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Articles 16 Documents

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Search results for , issue "Vol 2, No 2 (2013)" : 16 Documents clear

BSA Immobilization on QCM Sensor with Hydrophobic-Hydrophilic Properness Modification of Polystyrene Surface Using Ultraviolet (UV) Radiation Fenny Irawati; Setyawan P. Sakti; Unggul P Juswono; Aulanni’am Aulanni’am
Natural B, Journal of Health and Environmental Sciences Vol 2, No 2 (2013)
Publisher : Natural B, Journal of Health and Environmental Sciences

Research has been done to improve the optimization of the BSA (Bovine Serum Albumin) immobilization process on QCM sensor by modifying the nature of the hydrophobic-hydrophilic polystyrene surfaces using ultraviolet radiation (UV). The silver electrode of QCM sensor coated with a solution of polystyrene using spin coating technique. Sensor with polystyrene coating was heated using an oven until it reaches a temperature of 200 °C and then irradiated with ultraviolet light. A 30 µL of distilled water was dropped on the sensor surface and the formed contact angle was observed. The observation of the contact angle showed that there was a change in the nature hydrophobicity of sensor surface irradiated with UV. BSA immobilization was done by injecting a certain amount of BSA concentration in PBS solution on top of the sensor. We found that best surface for immobilization of BSA is one which is without UV irradiation. This means that the immobilization is mainly caused by hydrophobic force.

Variant Clustering Analysis Amorphophallus muelleri Blume Found in East Java Based on Molecular Marking CslA Mannan Synthase Encoder with PCR-RFLP Technique Novie Ary Priyanti; Arik Arubil; Laras Estri Arumningtyas; Rodiyati Azrianingsih
Natural B, Journal of Health and Environmental Sciences Vol 2, No 2 (2013)
Publisher : Natural B, Journal of Health and Environmental Sciences

Amorphophallus muelleri Blume is a ubiquotus plant found all over Indonesia and has the potential to be food substitute due to its high glucose content. This research was conducted to investigate the porang’s clustering based on CslA gene glucose coding by using PCR-RFLP technique. Porangs used as the subjet of this study were obtained from 4 different locations in East Java, wich are : (1) Nganjuk, (2) Madiun, (3) Blitar and (4) Jember, analysed by morphological characteristic and molecular traits. Morphological analysis showed that porang derived based on their stalk pattern. BlitarI, JemberI, MadiunI variant have large prismatic stalk patterns, Jember1 and Nganjuk2 have a tight prismatic stalk pattern. Glucomannan content analysis showed that highest content is from Blitar2 5,469% and the lowest is Madiun2 0,2313%. The amplification of CslA gene sequence using primary AkCslA680F and AkCslA1288R showed that CslA gene sequence was amplified on 200 bp. The cutting by using HaeIII restrictive enzyme produced two monomorphic band on 1000 bp and 2000 bp, and 8 polymorphic band on 100 bp-400 bp. The result of this analysis showed that porang had high diversity. The analysis of among variants clustering based on their molecules data showed that grouping fell into 3 clusters, which were: cluster I (JemberI, BlitarI and MadiunI variants), cluster II (Jember2, Blitar2 and Nganjuk2 variants) and cluster III (Blitar2 variant). The shortest distance was found between JemberI and BlitarI variants, while the furthest was found in Blitar2 variants.

Temperature Effect of Extraction on Physicalochemical Properties Glukomanan Origin Amorphophallus muelleri Blume Nunung Harijati; Serafinah Indriyani; Retno Mastuti
Natural B, Journal of Health and Environmental Sciences Vol 2, No 2 (2013)
Publisher : Natural B, Journal of Health and Environmental Sciences

This study had aims to find the optimum temperature for the extraction of the glucomannan with good physicochemical characters . The method that used for extraction was the stirring method according varies temperatures (35-55-75oC), followed by centrifugation with speed 1500 rpm for 30 minutes at temperatures 25°C. Glucomannan was coagulated using 95% isopropyl alcohol. Parameters that observed included glucomannan yield, ash and water content, flour color, lightness of glukomanan as well as calcium oxalate contents. The results showed that glucomannan extraction at 55°C gave optimal results (63.1% per dry weigh of tuber), followed by 52.2% and 39.91% for extracted temperature 35°C and 75°C respectively. There was no different significantly in color of glucomannan flour in between temperature which had L value 25.17; 27.23 and 26.37 correspond with extraction temperature 35°C, 55 °C, and 75°C. The calcium oxalate content of all treatment was 0.03-0,11% . The treatments also gave 1.17-2.00% ash content, 4.6-5.53% water content and 49.5-2654.5 Cp of viscosity.

