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Jurnal Ilmu Ternak Veteriner

JITV
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Published by Indonesian Center for Animal Research and Development

ISSN : 08537380 EISSN : 2252696X DOI : -

Core Subject : Health,

Veterinary

Aims JITV (Jurnal Ilmu ternak dan Veteriner) or Indonesian Journal of Animal and Veterinary Sciences (IJAVS) aims to publish original research results and reviews on farm tropical animals such as cattle, buffaloes, sheep, goats, pigs, horses, poultry, as well as non domesticated Indonesian endemic animals, such as deers, anoa, babirusa, etc. Scope Indonesian Journal of Animal and Veterinary Sciences . The journal will consider primary research papers from any source if they make an original contribution to the experimental or theoretical understanding and application of theories and methodologies of some aspects of animal science and veterinary

Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Hewan dan Zoologi

Articles 1,756 Documents

163 164 165 166 167

Epidemiological study of brucellosis and its effect on reproduction failures in dairy cattle in DKI Jakarta Agus Sudibyo
Jurnal Ilmu Ternak dan Veteriner Vol 1, No 1 (1995)
Publisher : Indonesian Center for Animal Research and Development (ICARD)

The purposes of this research were to observe prevalence, distribution and reproduction failure evidence of dairy cattle brucellosis in DKI Jakarta . To determine the group of cattle infected with Bntcella abortus, senim and milk samples were carried out . At the beginning, bulk of milk samples were collected from containers . Futhermore, the blood samples were simple randomly collected on cattle that were suspected positive brucellosis in the milk ring test . Those serum samples were tested by using rose bengal plate and complement fixation test . Questioner data in relation with on any reproductive failures, such as abortion, premature dead and born weak, were gathered from farmer within 8 consecutive month period . The result indicate that screening of brucellosis using milk ring test based on bulk milk sample was effective tools to determine the infected group of cattle . The average prevalence of brucellosis in DKI Jakarta area was 4 .5 %. Distribution of brucellosis prevalence were 8 .5% at Setiabudi, 2 .3% at Mampang Prapatan, and 2 .9% at Pasar Minggu . Brucellosis was found at least 201 cattle from 38 dairy cattle farm in DKI Jakarta . Brucellosis was caused abortion about 62 .5% dairy cattle and this value was significantly different than that of uninfected cattle (P<0.05) . Brucellosis also cause stillbirth around 9 .8% and born weakness 15 .2% . The widely distributed and high prevalence brucellosis of dairy cattle in DKI Jakarta was influenced by uncontrol of cattle movement and high abortion cases rate .

Effects of Actinobacillus pleuropneumoniae cytotoxins on generation of oxygen radicals by porcine neutrophils Simson Tarigan
Jurnal Ilmu Ternak dan Veteriner Vol 4, No 1 (1999): MARCH 1999
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Cytotoxins produced by Actinobacillus pleuropneumoniae (App) suggested to be the most important pathogenic and virulent factors for this organism. However, the mechanisms on how the cytotoxins contribute to the disease process remain unclear. The purpose of this study is to investigate the effect of the cytotoxins on the oxidative-burst metabolism of porcine neutrophils. In this study, neutrophils were firstly loaded with an oxidative probe dichlorofluorescin diacetate (DCFHDA) then expose to cytotoxins. Cells producing oxygen radicals emitted fluorescence and its intensity was measured with a FACScan flow cytometer. All cytotoxins derived from either App serotypes producing ApxI and ApxII, App serotypes producing ApxII only, or App serotypes producing ApxII and ApxIII were capable of stimulating neutrophils for oxygen-radical generation. However, compared with phorbol myristate acetate (PMA), App cytotoxins were much weaker as stimulants for oxygen radicals. In addition, Apx preparation stimulated an oxidative-burst metabolism of neutrophils at a low, narrow range of Apx doses. At higher doses, the toxins inhibit the oxidative burst metabolism. The effects of cytotoxins produced by App during infection on recruited neutrophils into the lungs are assumed to be comparable to those observed in this in vitro study. Neutrophils, and other host cells, adjacent to the bacteria become lysis due to high toxin concentration, whereas those at some distance to the bacteria produce oxygen radicals which in turn cause tissue damage or necrosis. Key words: Actinobacillus pleuropneumoniae, cytotoxin, Apx, neutrophils, pig, oxygen radical, flow cytometry

Effect of pregnant mare serum gonadotrophin injection on litter size in young Etawah-cross does N.M Artiningsihi; B Purwantara; R.K Achjadi; I-K Sutama
Jurnal Ilmu Ternak dan Veteriner Vol 2, No 1 (1996)
Publisher : Indonesian Center for Animal Research and Development (ICARD)

