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Study of Stearic Acid Layer (SA) Microstructure on Surface Quartz Crystal Microbalance (QCM) Sensors Nurramdaniyah Nurramdaniyah; Masdiana Padega; Djoko Herry Santjojo; Setyawan P. Sakti; Masruroh Masruroh
Natural B, Journal of Health and Environmental Sciences Vol 4, No 2 (2017)
Publisher : Natural B, Journal of Health and Environmental Sciences

In the development of QCM-based biosensor sensors, the sensor surface is a very important part because it determine how the surface interacting with its environment especially during the immobilization of biomolecules process. In the utilization of QCM as biomolecule detecting biosensor, it is necessary to coat the polymer material on the surface of QCM. A stearic acid layer is used as an immobilization matrix layer because stearic acid has both polar and non polar properties so it can interact with biomolecules. The stearic acid layer is deposited on the polystyrene surface using a vacuum evaporation method. A study of the microstructure of the stearic acid coating was conducted to determine the possibility of the deposited stearic acid layer used as the matrix layer. In this research, microstructural study of layers of the stearic acid by using Scanning Electron Microscope (SEM) was carried out to find out the surface morphology of stearic acid. X-Ray Difraction (XRD) measurements were conducted to identify the crystal structure of stearic acid while Fourier Transform Infrared (FTIR) measurements were used to determine the stearic acid functional group. The Scanning Electron Microscope (SEM) results show the surface of the cavity and pore layers of the polystyrene layer was coated with white fibers homogeneously deposited on the surface of QCM / Polystyrene. Furthermore, the X-Ray Difraction (XRD) results showing a spectrum peak at an angle of 2q = 26,66° indicates the existence of stearic acid crystals. Whereas, the measurement results of the Fourier Transform Infrared (FTIR) indicate the appearance of C-H functional groups at a wavelength value of 3600-2600 cm-1 which indicates the functionality of the stearic acid layer.

The Effect of Smoking Behavior in the Human Population Growth of Lung Cancer Patients Lu’luul Wardah; Trisilowati Trisilowati; Wuryansari Muharini Kusumawinahyu
Natural B, Journal of Health and Environmental Sciences Vol 4, No 2 (2017)
Publisher : Natural B, Journal of Health and Environmental Sciences

This article discusses a model of lung cancer as the effect of smoking behavior on both active and passive smoker. There are four subpopulations in this model, namely susceptible subpopulation, active smoker subpopulation, passive smoker subpopulation, and subpopulation of lung cancer. Dynamical analysis is conducted to determine the equilibrium point, existence condition for equilibrium point, and analyze their stability. Based on analysis result, there are three equilibrium points. First equilibrium point shows that all subpopulations extinct. Second equilibrium point shows that only susceptible subpopulation can survive, and the last equilibrium point shows that all subpopulations can survive. First equilibrium point always exists while the others exist under certain condition. The stability of first equilibrium point can be reached when the intrinsic growth rate is less than the death rate. Whereas, the others equilibrium points will be stable under certain condition. Numerical simulation is performed to illustrate the analysis result. It is shown that numerical results are in accordance with analysis result. These numerical simulations also indicate that the rate of passive smoker plays important role in the growth rate of lung cancer.

The Efffect of Wave Sound at Frequency 3 kHz - 4 kHz on Logawa Rice Resistance to Rice Lices Kuni Nadliroh
Natural B, Journal of Health and Environmental Sciences Vol 4, No 3 (2018)
Publisher : Natural B, Journal of Health and Environmental Sciences

Rice lice is one type of pest that attacks rice, the presence of rice lice can damage the content of rice, rice form and color of rice. Rice that is exposed to rice lice pests will easily fragile and turn into powder and the color of the rice turns yellowish. Innovation is needed to reduce the population of rice lice in the storage phase, so this study aims to determine the effect of sound waves of 3 KHz to 4 KHz frequency on the resistance of logawa varieties to rice lice, in this study the rice plants given the treatment of sound waves of 3 KHz frequency until 4 KHz and without sound waves as controls. Giving of sound waves is done every day from 06.00 to 09.00.

The Effect of Taurine on Superoxide Dismutase Enzyme Activity, Malondialdehyde And Histology In Liver Mice (Mus Musculus) Male-Induced Herbicide Glyphosate Elfa Verda Puspita; Gregorius Nugroho Susanto; Sumardi Sumardi; Endang Linirin Widiastuti
Natural B, Journal of Health and Environmental Sciences Vol 3, No 3 (2016)
Publisher : Natural B, Journal of Health and Environmental Sciences

