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Bioeksperimen: Jurnal Penelitian Biologi
Published by Universitas Muhammadiyah Surakarta
ISSN : -     EISSN : -     DOI : -
Core Subject : Education, Agriculture,
Agriculture, Biological Sciences & Forestry Education
Arjuna Subject : -
Articles 9 Documents
 1 
Search results for , issue "Vol 10, No 1: March 2024" : 9 Documents clear
Exploration of nyamplung rizosphere nitrogen fixing bacteria in mine reclamation Sriwulan Sriwulan; Tita Dewi Fitriani; Nadia Puspita Maya; Ni'matur Rohmah; Eko Purnomo
Bioeksperimen: Jurnal Penelitian Biologi Vol 10, No 1: March 2024
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v10i1.23011

Abstract

Nitrogen fixing bacteria have the ability to utilize free nitrogen as a nitrogen source for their growth. These bacteria are able to make efficient availability of N- in the soil, so that it can be utilized by plants. The aim of this study was to obtain nitrogen fixing bacteria isolates from the rhizosphere of nyamplung plants in limestone reclamation lands. Isolation and testing of nitrogen fixation ability by nitrogen fixing bacteria was carried out using selective Ashby's Mannitol Agar media. The isolation results obtained 5 isolates on Ashby's Mannitol Agar media. The morphological characters of the colonies of the five isolates showed round and irregular shapes, flat and convex elevations with flat edges, white, cloudy white and clear. Microscopic observation showed that the isolates consisted of gram-positive and negative bacteria with bacilli cell shape. The catalase test showed 4 positive isolates and 1 negative. The conclusion of this study was that 5 nitrogen fixing bacteria isolates were obtained from the rhizosphere of the nyamplung limestone land plant. Isolates 1, 3, and 5 showed the character of the genus Clostridium, isolate 2 led to the genus Bacillus, and isolate 4 had the same character as the genus Klabsiella.
Diversity of Crustaceans Family: Portunidae in East Coast Mangrove Forest Reserve Mendahara Resort Tanjung Jabung Timur Wafiq Nadya; Mahya Ihsan; Tia Wulandari
Bioeksperimen: Jurnal Penelitian Biologi Vol 10, No 1: March 2024
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v10i1.23163

Abstract

Crustacea, the largest subphylum in the Phylum Arthropoda, inhabits fresh and seawater. Their bodies are divided into three segments namely cephalo, thorax, and abdominal, or cephalothorax in some crustacean species and they breathe through gills. Crabs are one of the biota that can function as aquatic bioindicators in addition to making a major contribution to nutrient recycling. In the muddy parts of the mangrove forest, the decapod crustacean species Portunidae plays an important ecological function in the environment. This research aims to categorize the crustaceans of the Portunidae family in the East Coast Mangrove Forest Nature Reserve and calculate their dominance, uniformity, and diversity indices. The diversity, uniformity, and dominance index of the Crustacean Family Portunidae in the East Coast Mangrove Forest Reserve area was calculated in this quantitative descriptive research. Purposive sampling is a sampling approach used in survey methods used for sample collection. The results showed that the two different types of crustaceans collected for this study were Thalamita crenata (4125 individuals) and Scylla olivacea (a total 19 individuals). This research shows that in the East Coast Mangrove Forest Reserve area, Thalamita crenata is the most commonly found crustacean species in the Portunidae family. The overall dominance index is in the medium group, while the overall diversity and uniformity index is in the low group. The results of Principal Component Analysis (PCA) show that the environmental factor with the closest relationship and positive correlation between species diversity and uniformity and environmental parameters is salinity.
Cytotoxic Effects of Plant Bioactive Compounds on T47d Breast Cancer Cells Line: A Review Tio Firmansyah; Utami Saraswati; Kurniadi Ilham; Robby Jannatan; rita maliza
Bioeksperimen: Jurnal Penelitian Biologi Vol 10, No 1: March 2024
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v10i1.23534

