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Jurnal Ilmu Ternak Veteriner

JITV
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Published by Indonesian Center for Animal Research and Development

ISSN : 08537380 EISSN : 2252696X DOI : -

Core Subject : Health,

Veterinary

Aims JITV (Jurnal Ilmu ternak dan Veteriner) or Indonesian Journal of Animal and Veterinary Sciences (IJAVS) aims to publish original research results and reviews on farm tropical animals such as cattle, buffaloes, sheep, goats, pigs, horses, poultry, as well as non domesticated Indonesian endemic animals, such as deers, anoa, babirusa, etc. Scope Indonesian Journal of Animal and Veterinary Sciences . The journal will consider primary research papers from any source if they make an original contribution to the experimental or theoretical understanding and application of theories and methodologies of some aspects of animal science and veterinary

Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Hewan dan Zoologi

Articles 1,756 Documents

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Antigen-capture enzyme-linked immunosorbent assay using monoclonal antibody for detection of bluetongue virus antigen An Sendow, Indrawati
Indonesian Journal of Animal and Veterinary Sciences Vol 2, No 4 (1998)
Publisher : Indonesian Animal Sciences Society

antigen-capture enzyme-linked immunosorbent assay (ELISA) using a specific anti-bluetongue virus group was applied to detect bluetongue viral antigen . The test was specific for bluetongue viruses and did not detect the closely related epizootic haemorrhagic disease of deer viruses (EHDV) and other orbiviruses . It was easy to perform and could be established in laboratories which have simple facilities . The antigen-capture ELISA technique is an alternative method to agar gel immunodiffusion and immuno-dot blotting tests to detect bluetongue antigen in infected tissues, Vero cells, Aedes albopictus cells and BHK-21 cell cultures . Â Keywords : ELISA, monoclonal antibody, antigen detection, BT

Haemophilus paragallinarum in chickens in Indonesia : II . Physiological and biochemical properties of Haemophilus spp . from diseased chickens Poernomo, Sri; ., Sutama; Nazrudin, Yapto
Indonesian Journal of Animal and Veterinary Sciences Vol 2, No 4 (1998)
Publisher : Indonesian Animal Sciences Society

A total of 46 isolates of Haemophilus spp . consisted of 42 local isolates from diseased chickens and four reference strains of Haemophilus paragallinarum were examined for their physiological and biochemical properties . These isolates consisted of 2 groups. One group consisted of 21 local isolates and reference strains of H. paragallinarum were catalase negative and identified as Haemophilus paragallinarum and they did not ferment galactose and trehalose . The other group was catalase positive and suspected as Haemophilus avium . This catalase positive group of these 21 avian Haemophili should be examined with other carbohydrates in order to be identified accurately . Â Keywords : Haemophilus spp ., physiological, biochemical, chickens

Haemophilus paragallinarum in chickens in Indonesia: III. Antimicrobial drug sensitivity test ofHaemophilus paragallinarum from chickens suffering of coryza Poernomo, Sri; Sutarma, Sutarma; Dewi Silawatri, Sang Ayu Ketut
Indonesian Journal of Animal and Veterinary Sciences Vol 2, No 4 (1998)
Publisher : Indonesian Animal Sciences Society

An agar disc diffusion method was used to examine the sensitivity of 27 Haemophilus paragallinarum (Hpg) isolates consisted of 23 local isolates, 4 standard isolates (serotype A) and Escherichia coli ATCC 24922 as a control to eight antimicrobial drugs (ampicillin, erythromycin, oxytetracycline, doxycycline, neomycin, streptomycin, colistine and sulphanlethoxazole-trimethoprim) . Twenty one out of 23 local isolates of Hpg were sensitive to doxycycline, 19 isolates to ampsllin, 18 isolates to oxytetracycline, 17 isolates to sulphametoxazole-trimethoprim, 16 isolates to erythromycin, and 13 isolates to neomycin, while 13 isolates were resistance to colistine and 11 isolates were also resistance to streptomycin . Â Keywords : Haemophilus paragallinarum, chicken, coryza, sensitivity test, antimicrobial drug

