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Buletin Plasma Nutfah
Published by Kementerian Pertanian
ISSN : 14104377     EISSN : 25491393     DOI : -
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology
Buletin Plasma Nutfah (BPN) is an open access scientific journal published by The Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development (ICABIOGRAD), The Indonesian Agency for Agricultural Research and Development (IAARD), Ministry of Agriculture. This peer-refereed journal covering the area of genetic resources including exploration, characterization, evaluation, conservation, diversity, traditional knowledge, management and policy / regulation, of all kinds of genetic resources: plants, animals, fishes, insects and microbes. Manuscripts submitted to this journal are those that have never been published in other journals. This journal is published in one volume of two issues per year (June and December). We invite authors to submit the manuscripts to this journal in English or Indonesian. Detail information about the journal, including author guidelines and manuscript template, is available on the website (http://ejurnal.litbang.pertanian.go.id/index.php/bpn). The manuscript should be submitted electronically through our submission system. Buletin Plasma Nutfah (BPN) is an open access scientific journal published by Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development (ICABIOGRAD), Indonesian Agency for Agricultural Research and Development (IAARD), Ministry of Agriculture. BPN Accredited by Ministry of Research, Technology and Higher Education of the Republic of Indonesia (No. 21/E/KPTP/2018) for period of 2016-2020. This peer-refereed journal covers the area of genetic resources including exploration, characterization, evaluation, conservation, diversity, traditional knowledge, management and policy / regulation related to genetic resources: plants, animals, fishes, insects and microbes which has never been published in other Journal
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Articles 6 Documents
 1 
Search results for , issue "Vol 19, No 1 (2013): June" : 6 Documents clear
Karakterisasi Fisiologi Cendawan Entomopatogen Lecanicillium lecanii sebagai Calon Bahan Aktif Bioinsektisida untuk Pengendalian Telur Kepik Coklat (Riptortus linearis) pada Kedelai Yusmani Prayogo
Buletin Plasma Nutfah Vol 19, No 1 (2013): June
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/blpn.v19n1.2013.p%p

Abstract

The aimof the research was to determine the physiological charactersof various L. lecanii isolates as active materials ofbioinsecticide to control pod sucking bug Riptortus linearisegg on soybean. Thirty seven (37) of L. lecanii isolates werecollected from four locations of soybean plantation inIndonesia. This study obtained four virulent isolates that werepotential as active ingredient of bioinsecticide i.e. Ll-JTM11,Ll-JTM12, Ll-JTM15, and Ll-TB2. Virulent isolates wereobtained from insect cadaver isolation in the field, while lessvirulent isolates were gained from soil. Physiologicalcharacters of potential isolates were fast colonization rate ofthe egg, thick and wholly, high sporulation with large conidialsize, high germination rate after 12 hours incubated in thewater, up to 95% germ tubes were formed. Clustering ofisolates based on the physiology character can determine thefungus virulence, while the grouping based on the source orhost location can not select fungal virulence. The virulentisolates had similarity in physiological characters equal to98%. Therefore, four potential isolates could be used asbiological agents in integrated pest mangement program(IPM), especially pod sucking bug R. linearis on soybean.
Peluang Perbaikan Varietas Lokal Padi Gogo Pasaman Barat Syahrul Zen; Abdul Aziz Syarif
Buletin Plasma Nutfah Vol 19, No 1 (2013): June
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/blpn.v19n1.2013.p%p

Abstract

Local varietyis the major component in upland rice cultivation as well asbreeding material in variety improve-ment. Local upland ricevarieties have been adapted to specific location, preferred andadopted by farmers although they are late in maturity. Thesereasons make it majority of farmers in West Pasaman District(West Sumatra) still cultivate local upland rice varieties. Toknow the yield potential and agronomic characters of widelycultivated of upland rice in West Pasaman District, a varietytrial conducted in 2010. Five local varieties and one improvevariety planted a Randomized complete block arrangementwith three replications. Observa-tion conducted on agronomiccharacters and yield and yield components. The results showedthat local variteies were tall (153-171 cm) and late maturity(130-157 days). The local varieties also showed higher numberof spikelet/panicle (249-454) lighter 1.000 grains weight (<20g) and higher yield than improved variety (4.27-5.51 comparedto 2.85 t/ha). In general all plant characters showed highheritability estimate (except number of productive tiller/hillr).High estimated of genetic variation coefficient and geneticadvance were shown on number of grain per panicle. Based onthese genetic parameters, selection at early generation for highyield can conducted on number of grain per panicle.
Karakteristik Karkas, Sifat Fisik dan Kimia Daging Rusa Sambar (Rusa unicolor) Bram Brahmantyo; Wirdateti Wirdateti; Gono Semiadi
Buletin Plasma Nutfah Vol 19, No 1 (2013): June
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/blpn.v19n1.2013.p%p