The incidence of twins and/or multiple births in 20 heads of young Etawah-cross does was studied following oestrous synchronization using intravaginal sponges containing 60 mg medroxyprogesterorle acetate (Repromap) for 15 days . Twenty four hours priorto sponges withdrawal, the does were injected with pregnant mare serum gonadotrophin (PMSG) at dose rates of 0 (Group A), 10 (Group B), 15 (Group C) and 20 iu/kg (Group D) body weight. Amature buck fitted with an apron was used to detect the onset of oestrus at every four hours. The oestrous doe was naturally mated twice, 12 hours after onset of oestrus and 10 hours later. About 3-5 days after oestrus, all does were subjected to mid-ventral laparoscopy to detect ovulation rate . Two months after mating all does were subjected to pregnancy test using diagnostic ultrasonography. Results showed that all does exhibited clear sign of oestrus. The onset of oestrus occurred 15-43 hours after sponges withdrawal or 39-59 hours after PMSG injection. Does injected with PMSG (Groups B, C and D) showed oestrus 16-21 hours earlier (P<0.05), and it was 1 .6-4.8 hours longer (P>0 .05) than that of control (Group A) . However, there was no significant differences among the PMSG-treated groups . Ovulation rates increased from 1.0 in Group Ato 1.8 in Group B and 2.6 in bah Groups Cand D. Average litter size in Groups A, B, C and D were 1.0, 1.8, 2.4 and 1.0, respectively. It was concluded that injection of 15 iu PMSG/kg body weight gave the best result for increasing litter size in young Etawah-cross does . Key words: Etawah-cross, PMSG, synchronization, reproduction

The phenotypic detection of surface antigen of B lymphocytes, MHC I and MHC II by immunohistochemical techniques in the lymph nodes of Bali cattle infected with malignant catarrhal fever Rini Damayanti
Jurnal Ilmu Ternak dan Veteriner Vol 4, No 4 (1999): DECEMBER 1999
Publisher : Indonesian Center for Animal Research and Development (ICARD)

A study on the phenotypic detection of surface antigen of B lymphocytes, MHC I and MHC II in lymph nodes of Bali cattle affected by malignant catarrhal fever (MCF) was conducted by immunohistochemical techniques using avidin-biotin complex peroxidase methods. A number of monoclonal antibodies against surface antigen of B lymphocytes, MHC I and MHC II were used. The results showed that the surface antigens were all detected either in the MCF or in the non-MCF Bali cattle lymph nodes. MHC I were shown predominantly occupied in the lymph nodes of infected cattle. The surface antigen of B cells and MHC II were less found in the infected ones. This indicates that in the immunopathological processes in MCF infected cattle, B cells were not actively involved in producing antibodies, whereas MHC I may contribute the high degree of susceptibility of Bali cattle to MCF. Key words : MCF, B cells, MHC I, MHC II, Bali cattle, immunohistochemical technique

Reciprocal crossing between Tegal and Mojosari ducks : I . Early gowth and early egg production L. Hardi Prasetyo; Triana Susanti
Jurnal Ilmu Ternak dan Veteriner Vol 2, No 3 (1997)
Publisher : Indonesian Center for Animal Research and Development (ICARD)

In !ndonesia, duck farming plays an important role in meeting the demand for eggs and as an alternative source of income for small farmers . However, the production efficiency of duck farming is still very low because of small ownership and low quality of breeding stock . One way of improving the quality of breeding stock is by using crossbreds from various breeds of local ducks to exploit heterosis which may arise from the crossings . In order to test the performance of Tegal and Mojosari crossbreds, 250 ducks each of Mojosari and Tegal breeds were used and crossed to produce TT (Tegal x Tegal), TM (Tegal x Mojosari), MT (Mojosari x Tegal), and MM (Mojosari x Mojosari) . In this report, observations were taken only during the early gowth and the first egg laying. Results show that crossbreds between Mojosari did and Tegal not show any superiority to the parental breeds on young drakes at early gowth, while on young female ducks, the crossbred TM even showed a smaller body weight gain up to 8 weeks, although still similar to MM. The crossbred MT approached the performance of TT which is higher than TM or MM. This indicates a strong maternal effect, which was also confirmed by the findings on the age at first laying . In this early stage of gowth, there is no significant heterosis among the crossbreds. This confirms that these duck breeds can not be expected as meat producers . Observation of heterosis on components of egg production will describe more of the production potentials of these breeds. Keywords : Duck, crossbreeding

