cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
-
Contact Email
-
Phone
-
Journal Mail Official
-
Editorial Address
-
Location
Kota yogyakarta,
Daerah istimewa yogyakarta
INDONESIA
Indonesian Journal of Cancer Chemoprevention
Published by Indonesian Society for Cancer Chemoprevention
ISSN : 23558989     EISSN : 20880197     DOI : -
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology
Indonesian Journal of Cancer Chemoprevention (IJCC) is an open access, peer-reviewed, triannual journal devoted to publishing articles on Cancer Chemoprevention including Experimental and Clinical Pharmacology, especially concerning Anti-Oxidants, Anti-Aging, Anti-Inflammation, Anti-Angiogenesis, and Anti-Carcinogenesis; Cancer Detection; Stem Cell Biology; Immunology; in vitro and in silico Exploration of Chemopreventive Mechanism; and Natural Products.
Arjuna Subject : -
Articles 334 Documents
 4   5   6   7   8 
Combination of Doxorubicin and Areca Ethanolic Extract Induces Apoptosis by Increasing Caspase-3 Level on Breast Cancer (T47D) Cells Rahmi, Fitria; Meiyanto, Edy; Susidarti, Ratna Asmah
Indonesian Journal of Cancer Chemoprevention Vol 3, No 1 (2012)
Publisher : Indonesian Research Gateway

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4.399 KB)

Abstract

Despite causing many side effects, doxorubicin (Dox) is still one of breast cancer drug of choice. Thus, combination of chemotherapy is developed in order to decrease doxorubicin regimen dose. The aim of this research is to examine the combination effect of doxorubicin (dox) and areca extract (AE) on T47D human breast cancer cells. The cytotoxic activity was determined using MTT assay. The combination index (Cl) of the combination treatment was calculated to determine the effects (synergistic, additive or antagonistic). The combination application of dox (6-22nM) and AE (8-30μg/ml) on T47D cells showed synergistic (Cl<0.9) or addictive effect )Cl =0.9-I.I). The effective combincation of dox-AE was 6nM-8μg/ml on Cl<0.5. Apoptosis induction of AE solely and its combination with dox was the observed using double staining method. Moreover, expression of Bax and caspase-3 protein which mediated apoptosis, were observed using immunocytochemistry. Combination of AE and Dox increased expression of Caspase-3 but did not increase expression of Bax. This result showed AE increase the effectiveness of doxorubicin against T47D cells.Keyword : Breast cancer, doxorubicin, areca extract, T47D cells
The Safety of Areca Seed Ethanolic Extract as Potential Chemopreventive Agent is Proven by Acute Toxicity Test Handayani, Sri; Jenie, Riris Istighfari; Susidarti, Ratna Asmah
Indonesian Journal of Cancer Chemoprevention Vol 3, No 1 (2012)
Publisher : Indonesian Research Gateway

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4.399 KB)

Abstract

Areca (Areca catechu L.) seeds ethanolic extract (AE) exhibits antiproliferative activity and induces apoptosis on T47D and MCF-7 cells. This study aimed to verify AE safety using acute toxicity test to support its development as chemopreventive agent. Male Sprague Dawley Rat 9Rattus norvegicus) age 8 weeks divided into five groups, one group of control treated with 0.5 % CMC-Na only and four groups for treatment. Single dose in oral administration was done to test animal with various dose of AE starts from lowest dose to highest dose expected toxic to all of test animal (0.1; 0.72; 5.36 and 10 gram/kgBW). Observation was done during 24 hours and continued for 14 days. The observation criteria were toxic symptoms, appearance and mechanism of toxic effect and pathology of vital organ. Histopathology analysis of some vital organs was done with Haematoxyllin & Eosin (H&E) staining. Toxic effect did not appear either on treatment groups or control group. Treatment of single dose of areca ethanolic extract, even in highest dose, did not cause the death of the animals. Therefore, observation extended to 14 days and terminated by necroption of the animals. All of group did not show histopathological alterations in microscopic observation. Category of the potential toxicity of AE is practically non-toxic, ie 10 g/kgBW. The result show the safety of areca seed ethanolic extract which is important for its development as chemopreventive agent.Key words: Areca catechu, acute toxicity, rat
Anti-Proliferative Activity of Nigella sativa Chloroform Extract on 7,12-Dimenthylbenz[a]anthracene Induced Female Rats Splenocyte Firdaus, Ahmad Fiki; Sobri, Iskandar; Ekowati, Heny
Indonesian Journal of Cancer Chemoprevention Vol 3, No 1 (2012)
Publisher : Indonesian Research Gateway