Abstract

Venison isknown as qualified meat because of its softness and fineness offiber, protein and iron are very high, as well as fat, cholesteroland energy are low. Today, consumers are likely to eat meatwith low fat. Venison will be able to meet the needs of themarket. This study aimed to evaluate carcass production,physical and chemical properties of venison of captive Sambardeer (Rusa unicolor). Three heads of adult Sambar deer fromthe Regional Technical Implementation Unit Breeding andArtificial Insemination Center, East Kalimantan were used.Deers were fasted for 24 hours and slaughter with cuttingjugular veins, esophagus and throat. Observations were madeon the carcass, commercial cuts, physical properties andchemical properties of venison. Averages of carcasspercentage, meat to bone ratio, and major commercial cutswere 50.17, 3.35, and 56.01%, respectively. The physicalproperties of the venison was very good and was notinfluenced by commercial cuts. Loin were higher on fatcontent (0.65+0.18 g/100 g) and cholesterol (48.33+11.59mg/kg) than the fillet and leg cuts.
In Vitro Medium-Term Conservation of Several Carnation Accessions through Nutrient Modification Kurniawan Budiarto; Budi Marwoto; Rudy Soehendi
Buletin Plasma Nutfah Vol 19, No 1 (2013): June
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/blpn.v19n1.2013.p%p

Abstract

Modification of osmotic pressure of in vitro medium-term canbe applied to conserve carnation accessions. This successfulmethod inspired the use of nutrient modification technique tohave the same function as far as these concerned. The researchwas then, conducted to evaluate in vitro media for preservingseveral carnation accessions in low temperature conditions.The research was carried out at Indonesian Ornamental CropsResearch Institute (IOCRI) from January 2007 to March 2008.A randomized completely block design with 25 replicationswas used to accomplish the combination of two factors. Thefirst factor was six commercial carnation accessions, namelycv. Light Pink Candy, Aicardy, Torpedo, Malaga, President,and Rendezvouz. While the second factor dealt with fourformulations of conservation media i.e. ½ MS + DMSO 3% +4% sucrose, modified hyponex + 4% sucrose, modifiedhyponex (no sucrose) and ½ MS + 4% sucrose (control). Theresults showed that medium term in vitro conservation forcarnation were successfully conducted up to 10 months usingin ½ MS + DMSO 3% + 4% sucrose and modified hyponex +4% sucrose without any significant differences and decreasesin viability when transferred in to shoot induction media. In theabsent of sucrose, however, the plantlet survivals were only upto 8 months. No significant variation on viability and numberof survival plantlet was observed among the carnationaccessions in all media tested.
Karakterisasi dan Identifikasi Isolat Bakteri Endofitik Penghambat Jamur Patogen Padi Yadi Suryadi; Tri P. Priyatno; I Made Samudra; Dwi N. Susilowati; Patricia Patricia; Wahyu Irawati
Buletin Plasma Nutfah Vol 19, No 1 (2013): June
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/blpn.v19n1.2013.p%p

Abstract

Disease caused byfungal pathogens often causing damage on rice crop. Thisstudy was aimed to characterize 10 endohytic bacterial isolatesin suppression of rice pathogenic fungi. Characterization by invitro test showed several endophytic isolates effective againstfungal pathogen Rhizoctonia solani (Rs) and Pyriculariaoryzae (Po). The bacterial culture filtrates could inhibit radialgrowth of fungal colonies with the Rs ranged percentageinhibition of 32.9-99.4%, whilst inhibition against Po wereranged from 3-98.2%, respectively. Based on chitinase assay,it was indicated that gram negative bacteria of E 76 isolateproduced clear zone and highest chitinolytic index. Theanalysis to the base sequence (total 1,322 bp) using 16s rRNAgene sequencing revealed that E76 isolates had 99% similaritywith Burkholderia sp.
Evaluasi Toleransi Plasma Nutfah Padi Lokal pada Lahan Masam Kahat Fosfor Tintin Suhartini; Dwinita W. Utami; Ida Hanarida
Buletin Plasma Nutfah Vol 19, No 1 (2013): June
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/blpn.v19n1.2013.p%p

Abstract

Phosporus (P) deficiency is one oflimiting factor for rice growth. In Indonesia P deficiency muchoccurs in acid soils. The use of P deficiency tolerant varietiesis the best solution compared to the application of P fertilizerdue to more efficient in cost. The purpose of this study was toevaluate the local rice germplasm collected from severalregions in Indonesia to P deficiency in acid soil. The study wasconducted in Jasinga West Java during wet season of 2006-2007 with the soil condition lack of P. The experiments wereconducted with the two treatments: first, without P fertilizerand second with P fertilizer equivalent of 25 kg P/ha. The total100 accessions of rice germplasm were screened in this fieldbased on a randomized block design with three replications.Fertilizing were given on experiments I and II with thecomposition: urea 300 kg/ha and 100 kg KCl. The field designwas done by plotting size is 1 x 5 m2, spacing plant of 25 cm x25 cm, and planting two seeds per hole. The yield componentscharacters which were observed: number of tillers, plant dryweight, plant height and flowering. The results of combinedanalysis showed that there is a significant interaction betweenP and genotype on the tiller number, whereas the dry weight ofplant, plant height, and flowering were is not significant. Theevaluation of 100 local rice genotypes to P deficiency byindicators of the tiller number and dry weight of plantsobtained 19 genotypes that are tolerant to P deficiency with therelative value of tillers number and dry weight of plants morethan 80%. Local varieties Mandalet, Ganefo, Padi Belanda,Pulut Jangan, Padi Ubek Bala, and Padi Krayan were the mosttolerant to conditions without P which showed the tillersnumber more 1-21% rather than the conditions with Pfertilizer. There are three selected genotypes, Pulut Jangan,Padi Ubek Bala, and Padi Krayan which increased the numberof tillers and dry weight of plants in without P conditions. Theincreasing of tillers number were reach 1-17% while the dryweight increased 12-41%.

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