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Journal of Biomedicine and Translational Research
Published by Universitas Diponegoro
ISSN : -     EISSN : 25032178     DOI : -
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology
Journal of Biomedicine and Translational Research (JBTR) is an open access, international peer-reviewed journal that considers articles on: clinical medicine, molecular medicine, tropical medicine, infectious diseases, cardiovascular medicine, molecular biology, genetics, immunology, microbiology, biochemistry, and pharmacotherapy with particular interest on the link between clinical and basic research called translational research.
Arjuna Subject : -
Articles 173 Documents
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Cervical Leiomyoma in Pre-Menopausal Woman: A Case Report Hanif Reza; Nadila Arisa Andriani; Saphira Ayu Suwantari
Journal of Biomedicine and Translational Research Vol 8, No 3 (2022): December 2022
Publisher : Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v8i3.16046

Abstract

ABSTRACT Background: The most common pelvic tumors in women of reproductive age are uterine fibroids or known as leiomyomas, the frequency of cervical leiomyomas is about 1 to 2%. Leiomyoma which causes symptoms requires surgery, the most frequent surgical procedure is hysterectomy. Nevertheless, the management of cervical leiomyomas in premenopausal women remains a challenge in order to preserve the uterus whereas future pregnancy is still possible. Therefore, hysterectomy is not the preferred procedure. We present a case of a 47-year-old, multiparous woman with cervical leiomyoma that was treated with myomectomy and curettage to give an overview of how to treat cervical leiomyoma in patients who wanted to preserve their fertility.Case Presentation: A 47-year-old woman, P3A0, presented to the emergency department at Sultan Agung Islamic Hospital with a mass spontaneously protruding from the vagina a few hours before admission with a mass spontaneously protruding from the vagina, lower abdominal and heavy vaginal bleeding. The gynecological examination revealed a mass with a reddish surface and blood protruding out of the introitus. The dimensions of the mass were 20 cm x 8 cm x 12 cm. The mass is mobile, painful, tender on palpation, and seemed to be originating from the cervix. Myomectomy and curettage were performed. The histopathology result was suggestive of benign leiomyoma.Conclusion: In conclusion, this was a case of a patient with symptoms associated with cervical leiomyoma who was effectively treated with myomectomy and curettage. Myomectomy is considered the most frequent therapy for symptomatic myomas for women who desire to preserve their fertility.
Hepatogomax Improves Serum Albumin and Transaminase Enzyme Activity Levels in Sprague Dawley Rats Liver Cirrhosis Refani Alycia Kusuma; Etika Ratna Noer; Hery Djagat Purnomo; Adriyan Pramono; Endang Mahati; Choirun Nissa
Journal of Biomedicine and Translational Research Vol 8, No 3 (2022): December 2022
Publisher : Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v8i3.15078

Abstract

ABSTRACTBackground: Patients with liver cirrhosis had liver cell damage and malnutrition risk. Hepatogomax enteral formula consists of soybean flour and goat’s milk flour which could reduce serum albumin, AST, and ALT levels because it contains BCAA amino acids (valine, leucine, and isoleucine) and MCT.Objective: To examine the effect of Hepatogomax enteral formula based on Soybean flour and goat’s milk flour on serum albumin, AST, and ALT levels in Sprague Dawley rats liver cirrhosis.Methods: The true experimental study – pre-post control group used 24 adult Sprague Dawley rats in 4 groups. Groups K(+), P1, and P2 were induced by Thioacetamide 400 mg/kgBW. The groups of  P1 and P2 were given the Hepatogomax enteral formula made from soybean flour and goat’s milk flour at a dose of 4.87 g/200gBW and 14.6 g/200gBW for 28 days. Serum albumin levels were determined using the Bromocresol Green (BCG) method while serum AST and ALT were determined using spectrophotometry. Statistical analysis had used Paired T-Test and Kruskal Wallis test with Mann Whitney follow-up test.Results: Serum albumin, AST, and ALT levels in the P1 and P2 groups had significant differences (p<0.05) against the K(-) and K(+) groups. Enteral formula based on soybean flour and goat’s milk flour could increase serum albumin levels and reduce serum AST and ALT levels of P1 and P2 groups. The most significant improvement in serum albumin, AST, and ALT levels was at dose 14.6 g/200gBW.Conclusion: Hepatogomax enteral formula based on Soybean flour and goat’s milk flour could increase serum albumin and redue AST, and ALT levels Sprague Dawley rats liver cirrhosis.
Genetic Counseling in a Couple with Primary Infertility Peter Ivan Hadiprajitno; Sultana Faradz
Journal of Biomedicine and Translational Research Vol 8, No 3 (2022): December 2022
Publisher : Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v8i3.16179