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4.399 KB)

Abstract

Previous study reported that Nigella sativa has in vitro and in vivo cancer activity. This study was conducted to observe the effect of chloroform extract of Nigell sativa seed (NCE) on 7,12-Dimethylbenz[a]anthracene (DMBA)-induced female rats splenocyte. The experiment consisted of five groups, corn oil solvent control group, DMBA group, DMBA+250 mg/kgBW NCE, DMBA+500 mg/kgBW NCE and DMBA+750 mg/kgBW NCE. Extract was dissolved in corn oil and oral administered daily for 2 weeks before and during the DMBA induction. Observation of cell proliferation was performed using haematoxylin and eosin (H&E) and AgNOR stainings. H&E staining showed decreased necrocis activity extract groups compared to DMBA group. From AgNOR staining results, mean AgNOR (mAgNOR) of extract groups was less in number compared to DMBA group. The mAgNOR in corn oil solvent control group, DMBA group, DMBA+250 mg/kgBW NCE, DMBA+500 mg/kgBW NCE and DMBA+750 mg/kgBW NCE were 1.22, 1.91, 1.29, 1.36 and 1.33, respectively. Our current results showed that NCE reduces the proliferation of DMBA-induced rat spleenocytes. Thus, NCE has potency to be developed as a chemopreventive agent.Keywords : Nigella sativa, spleen, DMBA, anti-proliferative
Antiproliferative Effect of Chloroform Extract of Cangkring Leaves (Erythrina fusca Lour.) and Its Isolates against Hela Cells Puspitasari, Endah; Setyowati, Endah Dwi; Dhiani, Binar Asrining; Riyanto, Sugeng
Indonesian Journal of Cancer Chemoprevention Vol 3, No 1 (2012)
Publisher : Indonesian Research Gateway

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4.399 KB)

Abstract

Cervical cancer is a type of cancer possessing the 3rd highest incidence among women worldwide. Todays therapies against cancer are still ineffective, thus people are seeking for alternative method. Erythrina fusca Lour. has been used traditionally as anticancer. This experiment is conducted to study the ability of chloroform extract of E.fusca Lour. leaves and its isolates on HeLa cervical cancer cells. The cytotoxicity assay and doubling time assay were done using direct counting method, while the DNA staining assay was done using acrydine orange. The chloroform extract and isolate #30 showed cytotoxic activity with IC50 of 16 and 5 μg/ml, respectively, while isolate #2 and isolate #8 didnt. The effluent #30 could not affect HeLa cells growth at the given concentration, but it might promote DNA fragmentation indicating apoptosis induction. The molecular mechanisme underlying these effects still need to be further explored.Keywords :  Erythrina fusca Lour., isolate and effluent of chloroform extract, antiproliferative, cervical cancer
Combination of Tangeretin and 5-Fluorouracil Modulates Cell Cycle and Induce Apoptosis on Widr Cells Indriyani, Luthfia; Hermawan, Adam; Jenie, Riris Istighfari
Indonesian Journal of Cancer Chemoprevention Vol 3, No 1 (2012)
Publisher : Indonesian Research Gateway

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4.399 KB)