An estimate of seasonality and intensity of infection with gastrointestinal nematodes in sheep and goats . in West Java ., Beriajaya; Copeman, D.B
Indonesian Journal of Animal and Veterinary Sciences Vol 2, No 4 (1998)
Publisher : Indonesian Animal Sciences Society

Tracer Inoodle tbin-tail sheep and Kacang goats were used to measure the seasonal changes in gastrointestinal nematodes parasitism i ~und[, .xillapek .Fodd~ ofis in West Java . Each 3 months for 12 months worm-free male sheep (5) and goats (5) about 5 months of age were ~ $Jri~tgldI o% ef- farmer, and managed as part of their flock for 2 months . Animals were then returned to the laboratory and maintained on "`~^taaan-ftwAiet in elevated slatted pens for 3 weeks prior to slaughter. In all Â trials sheep had higher faecal egg counts than goats . Egg counts were significantly lower during the late dry-early wet season due mainly to lower burdens of Oesophagostomum spp. than at other times of the year. The predominant genera recovered from faecal larval cultures were Haemonchus and Trichostrongylus . At post mortem more than 94 percent of animals were infected with Trichostrongylus colubriformis, T. axei, Haemonchus contortus, Oesophagostomum columbianwn and Strongyloides papillosus . Other species found, in descending order of occurrence, were Cooperia curticei, Trichuris ovis, Bunostornum trigonocephalum, Oesophagostomumn asperum, Capillaria bovis and Gaigena pachycelis. It was concluded that intensity of exposure of both sheep and goats to H contortus, T. axei and C. curticei was similar throughout the year, but that availability of infectioe larvae of T. colubriformis was higher during the dry than the wet season and vise versa for O. columbianum . Sheep had higher burdens of T. Colubrzformis than goats but similar numbers of other species. Â Keywords: Thin-tail sheep, kacang goats, tracer, gastrointestinal nematode

Comparative advantage and sensitivity analysis of dairy farms by development patterns in West Java Rachman, Benny
Indonesian Journal of Animal and Veterinary Sciences Vol 3, No 1 (1998)
Publisher : Indonesian Animal Sciences Society

The study is aimed at analyzing feasibility of the dairy farm on several development scheme. Domestic resource cost (DRC) analysis is used to understand problems in dairy farms. The study was carried out during August to December 1994 in Bandung and Bogor regencies of West Java . The result of this study indicates that the milk production produced by credit pattern, recommendation pattern and farm group pattern namely, 3,800 liter/ut/year, 4,422 liter/ut/year and 4,270 liter/ut/year, respectively, and also the international market price is Rp. 375/liter tend to be efficient. This phenomena is characterized by coefficient of DRC <1 .0 . Nevertheless, in term of dairy farm development should be based on several aspects such as, economics of scale and scheme of dairy farm. Moreover, farm group pattern (PPK) is more relatively efficient than the others. Â Keyword : Domestic resource cost (DRC), credit pattern, recommendation pattern, group pattern

Growth response of Kampung and Pelung cross chickens to diet differed in protein content Iskandar, Sofjan; Z, Desmayati; Sastrodihardi, S; Sartika, T; Setiadi, P; Susanti, T
Indonesian Journal of Animal and Veterinary Sciences Vol 3, No 1 (1998)
Publisher : Indonesian Animal Sciences Society

Two lines of native chicken (Kampung and Pelung cross) were subjected to diets differed in protein content (21, 19, 17and 15%), but isocaloric (2,900 kcal ME/kg). Ten (5 males, 5 females) day old chicks were allocated as replicate and thetreatments were 2 lines x 4 levels dietary protein. The trial was conducted for 12 weeks period . Bodyweight gain of Kampungchicken (704 g/bird) was lower than ofPelung cross chicken (844 g/bird). Based on bodyweight gain and feed conversion ratio,the optimum level of dietary crude protein was 19% for both lines, whilst consumption was not affected either by lines nor bydietary protein. Pelung cross chicken converted feed to body weight more efficiently than Kampung chicken did. However feedconversion ratio increased with decreasing in dietary protein. Mortality was not affected either by lines nor by dietary protein.Twelve weeks carcass percentage of Pelung cross (64,9%) was higher than of Kampung chickens (62,9%), however carcass percentage was not affected by the dietary protein. Abdominal fat pad was not affected by lines of chickens nor dietary protein. Â Key words : Native chicken, protein, growth, carcass