Effect of Redox Time on Carbon Based Purity Made from Coconut Shell Simple Carbonization Zain Asrori; Lailatin Nuriyah; Djoko H Santjojo; Masruroh Masruroh
Natural B, Journal of Health and Environmental Sciences Vol 2, No 2 (2013)
Publisher : Natural B, Journal of Health and Environmental Sciences

Purification of carbon from coconut shell has been done by redox reaction method. Carbon was produced with simple heating carbonization method at 700 °C. Oxygen reduction process from the carbon was carried at temperature of 350 °C for 200 mL/min and 300mL/min with the H2 flow rate for 60 min, 120 min, and 180 min. Synthesis of carbon through simple heating carbonization method obtaining (93.84 ± 2.21) % of carbon content. Redox time optimization method successfully increased levels of carbon purity up (97.87 ± 1.47) %. The increase in carbon content was caused by the loss of a functional group with carbon-oxygen bond (C-O) and sulfur (S-O), as evidenced from FTIR test results.

Determination of Internal Structure of Semeru Volcano Based on Seismic Attenuation Imagery Hena Dian Ayu; Adi Susilo; Sukir Maryanto; Muhamad Hendrasto
Natural B, Journal of Health and Environmental Sciences Vol 2, No 2 (2013)
Publisher : Natural B, Journal of Health and Environmental Sciences

Characteristic of medium could be defined by physical parameters such as seismic velocity and attenuation. Amplitude, hyocentral distance, frequency and attenuation coefficient is required to determine the Q-factor value or attenuation in effort on imaging the internal structure. In this study, we used volcanic earthquakes data of Semeru Volcano recorded 2009 that consist 54 seismogram (24 A-type volcanic earthquakes and 24 explosion volcanic earthquakes. The Q-factor value of A-type volcanic earthquakes is 4 - 392 or about 78,87 and the Q-factor value of explosion volcanic earthquakes is 4 - 288 or about 51,39. In term of the image quality, it can be seen that the attenuation on Jongring Seloko active caldera and under Jongring Seloko have low attenuation value or high Q-factor value thas was called the zone beneath. The zone beneath is interpreted to associated with hot material that may indicate the magma chamber that concentrated in the depth interval of 1-1,8 km from the top of Semeru Volcano. The zone beneath have structural trends on West-East-Southeastern-South sector out the volcano. The internal structure of Semeru Volcano is predominance of sedimentary rocks, sandstone and rocks with gas in pore space. Although in some deep layer have internal structure as metamorphic rocks and igneous rock.

Study of Antibodies Expression with Specific Peptide Stimulation Specimens from gp350 / 220 (EBV) in Cell B Bambang Pristiwanto; Muhaimin Rifa’i
Natural B, Journal of Health and Environmental Sciences Vol 2, No 2 (2013)
Publisher : Natural B, Journal of Health and Environmental Sciences

Nasopharyngeal Carcinoma (NPC) is caused by Eipstein Barr Virus (EBV) which attack nasopharyngeal epithelium. EBV uses protein gp350/220 in the infection mechanism in nasopharyngeal epithelial surface receptor, CR2. The purpose of this study was to test the vaccine candidate at in vivo method. Mus musculus(Balb/C) mice were used as animal model. In this experiment mice pups in the age of 2 weeks were used. Injection using a specific peptide sequences from EBV showed higher expression of IgM (2.01%) compared to control (1.02%). Injection of this peptide to mice pups also increase the expression of IgM. After injection with this peptide, IgD also increase (75.74) compare to control (65.59%) without manipulation. In contrast, injection of this peptide decreases the expression of IgA antibodies (0.57%) significantly compare to control (1.93%).