Abstract

Co-chemotherapeutics approaches are increasing in cancer treatment in order mainly to suppress the resistance phenomenon of cancer treatment and to enhance the cytotoxic effect of the main chemotherapeutics agent. Tangeretin has been known to have cytotoxic effect to some cancer cells through some pathways in the cells. To explore the potential effect of tangeretin as co-chemotherapeutics agent this research was subjected to study the cytotoxic  effect of tangeretin in combination with 5-Fluoro Uracil (5-FU) on WiDR colon cancer cells covering the modulation of cell cycle and apoptosis induction. Cytotoxic effect was examined by using MTT assay while apoptosis induction was determined by annexin-V flowcytometry. Under MTT assay, tangeretin showed weak cytotoxic activity on the cells. However, tangeretin significantly enhanced the cytotoxic effect of 5-FU on the cells. This co-chemotherapeutics effect likely correlated with cell cycle modulation effect, especially in inducing polyploidy phenomenon as expressed in the flowcytometric graph of the DNA content. This combination also increased apoptosis induction. These result suggest that tangeretin is potential to be developed as co-chemotherapeutic agent for 5-FU on colon cancer and further molecular mechanism need to be exploredKeywords : Tangeretin, 5-Fluorourasil, WiDr, cell cycle, apoptosis
Antiproliferative Effect of Ethanolic Extract Eugenia uniflora Lam. Leaves on T47D Cells Ismiyati, Nur; Putri, Dyaningtyas Dewi Pamungkas; Kusumastuti, Siska Andrina; Febriansyah, Rifki
Indonesian Journal of Cancer Chemoprevention Vol 3, No 2 (2012)
Publisher : Indonesian Research Gateway

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4.399 KB)

Abstract

Eugenia uniflora Lam. is one of herbal products developed for anticancer. The aim of the present study was to identify the antiproliferative effect of ethanolic extract of Eugenia Uniflora Lam. leaves (EEU) on breast cancer cell line T47D. This Research was initiated by extracting the active contents of Eugenia uniflora Lam. leaves by maceration with ethanol 96%. The extract was then analyzed by thin layer chromatography (TLC). Cytotoxic assay of EEU was carried out by using MTT assay. Apoptosis phenomenon was observed with double staining using acridine orange-ethidium bromide. EEU showed cytotoxic effct on T47D cells with IC50 value of 65 μg/ml. Moreover, EEU 50 μg/ml and 100 μg/ml induced apoptosis. TLC examination showed that EEU used in this study contain phenolic, flavonoid, and saponin compounds which were suggested to be responsible for antiproliferative effect. Further molecular mechanism underlying EEU antiproliferative effect needs to be done.Keywords : Eugenia uniflora Lam., T47D cells, antiproliferative, apoptosis
Secang (Caesalpinia sappan L.) Heartwood Ethanolic Extract Shows Activity as Doxorubicin Co-chemotherapeutic Agent by Apoptosis Induction on T47D Breast Cancer Cells Nurzijah, Ika; Putri, Dyaningtyas Dewi Pamungkas; Rivanti, Erlina; Meiyanto, Edy
Indonesian Journal of Cancer Chemoprevention Vol 3, No 2 (2012)
Publisher : Indonesian Research Gateway

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4.399 KB)

Abstract

Doxorubicin, primary chemoteurapeutic agent used for breast cancer treatment, is known to have various side effects included multi drug resistance 9MDR) phenomenon. Therefore, exploration of co-chemotherapeutic agent is important to be conducted in order to prevent MDR. Secang (Caesalpinia sappan L.) which contains active compounds brazilin and brazilein, is proven to have activity as anticancer. The aim of this study is to determine the potency of Caesalpinia sappan L.ethanolic extract (CEE) as co-chemotherapeutic agent of doxorubicin and its mechanism through apoptosis induction on T47D breast cancer cells. Caesalpinia sappan L. heartwood powder was macerated with ethanol 70%. The cytotoxic effect of CEE alone and its combination with doxorubicin was analyzed using MTT assay. Apoptosis assay was done by flowcytometry-annexin V method. CEE showed cytotoxic activity on T47D cells with IC50 value of 35 μg/ml, while combinatorial test showed that all of combination doses of CEE and doxorubicin gave synergistic effect. Flowcytometry-annexin V assay proved that treatment of CEE induced apoptosis of doxorubicin. Based on these results, we conclude that Caesalpinia sappan L. heartwood ethanolic extract is potential to be developed as co-chemotherapeutic agent of doxorubicin.Keywords : Caesalpinia sappan L., doxorubicib, apoptosis, T47D cells. 
Ethanolic Extract of Papaya (Carica papaya) Leaf Exhibits Estrogenic Effects In Vivo and In Silico Sugiyanto, Raisatun Nisa; Khamsita, Rahmi; Lambertus, Marvin; Utomo, Rohmad Yudi; Susidarti, Ratna Asmah
Indonesian Journal of Cancer Chemoprevention Vol 3, No 2 (2012)
Publisher : Indonesian Research Gateway

