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Jurnal Ilmu Ternak Veteriner

JITV
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Published by Indonesian Center for Animal Research and Development

ISSN : 08537380 EISSN : 2252696X DOI : -

Core Subject : Health,

Veterinary

Aims JITV (Jurnal Ilmu ternak dan Veteriner) or Indonesian Journal of Animal and Veterinary Sciences (IJAVS) aims to publish original research results and reviews on farm tropical animals such as cattle, buffaloes, sheep, goats, pigs, horses, poultry, as well as non domesticated Indonesian endemic animals, such as deers, anoa, babirusa, etc. Scope Indonesian Journal of Animal and Veterinary Sciences . The journal will consider primary research papers from any source if they make an original contribution to the experimental or theoretical understanding and application of theories and methodologies of some aspects of animal science and veterinary

Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Hewan dan Zoologi

Articles 9 Documents

Search results for , issue " Vol 18, No 3 (2013)" : 9 Documents clear

Screening test for detection of Newcastle Disease, Avian Influenza and Infectious Bronchitis viruses using multiplex reverse transcriptase polymerase chain reaction approach R, Hartawan; NLPI, DharmayantI
Indonesian Journal of Animal and Veterinary Sciences Vol 18, No 3 (2013)
Publisher : Indonesian Animal Sciences Society

Numerous viral pathogens are circulated in the environment of commercial chicken farms that causing the difficulty in the confirmation of diagnosis. A breakthrough on the diagnosis technique is required in order to deal with multiple viral infections. Ideally, the approach should have not only high accuracy but also economical and straightforward. The objective of this research is to develop a rapid multiplex reverse transcriptase polymerase chain reaction (mRT-PCR) diagnostic method for three common infectious viral diseases in poultry, Newcastle Disease (ND), Avian Influenza (AI) subtype H5N1 and Infectious Bronchitis (IB). This study was successful in developing and optimizing the mRT-PCR for these three pathogens in a single reaction test. Testing 67 field samples from Sukabumi district revealed the presence of several targeted viruses. Key Words: Screening Test, Multiplex RT-PCR, Newcastle Disease, Avian Influenza, Infectious Bronchitis

Follicle development and FSH secretion pattern of Ongole crossbred cow with natural twin birth history ., Aryogi; E, Baliarti; ., Sumadi; ., Kustono
Indonesian Journal of Animal and Veterinary Sciences Vol 18, No 3 (2013)
Publisher : Indonesian Animal Sciences Society

A research was done to obtain basic data about influence of natural twin birth history on reproduction of Ongole crossbred (PO) cow, as a basic consideration in utilizing twin genetic potency to increase beef production. The research was done for three estrus cycles respectively, and consists of two activities. The first activity was done in Beef Cattle Research Station (BCReS) at Grati sub-district, Pasuruan district â East Java, to observe number and development of follicle of ten cows with natural twin birth history (TP) and ten cows with single birth history (SP). In this activit ultra sonography (USG) equipment was used. The second activity was done in BCReS and in the Faculty of Veterinary Airlangga University at Surabaya, to observe concentration and profile of FSH using Bovine Blood Serum kit and IRMA method of five cows with TP and five PO cows with SP. Data obtained were analyzed using Chi-square test, t-test and descriptive presentation. Result shows that 23.33% of cows with TP produced two dominant (de Graf) follicles in an estrus cycle and significantly (P < 0.01) higher FSH secretion concentration (1.26 â 3.13 times) than that of cows with SP. It is concluded that PO cows with TP can produces more than one de Graf follicles in an estrus cycle and it has very high secretion concentration level of FSH. Key Words: Twin Births, PO Cow, Follicle, FSH

Effect of different diluents on the quality of sperm sexing cows with a gradient albumin (egg white) M, Ervandi; T, Susilawati; S, Wahyuningsih
Indonesian Journal of Animal and Veterinary Sciences Vol 18, No 3 (2013)
Publisher : Indonesian Animal Sciences Society

