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Jurnal Biologi Universitas Andalas
Published by Universitas Andalas
ISSN : -     EISSN : 23032162     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
Arjuna Subject : -
Articles 4 Documents
 1 
Search results for , issue "Vol 9 No 2 (2021)" : 4 Documents clear
Kidney Histology of Wistar Rats (Rattus norvegicus Berkenhout 1769) Due to White Coffee Agi, Yola Alunsa; Titrawani, Titrawani
Jurnal Biologi Universitas Andalas Vol 9 No 2 (2021)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.9.2.60-67.2021

Abstract

Coffee is a drink that is very popular with the people of Indonesia and in other countries. Coffee has many benefits but on the other hand, coffee can also damage health, the dangerous content in coffee is caffeine and cafeeic acid. The effect of coffee consumption is still a matter of controversy, one of which is the effect on the kidneys because the kidneys are the main excretory organ for removing metabolic waste from the body. The kidney is the second organ after the liver, which is most often the target of damage by chemicals. This study aims to determine the effect of multilevel doses of white coffee on the histological picture of rats' kidneys. This research is a completely randomized design study with four 4 treatments and four 4 replications. Consists of K (distilled water), P1 (0.36 ml), P2 (1.08 ml) and P3 (2.16 ml). The results of this study showed that macroscopic observations of the administration of white coffee civet coffee solution with different doses showed that the kidneys looked normal. It is characterized by the shape of the kidney like a pea and brownish-red in color. The P0 group was significantly different from the P1, P2, P3 groups. The P1 group was significantly different from the P0 group and the P2 group, P3. The P2 group was significantly different from the P0, P1, and P2 groups. The P3 group was significantly different from the P0, P1, and P2 groups. This study concluded that white coffee in multilevel doses affects the histopathology of kidneys in rats.
Potential Valuation Of Plants Based On Utilization Of Plants, Local Wisdom and Local Resident Attitude about Plant Conservation in Silokek National Geopark Putri, Dina Hayati; Maideliza, Tesri; Mahdi, Mahdi
Jurnal Biologi Universitas Andalas Vol 9 No 2 (2021)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.9.2.68-75.2021

Abstract

The objectives of this study was to evaluate the utilization of plants, local wisdom and tribe attitudes about plant conservation by the people in Silokek National Geopark. This research used survey method. These data were collected by using Pebble Distribution Method (PDM) and Quisioner. And then, data had been calculated by calculating the value of Local User’s Value Index (LUVI). The result indicated that local people utilizing plants is for herbal medicine 83%. Cyclea barbata is spesies with having the highest LUVI (0.137), followed by Artocarpus elasticus used for furniture (LUVI= 0.060), Styrax sumatrana (LUVI= 0.126) used for ritual, and Imperata cylindrica (LUVI= 0.073) used for livestock feed. And The result showed at least three local wisdom that possible to be integrated to plant conservation such as planting the plants, selectively harvest by select cutting and timing, and special treatment before used the plants like shalawat nabi reading. For tribe attitudes indicated people in Silokek National Geopark more 90% agree for conservation policy in that area.Keywords : Local User’s Value Index (LUVI), Pebble Distribution Method (PDM), Silokek National Geopark.
RAPD Primers Selection for Genetic Variation Analysis of Banana Plant (Musa spp.) Wiguna, I Ketut Catur; Pharmawati, Made
Jurnal Biologi Universitas Andalas Vol 9 No 2 (2021)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.9.2.47-53.2021

Abstract

Identification of genetic diversity using molecular markers is important for basic information for plant conservation. Banana is a fruit bearing plants that important for food sources in human life. This study aimed to determine the optimum conditions of  PCR-RAPD reaction and RAPD primers that suitable to amplify DNA fragmens. DNA isolation was done using modified of CTAB and chloroform isoamil alcohol. The samples used was young leaves of nine banana cultivar plants. Optimation was done using variety of DNA and MgCl2 concentration. Eight primers produced by Operon Primer Technology were tested. The DNA genomic concentration obtained was in the range of 23,3 ng/µl – 70 ng/µl. The optimum conditions of PCR-RAPD of banana plants that produce clear band were 50 ng/µl DNA template, 3 mM MgCl2 with the number of thermal cycles was 40 x. There were six RAPD primers that successfully amplified DNA : OPA 02, OPA 04, OPB 12, OPD 20, OPH 01, and OPH 03. The primer OPA-04 had the lowest resolving power value (4,4) , while  OPH 01 had the highest (11,3) resolving power.
Characterization And Screening Of Protease, Amylase, And Cellulase From Phylloplane Fungi Isolates Of Avicennia marina (Forssk.) Vierh. Mahardhika, Wahyu Aji; Ramadhany, Warih; Lunggani, Arina Tri
Jurnal Biologi Universitas Andalas Vol 9 No 2 (2021)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.9.2.54-59.2021

Abstract

Mangrove (Avicennia marina) is an important mangrove species, because it has many benefits for human life, including in the ecological scope as a source of food and medicine. These plants can also be used as a source of symbiont mold isolates which can be developed as an alternative to produce bioactive compounds, one of which is enzymes. Enzymes are protein compounds that can catalyze all chemical reactions in biological systems. This study aims to determine whether mangrove symbiont fungi (A. marina) can produce amylase, protease and cellulase enzymes. This research was conducted using a method based on enzyme activity, namely amylase activity with lugol iodine staining, protease activity and cellulase activity with congo red staining. on agar media enriched with 1% skim milk, 1% starch and 1% CMC. The results showed that 4 isolates were able to show potential enzymatic activity to be developed as agents for enzyme production.

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