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4.399 KB)

Abstract

The menopause women have the low level of estrogen in the body. The lack of estrogen changes physiological function in womens body that affects in health condition. Carica papaya L. leaf contains flavonoid quercetin which exhibits estrogenic effect. The aim of this study is to determine the estrogenic effect of papaya leaves extract (PLE) in vivo, and in silico. Papaya leaves were extracted by ethanol 70% maceration. The in silico study were done by molecular docking between quersetin and Estrogen Receptor (ERα and ERß) to obtain the docking score. Based on this study, docking score of quercetin was almost similar to the native ligand of ER. The in vivo study was done as follow: 36 female rats Sprague Dawley divided into six groups. The groups are shame-ovariectomized (S-OVX), control ovariextomized (OVX), CMC-Na control (OVX+CMC-Na), positive control (OVX+Estradiol), and the PLE treatment groups dose 750 mg/kgBW (OVX+750mg/kgBW) and dose 1000 mg/kgBW (OVX+1000 mg/kgBW). Administrations of PLE were done in three weeks orally, while estradiol was administrated intraperitonially. The mammae and uterine were sliced for analysis. Based on the study, the treatment of PLE increased the number of mammae lobules and uterine weight as well as estrogen does. In summary, PLE can be developed as a source of phytoestrogens.Keywords: Carica papaya L., phytoestrogen, estrogen receptor, mammae lobule, uterine
Ethanolic Extract of Mangosteen (Garcinia mangostana) Peel Inhibits T47D and Hela Cells Line Proliferation Via Nf-kB Pathway Inhibition Rivanti, Erlina; Rohmah, Annishfia Lailatur; Putri, Herwandhani; Tirtanirmala, Prisnu; Pamungkas, Dyaningtyas Dewi Putri
Indonesian Journal of Cancer Chemoprevention Vol 3, No 2 (2012)
Publisher : Indonesian Research Gateway

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4.399 KB)

Abstract

Effective and selective chemoterapeutic and chemopreventive agent is needed to cure breast and cervical cancers. One of the potential natural material is mangosteen peel (Garcinia mangostana). In this study, we observed cytotoxic effect of ethanolic extract of mangosteen peel (EMP) on HeLa cells line and T47D cells line. The cytotoxic effect was determined using MTT assay. EMP showed cytotoxic effect on T47D cells and HeLa cells with IC50 values of 2.07 μg/ml and 10.58 μg/ml respectively. Molecular docking simulation was done to predict the molecular mechanism of active compund in mangosteen peel extract, α-mangostin, in NFκB pathway which is one of the potential pathway to induce cytotoxicity on T47D and HeLa cells. Docking was done using PLANTS software and the binding score between α-mangostin and proteasom is -78,12, whereas the binding score between α-mangostin and IKK is -86.84. These results showed the possiblity mechanism of mangostin peel extract containing α-mangostin inhibits IKK activation in NFκB pathway. Based on this study, we conclude that mangosteen peel extract is potential to be developed as chemopreventive agent toward cervical and breast cancers.Keywords : Mangosteen peel (Garcinia mangostana), cytotoxic, T47D cells, HeLa cells, NFκB
The Number of Macrophages and Heterophils on Chick Embryo Chorioallantoic Membrane After Gynura procumbens (Lour) Merr Extract Treatment and bFgF Induction Hamid, Iwan Sahrial; P, Yuseni Kusuma; Bijanti, Retno; Aksono, E Bimo
Indonesian Journal of Cancer Chemoprevention Vol 3, No 2 (2012)
Publisher : Indonesian Research Gateway