Abstract

Background: Couples unable to conceive and bear children could feel deep disappointment, often leading to depression. Infertility is one of the main reasons couples are not able to have children. Genetic counseling role in infertility ranges from explanation about possible genetic causes of infertility, pregnancy planning, and advice for treatment. Case Presentation: A couple with 16 years of infertility was referred to the genetic clinic at National Diponegoro Hospital. The 42 years old female had previous history of diabetes mellitus, obesity, and had treatment of epilepsy/seizure 15 years ago with routine carbamazepine therapy for 2 years, while her 42 years old husband had active hepatitis B infection for 15 years. This couple underwent insemination program twice and once completed In Vitro Fertilization (IVF), both management bearing no successful implantation or viable pregnancy. Recently, she underwent a laparoscopy procedure, which gave new diagnosis of endometriosis and adenomyosis. Chromosomal examination and Methylenetetrahydrofolate Reductase (MTHFR) C677T and A1298C alleles analysis were done in our laboratory. Both individuals carried normal karyotypes and MTHFR analysis was homozygote wild type allele. Currently, this couple has accepted their conditions. They still want to bear a child although she is at a crucial ageConclusion: Infertility is a challenging and comprehensive problem. As healthcare professionals, we encounter problems not only in diagnosis and management, but also psychological and emotional dilemma. Genetic counseling is needed to solve the problems and avoid patient’s psychological distress.
The Effect of Roselle Flower Infusion (Hibiscus sabdariffa) on Retinal Ganglion Cell Apoptosis of Sprague Dawley Rats Exposed to Cigarette Smoke Riski Prihatningtias; Puspita Kusuma Dewi; Desy Armalina; Hermawan Istiadi; Rahma Athifah Amelia; Faizah Fulyani
Journal of Biomedicine and Translational Research Vol 8, No 3 (2022): December 2022
Publisher : Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v8i3.15442

Abstract

Background: Cigarette smoke contains a lot of free radicals that can reduce antioxidants in the body. One of those is Reactive Oxygen Species (ROS) which can induce Retinal Ganglion Cell (RGC) apoptosis. Reduced ganglion cell axons will eventually lead to optic neuropathy. Supplementing herbal medicines, vitamins, foods, and drinks containing antioxidants is one way to increase oxidant levels in the body. Roselle or Hibiscus sabdariffa is a herbal plant reported to have a lot of antioxidant content.Objective: The study aimed to prove that Roselle flower infusions can reduce retinal ganglion cell apoptosis of Sprague Dawley rats exposed to cigarette smoke.Methods: This was an experimental study with a post-test-only design with a total of 24 male Sprague Dawley rats exposed to four non-filtered cigarettes/day for 30 consecutive days. They were randomly divided into four sub-groups, and each group consisted of 6 rats. The control group received 1 ml of saline, and three treatment groups received Roselle flower infusions of 2.5%, 5%, and 10% for 30 days. Enucleated eyes were then made paraffin blocks and slides with HE staining. The retinal ganglion cell apoptosis score was assessed by calculating the number of apoptotic cells using 1000x in five fields of view. The data were analyzed using Kruskal-Wallis non-parametric test followed by the Mann-Whitney U test.Results: The average retinal ganglion cell apoptosis score in control, treatment 1, 2, and 3 groups were 3.00; 2.83; 2.00; 1.83. There was a significant difference between the control group and treatment groups 2 (p=0.001) and group 3 (p=0.001), while between the control group and treatment group 1, there was no significant difference (p=0.317).Conclusion: The administration of 5% and 10% Roselle flower infusions can reduce the apoptosis score of retinal ganglion cells in Sprague Dawley rats exposed to cigarette smoke. The higher the Roselle flower infusion concentration, the lower the apoptosis score of retinal ganglion cells.
The Effect of Liprotide-Encapsulated Vitamin D3 on MDA and SOD in Rats Deficient Vitamin D and Calcium Untari Untari; Gemala Anjani; Faizah Fulyani; Adriyan Pramono; Endang Mahati; Sylvia Rahmi Putri; Reza Achmad Maulana
Journal of Biomedicine and Translational Research Vol 9, No 1 (2023): April 2023
Publisher : Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v9i1.16289