Abstract

The continuous cell line known as T47D cells originates from the isolation of epithelial cells from the ductal breast tissue of a 54-year-old woman with metastatic breast cancer. Numerous plant species have been employed in alternative anti-breast cancer therapy, showing advancements in recent years. The aim of this systematic review is to assess the potential bioactive compounds from herbal plant extracts and analyze the effects of herbal plants on T47D breast cancer cells. This research employs a systematic approach to gather articles, encompassing related research questions, chosen databases, keyword selection, inclusion and exclusion criteria, as well as outcomes and conclusions regarding the collected articles. From the reviewed articles, several plants exhibit anti-breast cancer activity on T47D cell lines, namely Calamintha incana, Sterculia quadrifida, Psidium guajava, Uncaria nervosa, Syzygium aqueum, Capsicum annum, Bauhinia scandens, Lansium domesticum, and Garcinia cowa due to their content of bioactive compounds such as triterpenoids, alkaloids, phenolics, flavonoids, saponins, and tannins. These bioactive compounds from the plants exert significant influence on cell cycle, proliferation, fatty acid synthesis, and apoptosis of cancer cells.
Local Knowledge of The Community in Mekarwangi Village on Long-Tailed Macaque (Macaca fascicularis) Canaya Vania Rehansha; Rusdi Hasan
Bioeksperimen: Jurnal Penelitian Biologi Vol 10, No 1: March 2024
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v10i1.23855

Abstract

Local knowlede is a phenomenon that is felt and experienced by the people of a particular area who have local understanding regarding the natural resources found in the surrounding environment. One of this knowledge is about long-tailed macaque (Macaca fascicularis). This research aims to examine local knowledge of the community regarding long-tailed macaque in Mekarwangi Village which borders Taman Hutan Raya Ir. H. Djuanda which is one of the long-tailed macaque conservation sites. The method used in this research is a qualitative approach through observation, semi-structured interviews, and strengthened by literature study. The result obtained were analyzed descriptively. The reseach result show that the community knows about the existence of long-tailed macaque. Community interactions with long-tailed macaque pose a threat to long-tailed macaque, some individu of long-tailed macaque be arrasted because people are disturbed by their existance. The majority of community in Mekarwangi Viilage know the food, habitat, and daily activities of long-tailed macaque from daily observation and some knowledge passed down from previous generations.
Chemical Profiling of Essential Oil Extracted from Fresh Walang Leaves (Etlingera walang (Blume) R. M. Sm.), an Indigenous Species in Banten, Indonesia Cory Novi; Keukeu Listia Apriliane; Swastika Oktavia
Bioeksperimen: Jurnal Penelitian Biologi Vol 10, No 1: March 2024
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v10i1.22998

Abstract

Essential oils are a group of secondary metabolite compounds that have a distinctive aroma and are volatile. Walang leaves are one of the plants suspected of having essential oil content. This study aims to determine the constituent components of essential oil compounds from fresh walang leaves. The method used to extract essential oil from fresh walang leaves is steam distillation. The essential oil produced was analyzed using Gas Chromatography Mass Spectroscopy (GC-MS). The results showed that the essential oil of fresh walang leaves contained 15 components of essential oil compounds and 2 main components of essential oil compounds, namely: Acetic acid (CAS) Ethylic acid 24.52%, 1,2 Benzenedicarboxylic acid, bis(2-methylpropyl) ester (CAS) Isobutyl phthalate 21.09%.
Activity Test of Catalase Enzyme in Rhizospheric Soil Bacteria Berliana Putri Susanti; Apreza Eka Yuliani; Ahmad Fauzan Hidayatullah; Sutrisno Sutrisno
Bioeksperimen: Jurnal Penelitian Biologi Vol 10, No 1: March 2024
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v10i1.22957

Abstract

This research was conducted at the Microbiology Laboratory of UIN Walisongo Semarang to identify and analyze the catalase enzyme in rhizosphere bacteria around the Laboratory of Campus 2 of UIN Walisongo Semarang. This research was carried out in several stages, namely isolation of rhizosphere bacteria, characterization of rhizosphere bacteria, gram staining of rhizosphere bacteria, and catalase test of rhizosphere bacteria. The data analysis technique used is the descriptive data analysis technique. The results showed that 9 isolates of rhizosphere bacteria produced catalase enzyme in the presence of gas bubbles when reacted with H2O2.
Nutritional Benefits of Black Soldier Fly Larvae Oil in Animal Feed Rudy Agung Nugroho; Retno Aryani; Esti Handayani Hardi; Hetty Manurung; Rudianto Rudianto
Bioeksperimen: Jurnal Penelitian Biologi Vol 10, No 1: March 2024
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v10i1.23015

