : Indonesian Agency for Agricultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/ijas.v9n2.2008.p60-67

Abstract

The polyamines such as putrescine, spermidine, and spermine were reported to increase green plant regeneration in rice anther culture. Low response of anther culture of rice sub-species indica may be improved with the addition of putrescine in the culture media. Four experiments were conducted to study the role of polyamines in inhibition of ethylene biosynthesis and their effects on rice anther culture development. Anthers of two subspecies of rice, indica (IR64, Krowal, Jatiluhur) and japonica (Taipei 309) were cultured onto media supplemented with putrescine (N6P) and without putrescine (N6). Youngpanicles containing the anthers at mid-to-late nucleate microspores were cold pretreated at 5 + 2°C and incubated in the dark for 8 days before the anthers were cultured. Resultsshowed that medium without putrescine produced an earlier senescence of indica rice anther than that of japonica. The addition of 10-3 M putrescine into the culture media inhibited ethylene biosynthesis as anther senescence delayed, increased the three polyamines contents, and decreased the ACC content as well as ACC oxydase activity in anther-derived calli. In the anther and anther-derived calli of subspecies indica, the totalpolyamines content was lower (10.14 nM g-1 anther and 8.48 nM g-1 calli) than that of subspecies japonica (12.61 nM g-1 anther and 10.16 nM g-1 calli), whereas the ethylene production was higher (32.31 nM g-1 anther and 2.48 nM g-1 calli) than the japonica (31.68 nM g-1 anther and 1.76 nM g-1 calli). This study suggests that application of 10-3 M putrescine in anther culture of rice subspecies indica improves androgenesis by inhibitingearly senescence of cultured anthers and enhancing embryo or callus formation from microspores.
Front-matter IJAS 180217 Slamet Sutriswanto
Indonesian Journal of Agricultural Science Vol 18, No 2 (2017): December 2017
Publisher : Indonesian Agency for Agricultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/ijas.v18n2.2017.p%p

Abstract

CHARACTERISTICS OF PHOSPHATE ROCK MATERIALS FROM CHINA, INDONESIA AND TUNISIA AND THEIR DISSOLUTION IN INDONESIAN ACID SOILS Yusdar Hilman; Mohamed Hanafi Musa; Anuar Abdul Rahim; Azizah Hashim; Justina Sri Adiningsih
Indonesian Journal of Agricultural Science Vol 7, No 2 (2006): October 2006
Publisher : Indonesian Agency for Agricultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/ijas.v7n2.2006.p43-50

Abstract

Dissolution of phosphate rock (PR) in soils is a primary concern for P in the PR to be available for plant. The dissolution of three PR materials, China (CPR), Ciamis (IPR) and Gafsa (GPR), in eight acid Indonesian soils (pH in water 4.1-5.7) was tested in a closed incubation system. Experiment was conducted in Soil Chemical Laboratory, Universiti Putra Malaysia and Indonesian Center for Agricultural Land Resources Research and Development from January to April 2002. The dissolution was determined from the increase in either 0.5 M NaOH extractable P (∆P) or 1 M BaCl2-triethanolamine (TEA)-extractable Ca (∆Ca) in soils amended with PR compared with control soil. Dissolution of the IPR was the highest (30-100%) followed by GPR (17-69%) and then by CPR (20-54%). The maximum dissolution followed the order: Bogor Ultisols > Bogor Oxisols > Subang Inceptisols > Bogor Inceptisols > Sukabumi Oxisols > Lebak Ultisols > Sukabumi Inceptisols > Lampung Ultisols. PR dissolution indicated a positive correlation with P retention capacity. The results implied that the extent of PR dissolution for the three PR sources (China, Indonesia and Tunisia) increased with increasing P retention capacity of the soils. PR dissolution can be based on a calibration curve of ∆Ca meaning that if ∆P is high then the amount of PR dissolution measured by ∆Ca in PR materials is also high.
EFFECT OF PERMEABLE VESSEL CLOSURE AND GELLING AGENT ON REDUCTION OF HYPERHYDRICITY IN IN VITRO CULTURE OF CARNATION B. Winarto; M.A. Aziz; A.A. Rashid; M.R. Ismail
Indonesian Journal of Agricultural Science Vol 5, No 1 (2004): April 2004
Publisher : Indonesian Agency for Agricultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/ijas.v5n1.2004.p11-19