Damage of spermatozoa membrane in sexing procces with albumin gradient can decrease the quality of spermatozoa, thus to solve that need diluents for protect spermatozoa membrane to make good quality. The aim of this research is to know the best diluents between Andromed and CEP2 (cauda epididymal plasma) + 0% of yolk to keep the quality of Limousin Cowâs Spermatozoa from sexing procces with albuminâs gradient. Parameters measured for the quality of spermatozoa were : spermatozoa motility, spermatozoa viability, spermatozoa abnormality, spermatozoa concentration, and spermatozoa motility, membrane integrity, spermatozoa capacity, and acrosom reaction. Observation of membrane integrity using HOS (Hypoosmotic Swelling Test), observation of capacity and acrosom reaction using fluoresen CTC (Chortetracycline) dye. Result show that Andomed diluents and CEP2 + 10% yolk in top layer and bottom (X) and (Y) layer can keep the quality of Limousin Cowâs spermatozoa in sexing procces include spermatozoa motility (X 62,5%, Y 58%,) (X 56,5%, Y 55%), viability (X 91,64%, Y 91,51%) (X 89,87%, Y 93,51%, spermatozoaâs concentration (X 643 million /ml, Y518 million /ml) (X648 million/ml, Y517 million/ml), total spermatozoa are motil (X373,60 juta/ml, Y187,80 million/ml) (X 296,03 miliion/ml, Y 187,38 million/ml) , and have low abnormality (X 4,11%, Y 4,38%), (X 6,42%, Y 4,04%). Andromed diluents and CEP2 + 10% yolk can keep the integrity of spermatozoa membrane (X 87,60%, Y 80,26%) (X 79,61%, Y 81,03%), remains well and have the spermatozoa are not yet capacitation(X 87,60%, Y83,71%) (X 79,61%, Y 81,03%) remains high, spermatozoa capacity (X 9,76%, Y 9,92%) (X 13,45%, Y 8,95%), dan acrosom reaction (X 4,11%, Y 4,38%), (X 6,42%, Y 4,04%) remains low. Andromed diluents was not different (P > 0,05) from CEP2 diluents + 10% yolk in keeping the quality of Limousin Cow Spermatozoa from the sexing process with albuminâs gradient (egg white). Key Words: Semen, Andromed, CEP+10% Yolk, Albuminâs Gradient, Sexing

Female calves born resulted from an artificial inseminatioan (AI) using sexed sperm separated with egg albumin coloum p, Situmorang; RG, Sianturi; DA, Kusumaningrum; ., Ross; ., Maidaswar
Indonesian Journal of Animal and Veterinary Sciences Vol 18, No 3 (2013)
Publisher : Indonesian Animal Sciences Society

An efforts to alter the sex ratio of calves born could be made by separating spermatozoa bearing X and Y chromosome. The object of this study is to increase the female dairy calves born resulted from artificial insemination using the sexed sperm. There were 2 activities conducted which were 1) Spermatozoa separation based on two egg albumin column with a concentration of 10% as the upper column (UC) and 30% as the lower column (LC) and 2) AI using semen from UC. Experiment designed was completely randomized designed with three separation time (10, 20 and 30 minutes) for the treatments. Semen from UC which is expected rich in spermatozoa X, diluted in Tris Citrate extender to give a final concentration of 100 million sperm/cc and frozen to - 196Â°C for AI purposes. Results showed that separation of semen using egg albumin column, affects the size of head of spermatozoa. The means of length, width and area size of head spermatozoa were 8.7 Âµm, 4.7 Âµm, 35.7 Âµm2 and 8.4 Âµm, 4.6 Âµm, 33.7 Âµm2 for UC and LC respectively. The livability of sperm from UC and LC were not statistically significant different. The mean percentage of motile (%M), live sperm (%L) and intact apical ridge (%IAR) were 77.8, 85.5, 78.1 and 76.1, 83.5, 78.4 for UC and LC respectively. Separation time did not significantly affect the size of spermatozoa heads of UC semen. The mean length, width and area size of head of spermatozoa were 8.7 Âµm, 4.5 Âµm, 36.1 Âµm2; 8.7 Âµm, 4.8 Âµm, 36.2 Âµm2 and 8.5 Âµm, 4.7 Âµm, 34.8 Âµm2 for 10; 20 and 30 minutes respectively. From total of 160 calves born showed that the female calves born was 65%. It is concluded that AI using sexed sperm separated with egg albumin could increase the percentage of female calve born. Key Words: Egg Albumin, Column, Separation Time, Size Sperm, Female Calve

