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Journal of Biomedicine and Translational Research
Published by Universitas Diponegoro
ISSN : -     EISSN : 25032178     DOI : -
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology
Journal of Biomedicine and Translational Research (JBTR) is an open access, international peer-reviewed journal that considers articles on: clinical medicine, molecular medicine, tropical medicine, infectious diseases, cardiovascular medicine, molecular biology, genetics, immunology, microbiology, biochemistry, and pharmacotherapy with particular interest on the link between clinical and basic research called translational research.
Arjuna Subject : -
Articles 173 Documents
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Elevated levels of serum glucose, triglyceride, and liver enzymes in a rat model of 7,12 dimethylbenz[a]anthracene (DMBA)-induced carcinogenesis Ika Yustisia; Padlianah Padlianah; Mutmainah Arif; M. Husni Cangara
Journal of Biomedicine and Translational Research Vol 8, No 2 (2022): August 2022
Publisher : Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v8i2.14268

Abstract

ABSTRACT Background: Uncontrolled cell proliferation in cancers has a high requirement of energy and biosynthetic substrates. Glucose and triglycerides are the main source of energy as well as the primary building blocks for forming cellular components of mammalian cells.Objective: This study aims to evaluate the shifting of serum glucose, triglyceride, and nitrogen wastes in the form of urea and creatinine levels; and liver enzymes levels, alanine transaminase (ALT) and aspartate aminotransferase (AST), in rat model carcinogenesis with a single dose of 7,12-dimethylbenzanthracene (DMBA) for 16 weeks observation..Methods: The experimental animals of Rattus norvegicus strain Sprague Dawley were divided into two groups, namely the control group and the DMBA-induced group. A blood chemistry examination was carried out at weeks 4, 8, 12, and 16 post-induction using a spectrophotometer. In addition, observation of breast tumor formation and histological examination of the tumor and organs, including liver, lung, intestine, and kidney, were performed to confirm cancer formation.Results: Five of the six experimental rats (83.3%) induced by DMBA experienced breast and lung cancer formation accompanied by continuous weight loss starting at week 10 after induction. Serum glucose levels increased significantly at weeks 12 and 16 after induction, while serum triglyceride, ALT, and AST levels increased significantly from week 4 after induction until the end of the experiment. Serum urea levels did not show a significant difference from the control group. Nonetheless, creatinine decreased at the last examination.Conclusion: Elevated serum glucose, triglycerides, ALT, and AST levels escorted the chemical carcinogen-induced cancer development. Studies at the clinical level are needed to prove whether abnormally elevated of these blood chemistry levels can be used to detect the presence of cancer early.Keywords:DMBA, breast cancer, lung cancer, cancer metabolism.
Comparison of Testosterone Undecanoate (TU) and Testosterone Enanthate (TE) with Combination of Depot Medroxyprogesterone Acetate (DMPA) on Spermatogenic Cells in Men Retnaningtyas Siska Dianty; Yurnadi Hanafi Midoen
Journal of Biomedicine and Translational Research Vol 8, No 2 (2022): August 2022
Publisher : Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v8i2.15318