Fractionation, identification and vaccination efficacy of native antigens from the screwworm fly, Chrysomya bezziana George Riding; Sri Muharsini; Roger Pearson; Sukarsih .; Edy Satria; Gene Wijffels; Petter Willadseni
Jurnal Ilmu Ternak dan Veteriner Vol 5, No 3 (2000): SEPTEMBER 2000
Publisher : Indonesian Center for Animal Research and Development (ICARD)

sources of potential protective antigens from the sheep blowfly Lucilia cuprina. Their importance in the screwworm fly Chrysomya bezziana has now been investigated. Purified serine proteases from Chrysomya bezziana were tested for their potential as vaccine antigens in sheep, efficacy being assessed by in vitro and in vivo assays with larval Chrysomya bezziana. No effect of vaccination was observed by the in vitro assay. However, in the in vivo challenge, larval weights were diminished in the vaccinated sheep, although larval recoveries increased marginally. Vaccination with Chrysomya bezziana peritrophic membrane does induce an effective immune response against the parasite resulting in a significant reduction in larval growth and considerable larval mortality in the in vitro assay. Sequential fractionation of the peritrophic membrane with various surfactants and chaotrophic agents of increasing solubilisation capacity resulted in the separation of discrete groups of proteins. The groups of fractionated proteins were tested in a vaccination trial in sheep with vaccine efficacy assessed by in vitro assays. The urea extract, guanidine-HCl extract and SDS soluble fraction each induced significant levels of protection against Chrysomya bezziana larvae but the effects were poorer than those obtained from vaccination with whole, native peritrophic membrane. Several major proteins selected from the three most protective fractions were purified by SDS polyacrylamide gel electrophoresis. Since insufficient quantities of these proteins were available for vaccination trials, they were either sequenced directly from the N-terminus or subjected to endoproteinase Lys-C digestion, followed by peptide purification and amino acid sequencing. This gave the information necessary for the expression of several of these roteins as recombinants in a form suitable for vaccination studies. Key words: Chrysomya bezziana, peritrophic membrane, vaccination, amino acid sequence, serine protease

Isolation and identification of indigenous lactic acid bacteria from North Sumatra river buffalo milk Heni Rizqiati; Cece Sumantri; Ronny Rachman Noor; E. Damayanthi; E. I. Rianti
Jurnal Ilmu Ternak dan Veteriner Vol 20, No 2 (2015): JUNE 2015
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Buffalo milk is a source of various lactic acid bacteria (LAB) which is potential as culture starter as well as the probiotic. This study was conducted to isolate and identify LAB from indigenous North Sumatra river buffalo milk. Lactic acid bacteria was isolated and grown in medium De Man Rogosa Sharpe Agar (MRSA). The isolation was conducted to obtain pure isolate. The identification of LAB was studied in terms of morphology, physiology, biochemistry and survival on low pH. Morphology tests were conducted by Gram staining and cell forming; physiology tests were conducted for growing viability at pH 4.5 and temperature at 45oC; whereas biochemistry tests were conducted for CO2, dextran and NH3 productions. Determination of LAB species was conducted using Analytical Profile Index (API) test CHL 50. Results of identification showed that 41 isolates were identified as LAB with Gram-positive, catalase-negative, rod and round shaped characteristics. Resistance test done to low pH (pH 2) for the lactic acid bacteria showed decrease of bacteria viability up to1.24±0.68 log cfu/ml. The resistant isolates at low pH were L12, L16, L17, L19, L20, M10, P8, S3, S19 and S20. Identification with API test CHL 50 for 10 isolates showed that four isolates were identified as Lactobacillus plantarum, L. brevis, L. pentosus and Lactococuslactis.

The competitive interaction of snails Thiara scabra and Physa doopi on the snail Lymnaea rubiginosa under laboratory conditions Sarwitri Endah Estuningsih
Jurnal Ilmu Ternak dan Veteriner Vol 3, No 1 (1998)
Publisher : Indonesian Center for Animal Research and Development (ICARD)

The competitive interaction of the snails Thiara scabra and Physa doopi against the snail Lymnaea rubiginosa, the intermediate host ofFasciola gigantica has been studied. Aquaria, 60 x 40 x 20 cm and 30 x 20 x 10 cm in size were used, and the two snail species were introduced in different proportion . The results over a one year period showed that the competition between snail L. rubiginosa and T. scabra or P. doopi has started after 8 months as demonstrated by the reduction of the population ofL. rubiginosa and increasing the population of T. scabra or P. doopi. The competitive interaction does not seem to be due to competition for food but to a chemical factors, possibly water-soluble pheromones. The other explanation is discussed . Keywords : Snail, intermediate host, Limnaea rubiginosa, Thiara scabra, Physa doopi, Fasciola gigantica, competitive Interaction