Abstract

Background: Vitamin D deficiency is frequently correlated with elevated malondialdehyde (MDA) levels and decreased superoxide dismutase (SOD) activity. Several studies have demonstrated that vitamin D3 can reverse intracellular oxidative stress. However, vitamin D is prone to deterioration and instability. Liprotides contain lipids and proteins that can prevent vitamin D from oxidating.Objective: This study aims to investigate the effects of liprotide-encapsulated vitamin D3 on MDA concentrations and SOD activity in calcium and vitamin D-deficient rat models.Methods: The experimental post-test-only control group study used 24 Wistar rats randomly in 4 groups. Groups K(-), K(+), and P were fed a vitamin D and calcium-depleted AIN-93M diet for 14 days. Standard feed AIN-93M was received by normal groups (KN). Groups K- were deficient rats in vitamin D and calcium without intervention. The groups of  K+ and P were given vitamin D3 (180 IU) which was non-encapsulated and liprotide-encapsulated for 28 days.The SOD activity was quantified with Superoxide Dismutase (SOD) Activity Assay Kit, while MDA levels were determined using Thiobarbituric Acid Reactive Substance (TBARS) method. The statistical analysis used One-way ANOVA test with Least Significant Difference follow-up test.Results: The MDA levels and SOD activity in the K+ and P groups had significant differences (p<0.05) against the control group. Liprotides-encapsulated vitamin D3 significantly reduced MDA levels and enhanced SOD activity compared to non-encapsulated in rats with a deficiency in vitamin D and calcium.Conclusion: Liprotide-encapsulated vitamin D3 has the potential to increase SOD activity and decrease MDA levels. 
Renoprotective Effect Of Sambiloto (Andrographis Paniculata) Leaf Extract On Lipopolysaccharide – Induced Septic Rats Adhika Bastian Bagas Prananta; Nyoman Suci Widyastiti; Ariosta Ariosta; Dwi Retnoningrum; Rezya Salsabela; Vega Karlowee; Neni Susilaningsih
Journal of Biomedicine and Translational Research Vol 9, No 1 (2023): April 2023
Publisher : Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v9i1.17286

Abstract

Background: Associated with sepsis is the illness known as acute kidney injury (AKI). AKI may be mediated by uric acid, and blood creatinine levels can be utilized to diagnose the condition to measure kidney function. Sambiloto (Andrographis paniculata) is a traditional medicine that has flavonoid compounds that can reduce creatinine levels and Xanthine Oxidase Inhibitors which can reduce uric acid levels.Objective: Septic model rats generated by lipopolysaccharide were used to test the effects of sambiloto (Andrographis paniculata) leaf extract on serum creatinine and uric acid levels (LPS).Methods: This study was experimental employing 25 rats split into 5 groups as the post-test alone control group: healthy control with standard feed (HC), negative control with LPS injection(NC), Treatment (T)1 (A.Paniculata 200 mg/kgBW+LPS), T2(A.paniculata 400 mg/kgBW+LPS), and T3(A.paniculata 500mg/kgBW+ LPS). A.paniculata leaf extract was given via oral gavage on day 8-21. Intraperitoneal injection of LPS 5 mg/kgBW was given on day 22. On the 25th day, the blood serum was analyzed for creatinine levels using Jaffe method and uric acid was analyzed using the enzymatic photometric method. One-way analysis of variance (Kruskal-Wallis) and the Kruskal-Wallis test were used to evaluate the data.Results: The mean creatinine levels of HC,NC,T1,T2,T3 were 0.7±0.01;3.5±0.04;2.9±0.03;1.9±0.05; 1.3 ±0.04 mg/dl respectively. The mean uric acid levels of HC.NC.T1.T2.T3 were 1.7±0.05;8.2±0.11; 4.5±0.03;4.0±0.12;3.0±0.19 mg/dl respectively. There were significant differences (p<0.05) in creatinine levels in groups T2(p=0.031) and T3(p=0.001) to NC group and serum uric acid levels in groups T1(p<0.001), T2(p<0.001), and T3(p<0.001) to NC group which creatinine and uric acid levels were lower than NC group.Conclusion: Andrographis paniculata leaf extract has renoprotective effect against AKI in LPS-induced septic ratsKeywords: Andrographis paniculata, creatinine, uric acid, lipopolysaccharide, AKI, sepsis
Effects of Fermented Tempeh Using Rhizopus oligosporus and Lactobacillus rhamnosus GG on Body Weight, Lee Index, High Sensitivity C Reactive Protein and Lipid Profile of Obese Rats Fatih Az Zahra; Faizah Fulyani; Nani Maharani; Gemala Anjani; Etika Ratna Noer
Journal of Biomedicine and Translational Research Vol 9, No 1 (2023): April 2023
Publisher : Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v9i1.17381