Abstract

Introduction: Male hormonal contraception known as safe, effective, and reversiblecontraception. World Health Organization (WHO) multicentre study has been conducted aclinical study administrating of testosterone regimens and progestin to male with a remarkableresult. Azoospermia or severe oligozoospermia occurred in men with injection of TestosteroneEnanthate (TE) and Depot Medroxyprogesterone Acetate (DMPA) as well as TestosteroneUndecanoate (TU) and DMPA for a longer period. Decreasing gonadotropin as well astestosterone could lead to the amount of spermatogenic cells population declining by suppressingthe development of many kinds of cells. This narrative review was to compare howspermatogenic suppression in TE and TU with Combination of DMPA. Method: We arerandomly select article or journal from several databases and individual journal in certainkeywords. The keyword used were : (male hormonal contraception) OR (regimen testosterone))AND (progestin) OR (DMPA) OR (Testosterone enanthate) OR (Testosterone undecanoate) OR(male hormonal contraception combination)) AND (DMPA to spermatogenic cells) OR(Testosterone and DMPA to germ cell development)). After initial searching from databases andindividual journal website, 16 scientific articles or journals selected for the review. Result: Theresult indicated that injection of TE and DMPA had suppressed spermatogenesis, might notsuppressed sperm production for a longer period but the decreasing in type B spermatogoniauntil pachytene spermatocyte did occur. On the other hand, injection of TU and DMPA couldmaintain the suppression of sperm production for a longer period due to sustainable higher serumMPA with combination to even longer-acting TU would have more profound effect for that.Conclusion: It was known that combination of TU and DMPA might have better suppression onsperm production than TE and DMPA, otherwise further research needs to be obtained from thecombination of TU and DMPA on spermatogenic development and the association withintratesticular testosterone.
The Effectiveness of Ethanol Extract of Neem Leaf (Azadirachta indica) Mouthwash Against The Growth of Streptococcus sp. Muhammad Abdurrahman; Endang Sri Lestari; Tyas Prihatiningsih
Journal of Biomedicine and Translational Research Vol 8, No 2 (2022): August 2022
Publisher : Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v8i2.14732

Abstract

Background: Streptococcus sp. is a bacteria group in the oral cavity that plays a significant role in initiating plaque biofilm formation on teeth. One way to control plaque is gargling with mouthwash. Neem leaves contain antibacterial compounds such as alkaloids, steroids, glycosides, and flavonoids that can be formulated as mouthwash ingredients.Objective: The study aimed to determine the effectiveness of Ethanol Extract of Neem Leaf (EENL) mouthwash on the growth of Streptococcus sp.Methods: The research design was a pretest-posttest control group. The 28 samples of Streptococcus sp. were divided into 7 treatment groups (4 repetitions). The treatment group was given EENL mouthwash with concentrations of 2.5%, 5%, 7.5%, 10%, and 12.5%, a negative control was given aquadest and positive control was given 0.2% chlorhexidine gluconate. Bacterial growth is known through the absorbance values. Data analysis used one-way Anova test and post hoc LSD test at p<0.05Results: EENL mouthwash has antibacterial activity against Streptococcus sp. One way ANOVA test on the difference in absorbance values shows a p=0.00. LSD post hoc test between EENL concentration of 2.5% and chlorhexidine 0.2% showed equivalent antibacterial activity (p>0.05). Meanwhile, the post hoc LSD test between concentrations of 5% - 12.5% with 0.2% chlorhexidine showed a significant difference in antibacterial power (p<0.05). This indicates a stronger antibacterial activity at a concentration of 5% - 12.5% compared to 0.2% chlorhexidine.Conclusion: EENL mouthwash effective against the growth of Streptococcus sp
Negative correlation of Modified Sperm Stress Test with Leukocyte in Sperm but not with the sperm concentration and motility Dicky Moch Rizal; Geraldo Laurus; Nandia Septiyorini
Journal of Biomedicine and Translational Research Vol 8, No 2 (2022): August 2022
Publisher : Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v8i2.15222