Development of spermatogenic cells and sperm quality after administration of pegagan extract (Centella asiatica) M, Solihati; B, Purwantara; I, Supriatna; A, Winarto
Indonesian Journal of Animal and Veterinary Sciences Vol 18, No 3 (2013)
Publisher : Indonesian Animal Sciences Society

The purpose of this study was to determine the development of spermatogenic cells and sperm quality after administration extracts of pegagan (Centella asiatica) in various doses and duration of administration. The research was carried out with complete randomized design (CRD), consist of 16 combinations of dose and duration of treatment. Parameters measured consist of population of spermatogenic cells (spermatogonia, primary spermatocytes, late spermatids) and sperm quality (concentration, motility, abnormality). The data were processed using analysis of variance (ANOVA), and differences between treatments followed by Duncan test. The results show that both the dose and duration have very significantly (p < 0.01) affect on decreasing of late spermatid population, sperm motility and concentration, but not for population of spermatogonia, primary spermatocytes and sperm abnormalities. The decrease of population and quality may due to antifertility effect of pegagan, eventhough still in the normal range. It is concluded that spermatogenic cells development and sperm quality reduce after administration of pegagan extract, although infertility is not yet found up to the dose of 450 mg/kg BW for 49 days duration of administration. Key Words: Spermatogenic, Sperm Quality, Pegagan Extract

Epididymal sperm quality of buffelous with Different Spotted Types Y, Yulnawati; M, Rizal; H, Maheshwari; RR, Noor; C, Sumantri; A, Boediono
Indonesian Journal of Animal and Veterinary Sciences Vol 18, No 3 (2013)
Publisher : Indonesian Animal Sciences Society

The significant decline of spotted buffalo population nowadays brought this species into an endangered situation. To perform an integrated conservation project, we need some basic information and data related to the reproductive and genetics potency of this buffalo. The purpose of this study was to observe the effect of coat color variation to the sperm quality, in order to get focus on specific candidate gene that allegedly bring the causative mutation(s) and responsible for the different pigmentation expression. In this study, we compare the quality of fresh and frozen-thawed epididymal sperm from 12 spotted bulls (that classified in 3 different spotted types, i.e Saleko, Bonga, and Lotong Boko) with five solid bulls. The results showed that there were no significant differences (P > 0.05) in all parameters of fresh and frozen-thawed epididymal sperm among those groups. The percentage of frozen-thawed progressive motility from Saleko, Bonga, Lotong Boko, and Solid was 44%, 42%, 40% and 42%, respectively. Moreover, the percentage of livability and membrane integrity of frozen-thawed sperm from each groups were 64.9%; 65.2%; 62.6%; 62.7% and 64.6%; 67.1%; 64.5%; 64.1%. In conclusion, it suggested that the coat color/phenotype difference has no effects on the quality of fresh and frozen-thawed epididymal sperm of spotted buffalo. Key Words: Epididymal Sperm, Spotted Buffalo

Effect of different levels of social isolation on the acoustical characteristics of sheep vocalization SEC, Engeldal; ., Subandriyo; E, Handiwirawan; RR, Noor
Indonesian Journal of Animal and Veterinary Sciences Vol 18, No 3 (2013)
Publisher : Indonesian Animal Sciences Society

