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INDONESIA
Indonesian Journal of Biotechnology
Published by Universitas Gadjah Mada
ISSN : 08538654     EISSN : 20892241     DOI : -
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology
The Indonesian Journal of Biotechnology (IJBiotech) is an open access, peer-reviewed, multidisciplinary journal dedicated to the publication of novel research in all aspects of biotechnology, with particular attention paid to the exploration and development of natural products derived from tropical—and especially Indonesian—biodiversity. IJBiotech is published biannually and accepts original research articles featuring well-designed studies with clearly analyzed and logically interpreted results. A strong preference is given to research that has the potential to make significant contributions to both the field of biotechnology and society in general.
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Articles 7 Documents
 1 
Search results for , issue "Vol 28, No 2 (2023)" : 7 Documents clear
Antibacterial activity of bioactive compound produced by endophytic fungi isolated from Mangifera casturi Kosterm endemic plant from South Kalimantan, Indonesia Fatia Rizki Nuraini; Ratna Setyaningsih; Ari Susilowati
Indonesian Journal of Biotechnology Vol 28, No 2 (2023)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.71150

Abstract

The endophytic fungi that live in endemic plants are a promising bio‐prospect as the producers of antibacterial compounds. This research is aimed to evaluate the endophytic fungi antibacterial compound from Mangifera casturi. The bioactive compounds of 13 endophytic fungi were extracted using ethyl acetate and evaluated for antibacterial activity using disk diffusion assay. The minimum inhibitory concentration (MIC) was measured using the serial broth dilution method. Scanning Electron Microscopy (SEM) was used to examine cell damage because of the extract. The antibacterial compounds were then detected using GC‐MS analysis. The endophytic fungi were identified morphologically and molecularly based on ITS rDNA sequencing Among the 13 isolates, the endophytic fungi identified as Botryosphaeria rhodina AK32 produced the antibacterial compounds that exhibited the highest activity and a broad spectrum. Moreover, they were capable against resistant bacteria, Methicillin Resistant Staphylococcus aureus (MRSA) with an MIC value of 1.56% for all the test bacteria. The mechanism of action of AK32 ethyl acetate extract seemed to affect the condition of bacterial cell walls, causing morphological alteration such as shrinkage of the cell, warted cells, and hollow cells. Based on GC‐MS, the antibacterial compounds of AK32 ethyl acetate extract were di‐n‐octyl phthalate, benzyl alcohol, high‐oleic (CAS) safflower oil, benzene acetonitrile, and benzotriazole.
In silico analysis of antibiotic resistance genes in Lactiplantibacillus plan‐ tarum subsp. plantarum Kita‐3 Angelia Wattimury; Dian Anggraini Suroto; Tyas Utami; Rachma Wikandari; Endang Sutriswati Rahayu
Indonesian Journal of Biotechnology Vol 28, No 2 (2023)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.72550

Abstract

The absence of transferable antibiotic resistance genes is required for the safety of commercial probiotics. Previous studies have found that antibiotic resistance genes on plasmids in Lactobacilli make them unsafe for food purposes due to the genes’ ability to transfer to pathogenic microorganisms. In contrast, bacteria from the Lactobacillaceae family are widely used as a probiotic. This study assessed the antibiotic susceptibility of Lactiplantibacillus plantarum subsp. plantarum Kita‐3 (previously known as Lactobacillus plantarum K‐3) isolated from Halloumi cheese using eight antibiotics. Genome sequencing was performed using the Illumina NovaSeq 6000 sequencing platform to detect the presence of antibiotic resistance genes on chromosomes and plasmids. L. plantarum subsp. plantarum Kita‐3 was resistant to clindamycin, streptomycin, and chloramphenicol but susceptible to tetracycline, ampicillin, kanamycin, erythromycin, and ciprofloxacin. Genome sequencing of L. plantarum subsp. plantarum Kita‐3 verified the presence of tetracycline, fluoroquinolones, β‐lactamase resistance genes, and multidrug resistance efflux. Kita‐3 had no transposable elements, gene transfer agents, plasmid‐related functions, or intact prophages. Overall, this study produced the antibiotic resistance profile of L. plantarum subsp. plantarum Kita‐3 to assess the risk of transferring antibiotic resistance genes to other bacteria. The study provides essential data on the safe use of L. plantarum subsp. plantarum Kita‐3 as probiotics.
Electrochemical performance of plant trace element incorporated silver nanoparticles synthesis from Datura metel L. Karuppiah Chinniah; Karthik Kannan; Vivek Maik; Vladimir Potemkin; Maria Grishina; Samuel Johnson Christopher Jeyaseelan; Arumugam Muthuvel; David Gnanasangeetha; Krishnamoorthy Gurushankar
Indonesian Journal of Biotechnology Vol 28, No 2 (2023)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.76257