Abstract

Background: Sperm analysis is an initial detection procedure of male infertility recommended by the WHO. Sperm motility as one of the important parameters in sperm analysis has a close correlation with various determinants of male infertility and the success of Assissted Reproductive Technology (ART). Several laboratory assays were carried out as a complement to sperm analysis to describe the sperm ability for fertilization. One of the assays was the modified sperm stress test (MOST) to predict oocyte fertilization in In-Vitro Fertilization (IVF).Objective: This study aimed to assess the correlation between MOST ratio and sperm parameters in infertile men.Methods: This study is a descriptive-analytic study by taking sperm analysis and MOST data from 864 males with infertility problems who went to an infertility clinic, Sadewa IVF, Yogyakarta, Indonesia from May 2015 to November 2020.Results: The mean sperm volume and concentration of the 864 patients were 3.04 mL and 35.74 x 106 / mL respectively. The mean forward motility and normal morphology were 47.93% and 2.07% respectively. The mean leukocyte cell number was 0.84%, and the mean MOST ratio was 0.51. The results showed a positive correlation between sperm concentration (r = 0.235) and motility (r = 0.302) with the MOST ratio. Meanwhile, the leukocyte parameter negatively correlated with the MOST ratio (r = -0.076) and no correlation between other sperm parameters to the MOST ratio.Conclusion:There is a positive correlation between sperm concentration and motility with the MOST ratio, and a negative correlation between the number of leukocytes and the MOST ratio. This result supports the findings of previous studies regarding the importance of sperm motility examination in the success of ART. To the best of our knowledge, this is the first study to assess the MOST in male infertility patients with the big sample size.
The Effect of Duration of Administration and Discontinuation of Fluorometholone Eye Drops on The Trabecular Meshwork Sandi Muslim; Arnila Novitasari Saubig; Riski Prihatningtias; A. Kentar Arimadyo Sulakso; Fifin Luthfia Rahmi
Journal of Biomedicine and Translational Research Vol 8, No 2 (2022): August 2022
Publisher : Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v8i2.13817

Abstract

Background: The extracellular matrix (ECM) in the trabecular meshwork (TM) may play a role in the steroid-induced aqueous humor outflow resistance pathophysiology. Topical steroids such as fluorometholone can cause elevated intraocular pressure. Increased synthesis and accumulation of TM ECM proteins due to steroids result in TM dysfunction and elevated intraocular pressure.Purpose: This study aims to analyze the effect of duration of administration and discontinuation of fluorometholone eye drops on the thickness of the TM ECM of Wistar rats.Methods: This study was an experimental study with a posttest-only control group design. The total sample of 25 rats was divided into 5 groups: Treatment 1 was given topical fluorometholone for 4 weeks and stopped for 4 weeks. Treatment 2 was given fluorometholone for 6 weeks and stopped for 4 weeks. Control 1 was given topical fluorometholone for 4 weeks, Control 2 was given topical fluorometholone for 6 weeks, and the negative control was used as a baseline. TM ECM examination was assessed using histopathological score grading. This study used the Mann-Whitney test and the Kruskal-Wallis comparative hypothesis test.Results: The difference in ECM thickness was not statistically significant either between treatment group 1 and treatment group 2 (p=1.000) or control group 1 (p=0,1000). There was a significant change in the thickness of the TM ECM in treatment group 1, treatment 2, control 1, control 2 compared to the negative control (p=0.003, p=0.003, p=0.003, p=0.004).Conclusion: TM ECM thickness did not return to normal thickness after administration of fluorometholone eye drops one drop 4 times a day for 4 weeks and 6 weeks followed by discontinuation of the drug for 4 weeks. The thickness of the TM ECM was thicker in experimental rats with a longer duration of administration of fluorometholone eye drops.
Correlation between Interleukin-17 Levels with C-Reactive Protein and Neutrophil Lymphocyte Ratio in Sepsis Dwi Retnoningrum; Muchlis Achsan Udji Sofro; Setyo Gundi Pramudo; Satrio Adi Wicaksono; Andaru Dahesihdewi
Journal of Biomedicine and Translational Research Vol 8, No 2 (2022): August 2022
Publisher : Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v8i2.15323