In farm animals, vocal analysis is accepted as a non-invasive method for assessing animal welfare in comparison to most physiological measurements. The aim of this study was to investigate the influence of different degrees of social isolation on the acoustic characteristics of stress-induced bleats from three different breeds of sheep. The experiment consisted of subjecting the animals to three different levels of social isolation during which both locomotive and vocal data were recorded. The data on locomotive behaviour was subjected to descriptive analysis while specialized acoustic software was used to measure a total of thirty-six parameters of recorded bleats. Descriptive analysis showed that the animals displayed more locomotive activity during partial isolation compared to complete isolation. Number of bleats during partial isolation was also found to be higher. The application of two-way analysis of variance showed a significant effect of isolation level and breed on both temporal and structural acoustic properties. Amplitude, power and time acoustic properties were found to affect acoustic quality of vocal responses to isolation, whereas frequency related properties were also found to be different significantly (P < 0.05) between breeds. From spectrogram analysis, the patterns of energy distribution within the calls proved to offer the most evident that differ between isolation levels and breeds. It was concluded that acoustic analysis can be helpful in revealing the affective state of socially isolated rams. Key Words: Sheep, Social Isolation, Vocalization, Behaviour, Animal Welfare

Characterisation polymorphims of genotype microsatellite in association with body weight of Aceh cattle EM, Sari; N, Yunus; H, Jianlin; ., Muchti
Indonesian Journal of Animal and Veterinary Sciences Vol 18, No 3 (2013)
Publisher : Indonesian Animal Sciences Society

The objectives of this research was to evaluate polymorphism of microsatellite DNA through the investigation of loci BM1824, SPS115, and ILSTS028, and their possible association with body weight of Aceh cattle. A total of 121 of DNA samples were collected from Aceh cattle population in Indrapuri. Genetic polymorphism of Aceh cattle was calculated in frequency of alleles and genotypes. Association between genotypes and body weight was calculated by general linear model (GLM). Result showed that three loci showed high polymorphism. BM1824 locus had 11 genotypes with 3 highest frequency for BC (30.87), CC (17.28) then BB (14,82), while frequency of the other 8 genotypes namely : AB, AC, AD, AF, BD, BE, BF, and CD were less than 10 %. SPS115 locus had 9 genotypes with two highest frequency for CE (30.00) and BE (20.00), while the other 7 genotype were less than 15%. ILSTS028 locus had 22 genotypes with two highest frequencies for CH (24.69) and CC (13.59) respectively. It is conduded that Aceh cattle possessing typical genotypes of BE in BM1824 locus, AE in SPS115, and BG in ILSTS028 locus was apparently related to a higher body weight compared to other genotypes. On the contrary, those possessing AB in BM1824 and CK genotypes in ILSTS028 were seemingly associated with a lower body weight. Key Words: Aceh Cattle, Microsatellite, Body Weight

Effect of katuk leaf meal and mulberry leaf meal in the diet on size and mineral content of tibia bone of laying quail W, Hermana; T, Toharmat; ., Sumiati; W, Manalu
Indonesian Journal of Animal and Veterinary Sciences Vol 18, No 3 (2013)
Publisher : Indonesian Animal Sciences Society

The objectives of this research was to study tibia bone mineral content and blood profil of laying quail offered control diet (P0); diet with 10% katuk leaf meal /KLM (P1); diet with 10% mulberry leaf meal/MLM (P2); and diet with 5% KLM+5% MLM (P3). A completely randomized design using 4 treatments, 5 replications, and 2 quails of 17 weeks of age in each experimental unit replication was used in this experiment. The data were analyzed using analysis of variance and Duncan test. Parameters observed were tibia bone mineral content. The result showed that quail tibia bone weight and percentage of tibia bone weight were not affected by treatment, but diametre of tibia bone from quail fed mixture of KLM+MLM in the diet shorter (P < 0.05) than that of the other treatment. It wasÂ concluded that KLM and MLM can be used in the diet of laying quail up to 10% without affected the tibia bone mineral content. Key Words: Katuk Leaf Meal, Laying Quail, Mulberry Leaf Meal, Tibia Bone.

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Ahmadi Riyanto

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Home Page

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Editorial Team

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Contact Name Ahmadi Riyanto

Contact Email medpub@litbang.deptan.go.id

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Location Kota bogor, Jawa barat INDONESIA

Abstract

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Abstract

Contact Name
Ahmadi Riyanto

Contact Email
medpub@litbang.deptan.go.id

Phone
-

Journal Mail Official
ahmadi_puslitbangnak@yahoo.com

Editorial Address
-

Location
Kota bogor,

Jawa barat

INDONESIA