Glyphosate herbicide widely used by farmers in Indonesia. As a result of exposure to herbicides, the production of free radicals or ROS exceeds the body's defense system called oxidative stress. Therefore, the activity of antioxidant enzymes in the body decline and thus can cause tissue damage and certain organ dysfunction. ROS can be suppressed by administration of antioxidants. One source of the antioxidant is taurine. Taurine has a protective effect against oxidation and free radical capture in a variety of cells and tissues against toxic oxidant component. This study aims to determine the effect of taurine in the liver of mice induced herbicide glyphosate on the activity of the enzyme superoxide dismutase (SOD), malondialdehida (MDA), changes in morphology and structure of liver histology. The research used completely randomized design (CRD). Mice were divided into three treatment groups such as, control group (K0), a group of glyphosate (K1) at a dose of 25 mg /kgBW/day orally for 25 days, and a group of taurine+glyphosate (K2) at a dose of taurine 7800 mg/kgBW/day + glyphosate dose 25 mg/kgBW/day orally for 25 days. Each treatment consisted of 8 mice as replication. Results of analysis with one ways ANOVA (P<0.05) followed by the Tukey test showed taurine dose of 7800 mg/kgBW/day can reduce liver cells of male mice that were treated by the herbicide glyphosate but it not be able to increase the levels of SOD and to decrease MDA levels.

Ch2FCF3 Gas Flow Rate Effects of SiO2 Plasma Etching Rate on Quartz Crystal Microbalance Antonius Prisma Jalu Permana; Dionysius Joseph Djoko HS; Masruroh Masruroh
Natural B, Journal of Health and Environmental Sciences Vol 3, No 4 (2016)
Publisher : Natural B, Journal of Health and Environmental Sciences

Effect of gas flow rate on the surface of quartz crystal SiO2 during plasma etching was studied. The etching process was applied using the capacitively coupled plasma (CCP) reactor generated with 100 W power AC generator at low frequency of 40 kHz using a gas flow of CH2FCF3. The main objective of this study was to determine the effect of changing gas flow rate on the plasma etching rate on the SiO2 surfaces. Gas flow rate was varied between 20-40 mL/min, while keeping all other plasma parameters constant. The etched surface of SiO2 was analyzed using white-light profilometer (Topography Measurement System (TMS 1200 Micro Lab)). The results show the physical etching processes influenced the rate of etching at a low gas flow rate, while the higher flow rate influence reactive ion etching (RIE) in the etching process. The maximum etching rate is found at 7.753 nm / min achieved in plasma etching process.

Utilization of Medicinal Plants in Kayukebek Village, Tutur District, Pasuruan Regency Agung Witjoro; Sulisetijono Sulisetijono; Frida Kunti Setiowati
Natural B, Journal of Health and Environmental Sciences Vol 3, No 4 (2016)
Publisher : Natural B, Journal of Health and Environmental Sciences

Traditional medicine using herbs has been known and done since the first generations by the people of Indonesia. Tutur area is located in the district of Pasuruan, East Java Province. One of the villages in the Tutur area is Kayukebek. The community Kayukebek have knowledge about the advantages of plants as a medicine for specific diseases or health problems. The study aims to: 1) To obtain information the types of plants used as a medicine; 2) To know the various uses of herbs; 3) To determine the perception and appreciation of the herbs from the Kayukebek community. This research is a descriptive explorative study. The method performed in the exploratory survey data collection by direct observation in the field and Participatory Rural Appraisal methods. Community engagement obtained through interviews with semi-structural and open-ended based question list. Sampling technique used is purposive sampling and snowball sampling. The value of the use of plants known by the value of Informant Concencus Factor and Use Value. The results, recorded 22 plants species have been used in this community as traditional medicine, include: garlic, tropong onion (scallion), plantain, dlingo, ceplukan, jambu wer, senggani, blencong, tamarind, kecubung, mustard greens, cabbage, fennel, turmeric, ginger, pulosari, sendokan, corn, white wood, sempretan (brojo lintang), gorogo, and wono ginger. It was observed that the local people are less aware of the medicinal plants. It is seen the public perception of the use medicinal plants is low (48.03%) meanwhile the appreciation medicinal plants are prefer to low category (49.69%).

Analysis of Experimental Results Measurement of Electric Impedance Value on Liquid with Injection Flow on Parallel Plate Gianita Anastasia Salamena; Abdurrouf Abdurrouf; Didik Rahadi Santoso
Natural B, Journal of Health and Environmental Sciences Vol 4, No 1 (2017)
Publisher : Natural B, Journal of Health and Environmental Sciences

This paper discusses the results of experimental measurement of the electrical impedance of liquids in parallel and perpendicular position to the direction of current injection. Experiments carried out on three different types of liquid i.e. distilled water, mineral water, and milk. Each liquid is placed in a container box with volume of 2,5cm x 2,5cm x 2,5cm. The electrode is located in the middle of the upright side of the container, forming two pairs of parallel plates, where one pair of parallel plate will serve as the current electrode. Current signal frequency range used in this experiment is 1Hz-500Hz. Experimental results obtained some things such as each liquid has a different electrical characteristic, distilled water has much larger electrical impedance compared to the mineral water and milk. Area of the electrode affects the measured impedance values on each liquid; the larger area of the electrode will decrease impedance value. The position of the electrode against the direction of flow injection showed the difference in impedance values measured, where the impedance measured at liquid injection electrode direct current is greater than the value of the impedance measured at liquid electrodes perpendicular injection current.