Nutritive value of fermented coconut meal in ration of laying ducks with various phosphorous levels Sinurat, A.P; Purwadaria, T; Habiebie, A; Pasaribu, T; Hamid, H; Rosida, J; Haryati, T; Sutikno, I
Indonesian Journal of Animal and Veterinary Sciences Vol 3, No 1 (1998)
Publisher : Indonesian Animal Sciences Society

A series of experiment was conducted to improve the utilization of coconut meal in poultry ration . A bioprocess (fermentation) approach was adopted. Coconut meal was fermented followed by an enzymatic process at room temperature or 50 OC.The nutritive value of the fermented products was determined chemically. Product with the highest nutritive value was then used for a feeding trial to study the interaction effect between dietary levels of fermented coconut meal and phosphorous level on the performances of laying ducks. Nine experimental diets were formulated, i.e ., the factorial of 3 levels of fermented coconut meal (0, 30 and 40%) and 3 levels of total phosphorous (0.6, 0.8 and 1 .0%) . The diet was fed to pullet ducks and the performances of the ducks were observed for 16 weeks of production period. The results showed that the nutritive value of coconut meal increased by fermentation and the highest yield was achieved when the enzymatic process was performed at 500C. There was no significant interaction effect between dietary fermented coconut meal levels and the phosphorous levels on all parameters observed, except on egg weight. Inclusion of fermented coconut meal in the ration up to 30% did not produce negative effects on the productivity oflaying ducks, however, higher levels (40%) ofinclusion reduced the egg size Â Keywords: Coconut meal, fermentation, ducks, egg production

Effects of the addition of hormone in maturation medium for in vitro production ofembryo (IVP). Hidayati, Nurhasanah; Sugiarti, Tatit; Situmorang, Polmer; Triwulanningsih, Endang; Lubis, Adriana
Indonesian Journal of Animal and Veterinary Sciences Vol 3, No 1 (1998)
Publisher : Indonesian Animal Sciences Society

A study on the effects of hormone FSH, hCG and estrogen in maturation medium on embryo production (IVP) was conducted. Ovaries of dairy cows were obtained from slaughtered house and oocytes were collected by aspiration and slicing. Oocytes were matured in TCM-199 media containing one of each hormonal treatments : 10 ug/ml FSH, 2 IU hCG, 1 ug/ml estrogen, 10 ug/ml FSH + 2 IU hCG, 10 ug/ml FSH + 1 ug/ml estrogen, and 10 ug/ml FSH + 2 IU hCG + 1 ug/ml estrogen for 24 hours. Fertilization was conducted in thyroid albumin lactate pyruvate (TALP) media containing 10 ug/ml heparin for 18-24 hours and co-cultured in synthetic oviduct fluid (Sof-media) using C02 incubator at 38oC. Every 48 hours the embryos were moved into fresh Sofmedia and embryo evaluation was done on day 7using a microscope . From a total of 293 oocytes studied resulted 60.4% of zygotes which develop to 9.4% young embryos ( cell numbers <16), 45.3% morulae and 5,7% blastocysts. Hormones used singly did not significantly affect the production of embryo . The highest mean percentages of fertilization wasobtained in FSH (73.3%) and the lowest in estrogen (59.6%), but the mean percentage of blastocyst was higher in estrogen . The mean percentages of young embryos, morulae and blastocysts were 4.8, 66.1, 2.4 ; 18 .8, 41 .6, 6.6 and 3 .3, 46.6, 10 .0 for FSH, hCG and estrogen, respectively . Using a combination of FSH with hCG and estrogen did not significantly increase the production of embryo . The mean percentages of fertilization, young embryo, morulae and blastocysts were 55.2, 9.3, 43.4 and 2.6 ; 51 .7, 4.8, 35.8 and 11 .1 and 56 .0, 12 .3, 42.3 and 1 .4 for FSH+hCG, FSH+estrogen and FSH+hCG+estrogen respectively . Â Keywords: Embryo, fertilization, hormone, in vitro