Abstract

Background: Tempeh is a fermented soybean containing isoflavones that shows good benefits again obesity. Co-fermentation of tempeh using  Lactobacillus rhamnosus GG could increase the bioavailability of isoflavones.Objective: This study aimed to determine the effect of co-fermented tempeh using Lactobacillus rhamnosus GG (tLGG) on body weight (B.W.), Lee Index, high sensitivity C Reactive Protein (hs-CRP), and lipid profile of obese rats. Total flavonoid and genistein were also measured.Methods: Male Sprague Dawley rats (n=36, 200-to-215-gram, age eight weeks) were orally administered high fat and high sucrose diet (HFHS diet) for two weeks to induce obesity. After obesity was confirmed by checking Lee Index, rats were divided into six group and administered orally standard diet (normal control), HFHS diet (negative control), HFHS diet and 120 mg/kg B.W./day-orlistat (positive control), HFHS diet and 60 mg/kg B.W./day standard tempeh  with Rhizopus oligosporus (tS), HFHS diet and 60 mg/kg B.W./day tLGG, HFHS diet and 120 mg/kg B.W./day tLGG for four weeks. Body weight, Lee Index, hs-CRP, and lipid profile were analyzed. Total flavonoid and genistein were analyzed.Results: 120 mg/kg B.W./day tLGG group exhibited significantly lower body weight gains, Lee Index, hs-CRP, triglyceride, total cholesterol, LDL, and higher HDL compared to negative control and tS group (p<0,001), however, positive control group exhibited lower body weight gains compared to tLGG group (p<0,001). Nevertheless, tLGG group exhibited lower Lee Index compared to positive control group.   tLGG showed higher flavonoid and genistein level than tS.Conclusion: Administration of 120 mg/kg B.W./day tLGG showed significantly lower Lee Index compared to all groups given HFHS diet, however, positive control group showed lower body weight gains compared to tLGG group. tLGG also improved hs-CRP and lipid profile two times better than negative control group. tLGG increased total flavonoids and genistein level.
Effect of Probiotic Supplementation on FSH, LH Levels and Folliculogenesis Nur Rahman; Syarief Thaufik Hidayat; Besari Adi Pramono; Raden Soerjo Hadijono; Agoes Oerip Poerwoko; Arufiadi Anityo Mochtar
Journal of Biomedicine and Translational Research Vol 9, No 1 (2023): April 2023
Publisher : Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v9i1.16824

