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INDONESIA
Jurnal Matematika & Sains
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Articles 339 Documents
PCR Tanpa Isolasi DNA dari Sel Epitel Rongga Mulut Ahmad Saifuddin Noer; Marsia Gustiananda
Jurnal Matematika & Sains Vol 2, No 1 (1997)
Publisher : Institut Teknologi Bandung

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Abstract

The polymerase chain reaction (PCR) has become a widely used technique for the amplification of DNA for gene cloning, polymorphisms detection, disease diagnostics, and many others. The starting material for PCR is amount of DNA consist of the sequence interested to be amplified, known as template DNA. Tempate DNA can be obtained in several ways. The preparation of template DNA from muscle tissue by biopsy, blood samples by veni puncture, follicle cells in single hairs, and the cells from urinary tract still have some disadvantages. Some of the methods are invasive, and some of them require DNA isolation steps. In the recent study, we report that preparation of template DNA can be done without isolation steps, and the starting material is mouth epithelial cells obtained from mouth wash fluid. This new method is conducted in the following steps : (i) collecting epithelial cells in mouth wash fluid, (ii) extraction of the cells, (iii) breaking the cells with lysis buffer, (iv) using lysat as a source of template DNA for PCR. Result of this simple procedure showed as 0,4 kb in size band of mitochondrial DNA fragment, that obtained from PCR employing primer M-1 and M-2. PCR without DNA isolation from mouth epithelial cells will be of benefit in the study of population analysis, forensics, and is the potential method for diagnostics.
Pemakaian Metode Numerik Pada Sirkulasi Udara di Sekitar Bangunan Tradisional Bali I Gusti Bagus Wijaya Kusuma
Jurnal Matematika & Sains Vol 7, No 1 (2002)
Publisher : Institut Teknologi Bandung

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Abstract

The airflow patterns around traditional Balinese buildings cannot be estimated by experiment method nor using modern buildings, since the position and the function of a building on a cluster of traditional Balinese buildings are linked each other. It is not correct if the investigation uses an isolated building and symmetrical method in order to understand the airflow patterns of all buildings. Numerical method based on finite volume is used in this simulation since it gives quicker analyses and capable of delivering more detailed and comprehensive information about the flow structure around 3-dimension buildings. Results show that numerical method gives more accurate prediction of airflow patterns around traditional buildings.
Profil Farmakokinetik dan Ketersediaan Hayati Tiga Sediaan Tablet Natrium Diklofenak Salut Enterik Yeyet Cahyati Sumirtapura; Herwanto Suhalim; Weda Wibawati
Jurnal Matematika & Sains Vol 2, No 2 (1997)
Publisher : Institut Teknologi Bandung

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Abstract

Pharmacokinetic and bioavailability studies of three enteric coated 50-mg sodium diclofenac tablets marketed in Indonesia (DR-50, FR-50 and VN-50) have been carried out in six healthy Indonesian volunteers with three-way crossover design. The results obtained showed that there were significant differences in the bioavailability parameters of the three products tested. The lag-time observed varied from 1 to 2 hours for DR-50, 0.5 to 2 hours for FR-50, and 1.5-2 hours for VN-50. Peak plasma levels of 1.16 + 0.59, 2.06 + 1.03, and 0.68 + 0.3 μg/ml were reached in 3.3 + 1.4, 1.9 + 0.9, and 3.8 + 1.2 hours following administration of DR-50, FR-50 and VN-50, respectively. The area under the curve given by each product were 3.81 + 1.60 μg/ml.hr for DR-50, 3.87 + 0.89 μg/ml.hr.for FR-50, and 3.13 + 1.33 μg/ml.hr for VN-50. The elimination half life of diclofenac observed varied from 2.1 to 6.9 hours with a mean value of 3.7 + 1.5 hours.
Antimicrobial Activities of the Ethanol Extracts of Capsicum Fruits with Different Levels of Pungency Soediro Soetarno; Sukrasno Sukrasno; Elin Yulinah; Sylvia Sylvia
Jurnal Matematika & Sains Vol 2, No 2 (1997)
Publisher : Institut Teknologi Bandung

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Abstract

Ethanol extracts of the fruits of three kinds of Capsicum showed similar potencies in their antimicrobial activities against Gram (+) and Gram (-) bacterias, and fungi, although they contained different level of capsaicin. Bioautographic tests demonstrated that capsaicin was the main antimicrobial component. At least two other non-polar components of ethanol extract also contributed in the antimicrobial activity and very likely that these compounds were responsible for the activity toward Pseudomonas aeruginosa.
Colloid Chemical Rheology of Non-Aqueous Advanced Electroceramic Slips Bambang Ari Wahjudi
Jurnal Matematika & Sains Vol 2, No 2 (1997)
Publisher : Institut Teknologi Bandung