Abstract

Hyperhydricity, an abnormal morphological appearance and physiologicalfunction, is an important problem in carnation tissue culture. The problem causes premature flowering, high occurrence of abnormal shoots, difficulty in transferring hyperhydric plantlets to soil, and low survival rate of plantlets. High relative humidity and the water potential are considered as the key factors involved in the abnormality. Furthermore, permeable culture vessel and gelling agent were assured to be high potential treatment to eliminate it. Objective of this research was to reduce  hyperhydricity in regenerants of carnation using different permeable vessel closures and gelling agents and to assess the multiplication and  acclimatization abilities of recovered shoots. Experiment was arranged in randomized complete block design with four replications. First factor was different types of closure, i.e. cotton wool, plastic wrap, parafilm and aluminium foil, while second one was gelling agents, i.e. bacto agar, phytagel, swallow agar, and Type 900 agar. The recovered shoots were then multiplied, rooted, and acclimatized. The results showed that hyperhydricity was successfully reduced by applying permeable closure (cotton wool and plastic wrap) in combination with Type 900 agar. The combination of plastic wrap and Type 900 agar was the most appropriate treatment in reducing hyperhydricity and producing good quality shoots. The treatment reduced the problem down to 23% of total condition of hyperhydricity (100%) and increased leaf chlorophyll content from 0.0883 to 0.1288 mg mg-1. The plastic wrap was easily applied and cheaper material compared to cotton wool. The recovered shoots were able to produce 1-3 healthy axillary shoots and easily rooted on half-strength MS. The recovered plantlets were simply acclimatized with survival rate up to 100% on kossas peat + soil (1:1, v/v) and flowered 4-5 months after acclimatization with decreasing in number and size of flower.
FARMERS WILLINGNESS TO ACCEPT (WTA) FOR SUBMERGENCE RICE VARIETIES AT FLASH FLOOD AND FLOOD PRONE AFFECTED RICE AREA Made Oka A. Manikmas
Indonesian Journal of Agricultural Science Vol 13, No 2 (2012): October 2012
Publisher : Indonesian Agency for Agricultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/ijas.v13n2.2012.p68-79

Abstract

Farmers are rational in decision making process with respect to any introduced agricultural technology. Farmers may consider the economic sacrifices in term of additional cost and potential benefit or additional income before they accept and adopt the introduced technology. This study aimed to analyze farmer’s criteria and determine explanatory variables affecting farmer’s decision to accept or to adopt submergence tolerant (Sub-1) rice varieties at flash flood and flood prone affected rice area. The study was conducted in Indramayu District, West Java, and Kayu Agung District, South Sumatra. Contingent Valuation Method (CVM) that derived Willingness to Accept (WTA) approach was exercised to analyze explanatory variables that influence farmers’ willingness to accept introduced rice varieties. The results showed that the economic cost of flooding that damaged rice was about US$7.63 million in Kayu Agung and US$11.25 million in Indramayu in every wet season planting. Farmer’s criteria used in submergence tolerant varietal evaluation varied and location specific in nature. Most of explanatory variables used in the model were significantly influenced farmers’ WTA for submergence tolerant rice varieties such as: (1) availability of seed, (2) submergence tolerant for more than 14 days, (3) high yield, (4) proffer rice taste, (5) households’ income during normal year, (6) area planted during normal year, and (7) age of farm household head. The only indicator that did not significantly influence the farmers’ WTA for the Sub-1 rice varieties was farm household income during the flood year cropping.Abstrak Bahasa IndonesiaPetani cukup rasional dalam mengambil suatu keputusan yang terkait dengan teknologi introduksi. Petani selalu mempertim-bangkan korbanan ekonomi yang mereka keluarkan sebagai konsekuensi tambahan biaya dan potensi tambahan pendapatan sebelum mereka menerima dan mengadopsi suatu teknologi introduksi. Studi ini dimaksudkan untuk menganalisis faktor yang memengaruhi keinginan petani untuk menerima atau meng-adopsi varietas padi tahan rendaman (Sub-1) pada lahan yang mengalami banjir musiman atau lahan rawa lebak. Penelitian dilaksanakan di Kabupaten Indramayu, Jawa Barat, dan Kayu Agung, Sumatera Selatan. Contingent Valuation Method (CVM) yang menggiring keinginan petani untuk menerima atau menolak suatu teknologi introduksi, diterapkan untuk menganalisis peubah penjelas yang memengaruhi keinginan petani untuk menerima atau menolak padi tahan rendaman. Hasil penelitian menunjuk-kan bahwa biaya ekonomi yang ditimbulkan oleh kedua jenis rendaman pada lahan sawah sekitar US$7,63 juta di Kayu Agung dan US$11,25 juta di Indramayu pada setiap tanam padi di musim hujan. Kriteria yang digunakan petani dalam mengevaluasi varietas padi tahan rendaman bervariasi dan bersifat spesifik lokasi. Sebagian besar peubah penjelas yang digunakan dalam model secara nyata dan positif mampu menjelaskan keinginan petani untuk menerima dan mengadopsi varietas padi tahan rendaman, seperti: (1) ketersediaan benih, (2) varietas padi toleran rendaman sampai 14 hari, (3) hasil tinggi, (4) rasa sesuai dengan selera konsumen, (5) pendapatan rumah tangga petani pada tahun normal (tidak banjir), (6) area tanam pada tahun normal, dan (7) umur kepala rumah tangga petani. Satu-satunya peubah bebas yang tidak berpengaruh nyata terhadap keinginan petani untuk mengadopsi varietas padi Sub-1 adalah pendapatan rumah tangga petani pada tahun munculnya banjir dan merendam lahan sawah.
DEVELOPMENT OF EST-SSR MARKERS TO ASSESS GENETIC DIVERSITY OF BROCCOLI AND ITS RELATED SPECIES Nur Kholilatul Izzah; Reflinur Reflinur; Tae-Jin Yang
Indonesian Journal of Agricultural Science Vol 17, No 1 (2016): April 2016
Publisher : Indonesian Agency for Agricultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/ijas.v17n1.2016.p17-26