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4.399 KB)

Abstract

Antiangiogenesis (inhibition of new blood vessels formation) has become a strategy to inhibit cancer development. The aim of this experiment was to investigate antiangiogenic effect  of  Gynura  procumbens  (Lour)  Merr  focusing  on  the  decreasing  of  the  number  of macrophages  and  heterophils  on  chick  embryo  chorioallantoic  membrane.  Nine-days-aged-eggs  were  divided  into  six  groups  (eight  eggs  each  group).  Group  I  (positive  control)  eggs were  induced  with  bFGF+Tris  HCl.  Group  II  (negative  control)  eggs  were  treated  with DMSO+Tris  HCl.  Group  III  (treatment  I)  eggs  were  induced  with  60  ng  bFGF  and  treated with  ethanolic  extract  of  G.  procumbens  leaves  with  the  dose  of  60  µg.  The  following treatment  groups,  i.e.  group  IV  (treatment  II),  group  V  (treatment  III),  and  group  VI (treatment IV) were treated with increasing dose of extract, starting from 75 µg, 90 µg, and the last was 110 µg. Eggs were incubated until they reach the age of twelve days to observe macrophages, while  to observe heterophils, eggs were incubated until  the  age of seventeen days. Based on haematoxylin-eosin staining, macrophages in the treatment groups were less than the control positive group (bFGF+Tris HCl), but based on giemsa staining, the effect of Gynura procumbens  in decreasing the number of heterophils could not  be observed  because some blood smears. These analysis suggest that  the  ethanolic extract of  Gynura procumbens leaves can perform as antiangiogenic agent decreasing the number of macrophages.Keywords: antiangiogenic, macrophages, heterophils, Gynura procumbens

Page 6 of 34 | Total Record : 334

 4   5   6   7   8 

Filter by Year

2010 2024


Reset
Filter By Issues
All Issue Vol 15, No 2 (2024) Vol 15, No 1 (2024) Vol 14, No 3 (2023) Vol 14, No 2 (2023) Vol 14, No 1 (2023) Vol 13, No 3 (2022) Vol 13, No 2 (2022) Vol 13, No 1 (2022) Vol 12, No 3 (2021) Vol 12, No 2 (2021) Vol 12, No 1 (2021) Vol 11, No 3 (2020) Vol 11, No 2 (2020) Vol 11, No 1 (2020) Vol 10, No 3 (2019) Vol 10, No 2 (2019) Vol 10, No 1 (2019) Vol 9, No 3 (2018) Vol 9, No 2 (2018) Vol 9, No 1 (2018) Vol 8, No 3 (2017) Vol 8, No 2 (2017) Vol 8, No 1 (2017) Vol 7, No 3 (2016) Vol 7, No 2 (2016) Vol 7, No 1 (2016) Vol 6, No 3 (2015) Vol 6, No 2 (2015) Vol 6, No 1 (2015) Vol 4, No 1 (2013) Vol 3, No 3 (2012) Vol 3, No 3 (2012) Vol 3, No 2 (2012) Vol 3, No 2 (2012) Vol 3, No 1 (2012) Vol 3, No 1 (2012) Vol 2, No 3 (2011) Vol 2, No 3 (2011) Vol 2, No 2 (2011) Vol 2, No 2 (2011) Vol 2, No 1 (2011) Vol 2, No 1 (2011) Vol 1, No 2 (2010) Vol 1, No 2 (2010) Vol 1, No 1 (2010) Vol 1, No 1 (2010) More Issue