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Proceeding International Conference on Global Resource Conservation
Published by Universitas Brawijaya
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Articles 37 Documents
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Search results for , issue " Vol 6, No 1: Proceeding of 6th ICGRC 2015" : 37 Documents clear
Exploration and Morphological Character Identification of Local Durian (Durio zibethinus Murr.) from Tidore Island, North Maluku Sundari, Sundari; Arumingtyas, Estri Laras; Hakim, Luchman; Azrianingsih, Rodiyati
Proceeding International Conference on Global Resource Conservation Vol 6, No 1: Proceeding of 6th ICGRC 2015
Publisher : Proceeding International Conference on Global Resource Conservation

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Abstract

Local durian is the name for Durio zibethinus Murr. found in the Tidore Island, North Maluku. There are many local names for local durian in Tidore Island that have many different morphological variation. The objective of this study was to inventarize and analyze the morphological charactervariations of 19 local durian names collected from 9 villages: Gurabunga, Gubukusuma, Afa-afa, Fobaharu, Foralaha, Jaya, Bobo, Dokiri and Rum in the Tidore Island, North Maluku. This research was conducted in March-June 2015 using descriptive exploratory method with purposive sampling. Local durians found in Tidore Island have variations local names and variation of morphological characters include: habitus, leaf shape, fruit shape, flesh color and texture, seedshape, and the shape ofthe aril thorn. Keywords: inventarization; local durian; morphological characters; Tidore Islands
A review of Vertical and Spatial Variations of Canopy Insects in Secondary Forests in Central Japan and East Java, Indonesia Leksono, Amin Setyo; Nakagoshi, Nobukazu
Proceeding International Conference on Global Resource Conservation Vol 6, No 1: Proceeding of 6th ICGRC 2015
Publisher : Proceeding International Conference on Global Resource Conservation

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Abstract

The vertical and spatial variations in the abundance of canopy insects werecompared between secondary forests in Kanazawa, Japan and Trawas, East Java, Indonesia. The patterns of canopy insect abundance and composition change with layer and site weredetermined. The study in Central Japan was conducted at a mosaic habitat of a suburban forest in Kanazawa, Central Japan. Two patches were selected for sampling: a young deciduous patch and a mixed vegetation patch. Canopy insects were collected 12 times from June to October 2003. Another study site is located in Trawas, East Java, Indonesia. Insect sampling was performed at three sites along a gradient of anthropogenic disturbances, from a less-disturbed site in an upper hilly area, through a partly-disturbed site, to a highly-disturbed site. Samplings were performed by using window traps, suspended in the canopy layer and in the understory layer. This study revealed that canopy insect communities vary among canopy layers and sites. However, this study did not indicate consistent insect stratification between regions. Data in temperate region showed that the abundance of canopy insects is greater in the canopy layer than in the understory layer. In tropical region, the abundance of canopy insects between understory and canopy layer was similar. The young deciduous patch consisted of more individuals of canopy insects than in the mixed vegetation patch. Our study in tropical region demonstrated significant effects of disturbance on the abundance and family richness of canopy insects. The overall insect abundances and richness differed among sites.Keywords: Anthropogenic disturbance; canopy insect community; secondary forest; temperate and tropical region; window trap
Potential Uses of Plant in Waru-waru and Teluk Semut Subregion, Sempu Island Rahadiantoro, Apriyono; Danarto, Setyawan Agung
Proceeding International Conference on Global Resource Conservation Vol 6, No 1: Proceeding of 6th ICGRC 2015
Publisher : Proceeding International Conference on Global Resource Conservation

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Abstract

Indonesia is the largest archipelago and one country with high biodiversity. The center of diversity is found on five largest islands include Sumatra, Java, Kalimantan, Sulawesi and Papua. Little is known about the potential use of plant  Small island known susceptibility to the decline of biodiversity. In 2015, Purwodadi Botanic Garden have arranged plant assessnment in Waru-waru and Teluk Semut subregion, Sempu island. There are many plant collection have obtained but potential uses of plant have not been known. The purpose of this study was to know potential use of plant in Waru-waru and Teluk Semut Subregion, Sempu Island. The results showed that Waru-waru and Teluk Semut Subregion, Sempu Island has 100 plant species with potential use as medicine, food, timber, ornamental plants, and industry. Keywords: Potential uses of plant; Purwodadi Botanic Garden; Sempu island; Teluk Semut; Waru-waru
Gonadal Maturity Induction using Karamunting (Melastoma malabatrhicum) Ethanol Extract on White Shrimp Female (Litopenaeus vannamei) Ridwan, Ahmad; Awaludin, Awaludin; Anggraeni, Tjandra
Proceeding International Conference on Global Resource Conservation Vol 6, No 1: Proceeding of 6th ICGRC 2015
Publisher : Proceeding International Conference on Global Resource Conservation