Abstract

In our report, silver nanoparticles (AgNPs) were prepared from the leaf extract of Datura metel L. via the green synthesis method. Datura metel L. is a herbal medicinal plant from the Solanaceae family. The as‐prepared AgNPs were characterized using UV‐Vis spectrometer, X‐ray Diffraction (XRD), Fourier Transform Infrared (FTIR) spectroscopy and Scanning Electron Microscopy (SEM) with Energy Dispersive X‐ray (EDAX) analysis. The peak appearance of a Surface Plasmon Resonance (SPR) at 415 nm suggested the creation of AgNPs in the UV‐Vis spectrum. The XRD pattern showed the face‐centered cubic crystal structure of AgNPs with organometallic complex phase. Based on the FTIR spectrum, the peaks revealed the existence of biomolecules. SEM images showed the shape of the clastic rocks and the EDAX profile authenticated the presence of Ag and plant trace element. The cyclic voltammetry, Chronopotentiometry, and electrochemical impedance spectroscopy analysis were performed on an as‐prepared Ag electrode. A specific capacitance of 267.59 F/g at 0.5 A/g and a cyclic retention of 83.7% after 5000 charge‐discharge cycles were obtained. Hence, this material could be utilized in supercapacitor energy storage devices.
A novel pilot bioreactor for scaling up biomass and bioactive compounds on Gynura procumbens adventitious root culture Dannis Yuda Kusuma; Alfinda Novi Kristanti; Yosephine Sri Wulan Manuhara
Indonesian Journal of Biotechnology Vol 28, No 2 (2023)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.78420

Abstract

Bioreactors for adventitious root culture have been developed to obtain biomass and plant bioactive compounds in large quantities. These technologies provide a great opportunity to produce biomass and bioactive compounds more quickly from Gynura procumbens compared to conventional plant cultivation systems. In previous studies, biomass and bioactive compounds of G. procumbens adventitious roots were successfully increased using a small‐scale bioreactor. In this study, a pilot bioreactor the capacity of 19 L polycarbonate gallon was successfully developed. This bioreactor can be sterilized under the pressure of 0.18 MPa for approximately 60 min. While the bioreactor could not be sterilized when the pressure was less than 0.18 MPa damage may have occurred to the bioreactor vessel at pressures exceeding 0.18 MPa. The results of the chemical grade test as root culture media showed that MS‐Tek provided an optimal root biomass compared to MS‐PA after a 35‐day of the culture period. In addition, the productivity of the total phenolics and flavonoids of adventitious root in MS‐PA was higher than in MS‐Tek. This novel pilot bioreactor is suitable for G. procumbens adventitious root culture, and the technical‐grade chemicals are suitable for improving root biomass production.
Manufacturing and characterization of an effective and eco‐friendly nano insecticide with the aid of green alga Chlorella vulgaris in contrast to traditional insecticide Rasha Sattam Hameed; Raghad Jasim Fayyad; Maan Abdul Azeez Shafeeq; Rasha Saad Nuaman
Indonesian Journal of Biotechnology Vol 28, No 2 (2023)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.79105

Abstract

There is a growing demand for the manufacture of eco‐friendly insecticide. This study aimed to establish an aqueous extract of Chlorella vulgaris as a green factory to manufacture nano‐insecticide of titanium nanoparticles to control house flies (Musca domestica) by describing the basic properties of TiO2 solution before and after manufacturing. The absorbance was raised to 0.58, while transmission decreased to 38 under UV‐Visible spectra. Regarding to XRD analysis, seven sharp diffraction peaks appeared for a bulk solution while only three sharp peaks were noticed after phyco‐based synthesis. The crystal size of the prepared titanium nanoparticles was determined to be 27.39 nm. Furthermore, the observed size for bulk particles ranged from 92.33 to 249.6 nm through SEM, while for nanocrystalline the size ranged from 9.395 to 206 nm. Various phytochemicals were detected within the algal extract, including phenols, tannins, alkaloids, flavonoids, resins, and saponins. All of these active compounds participated in nano‐synthesis by acting as reducing and stabilizing agents. Finally, titanium nanoparticles were used as a controlling agent against house flies Musca domestica. In this study, this nanoparticles application also has been compared with traditional insecticide Imidacloprid. The high mortality percentages reached 100% against the first larval stage, 70% against the third larval stage, and 93.3% in adult flies. These mortalities were higher after using Imidacloprid for all tested stages. Many phenotypic distortions were also observed in house flies treated with TiO2 NPs prepared by Chlorella, including failure in pupal emergence and maturity, incomplete development in the head, legs, and wings, and disappearance of the genital organs. The study demonstrated that C. vulgaris is a good candidate for nanomanufacturing and a rich naturally derived nanopesticide.
Nephroprotective effects of cardamom essential oil (Amomum compactum Soland. Ex Maton) on kidney cells Nisa Ul Hasanah; Ahmad Syauqy Tafrihani; Ummi Maryam Zulfin; Dhiya Ulhaq Salsabila; Ratih Kurnia Wardani; Muthi' Ikawati; Edy Meiyanto; Riris Istighfari Jenie
Indonesian Journal of Biotechnology Vol 28, No 2 (2023)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.79642