Abstract

Background: Polycystic ovary syndrome (PCOS) is an endocrine disorder that often occurs in women of reproductive age. The main therapy currently used to treat PCOS patients is insulin sensitizer. In PCOS, there is an imbalance in the intestinal flora which causes activation of the immune system and an inflammatory response that leads to insulin resistance. The effect of probiotic supplementation on insulin resistance can have an impact on changes in reproductive hormone levels in PCOS women. Objective: Analyze the effect of probiotic supplementation on levels of FSH, LH, and folliculogenesis in a study of PCOS Wistar rats. Methods: Experimental research with a post-test only controlled group design. The research sample was 35 Wistar rats which were divided into 5 groups, (K-) were healthy rats, (K+) were PCOS rats without treatment, (P1) were PCOS rats received Metformin, (P2) were PCOS rats received Probiotics, and (P3) were PCOS rats that received Metformin + Probiotics. The intervention was carried out for 14 days. The dependent variables were levels of FSH, LH and folliculogenesis. Research data were analyzed using one-way ANOVA test, Fisher Exact test. Differences between groups and controls were tested with Dunnett's post hoc test. Significant p<0.05Results: Metformin + probiotic supplementation resulted in increased FSH levels, decreased LH levels and increased folliculogenesis when compared to the PCOS rats without treatment (K+).Conclusion: Metformin + probiotic supplementation causes levels of FSH, LH and folliculogenesis activity in PCOS rats to resemble healthy rats.
Understanding Platelet-Rich Plasma as Potential Therapy To Improve Cardiac Function After Myocardial Infarction: Based On Evidence Linda Chiuman; Dearni Anggita Krismayani Purba; Hendy Million Samin
Journal of Biomedicine and Translational Research Vol 9, No 1 (2023): April 2023
Publisher : Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v9i1.17379

Abstract

Myocardial infarction is the leading cause of death in the world’s population. The sudden and progressive loss of cardiomyocyte contractile cells due to infarction is disproportionate to the number of new cells formation. This pathological condition will ultimately reduce the function of the heart muscles. Platelet-rich plasma (PRP) in the last decade has been attracting attention regarding its role in cell regeneration. The content of cytokines and growth factors contained in PRP such as Platelet-Derived Growth Factor (PDGF), Transforming Growth Factor Beta 1 (TGF-Β1), Epidermal Growth Factor (EGF), Vascular Endothelial Growth Factor (VEGF), Basic Fibroblast Growth Factor (bFGF) and others are known to be involved in the migration, proliferation, and differentiation of various cell types and to induce post-ischemic angiogenesis. This review aims to analyze and investigate the benefits of PRP therapy in myocardial infarction. Recent literatures regarding the use of PRP in myocardial infarction was collected by searching PubMed and Google Scholar databases with the keywords of “Platelet-rich plasma”, “Myocardial Infarction”, “Reperfusion”, and “Angiogenesis”. The researches used was published between January 2011 and December 2022. Based on the results, Platelet-rich Plasma (PRP) is considered capable of accelerating angiogenesis, mitogenesis, protect cells from free radicals, reduce infarct area and scar tissue in myocardial infarction. PRP can also improve cardiac function in post myocardial infarction by increasing ejection fraction. In addition, PRP can also reduce treatment costs due to the risk of myocardial infarction complications such as bleeding and infection.
Potential of B-Cell Epitopes Protein Ag85 Complex Mycobacterium Tuberculosis as Serodiagnostic Antigen of Tuberculosis by in Silico Study Diana Chusna Mufida; Ayu Munawaroh Aziz; Nurrul Izza Misturiansyah
Journal of Biomedicine and Translational Research Vol 9, No 1 (2023): April 2023
Publisher : Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v9i1.16379

Abstract

Background: The high case of tuberculosis which isn't followed by good detection becomes an urgency for the diagnostic developments. One of them with immunodiagnostic principle uses B-cell Ag85 complex epitope. The design of the diagnostic epitope was performed by mapping the B cell epitope used in silico studies.Objective: The purpose of this research is to analyze antigenicity, physicochemical which affect immunogenicity, and homology of B-cell Ag85 complex epitope with the strain which circulates in Indonesia.  Methods: The samples used were taken from the NCBI protein bank with access numbers P9WQP3 for Ag85A, P9WQP1 for Ag85B, and P9WQN9 for Ag85C. The sequences were analyzed using IEDB (Bepipred) software as the epitope prediction, VaxiJen as antigenicity prediction, ProtParam as physicochemical properties prediction, and BLASTP NCBI as sequence alignment.Results: Twenty seven epitopes were antigenic with 0.4297 to 2.6007 scores and the molecular weight was from 619.59 Da to 3145.36 Da. This research also obtained eleven stable and hydrophilic epitopes. The alignment of 11 candidate epitopes with the strain which circulates in Indonesia, had a similarity percentage of 85.71%-100% and 3 epitopes had a more significant score.Conclusion: Three epitopes of Ag85 complex; Ag85A (212-235), Ag85B (209-237), and Ag85C (283-310), were universal antigens and can be developed into diagnostic antigens in Indonesia. 

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