Activity Test Ni/ZrO2-SiO2 Catalyst for Hydrodeoxygenation Reactions Mixtures the Furfurylidene Acetone Compound (FAc) and Difurfurylidene Acetone (F2Ac) Dita Prihartini; Siti Mariyah Ulfa; Elvina Dhiaul Iftitah
Natural B, Journal of Health and Environmental Sciences Vol 3, No 3 (2016)
Publisher : Natural B, Journal of Health and Environmental Sciences

Hydrodeoxygenation (HDO) reaction of furfural derivatives have widely studied because these reaction produce saturated alkane which potensial for renewable fuels. Nickel based catalyst impregnated in to ZrO2-SiO2 (Ni/ZrO2-SiO2) was prepared and its activity is evaluated for HDO of furfurilidene aceto (FAc) and difurfurilidene acetone (F2Ac). Ni/ZrO2-SiO2 catalys was prepared from Ni(NO3)2.6H2O with 10% Ni loading over ZrO2-SiO2 by wet impregnation method. Characterization using X-ray showed the diffraction of Ni detected at 2θ = 44.42, 51.73, and 76.26. Catalyst activity for HDO of FAc and F2Ac was carried out at 200oC for 2 hours in batch system. According to gas chromatography analysis, the conversion of product is 100% with selectivity for 2-octanol and dodeka-6-ene is 88,22% and 2,99%, respectively.

Effect of Spirulina Extract on Diazinon Effect at the Channel Membrane Albumin and Vitelin Membranes Based on Strong Flows and Voltage Dian Rizqi Nur Amalia; Abdurrouf Abdurrouf; Unggul P. Juswono
Natural B, Journal of Health and Environmental Sciences Vol 4, No 2 (2017)
Publisher : Natural B, Journal of Health and Environmental Sciences

The use of pesticides in foods can be bad for human health, so the danger of pesticide use in food can damage cell metabolism or permeability of the cell membrane. This study aims to analyze the effect of Spirulina extract on the potential difference of egg cell membrane of albumin and vitellin chickens against anti channel blocker on diazinon. The contaminant used in this research is diazinon 600 EC. How to overcome the contamination of livestock products that can be harmful to health one of them using antioxidants. Thus, the administration of Spirulina extract can affect the potential response of egg cell membranes (Gallus Gallus domesticus) in the polluted food of diazinon. Potential rabbit egg membrane can be measured by using microelectrode associated with PicoScope 5000 series, then the data appears will be displayed through the PC. The results of this measurement showed that along with the increase of diazinon solution concentration, the potential value of egg cell membrane produced decreased. While the influence of Spirulina extracts in the egg (Gallus gallus domesticus) membrane that has been contaminated diazinon showed the potential value of membrane bigger/higher. This is because in the extract Spirulina has phenolic and flavonoid content that has the potential as an antioxidant that can be useful to reduce the level of free radical reactivity caused by diazinon.

Effect of Stirring Speed on Characteristics of Fe3O4 Nanoparticles Coated with Polyvinyl Alcohol and Glutaldehyde as a Crosslinker Rahayu, Lale Budi Hutami; Wulandari, Ika Oktavia; Santjojo, Djoko Harry; Sabarudin, Akhmad
Natural B, Journal of Health and Environmental Sciences Vol 4, No 3 (2018)
Publisher : Natural B, Journal of Health and Environmental Sciences

Research on Fe3O4 nanoparticles is mostly done because of the unique nature of Fe3O4 which has magnetic properties, non-toxic, biocompatible and easily degraded so it is interesting to investigate as a drug delivery system. This nanoparticles were successfully synthesized by coprecipitation method with variations of stirring rate were 180 rpm (sample A), 250 rpm (sample B) and 320 rpm (sample C). FTIR analysis of Fe3O4 nanoparticles, was absorption at ~585 cm-1 for samples A and B showing the presence of Fe-O magnetite bond and absorption at ~623 cm-1 which were not very sharp for maghemite. However the sample B, only absorption at ~623 cm-1 which is sharp for maghemite (Î³-Fe2O3) because of Fe2O3 cubic is not formed. SEM analysis did not show significant morphological differences in velocity variation. From XRD analysis known that the crystal size for samples A and B respectively of 6.4192 nm and 9.5513 nm for sample C could not be calculated because of many iron oxide phases present in it, so it is difficult to find FWHM. ESR analysis of nanoparticle samples shown Fe3O4 properties were ferromagnetic.

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Kota malang,

Jawa timur

INDONESIA