Abstract

Many chemotherapeutic agents cause various side effects, including nephrotoxicity. Cardamom essential oil (Amomum compactum Soland. ex Maton) contains compounds that exhibit antioxidant activity, such as 1,8‐cineole, α‐pinene, α‐terpineol, and linalool. This study focused on exploring the potency of cardamom essential oil (CEO) as an anti‐senescent induced by doxorubicin using the Vero kidney cell line. We first obtained the CEO by steam distillation, then evaluated its cytotoxicity using a trypan blue exclusion assay. Moreover, we performed senescence‐associated beta‐galactosidase (SA‐β‐gal) staining and 2’,7’‐dichlorodihydrofluorescein diacetate (DCFDA) staining to measure the effect of CEO on intracellular ROS level and cell senescence, respectively. Analysis of the compounds with gas chromatography‐mass spectrophotometry (GC‐MS) revealed seven compounds with significant abundance, namely 1,8‐cineole (50.82%), ß‐pinene (12.43%), α‐terpineol (8.50%), fenchone (4.10%), α‐pinene (4.00%), sabinene (3.00%), and linalool (1.98%). The cytotoxicity assay of CEO on Vero cells showed an IC50 value of 178 μg/mL. Thus, CEO is considered low cytotoxic for normal kidney cells (>100 μg/mL). Concentrations of 50 and 100 μg/mL CEO reduced the cell senescence induced by doxorubicin. Therefore, CEO has potency as a nephroprotective agent in doxorubicin‐induced senescence.
Genetic variation and genomic constitution in orchid Dendrobium hybrid section Spatulata derived from interspecific hybridization based on sequence related amplified polymorphism marker Aziz Purwantoro; Agus Budi Setiawan; Rikcy Setiaji Nugraha; Sairoh Bisirotil Mujtaba; Aditya Hanung Setyadi
Indonesian Journal of Biotechnology Vol 28, No 2 (2023)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.83595

Abstract

Dendrobium hybrid section Spatulata is widely cultivated in Indonesia due to its ease of cultivation, high economic value and adaptability, also extended flower shelf life. Various attempts to meet the rising market demand for Dendrobium hybrid section Spatulata, including the development of new varieties with unique flower traits such as flower color, a longer and bigger horn, and disease resistance. In this study, we conducted a breeding program aimed at developing a new cultivar of Dendrobium hybrid section Spatulata (antelope orchids) through interspecific hybridization. The study aimed to investigate the genetic variation and genomic constitution of the eight hybrids and their corresponding parental lines that resulted from interspecific hybridization using sequence‐related amplified polymorphism (SRAP) marker. Six species of Dendrobium section Spatulata i.e., Dendrobium Sri Mulyani, D. Cochliodes, D. strepsiceros, D. stratiotes, D. Alice Noda, D. helix, and several hybrids of antelope orchids derived from three hybridizations including D. Sri Mulyani × D. cochliodes, D. stratiotes × D. strepsiceros, and D. Alice Noda × D. helix, respectively, were subjected into SRAP markers for genotyping analysis. Dendrobium hybrid section Spatulata hybrids produced by interspecific hybridization were genuine hybrids with substantial genetic variability based on flower morphology, including labellum shapes and color intensities, as well as curly horn shapes and color intensities. The SRAP marker, which was used to genotype the hybrid and parental lines, exhibited a significant degree of polymorphism, and might be used to distinguish each accession. It produced a unique DNA amplicon that ranged from 180 to 530 bp and inherited a certain progeny line. The unweighted pair group mean average (UPGMA) dendrogram and Principal Coordinate Analysis (PCoA) biplot showed that all the hybrids were grouped into three major clusters according to their corresponding parental lines based on their genetic background and ge‐ nomic constitution. These findings are critical for the genetic improvement of the Antelope orchid to develop novel varieties.

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