Effect of Reduction Reaction Temperature on Carbon Purification based on Coconut Shell Marsi Bani; Djoko H Santjojo; Masruroh Masruroh
Natural B, Journal of Health and Environmental Sciences Vol 2, No 2 (2013)
Publisher : Natural B, Journal of Health and Environmental Sciences

The purification carbon from coconut shell through carbonization with pyrolysis method at temperature 600 °C for 1 hour has been done. Carbon is refined in various temperature such as 150 oC, 200 °C, 250 °C, 300 oC, and 350 °C. The flow rate of carbon refining is 200 mL/minute for 1 hour using a reduction reaction method with hydrogen as the reducer to reduce oxygen content in the carbon. The result show indicates that the maximum carbon content 96,53 % at 350 °C. The increasing carbon content during the reduction process because hydrogen attaches oxygen to establish H2O bond and it is proved by the result of FTIR test.

Distribution of Hot Springs Blawan-Ijen Based on Geoelectrical Resistivity Data Ika Karlina Laila Nur Suciningtyas; Sukir Maryanto; Arif Rachmansyah
Natural B, Journal of Health and Environmental Sciences Vol 2, No 2 (2013)
Publisher : Natural B, Journal of Health and Environmental Sciences

Geothermal energy is a source of heat that occurs naturally in the subsurface. One of the geothermal potential in East Java is in the Blawan that known as geothermal Blawan - Ijen. To find a geothermal potential of an area we have to do some research on subsurface conditions. Research using geoelectric resistivity method has been performed to estimate the distribution of hot springs. The study, conducted in May 2013 showed that there are 21 hot springs manifestations in Blawan - Ijen. Each of these hot springs have a temperature less than 50 °C. Data of geoelectric resistivity divided into 3 parts, resistivity values less than 40 ohm.meter indicated by the color blue , the color green for the resistivity value between 40-1280 ohm.meter and the color orange for values more than 1280 ohm.meter. Geothermal distribution that contained in Blawan is diffused follow existing fracture. Direction of hot springs are heading to the Northeast that following the river flow. The heat carrier layer is permeable layer that have resistivity values less than 40 ohm.meter.

Preliminary Study on the Determination of Volcanic Tremor Epicenter Using Semblance Method (Case Study of Sakurajima Volcano) Ratri Andinisari; Sukir Maryanto; Ahmad Nadhir
Natural B, Journal of Health and Environmental Sciences Vol 2, No 2 (2013)
Publisher : Natural B, Journal of Health and Environmental Sciences

Volcanic tremor is one of the most important volcanic activities due its strong connection to magma movement and impeding eruption. Therefore, the determination of volcanic tremor epicenter becomes very important. The absence of apparent P and S wave arrival of volcanic tremor is the main constraint of its epicenter determination. The use of semblance method is required because semblance method requires no information about P and S wave arrival in epicenter determination. A preliminary application of semblance method has been done to determine the epicenter of 100 s length volcanic tremor of Sakurajima volcano. In this research we use the recorded seismic signal from 5 different volcano observatories, which are KOM, KAB, HIK, ARI, and HAR. The recorded tremor signal is then analyzed by using semblance method. The analysis is carried out by calculated semblance coefficient in every 30 s moving window along the signal. The resulting semblance coefficient varied from 0,25 to 0,42. The epicenter of observed volcanic tremor is located in the north-east part of Minamidake crater by -18,66o to 54,10o of azimuth counted from the east, while the epicentral distance of the observed volcanic tremor varied from 608,28 to 948,68 m from Minamidake crater.

Effects of Turmeric (Curcuma domestica) in Maintaining Reduced Raw Cattle Protein Levels Unggul P Juswono; Johan A E Noor; Avika Dyah Respati
Natural B, Journal of Health and Environmental Sciences Vol 2, No 2 (2013)
Publisher : Natural B, Journal of Health and Environmental Sciences

A biological tissue that exposed by gamma radiation is known to be ionized that cause heavily damage due to the presence of free radicals. One of the damage may occured in the protein molecules which cause decreaseing of protein level of the beef so it needs a research on the effect of turmeric yellow (as antioxidant) in the maintaining of the protein level of the beef. In this research, the sample were meats without added turmeric as a control and the other were meats added with turmeric and then irradiated using radioactive elements Cs-137 and Co-60. Protein levels were tested using the standart method to analyze the levels of soluble protein. The results showed that the addition of turmeric had an influence to keep the protein value of beef. This situation suggests that turmeric is an antioxidant that is very good in counteracting free radicals.

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