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Abstract

In many, if not all ceramic industries it is becoming increasingly important to be able to understand and quantify processing behaviour of materials such that final product performance can be accurately determined. In the present work, the usefullness of the so called Stress-Controlled Rheometer in characterising the rheological properties of alumina slips is demonstrated. The alumina slips investigated exhibit more Newtonian character with predominating viscous component at low frequencies, on the other hand shifted to about 22% viscoelastic character at higher oscillating frequencies. Rheological behaviour reflects the extent of interparticulate interaction in a manner that polymer chains conformation is responsible for either the observed elastic nature or dissipative viscous character of flow. Further, it is also revealed that viscoelasticity in a ceramic slip is determined largely by the extent of polymer barrier interpenetration in the overlapped region.
Mechanistic Studies on Photoacylation Megawati Santoso
Jurnal Matematika & Sains Vol 2, No 2 (1997)
Publisher : Institut Teknologi Bandung

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Abstract

An unprecedented photoacylation method for preparation of acyl- and aroyliron(III) porphyrin complexes has been reported1). This paper present the mechanistic studies on such photoacylation. Detailed mechanistic studies on the photoacylation of chloroiron(III) tetraphenylporphyrin ((TPP)FeCl) suggest "controlled" radical reactions. In the presence of UV light (TPP)FeCl undergoes intramolecular electron transfer to yield (TPP)Fe(II) and chlorine radical. Aldehyde prevents the back electron transfer by trapping the chlorine radical to form the corresponding acyl radical. The photoreduced (TPP)Fe(II) then undergoes oxidative addition with the acyl radical to yield the corresponding acyliron(III) tetraphenylporphyrin complex.
Dinamika Gelombang Cnoidal di Atas Dasar Tak Rata Menggunakan Persamaan Gelombang Dua Arah Boussinesq Warsoma Djohan
Jurnal Matematika & Sains Vol 2, No 2 (1997)
Publisher : Institut Teknologi Bandung

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Abstract

Waves over uneven bottom which are running in two directions are governed by Boussinesq equations. When the bottom is flat, the Boussinesq equations have a periodic travelling wave solution, i.e. a solution which is running undisturbed in shape and velocity. This travelling wave is called a cnoidal wave. In this research the distortion of a cnoidal wave due to decreasing depth will be studied numerically. A cnoidal wave is initially running above flat bottom, then the depth decreases and flat again with depth h1 (< h0). First, a cnoidal wave is constructed as solution of the constrained critical point problem, i.e. finding the extremism of energy with constraints momentum and mass. Then the Boussinesq equations are discritized using the direct Fourier truncation method. Numerical simulation shows that the cnoidal wave constructed above is indeed a travelling wave solution. By choosing h1 to be the eigendepth of the two-soliton solution of KdV according to the inverse scattering theory, numerical simulations show the splitting process of an initial cnoidal wave into two waves.
Zonasi Daerah Pantai Berdasarkan Populasi Foraminifera Muda : Studi Kasus Teluk Banyuwangi Agus Supangat
Jurnal Matematika & Sains Vol 3, No 1 (1998)
Publisher : Institut Teknologi Bandung

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Abstract

The total population off forminifera found in Banyuwangi Bay is 53 spesies. It consists of 3 types : 64,4% hyaline type, 22,6% porcelaine type, and 13,2% agglotine type. The density ranges between 17000 - 38000 individu per 50 cm3 sediment. Based on Fisher diversity index this area is divided into three regions. Species that are predominantly found in Banyuwangi Bay are Ammonia tepida, Bolivina striatula, Haynesina germanica, Elphidium advenum, Quinqueloculina seminula, Reusella aculeata, Pararotalia sp and Miliammina spp. The results of the factorial analysis test based on station and its species show that there are three zonations in the Banyuwangi Bay. The first zone is influenced by the Java Sea, the second zone is influenced by shrimp pond wastes, and the third zone by the mangrove.
Temperature Dependent of The Forbidden Energy Gap of GaSb Grown by MOCVD Agus Subekti
Jurnal Matematika & Sains Vol 5, No 1 (2000)
Publisher : Institut Teknologi Bandung

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Abstract

This work reports on the investigation of the temperature dependent of the forbidden energy gap of GaSb. The GaSb samples were grown by MOCVD technique using trimethylgallium (TMGa) and trimethylantomony (TMSb) as the precursors. The temperature dependent of the energy gap was obtained from a plot of the square of absorption coefficient versus photon energy at temperature range of 10-300K follows the equation , wich in good agreement with results previously reported in the literatures.
Perhitungan Respons Elektromagnetik Lapisan Tipis Heterogen Menggunakan Metoda Persamaan Integral Hendra Grandis; Nanang T. Puspito
Jurnal Matematika & Sains Vol 3, No 1 (1998)
Publisher : Institut Teknologi Bandung

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Abstract

The computation of electromagnetic response of a 3-D model which represents subsurface electric conductivity distribution is done by resolving the Maxwells equations using the integral equation method. In order to simplify the problem, it is assumed that the 3-D inhomogeneity is confined in a thin layer embedded in a horizontally stratified (1-D) medium. The algorithm is applied to compute the response of two simple models containing either resistive and conductive inhomogeneity. The apparent resistivity, phase and magnetic transfer function give general features of the models.

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