The application of enterotoxigenic Escherichia coli (ETEC) K99, F41 polyvalent vaccine in pregnant dairy cattle to control neonatal colibacillosis and mortality of calves ., Supar; ., Kusmiyati; Poerwadikarta, B
Indonesian Journal of Animal and Veterinary Sciences Vol 3, No 1 (1998)
Publisher : Indonesian Animal Sciences Society

Enterotoxigenic Escherichia coli (ETEC) strains possessing either K99, F41 or K99F41 are responsible for causing neonatal diarrhoea and mortality of calves and difficult to control using antimicrobial drugs. A whole cell ETEC vaccine containing fimbrial antigens of polyvalent strains based on field serotypes was produced . The efficacy of ETEC vaccine used to control neonatal colibacillosis of dairy calves was studied in experimental animals and field trials. Five pregnant dairy cow were used for experimental study. Three animals were injected subcutaneously with 5 ml vaccine at 6 weeks and again 2 weeks before expected date of calving, others were left unvaccinated as control. Two calves born from vaccinated cows were given colostrum and milk from their own mothers. A calf born from vaccinated cow was not given colostrum, but milk from other vaccinated cow at day 8 . Three day old calves receiving colostrum of vaccinated cows were challenged with 2 ml either ETEC K99 or F41 suspension containing 108 colony forming units per ml did not show clinical signs of diarrhoea and their body weight increased progressively. Whereas, a calf born from unvaccinated group was challenged with ETEC K99 developed clinical sign of diarrhoea at 15 hours later and died at 8 days post-inoculation . A calf born from unvaccinated cow was challenged with ETEC F41 developed watery diarrhoea, it did not die, but its body weight relatively did not increase. The use of two doses ofpolyvalent ETEC vaccine at late gestation gave protection to the suckling offspring against challenged . Under farm conditions, dams vaccination with 2 doses of polyvalent ETEC vaccine 6 week and 2 weeks before expected date of calving reduced the calf mortality from average of 13% per months to 0.7%. It was concluded that dams vaccination with polyvalent ETEC containing K99 and F41 fimbrial antigens gave protection to their suckling offsprings or through consuming their colostrum or milk against homologous ETEC infection. Â Keywords: Calf, colibacillosis, ETEC, dams vaccination

Protein profiles of field isolates ofBacillus anthracis from different endemic areas of Indonesia Poerwadikarta, M Bhakti
Indonesian Journal of Animal and Veterinary Sciences Vol 3, No 1 (1998)
Publisher : Indonesian Animal Sciences Society

Sonicated cell-free extract proteins of 14 field isolates ofBacillus anthracis from six different endemic areas of Indonesia were analyzed by the use of sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) methods . The protein profiles of each field isolate tested demonstrated slightly different at the protein bands with molecular weights of 18, 37, 52, 65 and 70 kDa, and varied between the field isolates and vaccine strains. The variation could provide clues to the source of anthrax transmission whether it was originated from similar strain or not. Â Key words : Protein profiles, Bacillus anthracis, SDS-PAGE

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Ahmadi Riyanto

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Home Page

OAI Link

Editorial Team

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Contact Name Ahmadi Riyanto

Contact Email medpub@litbang.deptan.go.id

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Journal Mail Official ahmadi_puslitbangnak@yahoo.com

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Location Kota bogor, Jawa barat INDONESIA

Abstract

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Abstract

Contact Name
Ahmadi Riyanto

Contact Email
medpub@litbang.deptan.go.id

Phone
-

Journal Mail Official
ahmadi_puslitbangnak@yahoo.com

Editorial Address
-

Location
Kota bogor,

Jawa barat

INDONESIA