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Abstract

Common problem that happened in white shrimp culturing is insufficiency of mature female to provide the seed simultaneously. There are several methods to accelerate gonadal maturity infemale white shrimp: eye ablation, environmental manipulation, and providing high cholesterol fresh feed. Till now, high cholesterol feed is not very common method. Karamunting or Malabar Melastome (Melasthoma malabatrhicum) is known to have a high cholesterol content, more specifically, lanosterol based on GCMS test. Lanosterol is cholesterol that contained in plants, which assumed as precursor hormone for gonad maturity process in female of white shrimp. The aim of this experiment is to determine whether Karamunting ethanol extract can stimulate and accelerate gonad development in female of white shrimp. This experiment consists of several steps: (1) Karamunting (Melastoma malabathricum) extraction, (2) GCMS test for Karamunting, (3) extract injection to female of white shrimp (Litopenaues vannamel) for 15 days in 3 days interval with dosage variable 0 (control), ), 10 mg/kg BW (P1), 7,5 mg/kg BW (P2), 5 mg/kg BW (P3), 2 mg/kg BW (P4) dan 1 mg/kg BW (P5), (4) The measuring parameters are Karamunting content, morphological gonad development observation and hepatopancreas somatic index (HSI) measurement, (5) Data analysis. Data shows that karamunting ethanol extract with P1 and P2 dosage can result in morphological gonad development response (GML II), meanwhile with P3, P4 and P5 dosage there is no changes observed. All variables didn’t affect the level of HSI (P>0.05). Based on the result, it can be concluded that Karamunting ethanol extract indicates an acceleration of gonad maturity process in white shrimp female.Keywords : Gonad maturity; Hepatopancreas Somatic Index ; L.  vannamei; M. malabatrhicum
Utilising Synthetic Biology to Produce Renewable Energy from Indonesia’s Biodiversity: Inspiration from iGEM Competition Kemal, Rahmat Azhari; Rini, Ika Agus; Oktaviani, Indah
Proceeding International Conference on Global Resource Conservation Vol 6, No 1: Proceeding of 6th ICGRC 2015
Publisher : Proceeding International Conference on Global Resource Conservation

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Abstract

The emerging field of synthetic biology focuses on engineering biomolecular systems and cellular capabilities. It can be applied to a wide variety of applications, one of them is renewable energy production. Teams competing in the International Genetically Engineered Machine (iGEM) competition design and assemble biological devices able to do certain purposes such as producing renewable energy. Their ideas can be adapted by using compatible organisms available from Indonesia’s rich biodiversity. Several ideas are highlighted in this review. One of them is the idea of recycling methanol in glycogen that can be used as carbon source for biofuel production. Methanol dehydrogenase from Indonesia’s methylotrophic bacteria can substitute Bacillus methanolycus used in the system. The adapted ideas can serve as starting points for research, production, and implementation of renewable energy in Indonesia.Keywords: Biodiversity; iGEM; Indonesia; renewable energy; synthetic biology
In silico Docking Reveals Interaction Pattern between Topoisomerase I and Camptothecin Analogs: Implication for Ovarian Cancer Treatment Nuha, Ulin; Mustofa, Zainul
Proceeding International Conference on Global Resource Conservation Vol 6, No 1: Proceeding of 6th ICGRC 2015
Publisher : Proceeding International Conference on Global Resource Conservation

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Abstract

Ovarian cancer is one of cancer types that begins in the ovaries. Many kind of anticancer agents are developed for the treatment now. Camptothecin analogs are agent that inhibit the action of DNA topoisomerase I, a nuclear enzyme that relaxes the supercoiled DNA during the replication, recombination, transcription, and repair of DNA. FDA have been approved two camptothecin analogs for the treatment of ovarian and colorectal cancer, those are topotecan and irinotecan. Another analogs are in various stages of clinical development, including 9-aminocamptothecin, rubitecan, lurtotecan, and exatecan mesylate. The purpose of this study is to discover interaction pattern between topoisomerase I and camptothecin analogs through in silico docking. PyRx is used for DNA topoisomerase I and camptothecin analogs docking. The interactions between topoisomerase I and camptothecin analogs were visualized and analyzed using PyMol and LigPlus. Our results show that the binding pattern between another camptothecin analogs, including rubitecan, lurtotecan, and 9-aminocamptothecin, with DNA topoisomerase I is relative similar with irinotecan-DNA topoisomerase I complex, specifically in the binding of Arg364, Asn491, Lys493 and Lys532.6 Keywords: camptothecin analogs; in silico docking; interaction pattern; ovarian cancer; topoisomerase I
Mapping of Collected Plants Grown in Waru-Waru and Teluk Semut Costal at Sempu Island, Malang, Indonesia Irawanto, Rony; Rahandiantoro, Apriyono; Mudiana, Deden
Proceeding International Conference on Global Resource Conservation Vol 6, No 1: Proceeding of 6th ICGRC 2015
Publisher : Proceeding International Conference on Global Resource Conservation