Abstract

Black Soldier Fly Larvae (BSFL) oil is currently an insect oil that potentially replaces fish oils in animal feed. However, there are limited information on its benefits and nutritional comparison of BSLF oil to fish oil. Present study was to evaluate the benefits of BSFL oil in animal feed and compare the BSFL to Fish oil. Collected data of BSFL oil in the animal feed and its physiological effects was obtained from several articles of  reputable journal and compiled into a table. Meanwhile, BSFL was reared and extract their oil to determine the fatty acid composition and compare to fish oil. The current study indicated that BSFL oil can be used in various animal feeds, such as aquafeed, broiler feed, and pets. The BSFL oil which is included in the feed showed an improvement in growth and other physiological responses of animals. Meanwhile, in comparison to fish oil, BSFL oil has a similar composition of fatty acids such as Omega 3 and Omega 6. Eicosapentaenoic acid (EPA; 20:5n3) is detected in the BSFL oil, though the quantity is much lower than in fish oil.  Further, the BSFL oil also contains lauric acid. In conclusion, BSFL oil can be an alternative feed ingredient to substitute fish oil and has beneficial to the growth and physiological responses of the animal.
Potential of Bioactive Compounds in Hepatotoxicity Using Primary Cell Culture Method: A Systematic Review Angga Illahi Kurnia Illahi; Afdhal Raihan; Ivan Petrovsky; Rita Maliza
Bioeksperimen: Jurnal Penelitian Biologi Vol 10, No 1: March 2024
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v10i1.23548

Abstract

Hepatotoxicity is a condition characterized by liver cell damage caused by toxic chemicals. This article aims to explore the effects of compounds that contribute to mitigating hepatotoxicity, focusing on in vitro studies, particularly primary cell culture. In this systematic review, we conducted searches in the PubMed, Science Direct, and Google Scholar databases to find articles published between 2008 and 2022. Several active compounds were identified, including N-Benzylpiperazine (BZP), 1-(3-trifluoromethylphenyl) piperazine (TFMPP), antimycin A, coenzyme Q (CoQ) substrates, the antioxidant Vitamin C, L-glutamine (Gln), Nimesulide, Luteolin, glutathione, angelica sinensis polysaccharide (ASP) from Angelica sinensis, ammonium glycyrrhizin (CAG) from Glycyrrhiza glabra, L-arginine, silymarin from Silybum marianum, and glucurolactone (GA). However, only six compounds were shown to have remedial and treatment effects on hepatotoxicity and utilized primary cell culture with MTT Assay. These six compounds are Luteolin, Glutathione (GSH), Angelica sinensis polysaccharide (ASP) from Angelica sinensis, Ammonium glycyrrhizin (CAG) from Glycyrrhiza glabra, Antimycin A (AA), and Glutamine (Gln). The mechanism of action of these compounds involves preventing further damage to liver cells and repairing cells that have already been damaged. In summary, these compounds play a significant role in addressing hepatotoxicity caused by toxic substances and drugs.
Toll-Like Receptor Activation and B Cell Maturation Via MyD88-Dependent Pathway Under Hyperglycemia Condition Riandini Aisyah; Safari Wahyu Jatmiko
Bioeksperimen: Jurnal Penelitian Biologi Vol 10, No 1: March 2024
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v10i1.23328

Abstract

hyperglycemia causes a degenerative syndrome that involves an inflammatory process with an increase in certain proinflammatory cytokines and chemokines which in the process will activate B cells to produce immunoglobulins through several mechanisms. One of the interesting mechanisms is the mechanism via the MyD88 pathway. Objectives: to explore the role of MyD88 adapter protein in Toll-like receptor activation and B cell maturation under hyperglycemia conditions. Methods: a literature review was done to answer the study objectives. Results: Class switching process under hyperglycemia conditions involves activation of NFkB through the inflammatory MyD88-dependent pathway to trigger the expression of TLR and B cell maturation and proliferation as well as antibody production. The MyD88 adapter protein is a protein formed by stimulation of pro-inflammatory cytokines IL-6 and plays a role in the continuation of signals from the TLR and IL-1 pathways. Mature B cell stimulation induces 2 genetic changes in the Ig gene locus, called somatic hypermutation (SHM) and class switch recombination (CSR) to produce antibodies. Changes in immunoglobulin genes occur related to changes in certain DNA segments at the locus of genes where CSR occurs, this gene change requires the role of AID (activated-induced cytidine deaminase) in DNA cleavage. AID in mature B cells is activated by proinflammatory cytokines via induction of NfKB activation via the inflammatory MyD88-dependent pathway. Conclusion: Toll-like receptor activation plays a crucial role in B cell maturation activated by pro-inflammatory cytokine via MyD88 dependent-NFkB activation. 

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