Utilization of palm oilsludge in poultry diet: 3. Inclusion of fresh or dried fermented palm oil sludge in broiler’s diet A.P Sinurat; Tatty Purwadaria; T Pasaribu; J Darma; I.A.K Bintang; M.H Togatorop
Jurnal Ilmu Ternak dan Veteriner Vol 6, No 2 (2001): JUNE 2001
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Drying proces, especially with heating often affects the nutritive values of feed ingredients. Therefore, this experiment was designed to study the responses of broiler chickens when fed with ration containing fresh or dried fermented palm oil sludge(FPOS). Experimental diets with different levels of fresh or dried FPOS (5, 10, and 15% equally to dried FPOS) were formulated with similar nutrient contents. A control diet with no FPOS was also included. Each diet was fed to 30 broiler chickens (5 replicates of 6 birds) for 5 weeks. Performances (body weight, feed consumption, feed conversion, and mortalities) of chickens were recorded. Carcass percentage and abdominal fat content was also measured at the end of feeding trial. Data obtained were subjected to analysis of variance in a completely randomized design and different between means were tested by orthogonal contrast procedures. Results of the experiment showed that body weight gain (BWG) of control birds was not significantlydifferent with BWG of birds fed with FPOS. Birds fed with dried FLS gain more weight than those fed with fresh FPOS (1048 vs 981 g/bird). Increasing of dietary FPOS levels decreased BWG, but 10% inclusion was still tolerable. Feed consumption of control diet was significantly (P<0.05) less than feed consumption of diet with FPOS. Increasing of dried FPOS to 15% did not affect feed consumption, but increasing of fresh FPOS significantly increased feed consumption. Feed conversion (FCR) of control diet was significantly better than the FPOS diet (2.07 vs 2.13). Increasing levels of dried FPOS from 5 to 10% did not affect the FCR, but further increasing to 15% significantly worsen the FCR. Increasing of fresh FPOS from 5 to 10 or 15 significantly worsen the FCR. Dry matter intake, mortalities, carcass percentage, liver weight, and abdominal fat levels of broilers were not significantly affected by dietary treatments (P>0.05). Conversion of feed dry matter to body weight gain of control diet was not significantly (P>0.05) different with those diets with FPOS. However, dry matter conversion of dried FPOS was significantly better than the fresh FPOS. Increasing levels of FLS from 5 to 15 significantly worsen the feed dry matter conversion but not with inclusion of 10% FPOS. Therefore, it is concluded that there is no superior advantage of feeding fresh FPOS as compared with dried FPOS. Inclusion of 10% dried or fresh FPOS in the diets did not affect growth performances of broiler chickens. Key words: Palm oil sludge, dried, fermented, broilers

The mucosal and systemic immune responses in chickens orally immunised with Campylobacter jejuni antigen entrapped in poly-lactide-co-glycolide microparticles Susan Maphilindawati Noor
Jurnal Ilmu Ternak dan Veteriner Vol 3, No 4 (1998)
Publisher : Indonesian Center for Animal Research and Development (ICARD)

An oral immunisation in chickens with antigen entrapping in biodegradable microparticles was evaluated in order to achieve optimal antibody responses following oral vaccination. This study was adapted to Campylobacter jejuni antigen in chickens to observe its stimulation both mucosal and systemic immune responses. A group of 5 embryonated chicken eggs was immunised with heat-killed C. jejuni entrapped in poly-lactide-co-glycolide (PLG) microparticles at day 17 of incubation deposited into the amniotic fluid. Seven days after hatching the chickens were oral boostered, these was design to as Group A. In the Group B, each embryonated egg was immunised with soluble C. jejuni as in the same as Group A. Immune responses of post vaccination were observed at day-14, the humoral immunity was evaluated with an ELISA and whereas mucosal antibody response was detected by fluorescent histology. The serum IgG and IgA antibody responses, and also the bile and intestinal scrapping IgA antibody responses to campylobacter in Group A were significantly higher than those of the soluble antigen of Group B (P<0.05). Total number of immunoglobulin-containing cells for IgG, IgM, and IgA isotypes in the ileum in Group A chickens were also significantly higher than those of Group B (P<0.05) but was not in the duodenum and spleen. Key words : Campylobacter jejuni, poly-lactide-co-glycolide microparticles, oral immunisation, chickens

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Home Page

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Contact Name Ahmadi Riyanto

Contact Email medpub@litbang.deptan.go.id

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Location Kota bogor, Jawa barat INDONESIA

Abstract

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Contact Name
Ahmadi Riyanto

Contact Email
medpub@litbang.deptan.go.id

Phone
-

Journal Mail Official
ahmadi_puslitbangnak@yahoo.com

Editorial Address
-

Location
Kota bogor,

Jawa barat

INDONESIA