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Abstract

The Purwodadi Botanic Garden main duty is ex-situ plants conservation, especially from dry lowland area. Conservation can be carried through the exploration and collecting the plants diversity in an priority area The selected area for plants exploration activities in next several years are small islands. Sempu Island is selected a small island. AlsoSempu Island have status as nature reserve with a diversity of ecosystems such as mangrove forests, coastal forests, lowland forests and grasslands, as well as the diversity of flora and fauna that are endemic and unique. Based on some research previously the plants diverisity of Sempu Island are 282 species included in 80 families at 10 blocks location, namely: Telaga Lele, Telaga Sat, Telaga Dowo, Gladakan, Baru-baru, Gua Macan, Teluk Ra’as, Teluk Semut, Air Tawar, and Waru-waru. This study aims to show the mapping of collected plants  from exploration in Waru-Waru and Teluk Semut Coastal in Sempu Island. mainly at two site locations are Waru-Waru and Teluk Semut that separeted into two team. This is done as a basis for planning activities in the exploration and collecting Sempu Island the following year. After conducting flora exploration of the plant collected are 274 numbers (1.106 plants material) with scientific name are 111 species. Some plant were found and collected by every team are same, around 24 species and 21 families.The new plants collection for Purwodadi Botanic Garden are 21 species. The plant collected from exploration of flora are mapping to see coverage exploration area and this picture can give us undestanding the other area could be explore. From result know that exploration flora in 2015 year  still on the coastal around the northern part of the island Sempu.Keywords: Exploration; mapping; plants; Sempu Island 
Molecular Detection of Enterobacteriaceae and Escherichia coli from Water Hilson, Eunike Ilona; Prana, Mertha; Satwika, Dhira
Proceeding International Conference on Global Resource Conservation Vol 6, No 1: Proceeding of 6th ICGRC 2015
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Abstract

Well water has become the important source for public daily activities, especially as drinking water. Enterobacteriaceae is a bacterial group, often found in well water. There are 8 genus of Enterobacteriaceae consider as enteric group because of its relationship to fecal bacteria from human and animal gastrointestine.  Escherichia coli, a member of this group, is one of the best microbiological fecal indicator that have been used for water monitoring. The presence of this bacterial group is of human interest, especially related to their pathogenic characteristic and as the fecal indicator for water quality. This is the objective of this research to evaluate the specivity of new primers which were deduced from partial sequences of rpoB, gyrB and lacZ genes specifically designed for Enterobacteriaceae and E. coli detection. After being optimized by mean of setting the annealing temperature, they are used for in vitro analysis. The result showed the primers are specific enough for Enterobacteriaceae and E. coli detection.Keywords:  Enterobacteriaceae, E. coli, gyrB, lacZ, rpoB
Spatial Distribution of Birds in Coban Trisula, Bromo Tengger Semeru National Park Iqbal, Kemal; Gama, Zulfaidah Penata; Yanuwiadi, Bagyo
Proceeding International Conference on Global Resource Conservation Vol 6, No 1: Proceeding of 6th ICGRC 2015
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Abstract

Bromo Tengger Semeru National Park has vegetation that allows the availability of suitable habitat for various species of birds. Species of birds that can be found in Coban Trisula are very diverse. The purpose of this research was to determine the spatial distribution patterns of birds in Coban Trisula area. This study used point count method. The observation was conducted in February to April 2015. Species data were calculated and presented in the form of tables and figures. The spatial distribution of birds was determined by using an index of dispersion. The results of the study showed that 43 species of birds consists of 6 Order and 17 Family. The spatial distribution result showed that the majority of spatial distribution pattern were in aggregation patterns. In theory, aggregation patterns in species of birds can be concluded that they are sharing foraging area (sharing resources), safe, easy to meet between males and females as well as their natural character.  Keywords: birds; Coban Trisula; spatial distribution
The Capability of Total Protein Extracted from Black Sea Cucumbers (Holothuria leucospilota) in Agglutinating of Mouse Sperms (Mus musculus) Haryanto, Hery; Ruyani, Aceng; Piramida, Cally
Proceeding International Conference on Global Resource Conservation Vol 6, No 1: Proceeding of 6th ICGRC 2015
Publisher : Proceeding International Conference on Global Resource Conservation

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Abstract

The research aimed to know the effect of total protein extracted from black sea cucumbers (Holothuria leucospilota) on agglutination of mouse sperms (Mus musculus). The flesh of black sea cucumber  was homogenized in Tris Buffer Saline (TBS) at pH 7.4. The homogenate was added with ammonium sulphate 50%(w/v) for presipitating protein. The presipated protein was purified with 12.000 MWCO dialysis bag to remove ammonium sulphate. The purified protein was quantitated with Biuret Method. The concentration of purified protein was 4,97g/dL. There were five concentration proteins used mouse sperm agglutination test, i.e. 4.97, 3.52, 2.48, 1.17 g/dL and 0% (control). Mouse sperm suspension was prepared by soaking an epypydimal duct in 0,9% (w/v) NaCl solution. The purified protein in varied concentration was then used for agglutination of suspension of mouse sperm. This research used Completely Randomized Design (CRD) with 5 concentrations of protein  and 5 replications. The data of this research were analyzed by using ANOVA. The result showed that total protein extract of black sea cucumberwas significantly different on mouse sperm agglutination. The fastest time of agglutination of was indicated by concentrations of  4.97 g/dL. total proten of black sea cucumber. Keywords: Black Sea Cucumber (Holothuria leucospilota); mouse sperm suspension; sperm agglutination; total protein extract

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