Abstract

Background: Sepsis is a condition in systemic infection associated with organdysfunction. Interleukin-17 is a pro-inflammatory cytokine produced by Th-17 cells. C-reactive protein and neutrophil-lymphocyte ratio (NLR) have been widely used asmarkers of inflammation. The relationship between IL-17 as a proinflammatorycytokine with CRP and NLR has not been reported.Objective: This study aims to prove the correlation between IL-17 with CRP and NLRin septic patients.Methods: Analytical observational study with a cross-sectional approach was carriedout on 40 septic patients in July–December 2020. IL-17 levels were obtained by theELISA method while CRP levels were obtained using the immunoturbidimetric method.NLR was the result of dividing the absolute number of neutrophils and lymphocytesfrom the automatic hematology analyzer. Correlation between variables was performedusing Spearman correlation test.Results: The median levels of IL-17, CPR, and NLR were 363.55 (11.4-1695.80)pg/mL, 13.25 (0.43-53.87) mg/L, and 12.00 (2.26–48.5), respectively. The Spearmancorrelation test between IL-17 levels and CRP obtained p = 0.019, r = 0.37, and NLR p= 0.425, r = 0.13.Conclusion: There is a weak positive correlation between IL-17 levels and CRP inseptic patients. There is no correlation between IL-17 levels and NLR in septic patients.
Thyroid Profile of Childhood Tuberculosis Treated with Anti Tuberculosis Drug During Intensive Phase Moh Syarofil Anam; Mahmudah Mahmudah; Ferdy K Cayami; Maria Mexitalia; Magdalena Sidhartani; Hertanto Wahyu Subagio; Agustini Utari
Journal of Biomedicine and Translational Research Vol 8, No 3 (2022): December 2022
Publisher : Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v8i3.15664

Abstract

Background: Tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis.  The incidence is  10 % in children. Tuberculosis has high morbidity and mortality which needs adequate treatment. The side effects of FDC is thyroid hormone dysfunction which can interfere children's quality of life. However, research about the effect of FDC therapy on thyroid hormone profile in children with TB are very limited. Objective: To determine the effect of anti-tuberculosis drug treatment on thyroid hormone profile in paediatric tuberculosis. Methods: A one group pretest-posttest design study of 50 patients was conducted at the Semarang Health Center for the period January 2021-June 2021. Thyroid function tests of TSH, FT4 and T3, in TB children were measured before FDC therapy and 2 months after administration. The data were analysed descriptively and a comparative test was performed using SPSS 25.Results: There were no significant differences in TSH, FT4 and T3 serum levels before FDC therapy and 2 months of FDC administration. Thyroid hormone levels before treatment and 2 months after administration of FDC, respectively, euthyroid in 44 vs 42 patients.Conclusion: Two months of tuberculosis treatment in children decreased the serum FT4 and T3 level but no changes in the serum TSH level before and after the treatment.
Cigarette Smoke Exposure, but Not High Fat Diet, is Able to Induce Atherosclerosis in Wild-Type Rats Qorry Amanda; Nyoman Suci Widyastiti; Yan Wisnu Prajoko; Nani Maharani; Udin Bahrudin
Journal of Biomedicine and Translational Research Vol 8, No 3 (2022): December 2022
Publisher : Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v8i3.15744

Abstract

Background: High-fat diet (HFD) and cigarette smoke exposure (CSE) have been used to induce atherosclerosis in wild-type (WT) rats however, their effectivity remains unclear.Objective: To confirm and compare the effectivity of HFD and CSE on both the histopathology of aorta and the level of malondialdehyde (MDA) in WT rats.Methods: Thirty-six WT Wistar rats were divided into four main groups (K0, K1, K2, and K3) and four subgroups (K3A, K3B, K3C, and K3D). The negative control group (K0) was fed with regular diet. Group K1 was treated with an intravenous adrenaline followed by high-fat diet (HFD), K2 was fed with regular diet and given CSE, while K3 was treated with a combination of CSE and HFD. The serum and cardiac MDA levels were measured using ELISA. Hematoxylin eosin and oil red O staining of aorta were done for measuring of the intima-media thickness (IMT) ratio and for counting of foam cells, respectively. Results: Both serum and cardiac tissue MDA levels in either K1, K2, or K3 were significant higher (p<0.01) than that of in K0. IMT ratio in K3 was significant higher compared to other groups (p<0.01). Foam cell numbers were significant higher in K2 and K3 groups than that of in either K0 or K1 (p<0.01).Conclusion: While the HFD fails to induce atherosclerosis in WT rats for 28 days, either CSE or combination of CSE and HFD is able to induce it, and the combination is better than alone. 
Rationality of Use and Effectiveness of Stress Ulcer Prophylaxis in Critically Ill Patients: An Experience from A Tertiary Intensive Care Unit Hery Djagat Purnomo; Heru Prabowo; Cecilia Oktaria Permatadewi; Hesti Triwahyu Hutami; Didik Indiarso; Agung Prasetyo; Hirlan Hirlan
Journal of Biomedicine and Translational Research Vol 8, No 3 (2022): December 2022
Publisher : Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v8i3.16387