Abstract

Development of Expressed Sequence Tag-Simple Sequence Repeat (EST-SSR) markers derived from public database is known to be more efficient, faster and low cost. The objective of this study was to generate a new set of EST-SSR markers for broccoli and its related species and their usefulness for assessing their genetic diversity. A total of 202 Brassica oleracea ESTs were retrieved from NCBI and then assembled into 172 unigenes by means of CAP3 program. Identification of SSRs was carried out using web-based tool, RepeatMasker software. Afterwards, EST-SSR markers were developed using Primer3 program. Among the identified SSRs, trinucleotide repeats were the most common repeat types, which accounted for about 50%. A total of eight primer pairs were successfully designed and yielded amplification products. Among them, five markers were polymorphic and displayed a total of 30 alleles with an average number of six alleles per locus. The polymorphic markers were subsequently used for analyzing genetic diversity of 36 B. oleracea cultivars including 22 broccoli, five cauliflower and nine kohlrabi cultivars based on genetic similarity matrix as implemented in NTSYS program. At similarity coefficient of 61%, a UPGMA clustering dendrogram effectively separated 36 genotypes into three main groups, where 30 out of 36 genotypes were clearly discriminated. The result obtained in the present study would help breeders in selecting parental lines for crossing. Moreover, the novel EST-SSR markers developed in the study could be a valuable tool for differentiating cultivars of broccoli and related species.
NUTRITIONAL AND ROTI QUALITY OF SORGHUM GENOTYPES U.D. Chavan; J.V. Patil; M.S. Shinde
Indonesian Journal of Agricultural Science Vol 10, No 2 (2009): October 2009
Publisher : Indonesian Agency for Agricultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/ijas.v10n2.2009.p80-87

Abstract

Sorghum grains are mostly used for the roti preparation. The M 35-1 (Maldhandi) a sorghum cultivar is known for its good quality of roti due to having pearly white grain color, its flour having higher water holding capacity, and good organoleptic taste. However, this cultivar is low yielder. To evolve sorghum high yielding genotype coupled with this good roti qualities, systematic breeding program was planned and executed to overcome this problem. Twenty local land races, 92 genotypes, and seven improved cultivars of sorghum along with check M 35-1 were studied for various nutritional quality parameters, with special reference to the roti quality. Considering nutritional quality and organoleptic evaluation parameters studied for roti quality, the local land races viz., RSLG 428-1, RSLG 1238, RSLG 1275 and the genotypes viz., RSV 290, RSV 292, RSV 858, RSV 859, RSV 861, RSV 868, RSV 894, RSV 985, RSV 992, RSV 995, RSV 999 were found to be promising for protein, sugar, water absorption, and soluble protein content. Therefore, they can be used for further improvement in nutritional quality through breeding program. Among the improved cultivars, Phule Vasudha (RSV 423), CSV 22, and Phule Chitra (SPV 1546) werefound to be most promising for roti quality. The maximum score for overall acceptability was found for Phule Vasudha followed by Phule Anuradha (RSV 458), Phule Yeshoda (RSLG 262), and Phule Maulee (CSV 216).

Page 22 of 36 | Total Record : 352

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