Abstract

Background: Stress-related mucosal disease (SRMD) frequently develops in critically ill patients, increasing mortality and length of intensive care unit (ICU) stay. There is limited data on stress ulcer prophylaxis (SUP) on critically ill patients in Indonesia.Objective:Methods: This is a retrospective cohort study of patients admitted to the ICU from January 1, 2015, to December 31, 2017. The subjects were all ICU patients who used proton pump inhibitor (PPI) and histamine-2 receptor antagonist (H2RA) as SUP therapy.Results: A total of 315 patients were included, and mean age was 48 years. Approximately 55.2% were women, and 62.8% of patients were admitted to ICU following high-risk surgery. PPI was given to 187 patients (59.4%) and H2RA to 128 patients (40.6%), with an average usage for 5 days. The incidence of SRMD was 15.9% (n = 50), and mean length of stay in the ICU was 6 days. Gender, age, duration of SUP, and ICU length of stay of the PPI and H2RA groups were not statistically different (p > 0.05) and did not affect the GI event (p > 0.05). The use of rational SUP was 98.4%. Major American Society of Health-System Pharmacists (ASHP) risk factor criterion was ventilator use (86.8%), while the minor ASHP criterion was anticoagulant therapy (22.2%). The incidence of GI event was significantly lower in PPI groups than H2RA groups (p < 0.05).Conclusions: The use of SUP is rational with an average use of 5 days. PPI are superior to H2RA for SRMD prophylaxis.
The Protective Effect of N-Acetylcysteine and Honey Against Lungs Damage of Mice (Mus Musculus) After Cigarette Smoke Exposure: A Histological Study Yudhie Djuhastidar Tando; Yuniasih Mulyani Jubeline Taihuttu; Parningotan Yosi Silalahi; Zwesty Anggreany Salhuteru
Journal of Biomedicine and Translational Research Vol 8, No 3 (2022): December 2022
Publisher : Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v8i3.13982

Abstract

Background: Cigarette smoke is a source of free radicals that could cause lung damage. Honey and N-Acetylcysteine have antioxidant effects that could play a role in preventing lung damage.Objective: This study aims to compare the effects of honey and N-Acetylcysteine on lung histological features of mice (Mus musculus) after exposure to cigarette smoke.Methods: This research is an actual experimental study with post-test only control group design. There were 25 adult male mice selected and divided into four groups, i.e., the standard control group (not given exposure or treatment); the negative control group (received 0.2 mL of aqua bidest and exposure to cigarette smoke); the first treatment group (received 0.2 ml of honey and exposure to cigarette smoke); the second treatment  group (received 0.2 mL of N-Acetylcysteine and exposure to cigarette smoke). The treatment was carried out every day for 21 days.Result: One way ANOVA statistical test showed significant differences between the experimental groups (p<0.001). Mice in the group that was only received aqua bidest (negative control group) had the highest percentage of lung damage (76.6%) compared to mice from other groups. The lowest percentage of lung damage was found in the treatment group that received NAC (26.6%). Tukey test showed no significant differences in the lung damage between honey and N-Acetylcysteine groups (p=0.685).Conclusion: Honey and N-Acetylcysteine demonstrated some protective effect against lung damage of mice as a result of cigarette smoking exposure. Further research is recommended by extending the treatment time, increasing the exposure to cigarettes smokes per day, as well as varying the dose of honey and N-Acetylcysteine administered

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